Supplementary table 2. Latency (exploratory latency in seconds)

C
O
L
O
R / Training (sec) / F= familiar
N= novel / 1.5 h
Testing (sec) / 24 h
Testing (sec)
Saline / PhB / Nic / Nic+PhB / Saline / PhB / Nic / Nic+PhB
29.57±8.11 / F: red
N: red / 20.21 ± 1.50
18.44 ± 7.02 / 17.54 ± 4.21
14.89 ± 7.99 / 13.24 ± 6.21
15.15 ± 3.58 / 18.80 ± 5.00
21.22 ± 5.87 / 26.21 ± 2.45
22.67 ± 7.00 / 23.55 ± 4.41
30.85 ± 8.00 / 21.45 ± 6.76
19.98 ± 7.77 / 25.00 ± 5.87
26.88 ± 6.85
44.15±9.55 / F: yellow
N: yellow / 31.47 ± 6.87
32.89 ± 5.47 / 29.87 ± 4.02
35.87 ± 6.14 / 31.44 ± 6.75
31.32 ± 5.12 / 26.57 ± 5.87
26.21 ± 6.85 / 32.89 ± 2.54
35.67 ± 3.00 / 43.55 ± 4.40
40.55 ± 4.00 / 34.44 ± 6.80
32.98 ± 3.74 / 35.00 ± 5.78
33.88 ± 3.82
33.57±16.09 / F: red
N: yellow / 30.54 ± 4.40
48.67 ±5.21# / 29.54 ± 4.40
39.56 ± 8.00 / 21.44 ± 6.76
52.00 ±7.56*## / 35.00 ± 5.78
42.74 ± 6.90 / 22.89 ± 2.52
40.67 ±7.00# / 19.55 ± 4.38
39.46 ±8.00# / 23.44 ± 7.46
37.46 ± 7.78 / 13.00 ± 5.84
42.88 ±6.31#
53.57±14.15 / F: yellow
N: red / 44.04 ± 7.40
21.67 ± 6.22*# / 40.55 ± 4.35
23.33 ± 7.01* / 58.44 ± 6.76
21.84 ±7.77*## / 65.00 ± 5.93
24.26 ±6.84*## / 31.89 ± 2.56
19.22 ± 6.04* / 37.55 ± 4.40
17.76 ±6.21*# / 26.28 ± 6.66*
19.33 ±5.77* / 33.00 ± 5.86
20.55 ±6.90*
S
H
A
P
E
+
S
I
Z
E / Training (sec) / F=familiar
N= novel / 1.5 h
Testing (sec) / 24 h
Testing (sec)
Saline / PhB / Nic / Nic+PhB / Saline / PhB / Nic / Nic+PhB
28.55 ± 11.0 / F: red cube
N: red ball / 37.21 ± 1.35
32.45 ± 7.50 / 29.54 ±4.11
30.87 ± 8.01 / 27.24 ± 6.32
25.15 ± 3.58 / 30.80 ± 5.00
38.22 ± 5.79 / 26.21 ± 2.05
22.67 ± 7.00 / 23.45 ± 4.47
30.85 ± 8.00 / 21.41 ± 6.83
19.98 ± 7.79 / 25.15 ± 5.18
27.01 ± 6.55
52.15 ± 9.51 / F: yellow cube
N: yellow ball / 52.47 ± 6.91
62.89 ± 5.29 / 63.87 ± 6.02
45.87 ± 6.14 / 51.44 ± 6.76
55.32 ± 5.12 / 66.57 ± 5.83
56.21 ± 6.75 / 32.89 ± 2.68
55.67 ± 3.00 / 53.51 ± 4.98
40.55 ± 4.00 / 51.34 ± 6.85
62.98 ± 3.82 / 65.00 ± 5.63
53.88 ± 3.37
30.25 ± 8.55 / F: red cube
N: red tower / 18.25 ± 3.87
29.25 ± 5.53 / 24.00 ± 5.35
18.47 ± 4.87 / 25.21 ± 6.58
35.21 ± 6.21 / 28.20 ± 5.31
28.55 ± 5.47 / 22.34 ± 2.55
29.87 ± 5.66 / 22.41 ± 6.21
29.14 ± 7.21 / 38.20 ± 6.87
30.15 ± 7.34 / 19.58 ± 5.85*
33.14 ± 5.54
59.25 ± 8.49 / F:yellow cube
N: yellow tower / 48.25 ± 1.87
57.25 ± 5.65 / 64.00 ± 5.21
58.47 ± 4.90 / 55.21 ± 7.02
54.93 ± 6.84 / 48.25 ± 5.54
58.55 ± 6.05 / 62.34 ± 2.55
51.87 ± 6.03 / 52.42 ± 6.13
59.45 ± 7.54 / 58.21 ± 6.87
70.15 ± 7.37 / 49.55 ± 5.86
53.14 ± 5.54

