Supplementary Table 1 Percentage Distribution of 16S Rrna Gene Clone Sequences in The

Supplementary Table 1 Percentage distribution of 16S rRNA gene clone sequences in the different Coelostomidiidae host speciesclone libraries. Banding patterns based on restriction enzyme digests (using Rsa I and/or Hae III) were used for identification followed by confirmation using sequence identity for each banding pattern. Multiple clone libraries for each host species were made (except U. assimile).

Scale insect species / Site / Clone library / Symbiont types
ID / # of clones / B-symbiont / S-symbiont / E-symbiont / Wolbachia / other bacteria
C. wairoensis / Huia / CW1 / 181 / 42.8 / - / - / 57.2 / -
Leigh / CW2 / 30 / 40.0 / - / 43.3 / 16.7 / -
Waharau / CW3 / 51 / 60.8 / - / 23.5 / 15.7 / -
Wenderholm / CW4 / 63 / 44.4 / - / 39.7 / 15.9 / -
Totals / 325 / 45.6 / - / 36.6 / 35.5 / -
C. montana / Mt. Arthur / CM1 / 68 / 60.9 / - / - / 23.4 / 15.6
Mt. Arthur / CM2 / 65 / 75.4 / - / - / 17.5 / 7.0
Totals / 133 / 68.2 / - / - / 20.5 / 11.3
C. zealandica / Saddle Island / CZ1 / 45 / 95.6 / - / - / 4.4 / -
Saddle Island / CZ2 / 44 / 84.1 / - / - / 15.9 / -
Totals / 89 / 89.8 / - / - / 10.2 / -
C. jenniferae / Bealey Valley / CJ1 / 66 / 13.6 / 62.1 / 24.2 / - / -
Lake Hochstettor / CJ2 / 64 / 3.1 / 60.9 / 35.9 / - / -
Totals / 130 / 8.4 / 61.5 / 30.1 / - / -
C. pilosa / Sharplin Falls / CP1 / 95 / - / 68.4 / 24.2 / 7.4 / -
Takaka Hill / CP2 / 68 / - / 32.4 / 42.6 / 25.0 / -
Totals / 163 / - / 50.4 / 33.4 / 16.2 / -
C. deboerae / Browns Hill / CD1 / 44 / 61.4 / - / - / 38.6 / -
Browns Hill / CD2 / 35 / 80.0 / - / - / 20.0 / -
Totals / 79 / 70.7 / - / - / 29.3 / -
U. brittini / Eve's Valley / UB1 / 67 / 100.0 / - / - / - / -
Mt. Richardson / UB2 / 84 / 98.8 / - / - / - / 1.2
Totals / 151 / 99.4 / - / - / - / 0.6
U. assimile / Takaka Hill / UA1 / 82 / - / 98.8 / - / - / 1.2
U.dracophylli / Bealey Valley / UD1 / 38 / 100.0 / - / - / - / -
Bealey Valley / UD2 / 31 / 100.0 / - / - / - / -
Totals / 69 / 100.0 / - / - / - / -

Supplementary Table 2 List of sequence accession numbers from this study and published data used in the analysis. Coelostomidiidae host (28S rRNA gene and COI gene) and symbiont (16S rRNA gene) sequences are provided in the top half of the table. The sequences marked with a “#” are from Dhami et al. 2012. Sequences from other insect families downloaded from GenBank are provided in the bottom half of the table. For these sequences, the key to the labelsin Figure 3 are also provided.

