Supplementary Table 4.Top predicted molecular and cellular functions, canonical pathways, molecules, and hepatotoxic functions affected by Mid-PFNA + Mix exposure. Values for Mid-PFNA are presented for comparison.
Molecular and cellular functionsMid PFNA + Mix / Mid PFNA
Name / p-value / # molecules / p-value / # molecules
Energy production / 2E-23 - 2E-2 / 30 / 2E-24 - 3E-02 / 33
Lipid metabolism / 2E-23 - 2E-2 / 58 / 2E-24 - 3E-02 / 59
Small molecule biochemistry / 2E-23 - 2E-2 / 66 / 2E-24 - 3E-02 / 71
Nucleic acid metabolism / 7E-11 - 2E-2 / 26 / 1E-6- 3E-02 / 26
Molecular Transport / 3E-6 - 2E-2 / 55 / 2E-6 - 3E-02 / 26
Top canonical pathways
Mid PFNA + Mix / Mid PFNA
Name / p-value / Ratio / p-value / Ratio
Fatty acid -oxidation I / 1E-11 / 9/29 / 3E-10 / 8/29
Glutaryl-CoA degradation / 7E-10 / 6/11 / 4E-10 / 6/11
Tryptophan Degradation III / 4E-8 / 6/19 / 2E-8 / 6/19
LPS/IL-1 Mediated Inhibition of RXR Function / 2E-6 / 12/203 / 1E-6 / 12/203
Ketogenesis / 3E-6 / 4/10 / 2E-6 / 4/10
Top molecules
Log2 fold change up-/down-regulated
Gene, description / Mid PFNA + Mix / Mid PFNA
Cyp4a11 / 2.568 / 2.419
Cyp2b6 / 2.561 / 2.120
Aldh1a1 / 2.532 / 2.077
Vnn1 / 2.059 / 2.036
Acot1 / 2.052 / 1.278
Colq / 1.954 / 1.514
Aig1 / 1.908 / 1.788
Cd36 / 1.867 / 1.276
Ehhadh / 1.860 / 1.702
Ech1 / 1.856 / 1.512
Fabp5 / -1.508 / -1.392
Erich5 / -1.372 / -0.956
Cxcl13 / -1.173 / -0.817
Ca1 / -1.132 / -0.782
Hsd11b1 / -1.082 / -0.446
Phgdh / -0.976 / -1.271
Mon1b / -0.877 / -0.317
Isyna1 / -0.825 / -0.906
Nfic / -0.631 / -0.262
Top hepatotoxic functions
Mid PFNA + Mix / Mid PFNA
Name / p-value / # molecules / p-value / # molecules
Liver steatosis / 3E-8 - 2E-1 / 16 / 1E-9 - 4E-2 / 17
Hepatocellular peroxisome proliferation / 1E-4 - 7E-2 / 4 / 1E-04 - 7E-2 / 4
Liver necrosis/cell death / 1E-2 - 4E-1 / 2 / 5E-1 / 2
Liver proliferation / 2E-2 - 1E0 / 2 / NA / 0
Liver hypertrophy / 3E-2 / 1 / 3E-2 / 1