Supplementary Table 1Substrate Utilization pattern of the thermotolerant isolates

Substrates / M4 / M5 / M6 / M7 / M8 / M43 / M46 / M47 / M48 / M49 / M50 / M55 / M70
Saccharides
α-D-Glucose / + / + / + / + / + / + / + / + / - / + / + / + / +
D – Mannose / + / - / + / - / + / + / + / + / - / + / + / - / +
D – Fructose / + / + / + / + / + / + / + / + / + / + / + / - / +
D – Galactose / - / - / - / - / + / + / + / + / - / + / + / - / +
3 – Methyl Glucose / - / - / - / - / - / + / - / - / - / - / - / - / -
D – Fucose / - / - / - / - / - / + / + / - / - / + / + / - / -
L – Fucose / - / - / - / - / + / + / + / - / + / + / + / - / +
L – Rhamnose / - / - / - / - / + / + / - / - / - / - / + / - / -
D – Maltose / + / - / - / - / + / + / - / + / - / - / - / - / +
D – Trehalose / + / - / + / - / + / + / - / + / - / + / - / - / +
D –Cellobiose / + / + / + / + / + / + / - / + / - / - / - / - / +
Gentiobiose / + / - / - / - / + / + / - / + / - / - / - / - / +
Sucrose / + / + / + / + / + / + / - / + / + / - / - / + / +
D – Turanose / + / - / - / - / + / + / - / + / - / - / - / - / +
α - D - Lactose / - / - / - / - / - / + / - / - / - / - / - / - / +
D – Melibiose / - / - / - / - / + / + / - / + / - / - / - / - / +
D-Raffinose / - / - / - / - / + / - / - / + / - / - / - / - / +
Stachyose / - / - / - / + / + / + / - / - / - / - / - / - / -
Dextrin / + / - / - / - / + / + / - / + / + / - / - / - / +
Pectin / - / + / - / + / + / + / - / + / - / - / - / - / +
Saccharide derivatives
D – Sorbitol / + / - / + / - / + / + / - / - / + / - / - / - / +
D - Mannitol / + / - / + / - / + / + / - / + / + / - / - / - / +
D – Arabitol / - / - / - / - / - / + / - / - / + / - / - / - / +
myo - Inositol / + / - / + / - / + / + / - / + / + / - / - / - / +
Glycerol / + / - / + / - / + / + / + / + / + / + / - / - / +
β– Methyl – D - Glucoside / + / - / + / - / + / + / - / + / + / - / - / - / +
D – Salicin / + / + / + / + / + / + / - / + / + / - / - / - / +
D -Galacturonic acid / + / - / + / - / + / + / + / - / + / - / + / - / +
L – Galactonic acid / + / + / + / + / + / + / + / - / + / + / + / - / +
D – Gluconic acid / + / + / + / + / + / + / + / + / + / + / - / - / +
D – Glucuronic acid / + / - / - / - / + / + / + / - / + / + / + / - / +
Substrates / M4 / M5 / M6 / M7 / M8 / M43 / M46 / M47 / M48 / M49 / M50 / M55 / M70
Glucuronamide / + / + / + / + / + / + / + / + / - / + / + / + / +
Mucic acid / + / - / + / - / + / + / - / - / + / - / + / - / -
Quinic acid / - / + / - / + / - / + / + / + / + / + / + / - / -
D – Saccharic acid / + / - / + / - / - / + / - / + / + / - / - / - / -
N- Acetyl-D - Glucosamine / + / - / - / - / + / + / - / + / + / - / - / - / +
N – Acetyl – β - D - Mannosamine / - / - / - / - / + / - / - / + / + / - / - / - / +
N – Acetyl – D - Galactosamine / - / - / - / - / - / + / - / + / - / - / - / - / -
N – Acetyl Neuraminic acid / - / - / - / - / - / + / - / + / + / - / - / - / +
Metabolic intermediates
D -Glucose - 6 -PO4 / - / - / - / - / + / + / - / + / + / - / - / - / -
D -Fructose - 6 – PO4 / + / + / + / + / + / + / + / + / + / + / + / + / +
p - Hydroxy -Phenylacetic acid / - / - / - / - / - / - / - / + / - / - / + / - / -
Methyl Pyruvate / - / - / - / - / - / + / + / - / - / - / - / - / +
D – Lactic acid Methyl Ester / - / - / - / - / + / + / - / + / + / - / - / - / +
L – Lactic acid / + / - / - / - / + / + / + / + / + / + / - / - / +
Citric acid / - / + / + / + / + / + / + / - / + / + / + / - / +
α - keto- Glutaric acid / - / + / - / + / + / - / + / - / + / + / - / - / +
D -Malic acid / - / + / - / + / - / - / + / - / - / + / - / - / -
L - Malic acid / + / + / + / + / + / + / + / + / + / + / - / - / +
α -Hydroxy -Butyric acid / - / - / - / - / - / - / - / - / + / - / - / - / -
β-Hydroxy - Butyric acid / - / - / - / - / - / + / - / + / + / - / - / - / -
α - keto- Butyric acid / - / - / - / - / - / - / - / - / + / + / - / - / -
Acetoacetic acid / - / - / - / - / + / - / - / + / + / + / + / - / +
Propionic acid / - / - / - / - / + / - / - / - / + / - / - / - / -
Acetic acid / - / - / - / - / + / + / - / + / + / - / + / - / +
Formic acid / - / - / - / - / + / - / - / - / - / - / - / + / +
γ – Amino Butyric acid / - / + / - / + / - / - / + / - / + / + / + / - / +
Bromosuccinic acid / - / + / - / + / + / + / - / - / + / - / - / - / +
Amino acids and peptides
D -Aspartic acid / - / + / - / + / + / + / - / - / + / - / - / - / -
D - Serine / - / - / - / - / - / + / - / - / + / - / + / - / -
L-Alanine / + / + / + / + / + / + / - / + / + / - / - / - / +
L - Arginine / - / + / - / + / + / + / - / + / + / - / - / - / +
Substrates / M4 / M5 / M6 / M7 / M8 / M43 / M46 / M47 / M48 / M49 / M50 / M55 / M70
L -Aspartic acid / - / + / - / + / + / + / - / - / + / - / - / - / +
L – Glutamic acid / - / + / - / + / + / + / + / + / + / + / - / - / +
L - Histidine / - / - / - / - / - / + / - / - / + / - / - / - / +
L – Pyroglutamic acid / - / - / - / - / + / + / - / - / + / - / - / - / -
L - Serine / - / + / - / + / + / + / - / + / - / - / + / - / +
Inosine / - / - / - / - / + / + / - / + / - / - / - / - / -
Glycyl -L - Proline / - / - / - / - / + / + / - / + / + / - / - / - / +
Gelatin / - / + / - / + / + / + / - / + / + / - / - / - / -