Sequences from Coelostomidiidae / GenBank sequence accession numbers
Organism / 28S rRNA / COI / Associated symbionts / 16S rRNA
Coelostomidiawairoensis / JX513961 / JX513992 / Cand. Hoataupuhia coelostomidicola (HC) / JQ322763 #
JX513962 / JQ322767 #
JX513963 / JQ322758 #
JX513964 / JQ322766 #
JX513965 / FJ798088 #
JX513976
JX513977 / JQ322761 #
KC447425
JX513978 / KC447426
JX513985 / Wolbachia / JQ322764 #
JQ322772 #
JQ322759 #
FJ798089 #
JQ322757 #
KC447449
E-symbiont / JQ322769 #
JQ322771 #
JQ322762 #
JQ322760 #
JQ322768 #
JQ322770 #
JQ322765 #
Coelostomidiajenniferae / JX513966 / JX513987 / E-symbiont / KC447385
JX513975 / JX513988 / KC447386
HC / KC447417
S-symbiont / KC447391
KC447392
KC447393
KC447394
KC447395
KC447396
Coelostomidiamontana / JX513968 / JX513989 / HC / KC447418
JX513979 / KC447419
JX513980 / KC447420
KC447421
KC447422
KC447423
KC447424
Wolbachia / KC447445
KC447446
KC447447
KC447448
Coelostomidiapilosa / JX513982 / JX513990 / E-symbiont / KC447387
JX513991 / KC447388
JX513994 / KC447389
KC447390
S-symbiont / KC447397
KC447398
KC447399
KC447400
KC447401
KC447402
KC447403
KC447404
KC447405
KC447406
KC447407
KC447408
KC447409
Wolbachia / KF697119
Coelostomidiadeboerae / JX513969 / JX513986 / HC / KC447415
KC447416
Wolbachia / KC447444
Coelostomidiazealandica / JX513970 / JX513993 / HC / KC447427
KC447428
JX513971
JX513972 / KC447429
JX513973 / KC447430
KC447431
KC447432
KC447433
KC447434
KC447435
KC447436
Wolbachia / KC447450
KC447451
KC447452
KC447453
Ultracoelostomaassimile / JX513983 / JX513995 / S-symbiont / KC447410
JX513996 / KC447411
KC447412
KC447413
KC447414
Ultracoelostomabrittini / JX513974 / JX513997 / HC / KC447437
JX513981 / JX513998 / KC447438
JX513984 / JX513999 / KC447439
JX514000 / KC447440
JX514001 / KC447441
JX514002 / KC447442
Ultracoelostomadracophylli / JX513967 / JX514003 / HC / KC447443
Sequences from other families / GenBank accession numbers / Labels for Figures 2 & 3
Organism / 28 rRNA / 16S rRNA / COI / Host (28S rRNA sequences) / Symbiont (16S rRNA sequences)
Aonidiella aurantii / DQ145289 / DQ868798 / HM474070 / Aonidiella / Uzinura/Uzinura diaspidicola
Aonidomytilus ceanothi / DQ145305 / DQ868806 / - / Aonidomytilus / Uzinura/Uzinura diaspidicola
Aspidiotus nerii / GU213890 / DQ868800 / HM474081 / Aspidiotus / Uzinura/Uzinura diaspidicola
Aulacaspis yasumatsui / DQ145300 / DQ868803 / HM474091* / Aulacaspis / Uzinura/Uzinura diaspidicola
Aulacaspis sp. / - / DQ868802 / HM474089* / NA / NA
Blattidae sp. B03 / AY491209 / AM182898 / JN301639 / Blattidae / Blattabacterium
Carulaspis juniperi / DQ145301 / DQ868804 / - / Carulaspis / Uzinura/Uzinura diaspidicola
Chionaspis lepineyi / DQ145307 / DQ868809 / - / Chionaspis sp. 2 / Uzinura/Uzinura diaspidicola
Chionaspis pinifoliae / GQ325461 / DQ868807 / - / Chionaspis sp. 1 / Uzinura/Uzinura diaspidicola
Chrysomphalus aonidum / DQ145309 / DQ868810 / HM474096 / Chrysomphalus / Uzinura/Uzinura diaspidicola
Coleomegilla maculata / FJ687713 / Y13889 / EF192134 / Coccinellidae / male-killing symbiont
Diaspidiotus sp. / DQ145313 / DQ868813 / HM474135 / Diaspidiotus / Uzinura/Uzinura diaspidicola
Diaspis coccois / DQ145317 / DQ868816 / - / Diaspis sp. 1 / Uzinura/Uzinura diaspidicola
Diaspis echinocacti / GQ325477 / DQ868817 / - / Diaspis sp. 2 / Uzinura/Uzinura diaspidicola
Diaspis manzanitae / DQ145319 / DQ868818 / - / Diaspis sp. 3 / Uzinura/Uzinura diaspidicola
Duplachionaspis noaeae / DQ145321 / DQ868819 / - / Duplachionaspis / Uzinura/Uzinura diaspidicola
Fiorinia theae / DQ145332 / DQ868859 / - / Fiorinia / Uzinura/Uzinura diaspidicola
Hemiberlesia lataniae / DQ145340 / DQ868814 / HQ179913 / Hemiberlesia / Uzinura/Uzinura diaspidicola
Homalodisca lacerta / AF304669 / AY147399 / - / Cicadellidae / Uzinura/Uzinura diaspidicola
Howardia biclavis / DQ145345 / DQ868826 / - / Howardia / Uzinura/Uzinura diaspidicola
Icerya sp. / EU087785 / DQ868792 / - / Icerya / symbiont
Ischnaspis longirostris / DQ145350 / DQ868829 / - / Ischnaspis / Uzinura/Uzinura diaspidicola
Lepidosaphes pinnaeformis / DQ145354 / DQ868833 / - / Lepidosaphes / Uzinura/Uzinura diaspidicola
Leucaspis ohakunensis / DQ145356 / DQ868796 / - / Leucaspis / Uzinura/Uzinura diaspidicola
Melanaspis bromiliae / DQ145395 / DQ868844 / - / Melanaspis / Uzinura/Uzinura diaspidicola
Oceanaspidiotus spinosus / DQ145287 / DQ868837 / HM474235* / Oceanaspidiotus / Uzinura/Uzinura diaspidicola
Palinaspis quohogiformis / DQ145348 / DQ868828 / - / Palinaspis / Uzinura/Uzinura diaspidicola
Parlatoria camelliae / DQ145371 / DQ868841 / HM474248 / Parlatoria sp. 2 / Uzinura/Uzinura diaspidicola
Parlatoria pergandii / DQ145372 / DQ868842 / HM474247 / Parlatoria sp. 1 / Uzinura/Uzinura diaspidicola
Pinnaspis aspidistrae / DQ145374 / DQ868845 / HM474276* / Pinnaspis sp. 2 / Uzinura/Uzinura diaspidicola
Pinnaspis hibisci / DQ145376 / DQ868848 / HM474275* / Pinnaspis sp. 1 / Uzinura/Uzinura diaspidicola
Prodigiaspis sp. / DQ145379 / DQ868793 / - / Prodigiaspis / Uzinura/Uzinura diaspidicola
Protargionia larreae / DQ145380 / DQ868794 / - / Protargonia / Uzinura/Uzinura diaspidicola
Pseudaonidia trilobitiformis / DQ145382 / GQ424927 / HM474321 / Pseudaonidia / Uzinura/Uzinura diaspidicola
Pseudaulacaspis pentagona / DQ145385 / DQ868852 / HM474343 / Pseudaulacaspis sp. 2 / Uzinura/Uzinura diaspidicola
Pseudaulacaspis prunicola / DQ145386 / DQ868853 / HM474346 / Pseudaulacaspis sp. 1 / Uzinura/Uzinura diaspidicola
* denotes the host sequences where symbiont sequences from the nearest related species (within the same genus) was used since symbiont data were not available