Supplementary Table 2. Correlation of PTEN FISH with clinico-pathological parameters in Breast Cancer

Total / PTEN Deleted / PTEN Normal / Gain / p value
N / % / N / % / N / %
Total Number of Cases / 934 / 60 / 6.4 / 874 / 93.6
Age
< 50 / 642 / 68.7 / 39 / 6.1 / 603 / 93.9 / 0.5226
> 50 / 292 / 31.3 / 21 / 7.2 / 271 / 92.8
Tumor size*
≤ 2 cm / 206 / 22.5 / 16 / 7.8 / 190 / 92.2 / 0.4314
>2 cm / 710 / 77.5 / 44 / 6.2 / 666 / 93.8
Lymph Nodes*
Negative / 288 / 32.6 / 21 / 7.3 / 267 / 92.7 / 0.3724
Positive / 594 / 67.4 / 34 / 5.7 / 560 / 94.3
Metastasis*
M0 / 760 / 90.6 / 40 / 5.3 / 720 / 94.7 / 0.4087
M1 / 79 / 9.4 / 6 / 7.6 / 73 / 92.4
Tumour Stage*
I / 74 / 9.1 / 5 / 6.7 / 69 / 93.3 / 0.5988
II / 362 / 44.4 / 22 / 6.1 / 340 / 93.9
III / 301 / 36.9 / 13 / 4.3 / 288 / 95.7
IV / 79 / 9.7 / 6 / 7.6 / 73 / 92.4
Histological Grade *
Well differentiated / 73 / 7.9 / 3 / 4.1 / 70 / 95.9 / 0.6585
Moderately differentiated / 466 / 50.4 / 31 / 6.6 / 435 / 93.4
Poorly differentiated / 386 / 41.7 / 26 / 6.7 / 360 / 93.3
Histology*
Infiltrating Ductal Carcinoma / 852 / 94.0 / 58 / 6.8 / 794 / 93.2 / 0.0250
Infiltrating Lobular / 40 / 4.4 / 0 / 0 / 40 / 100.0
Mucinous Ca / 14 / 1.6 / 0 / 0 / 14 / 100.0
LVI*
Yes / 349 / 58.4 / 18 / 5.2 / 331 / 94.8 / 0.6354
No / 349 / 41.6 / 29 / 5.9 / 461 / 94.1
ETE*
Yes / 259 / 33.5 / 16 / 6.2 / 343 / 93.8 / 0.6765
No / 515 / 66.5 / 28 / 5.4 / 487 / 94.6
Recurrence*
Yes / 240 / 29.0 / 12 / 5.0 / 228 / 95.0 / 0.5866
No / 588 / 71.0 / 35 / 6.0 / 553 / 94.0
ER*
Negative / 325 / 34.8 / 30 / 9.2 / 295 / 90.8 / 0.0127
Positive / 608 / 65.2 / 30 / 4.9 / 578 / 95.1
PR*
Negative / 396 / 42.7 / 34 / 8.6 / 362 / 91.4 / 0.0244
Positive / 532 / 57.3 / 26 / 4.9 / 506 / 95.1
HER-2 IHC*
Negative / 668 / 71.7 / 39 / 5.8 / 629 / 94.2 / 0.2448
Positive / 264 / 28..3 / 21 / 7.9 / 243 / 92.1
Triple Negative*
Yes / 140 / 15.1 / 17 / 12.1 / 123 / 87.9 / 0.0065
No / 787 / 84.9 / 43 / 5.5 / 744 / 94.5
Ki-67*
Positive / 588 / 64.3 / 39 / 6.6 / 549 / 93.4 / 0.9110
Negative / 326 / 35.7 / 21 / 6.4 / 305 / 93.6
pAKT (ser-473)*
Negative / 697 / 77.9 / 40 / 5.7 / 657 / 94.3 / 0.0642
Positive / 198 / 22.1 / 19 / 9.6 / 179 / 90.4
PTEN IHC*
High / 206 / 23.1 / 9 / 4.4 / 197 / 95.6 / 0.1049
Low / 684 / 76.9 / 51 / 7.5 / 633 / 92.5
Basal-like Breast Cancer
Yes / 126 / 13.5 / 14 / 11.1 / 112 / 88.9 / 0.0324
No / 808 / 86.5 / 46 / 5.7 / 762 / 94.3
Survival
OS 5 Years / 77.4 / 80.8 / 0.7978

* Data was not available (NA) for some cases: Tumor size (NA=18), Lymph nodes (NA=52), Metastasis (NA=95), Stage (NA=118), Grade (NA=09) , Histology (NA=28), LVI(NA=236), ETE (NA=160), Recurrence (NA=106) , ER (NA=01), PR (NA=02), Triple Negative(NA=07), Ki-67 (NA=20), pAKT(ser-473) (NA=39) & PTEN IHC (NA=44).