Supplementary Table S-2 Primers used for qRT-PCR verification of 45 genes identified as differentially expressed by microarray. Reference Genes used in this study are indicated by an asterisk (*). EST DV665120 corresponds to POP.
GenBank Acc# / Forward primer (5’-3’) / Reverse primer (5’-3’) / Amplicon length (nt)BQ449012 / AGTGGCAAGACTACAGCAATC / GTATACTGGAGAGGGCATGG / 260
DV664953 / AGAAAGGAAAGGCTGCCAAG / CATCAGCCAGTCTCTTGTTGTAAG / 258
DV665104 / GGCAAATTGCTGGTAAATGG / AATGCACCAGCAGTCGTAGTC / 260
DV665120 / TCAGCACCAACCCAATCATC / CCCAGAAAGTATCCACTGATCC / 260
DV667676 / GTGGTACCTGGTGGTGATTTG / TCGTACTCATCTTCCTCACCTTC / 260
DV673104 / CCATTTGTGCGAATGGATAG / CCAATTCCTGCTCATGCTTAG / 256
DV673873 / ATTTGGCTGGGACTGTGAAG / AATTCCACCAAATCGTGGTC / 259
DV681693 / GCAAGATCTCTTAGATGCCACTC / CTTCAAGCGGACGTTCTTC / 233
DV683054 / CCCTTGGAGAAGGACGATAG / GCTTGTTGAAAGGCCAAAG / 250
DV683068 / ACTCCACTCGGCCCATAAAG / ACTAAAGGCAACTGCGAACG / 260
DV687996 / GATTTGGTGCATGGTTGG / AATACCTCGTTCTCCCAAGG / 260
DV690720 / CTGTCCACAACTTCCCAAATC / AAGTTTCGACGTCCATGTCAG / 247
DV690901 / ACCGGAAGGTCCTTTCCTTAC / AAACACTGCAAACCCACCTC / 247
DV691520 / TCCTTTACCCATTCCTCACG / CCCGTATACAGACAACGGATAAC / 260
DV691687 / GCAATTGCTTGTGCCAAC / CCTCTACAGCCTGGATGATTG / 259
DV693274 / ACCTCCAGAATGGCTTGATG / CACAAGCTGCACCACAAATAAG / 259
DV696556 / TGCCTGTGCTGATTATTCAAGG / AAATCCCGTTTGCCTCCAC / 255
DV704564 / GGGCTTGGTGGACTACTTTG / AGTCAACATTGCGCATTCC / 260
DV704794 / GCATCAGTTGGTGGAGAATG / GGAAGTAAGTGGCGCAAATC / 259
DV705287 / TTTCATGGATGACAGGTAGCC / ACACCAGAGCATTAGGACTGC / 260
DV705403 / GGGCTTGGTGGACTACTTTG / AGTCAACATTGCGCATTCC / 260
DV705655 / TACCCAGGTGTTAAGAGGATCAC / GCAGCAACCTTCTCATCAAG / 260
DV708052 / GTTCTCCATTCTACGGACAGC / CTCTTTACAAGGTTGGAGATGC / 260
DV708812 / TGGAGGAGGAGAAACACCAC / ATGGAATGCAAATCCACCTG / 260
EE191879 / CGTCGAACTCGTTGTTAGAGC / CATCTCTACTCCCTGTTGTTTGG / 259
EE197127 / AACCATCCAGGACATTGAAG / AGCCATGAGATGCTGAGTG / 260
BQ448915 / GATCTGAGGTCCCTGTTGTTC / CCTTCCTTCTCTCTCGTGTTG / 233
GR980998 / ATTAATCGTCCCTGGCAATG / GCTAGCTTGATTGGCCTGAC / 218
*Ubiquitin Q (UbiQ; DQ123983.1) / TGTCCTGAATCTTTGCCTTGAC / TCCAAGACAAGGAGGGCATC / 237
*Glyceraldehyde 3-phosphate dehydrogenase
(GAPDH; DV692958.1) / GGCTGTTGGGAAAGTTCTTC / AATACCCTGCCTTAGCATCG / 260
*Phosphoglycerate kinase (PGK; DV666796.1) / GTGGCAAATCCCAAGAAGC / GCAACTACATCAGTGGGAAGC / 260
*Cyclophilin A (Cyclo A; DV702502.1) / TGGCAAAGCAGTTCCTAAGAC / CACCAGCATTAGCCATTGAC / 260
*Triose-phosphate isomerase (TPI;DV692214.1 ) / TCAACTCTGGAAGTTGTTGCTG / GCTTGTGCAGCTAATTCCTTG / 260
*Actin (Act; DV713342.1) / GTATGTTGCCATCCAAGCTGT / GGCAAGGCATAACCCTCATA / 120
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