Supplementary Table 1. Sequence of the Oligonucleotides and Restriction Enzymes Used For

Supplementary Table 1. Sequence of the oligonucleotides and restriction enzymes used for plasmid construction

DNA constructs / Sequence (5’ 3’) / Restriction
Enzyme site / Amplicon (bp)
pFlag-p110 / F: CCAAAGCTTCCTCCACGACCATCT
R: ATCTCTAGATCAGTTCAAAGCATGCTG / HindIII
XbaI / 3207
pFlag-NBS / F: CCCAAGCTTATGTGGAAACTGCTGC
R: CGGGATCCATCCTCAGTTATCTTCTC / HindIII
BamHI / 2262
pcDNA3-NBS1-653 / F: CCCAAGCTTATGTGGAAACTGCTGC
R: CGGGATCCTTACTTTTTTGGAAGCATC / HindIII
BamHI / 1959
pcDNA3-NBS221-754 / F: TAGCGGATCCAGCAGCCTCCAC
R: TTGGCCTGAAGCGGCCGCTTACTAGGAA / BamHI
NotI / 1602
pcDNA3-NBS1-704 / F: CCCAAGCTTATGTGGAAACTGCTGC
R: CGGGATCCTTATCCAATGATGTGTGG / HindIII
BamHI / 2112
pFlag-p110 / F: CCAAAGCTTCCTCCACGACCATCT
R: ATCTCTAGATTACCCACAGGCACGGCA / HindIII
XbaI / 2088
pFlag-p110 / F: CCAAAGCTTCCTCCACGACCATCT
R: ATCTCTAGATTATCTTAACTCATTGTC / HindIII
XbaI / 1572
pFlag-p110 / F: AGTAATGCGGCCGCGACATCTACGAAATCC
R: ATCTCTAGATCAGTTCAAAGCATGCTG / NotI
XbaI / 2241
pFlag-p110 / F: CCAAAGCTTCCTCCACGACCATCT
R: ATCTCTAGATTATCCATTCATGTAGGG / HindIII
XbaI / 963
pFlag-p110 / F: CCAAAGCTTCCTCCACGACCATCT
R: TGAGGATTCTAGATTAACGGTTGCCTACTG / HindIII
BamHI / 324
pCITE-4a-p1101-25 / F: CCGGAATTCATGCCTCCACGACC
R: CCGCTCGAGTCATAAACATTCTACTAGG / EcoRI
XhoI / 75
pCITE-4a-p110 / F: CCGGAATTCATGCCTCCACGACC
R: CCGCTCGAGCTTTTCAAAATAGTTCATGC / EcoRI
XhoI / 150
pCITE-4a-p1101-75 / F: CCGGAATTCATGCCTCCACGACC
R: CCGCTCGAGTCATTGAGTAACACTTACG / EcoRI
XhoI / 225
pCITE-4a- p11 / F: TAACCTACGAATTCATGCCTCCACGACC
R: TGAGGATCTTCTCGAGACGGTTGCC / EcoRI
XhoI / 324
pFlag-NBS1-704 / F: CCCAAGCTTATGTGGAAACTGCTGC
R:TCCCCCGGGTTATCCAATGATGTGTG / HindIII
SmaI / 2112
pFlag-NBS1-687 / F: CCCAAGCTTATGTGGAAACTGCTGC
R: GCTCTAGATTATTTCTTGAAATTTTTTAG / HindIII
XbaI / 2061
pFlag-NBS1-669 / F: CCCAAGCTTATGTGGAAACTGCTGC
R: GCTCTAGATTAGGAAGTAGAGTTTTTAAAC / HindIII
XbaI / 2007
pFlag-NBS1-653 / F: CCCAAGCTTATGTGGAAACTGCTGC
R: CGGGATCCTTACTTTTTTGGAAGCATC / HindIII
BamHI / 1959
pGEX-NBS402-653 / F: CGAGGATCCGTAAAGGAGTCCTGC