Latency (in seconds)

C
O
L
O
R
+
S
I
Z
E
+
S
H
A
P
E / Training
(sec) / F= familiar
N= novel / 1.5 h
Testing (sec) / 24 h
Testing (sec)
Saline / PhB / Nic / Nic+PhB / Saline / PhB / Nic / Nic+PhB
48.78 ±
7.65 / F: red cube
N: yellow ball / 32.01 ± 4.40
53.67±7.00*# / 31.55 ± 4.53
55.76 ±8.00*# / 17.44 ± 6.73
61.98±7.76*## / 24.22 ± 5.46
49.22 ±6.78*# / 32.89 ± 2.55
59.67 ±7.00*# / 35.55 ± 4.40
53.76±8.00*# / 39.44 ± 6.72
51.98±7.77*# / 27.00 ± 5.85
44.88 ±5.88*#
31.78 ±
5.11 / F: red cube
N:yellow tower / 22.70 ± 1.40
42.19 ±4.55*# / 23.55 ± 4.50
37.78 ±5.22*# / 18.44 ± 3.80
39.22 ±6.58*# / 25.00 ± 5.77
32.85 ± 6.88 / 27.01 ± 4.55
38.45 ±7.05 / 18.55 ± 4.39
41.76 ±8.11*# / 21.44 ± 6.75
26.98 ± 7.65 / 25.00 ± 5.97
36.88 ± 6.88
51.57 ±
14.12 / F: yellow cube
N: red ball / 41.04 ± 7.40
29.67 ± 6.21 / 34.51 ± 4.38
20.33 ±7.01* / 41.44 ± 6.75
18.84 ±7.76#* / 35.00 ± 5.87
14.26±6.86#** / 29.89 ± 2.55
21.22 ±6.04* / 37.55 ± 4.40
19.76 ±6.21*# / 29.28 ± 6.80
16.33 ± 5.77* / 31.00 ± 5.76
22.55 ±6.87*
47.57 ±
13.95 / F: yellow cube
N: red tower / 30.89 ± 4.55
19.22 ±6.04* / 34.55 ± 4.40
20.76 ±6.21*# / 26.28 ± 6.70
19.33±5.72* / 33.00 ± 5.85
20.54 ±6.87* / 34.04 ± 7.41
21.66 ±6.21* / 39.55 ± 4.42
20.33 ±7.01*# / 38.44 ± 6.76
23.84 ±7.76* / 35.00 ± 5.85
24.25 ±6.87*

Zebrafish were trained individually and then immersed for 10 min in tanks containing: drug-free tank water (saline), 15 mg/l nicotine (Nic), 15 mM phenylbutyrate (PhB) or 15 mg/l nicotine plus 15 mM PhB (Nic+PhB). Drugs were dissolved in fish-tank water. Data are presented as mean ± SEM; n = 1014 animals per group. Differences in the latency were assessed using ANOVA and Scheffé test. Asterisks compare values in test and training sessions, * p < 0.05; numeral symbols compare familiar and novel object data in test sessions, # p < 0.05, ## p < 0.01.