Supplementary Table 3. Comparisons of different treatments with standard H2SO4 method; metabolites with no interaction

Metabolite / 0.63M HCl / 1M HCl / 2M HCl / NaOH/BF3 / NaOCH3
1h / NaOCH3 16h / KOH/ H2SO4
n-15:0 FA / + / + / - / + / - / - / +
n-17:0 FA / - / + / - / +
n-18:0 FA / - / -
n-20:0 FA / -
n-21:0 FA / - / - / - / - / - / -
n-22:0 FA / - / - / -
n-24:0 FA / - / - / + / -
n-25:0 FA / - / -
n-26:0 FA / - / - / -
n-28:0 FA / - / - / - / -
n-29:0 FA / - / -
iso-15:0 FA / + / + / - / + / - / - / +
18:1 FA / + / +
n-21:0 ol / + / + / + / + / + / +
n-23:0 ol / + / + / + / + / + / +
n-24:0 ol / + / + / + / +
n-26:0 ol / + / + / + / + / +
n-27:0 ol / + / + / + / + / +
b-Sitosterol / + / + / + / + / - / - / -
D5,24(25)-Stigmastadienol / + / + / + / + / - / - / -
cis-Ferulic acid / + / + / + / - / + / + / -
Solanidine / + / + / + / - / - / - / -

+ = significantly higher than standard H2SO4 mean (P<0.05)

- = significantly lower than standard H2SO4 mean (P<0.05)

Blank = no significant difference from standard H2SO4 mean

Supplementary Table 4A. Comparisons of different treatments with standard H2SO4 method for Desiree; metabolites with interaction

Metabolite / 0.63M HCl / 1M HCl / 2M HCl / NaOH/BF3 / NaOCH3
1h / NaOCH3 16h / KOH/ H2SO4
n-14:0 FA / + / + / + / + / + / +
n-16:0 FA / + / + / - / -
n-30:0 FA / - / - / - / -
iso-17:0 FA / +
15:1 FA / + / + / + / +
16:1 FA / + / +
19:1 FA / - / + / + / +
18:2 FA / + / + / - / - / -
18:3 FA / + / + / - / - / - / -
2-OH 16:0 FA / + / + / + / - / -
2-OH 24:0 FA / + / + / + / - / -
n-22:0 ol / + / + / + / + / +
n-28:0 ol / - / - / + / +
n-29:0 ol
n 30:0 ol
Stigmasterol / + / + / + / -
D5,23-Stigmastadienol / + / + / + / + / - / - / -
Fucosterol / + / + / + / - / - / -
D5-Avenasterol / - / - / - / - / - / - / -
trans-Ferulic acid / + / + / + / - / + / + / -
23:0 alkane / + / + / + / +


Supplementary Table 4B. Comparisons of different treatments with standard H2SO4 method for Phureja; metabolites with interaction

Metabolite / 0.63M HCl / 1M HCl / 2M HCl / NaOH/BF3 / NaOCH3
1h / NaOCH3 16h / KOH/ H2SO4
n-14:0 FA / + / + / + / + / + / +
n-16:0 FA / + / + / - / -
n-30:0 FA / - / -
iso-17:0 FA / + / +
15:1 FA / + / - / + / + / +
16:1 FA / + / + / + / +
19:1 FA / + / + / +
18:2 FA / + / - / - / -
18:3 FA / + / - / -
2-OH 16:0 FA / + / + / + / - / - / -
2-OH 24:0 FA / + / + / + / - / - / -
n-22:0 ol / + / + / + / + / +
n-28:0 ol / + / + / +
n-29:0 ol / + / +
n 30:0 ol / + / + / +
Stigmasterol / + / + / + / + / -
D5,23-Stigmastadienol / + / + / + / + / - / - / -
Fucosterol / + / + / + / + / - / - / -
D5-Avenasterol / - / - / - / - / - / - / -
trans-Ferulic acid / + / + / + / - / * / * / -
23:0 alkane / + / + / + / +

+ = significantly higher than standard H2SO4 mean (P<0.05)

- = significantly lower than standard H2SO4 mean (P<0.05)

Blank = no significant difference from standard H2SO4 mean

* = missing values