Supplementary Table 1. Primers Used to Obtain the Sequence of BNRBV-RL

Supplementary Fig. 1. Alignment of 5’ and 3’UTRs of BNRBV-RL and BNRBV showing blocks of conserved nucleotides as indicated by shadowing. Arrows and numbers at the end of the 5’UTR alignment indicate the length of the UTR. Blocks at the 3’ UTR are numbered BI-BIII. Numbers at the beginning and between blocks in the 3’ UTRs indicate the number of nucleotides not shown. The nucleotides between the blocks shared a minimum of 70% nucleotide sequence identity between the equivalent regions in BNRBV-RL and BNRBV.

Supplementary Table 1. Primers used to obtain the sequence of BNRBV-RL

Amplicon / Primers (forward, reverse) / Amplicon size (bp) / RNA segment
A / 5´RACE
JAP932 GTTGTCTGAATCCAACATGGC / 694 / RNA1
B / JAP903 ATCACTACAAAATGACTACAGCC
JAP906 AGAACCATCTCCTTCGAACC / 2391 / RNA1
C / JAP918 TGGGAAAAATTTAAGGTGTTGGG
JAP919 CTCAAAAATTGGGATTGCAGCG / 1565 / RNA1
D / JAP904 GAACTAATGTCTCTTGATGGG
JAP906 AGAACCATCTCCTTCGAACC / 350 / RNA1
E / JAP920 TTTGTTGTCGATGACGACGCG
JAP921 GTCTTTCCACAACCTGGACCG / 1221 / RNA1
F / JAP904 GAACTAATGTCTCTTGATGGG
JAP905 TAGGGATAACTGAAAACCCGG / 3277 / RNA1
G / JAP933 TGCTCAACAAAAGACTCACGC
3´RACE / 404 / RNA1
H / 5´RACE
JAP934 CACGAGAATGCATCTTCAGGG / 710 / RNA2
I / JAP916 AGAGTCGAAAGAGCTTATGGG
JAP917 GACACGGTGAATACAACACCG / 2031 / RNA2
J / JAP922 GTATCGTATTTTCCGAGTCTGCG
JAP923 GAAGCAGTTTGGCATTCTTACCG / 608 / RNA2
K / JAP900 TATTGAGCGTGCTGATGTGCG
JAP901 AGTCTAGGTCTGGATGGATCG / 1829 / RNA2
L / JAP935 GTCCACTCATTTCGCACTATTCG
3´RACE / 916 / RNA2
M / 5´RACE
P24-2 GCAGAGGCACCGAGATAGAC / 1679 / RNA3
N / JAP897 ATGCAAGCAACATTGTGCGCC
JAP898 ACTACTTTACTAGTCTACACG / 1326 / RNA3
O / JAP897 ATGCAAGCAACATTGTGCGCC
3´RACE / 1435 / RNA3
P / 5´RACE
P31-1-r GCGTTTCAGCACCACTAAC / 840 / RNA4
Q / P31-1-f CCAGTTTGGAGGAATTGCAT
P31-1-r GCGTTTCAGCACCACTAAC / 432 / RNA4
R / JAP911 CTACTTCTTCACTACCATGCG
JAP912 AACTAAACGGTACGACACCC / 1590 / RNA4
S / P31-1f CCAGTTTGGAGGAATTGCAT
3´RACE / 1315 / RNA4

Supplementary Table 2. Comparison of nucleotide and amino

acid sequence identity of the variant BNRBV-RL to BNRBV

Sequence / % Nucleotide identity / % Amino acid similarity
RNA 1 / 89.0 / ---
Methyltransferase-helicase / 89.1 / 94.1
RNA 2 / 90.5 / --
RNA polymerase / 90.3 / 96.4
RNA 3 / 90.0 / ---
Hypothetical protein 1 / 91.1 / 84.7
p24 / 89.9 / 95.5
Hypothetical protein 2 / 91.6 / 92.1
Hypothetical protein 3 / 86.5 / 86.3
RNA 4 / 86.5 / ---
Movement protein / 93.5 / 97.7