R: ATCCTCGAGTTATTTTGGAAGCATCTC / BamHI
XhoI / 756
pGEX-NBS653-754 / F: CGAGGATCCAAGCTGTTATTGACT
R: ATCCTCGAGTTATCAGTTATCTTCTCC / BamHI
XhoI / 306
pGEX-NBS653-669 / F: GTGAGATGCTTGAATTCAAGCTGTTATTG
R: GCCAGATGGCTCGAGGGAAGTAGAG / EcoRI
XhoI / 51
pGEX-NBS670-686 / F: CCGGAATTCAGAAATCCATCTGGC
R: CCGCTCGAGCTTGAAATTTTTTAGTTG / EcoRI
XhoI / 51
pGEX-NBS687-704 / F: CCGGAATTCAAATTCAAAAAGGTCAC
R: CCGCTCGAGTCCAATGATGTGTGG / EcoRI
XhoI / 54
pET32a-NBS402-653 / F: CAAGATGAATTCACTGTAAAGGAGTCCTG
R: CAGTCAACTCGAGCTTTTTTGGAAGC / EcoRI
XhoI / 756
pET32a-NBS653-669 / F: GTGAGATGCTTGAATTCAAGCTGTTATTG
R: GCCAGATGCTCGAGGGAAGTAGAG / EcoRI
XhoI / 51
pET32a-NBS653-754 / F: GTGAGATGCTTGAATTCAAGCTGTTATTG
R: CTTCTTTTTAAAATCCTCTCGAGTCTTCTCC / EcoRI
XhoI / 306
pHA-p110 / F: CCGGAATTCCGATGTGCTTCAGTTTCATAATG
R: CCGCTCGAGGTTAAGATCTGTAGTCTTTCCG / EcoRI
XhoI / 3213
pHA-p110 / F: GTAACGAATTCGGATGCCCCCTGGG
R: CTGGGAGCTCGAGCTACTGCCTGTTGTC / EcoRI
XhoI / 3135
pHA-p110 / F:ACGCGTCGACCATGGAGCTGGAGAAC
R:CCGCTCGAGTTAGGCTGAATGTTTCTC / SalI
XhoI / 3309
pET32a-NBS653-669
(AAAATEFRSLV) / F:AATTCGCGGCGGCAGCGACTGAATTTAGATCACTGGTGATTAAAAACTCTACTTCCC
R:TCGAGGGAAGTAGAGTTTTTAATCACCAGTGATCTAAATTCAGTCGCTGCCGCCGCG / EcoRI
XhoI / 51
pET32a-NBS653-669
(KLLLAAAASLV) / F:AATTCAAGCTGTTATTGGCTGCAGCTGCATCACTGGTGATTAAAAACTCTACTTCCC
R:TCGAGGGAAGTAGAGTTTTTAATCACCAGTGATGCAGCTGCAGCCAATAACAGCTTG / EcoRI
XhoI / 51
pET32a-NBS653-669
(KLLLTEFRAAA) / F:AATTCAAGCTGTTATTGACTGAATTTAGAGCAGCGGCGATTAAAAACTCTACTTCCC
R:TCGAGGGAAGTAGAGTTTTTAATCGCCGCTGCTCTAAATTCAGTCAATAACAGCTTG / EcoRI
XhoI / 51
pcDNA3-NBS1
(AAAATEFR) / F1: GCGGATCCATGTGGAAACTGCTG
R1: ATCTAAATTCAGTCGCTGCCGCCGCTTTT GGAAGCATC
F2: CACTGGTGATTAAAAACTC
R2: CCGCTCGAGTTATCTTCTCCTTTTTAAATA AG / BamHI
EcoRV
EcoRV
XhoI / 2262
pcDNA3-NBS1
(KLLLAAAA) / F1: GCGGATCCATGTGGAAACTGCTG
R1:ATCACCAGTGATGCAGCAGCAGCCAATAACAGCTTTTTTGGAAG
F2: TAAAAACTCTACTTCCAG
R2: CCGCTCGAGTTATCTTCTCCTTTTTAAATA AG / BamHI
EcoRV
EcoRV
XhoI / 2262
pcDNA3-NBS1
(TEFRAAA) / F1: GCGGATCCATGTGGAAACTGCTG
R1: ATCGCCGCTGCTCTAAATTCAGTCAATAA CAG
F2: TAAAAACTCTACTTCCAG
R2: CCGCTCGAGTTATCTTCTCCTTTTTAAATA AG / BamHI
EcoRV
EcoRV
XhoI / 2262