Supplementary Table S1: Bacterial Species and Strains Used in This Study

SupplementaryMaterial

Figure S1 A

Figure S1 B

Figure S1 C

Figure S1. Amplification curves for multiplex real-time PCR diagnostics assay. Fig.S1A Real-time amplification curves for 3 strains of P. aeruginosa (DSM 50071T, DSM 19880, DSM 1117) (triangles) targeting the ssrA gene in the HEX channel (523-568 nm); Fig S1B Amplification curves for the Burkholderia genus (circles) targeting the rnpB gene in the FAM channel (483-533 nm); Fig. S1C Amplification curves for the IAC in TYE665 channel (615-670 nm). All samples detected except the non-template control sample (diamonds).

Figure S2 A

Figure S2 B

Figure S2.Sensitivity of the P. aeruginosa specific diagnostics assay.Fig.S2A Real-time amplification curves showing sensitivity (serial dilutions of P. aeruginosa DSM 19880 DNA from 106 GE to 10 GE) of the P. aeruginosa specific diagnostics assay; Fig.S2B Standard curve representing above serial dilutions.

Supplementary Table S1: Bacterial species and strains used in this study.

a*DSM = The German Collection of Microorganisms; * LMG=LaboratoriumvoorMicrobiologie -Belgian Co-ordinated Collections of Micro-organisms

bPVRS= Pathovar reference strain

Supplementary Table S2: Other species and strains of microorganisms used in this study.

a *DSM=German Collection of Microorganisms and cell cultures; * LMG=LaboratoriumvoorMicrobiologie- Belgian Co-ordinated Collections of Micro-organisms; *NCTC National Collection of Type Cultures; *ATCC American Type Culture Collection; CBS= CentraalbureauvoorSchimmelcultures, Netherlands.

b Information not available (NA)

Supplementary Table S3: Accession numbers of P. aeruginosa strains and Burkholderia species/strains.