Supplementary Table 2Resistance against chemical agents and Growth conditions

Antimicrobial compounds/Growth conditions / M4 / M5 / M6 / M7 / M8 / M43 / M46 / M47 / M48 / M49 / M50 / M55 / M70
Antimicrobial compounds
Troleandomycin / - / - / - / - / - / + / + / - / - / + / + / - / -
Rifamycin / + / - / - / - / - / + / + / - / - / + / + / - / -
Minocycline / - / - / - / - / - / - / - / - / - / - / + / - / -
Fusidic acid / - / - / - / - / - / + / + / - / - / + / + / - / -
Lincomycin / + / - / - / - / - / + / + / - / - / + / + / - / -
Vancomycin / - / - / - / - / - / + / + / - / - / + / + / - / -
Nalidixic acid / - / - / - / - / - / - / + / + / + / + / + / - / +
Aztreonam / + / + / + / + / + / + / + / + / + / + / + / - / +
Guanidine HCL / + / + / - / + / + / + / + / + / + / + / + / + / +
Niaproofu / - / - / - / - / - / + / + / - / - / + / + / - / -
Potassium Tellurite / + / + / + / + / + / + / - / + / + / - / + / + / +
Sodium Butyrate / + / + / - / + / + / + / - / + / - / - / - / + / +
Sodium Bromate / - / - / - / - / - / - / - / + / - / - / - / - / -
1% Sodium Lactate / + / + / + / + / + / + / + / + / + / + / + / + / +
Lithium Chloride / + / - / + / - / + / + / + / + / - / + / + / + / +
Tetrazolium Violet / - / - / - / - / - / + / + / - / - / + / + / - / -
Tetrazolium Blue / - / - / - / - / - / + / + / - / - / + / + / - / -
Growth conditions
pH6 / + / + / + / + / + / + / + / + / + / + / + / + / +
pH5 / + / + / + / + / + / + / + / - / - / + / + / - / +
1% NaCl / + / + / + / + / + / + / + / + / + / + / + / + / +
4% NaCl / + / + / + / + / + / + / + / + / - / + / + / + / +
8% NaCl / + / + / + / + / + / - / - / + / - / + / - / + / +