SUPPLEMENTARY TABLE 1: Primers Used for Sequencing of Gyra, Parc, and Pare and The

SUPPLEMENTARY TABLE 1: Primers used for sequencing of gyrA, parC, and parE and the

QRDRs of gyrA and parC

Primer / Sequence (5’-3’) / Nucleotide Position / Source
Amplification Primers
GyrA forward / TAAAAAACTTTGTCACGAATATGCC / 130-105 bp upstream of start / (13)
GyrA reverse / GAGACATTATGCTTCACCTTCTG / 2453-2475 / This study
ParC forward / AAACCTACTCTACATTCTTTGAAAGGAG / 134-106bp upstream of start / (13)
ParC reverse / CCACTCCTTATTCTAAAAACC / 2579-2599 / This study
ParE forward / CCAGATGGAATCGAACCC / 191-175 bp upstream of start / This study
ParE reverse / CCTTTCAAAGAATGTAGAGTAGG / 2234-2256 / This study
Sequencing Primers
GyrA SF1 / CGTTTTAGTGGTTTAGAGGC / 85-66 bp upstream of start / (13)
GyrA SF2 / GGTAAATACCACCCACACG / 217-236 / This study
GyrA SF3 / GATGCAGTGAAGTTGGTC / 567-585 / This study
GyrA SF4 / GGGTATCACAGCAGTACG / 852-868 / This study
GyrA SF5 / CGCTAGACCATATCGACG / 1148-1165 / This study
GyrA SF6 / GCCGTACAGAGTTGATGG / 1439-1460 / This study
GyrA SF7 / GCTACCAGTAGTCAATCTC / 1740-1758 / This study
GyrA SF8 / CGTATCGCCACTGGTGTG / 2041-2058 / This study
GyrA SR1 / GCCACCGCAACCGTTG / 2420-2405 / This study
GyrA SR2 / CACACCAGTGGCGATACG / 2041-2058 / This study
GyrA SR3 / GAGATTGACTACTGGTAGC / 1740-1758 / This study
GyrA SR4 / CCATCAACTCTGTACGGC / 1439-1460 / This study
GyrA SR5 / CGTCGATATGGTCTAGCG / 1148-1165 / This study
GyrA SR6 / CGTACTGCTGTGATACCC / 852-868 / This study
GyrA SR7 / GACCAACTTCACTGCATC / 567-585 / (13)
ParC SF1 / CGCCCTAGATACTGTGTGA / 98-80 bp upstream of start / (13)
ParC SF2 / CTGAGGCACGTTTGTCTG / 356-373 / This study
ParC SF3 / CGTGATGAAATCAAGAAAGC / 664-683 / This study
ParC SF4 / GCTAATACTGAGCTTGTTC / 916-934 / This study
ParC SF5 / GGAAAACCTCAAAGTTAGC / 1206-1224 / This study
ParC SF6 / GGCAGGTTACATCAAGCG / 1524-1541 / This study
ParC SF7 / CGACAACCTACTTTGCAG / 1805-1822 / This study
ParC SF8 / GTCCTTCTACCTCTTGACC / 2109-2127 / This study
ParC SR1 / CCAGCTCTTAGAACTTATTC / 2502-2483 / This study
ParC SR2 / GGTCAAGAGGTAGAAGGAC / 2109-2127 / This study
ParC SR3 / CTGCAAAGTAGGTTGTCG / 1805-1822 / This study
ParC SR4 / CGCTTGATGTAACCTGCC / 1524-1541 / This study
ParC SR5 / GCTAACTTTGAGGTTTTCC / 1206-1224 / This study
ParC SR6 / GAACAAGCTCAGTATTAGC / 916-934 / This study
ParC SR7 / GCTTTCTTGATTTCATCACG / 664-683 / This study
ParC SR8 / AAATCCCAGTCGAACCAT / 493-510 / (13)
ParE SF1 / CTGCTGAAATTGTCACATC / 74-92 bp upstream of start / This study
ParE SF2 / GACGGTAGTCTAACGGTTC / 211-229 / This study
ParE SF3 / CACCAAAGTTACTTTTATGC / 513-532 / This study
ParE SF4 / CAGATAACATTCTATCCTTTG / 803-823 / This study
ParE SF5 / GGAATTAGCTTCTAACCTC / 1116-1132 / This study
ParE SF6 / CCAAGATGGCGGATATCC / 1394-1411 / This study
ParE SF7 / GGTAAAGGCGCTACCCTC / 1714-1731 / This study
ParE SR1 / CTGTCGCTTCTTCTAGCG / 1919-1936 / This study
ParE SR2 / GAGGGTAGCGCCTTTACC / 1714-1731 / This study
ParE SR3 / GGATATCCGCCATCTTGG / 1394-1411 / This study
ParE SR4 / GAGGTTAGAAGCTAATTCC / 1116-1132 / This study
ParE SR5 / CAAAGGATAGAATGTTATCTG / 803-823 / This study
ParE SR6 / GCATAAAAGTAACTTTGGTG / 513-532 / This study
ParE SR7 / GAACCGTTAGACTACCGTC / 211-229 / This study

bp, base pair