Supplementary Table 1. PCR Primer References and Protocols for the Amplification and Sequencing

Supplementary Table 1. PCR primer references and protocols for the amplification and sequencing of the Y-STR loci examined in this study. For PCR conditions * denotes a PCR performed on Palm-Cycler (Corbett Research, Cambridge, UK) and # for Bio-Rad C1000™ Thermal Cycler or a Labnet MultiGene™ Gradient Thermal Cycler (Labnet International, Inc).

Locus / Reference / PCR Reagents (to a final volume of 25 µL) / PCR Conditions
DYS385ab / [1] / Two extracted discs, 1 X GoTaq® Flexi Buffer, 2.0 mM MgCl2 solution, 0.2 mM each dNTP, 1.5 U of GoTaq® DNA Polymerase, 0.3 µM of each primer / # 95 °C, 2 min
35 cycles:
94°C(30s), 55°C(30s), 72°C(30s)
72°C, 5 min
DYS392 / [1] / Two extracted discs, 1 X GoTaq® Flexi Buffer, 2.0 mM MgCl2 solution, 0.2 mM each dNTP, 1.5 U of GoTaq® DNA Polymerase, 0.3 µM of each primer / # 95 °C, 2 min
35 cycles:
94°C(30s), 56°C(30s), 72°C(30s)
72°C, 5 min
DYS456 / [2] / 0.1–5 ng of genomic DNA, 1X PCR Gold buffer, 1.5 mM MgCl2, 200 µM of each dNTP, 1.5U of AmpliTaq GoldTM Taq polymerase (Applied Biosystems), 0.3 µM of each primer / * 95°C, 9 min
30, 34 or 40 cycles:
94°C(50s), 55°C(50s), 72°C(25s)
72°C, 3 min
DYS458 / [3] / Two extracted discs, 1 X GoTaq® Flexi Buffer, 2.0 mM MgCl2 solution, 0.2 mM each dNTP, 1.5 U of GoTaq® DNA Polymerase, 0.3 µM of each primer / # 95 °C, 2 min
35 cycles:
94°C(30s), 62°C(30s), 72°C(30s)
72°C, 5 min
DYS635 / [4] / 0.1–5 ng of genomic DNA, 1X PCR Gold buffer, 2 mM MgCl2, 200 µM each dNTP, 1.5U of AmpliTaq Gold® Taq polymerase (Applied Biosystems,), 0.3 µM of each primer. / * 95°C, 9 min
32, 34 or 40 cycles:
94°C(10s), 68°C(15s), 72°C(25s)
72°C, 3 min
Y-GATA-H4 / [4] / Two extracted discs, 1 X GoTaq® Flexi Buffer, 2.0 mM MgCl2 solution, 0.2 mM each dNTP, 1.5 U of GoTaq® DNA Polymerase, 0.3 µM of each primer / # 95 °C, 2 min
35 cycles:
94°C(30s), 62°C(30s), 72°C(30s)
72°C, 5 min

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