Supplementary Table 4aAbnormal FISH results for a probe panel investigating the 4 loci frequently involved in CLL

ID / 17p13 / 11q22-23 / Centromere 12 / 13q14 / Reference
P53 / ATM / D12Z3 / Probe / Result / M/B / Cryptic
1 / Nl / [87/200] / Nl / LSI RB1, LSI D13S319 / [160/200] / M1 / Yes / All probes from Abbott b
2 / Nl / [130/200] / Nl / LSI RB1, LSI D13S320 / [139/200] / M / Yes / All probes from Abbott b
3 / Nl / Nl / Nl / LSI RB1, LSI D13S319 / Nl / Nl / Nl / All probes from Abbott b
4 / Nl / [81/100] / Nl / LSI D13S319, LSI 13q34 / Nl / Nl / Nl / All probes from Abbott
5 / Nl / [66/200] / Nl / LSI RB1, LSI D13S319 / Nl / Nl / Nl / All probes from Abbott b
6 / Nl / Nl / NA / NA / NA / NA / NA / All probes from Abbott
7 / Nl / Nl / [35/200] / LSI D13S319, LSI 13q34 / Nl / Nl / Nl / All probes from Abbott
8 / Nl / Nl / [107/200] / LSI D13S319, LSI 13q34 and
13q14.3, D13S1825 (LPH006) / Nl / Nl / Nl / LPH006 from Cytocell, all other probes from Abbott
9 / [159/200] / Nl / Nl / LSI RB1, LSI D13S319 / Nl / Nl / Nl / All probes from Abbott b
10 / Nl / Nl / Nl / LSI D13S319, LSI 13q34 / Nl / Nl / Nl / All probes from Abbott
11 / Nl / Nl / Nl / ON DLEU (13q14) / 13 qter / [77/150] / M2 / Yes / P53/D17Z1 from Cytocell, D12Z3 from Abbott, other probes from Kreatech
12 / Nl / Nl / NA / NA / NA / NA / NA / All probes from Abbott
13 / Nl / Nl / [120/200] / LSI D13S319, LSI 13q34 / Nl / Nl / Nl / All probes from Abbott
14 / [19/206]c / [103/206] / Nl / LSI RB1, LSI D13S319 / Nl / Nl / Nl / All probes from Abbott
15 / [122/200] / Nl / Nl / LSI RB1, LSI D13S319 / Nl / Nl / Nl / All probes from Abbott b
16 / Nl / Nl / [18/200]c / LSI RB1, LSI D13S319 / [29/200] / B / Yes / All probes from Abbott b
17 / Nl / Nl / [128/200] / LSI D13S319, LSI 13q34 / Nl / Nl / Nl / All probes from Abbott
18 / [192/200] / Nl / Nl / LSI D13S319, LSI 13q34 / [18/200]c / M1 / Yes / All probes from Abbott
19 / NAc / Nl / Nl / LSI D13S319, LSI 13q34 / Nl / Nl / Nl / All probes from Abbott
20 / Nl / Nl / Nl / LSI D13S319, LSI 13q34 / [34/200] / M1 / Yes / All probes from Abbott
21 / Nl / Nl / Nl / ON DLEU (13q14) / 13qter / Nl / Nl / Nl / All probes from Cytocell, except ON DLEU (13q14) / 13qter from Kreatech
22 / [183/200] / Nl / Nl / LSI D13S319, LSI 13q34 / Nl / Nl / Nl / All probes from Abbott
23 / [157/200] / Nl / Nl / LSI RB1, LSI 13S319 / Nl / Nl / Nl / All probes from Abbott b
24 / Nl / Nl / Nl / LSI D13S319, LSI 13q34, / [17/100] / B / No / All probes from Abbott
25 / Nl / [180/200] / Nl / 13q14.3, D13S1825 (LPH006) / [186/200] / M3 / No / LPH006 from Cytocell, all other probes from Abbott
26 / Nl / [87/100] / Nl / LSI D13S319, LSI 13q34 / [97/100] / M1 / Yes / All probes from Abbott
27 / Nl / Nl / Nl / LSI D13S319, LSI 13q34 / [108/200] / M1 / Yes / All probes from Abbott
28 / Nl / Nl / Nl / LSI RB1, LSI D13S319 / [78/200] / B / Yes / All probes from Abbott b
29 / Nl / [161/200] / Nl / LSI RB1, LSI D13S319 / [158/200] / M1 / Yes / All probes from Abbott b
30 / [8/200]a / Nl / Nl / LSI RB1, LSI D13S319 / Nl / Nl / Nl / All probes from Abbott b
ID, patient identification; Abnl, abnormal; Nl, normal; NA, not available; M, monoallelic deletion of both RB1 and D13S319, M1,monoallelic deletion of D13S319 only; M2, monoallelic deletion of DLEU1 only; M3, monoallelic deletion of D13S319 and D13S25; B, biallelic deletion; Abbott, Vysis/Abbott, Downers Grove, IL, USA; Cytocell, Cytocell Cambridge, UK; Kreatech, Kreatech, Amsterdam, The Netherlands
aNormal result (cut-off at 10%)
bExcept for the home made probe CEP17
cResult considered abnormal,as the abnormality was confirmed on metaphases.

Supplementary Table 4b FISH results for CCND1 in cases with PLL

ID / Probe / Result / Investigated metaphases (n) / Investigated nuclei (n) / Reference
7 / M-FISH
IGH/CCND1 / No t(11;14)
No CCND1 rearrangement / [10]
NA / NA
[200] / Metasystems
Metasystems
8 / IGH/CCND1 / No CCND1 rearrangement / NA / [200] / Metasystems
12 / LSI IGH/CCND1 / No CCND1 rearrangement / [20] / [100] / Abbott
13 / LSI CCND1 BA / No CCND1 rearrangement / NA / [200] / Abbott
15 / LSI CCND1 BA / No CCND1 rearrangement / [10] / [200] / Abbott
18 / LSI CCND1 BA / No CCND1 rearrangement / [20] / [200] / Abbott
21 / LSI CCND1 BA / No CCND1 rearrangement / [20] / [200] / Kreatech
22 / LSI IGH/CCND1 / No CCND1 rearrangement / [4] / [300] / Abbott
23 / LSI CCND1 BA / 7/15 metaphases were incomplete and show loss of 1 fusion signal
All nuclei showed 2 normal fusion signals / [15] / [200] / Abbott

ID, patient identification;n, number; BA, break apart; NA, not available/not applicable; Metasystems, Metasystems, Altlussheim, Germany; Abbott, Vysis/Abbott, Downers Grove, IL, USA; Kreatech, Kreatech, Amsterdam, The Netherlands