Supplementary Table 1 Optimization of the Immobilizations of Ilva, Leudh and FDH A

Supplementary Table 1 Optimization of the immobilizations of IlvA, LeuDH and FDH a

Resins / Enzyme b / Protein dose per gram of resin (mg) / Immobilization yield (%) / Immobilization activity per gram of resin c (U)
epoxy resin, IlvA / 50 / 99.3 / 1027
100 / 86.3 / 1204
150 / 61.2 / 1868
amino resin, IlvA / 50 / 99.6 / 894
100 / 83.4 / 1874
150 / 58.6 / 1657
epoxy resin, FDH / 50 / 97.6 / 15
100 / 86.8 / 14
150 / 65.2 / 17
amino resin, FDH / 50 / 95 / 10
100 / 81 / 11
150 / 62 / 14
epoxy resin, LeuDH / 50 / 99.3 / 17
100 / 87.2 / 24
150 / 60.1 / 65
amino resin, LeuDH / 50 / 99.6 / 15
100 / 90.4 / 23
150 / 56.2 / 32

a IlvA, LeuDH and FDH refer to L-threonine deaminase, L-leucine dehydrogenase and formate dehydrogenase, respectively.

b Epoxy resin or amino resin was used for the immobilization of IlvA, LeuDH or FDH.

c refers to the activity of immobilized enzyme per gram of dry epoxy resin or wet amino resin. All assays were performed in triplicate and p values <0.05.

Supplementary Table 2 Optimization of the co-immobilizations of IlvA, LeuDH and FDH a

FDH, LeuDH and IlvA dosages per gram of dry resin b (mg) / FDH activity per gram of dry resin (U) / LeuDH activity per gram of dry resin (U) / IlvA activity per gram of dry resin (U)
50, 50, 0 / 18 / 25 / -
50, 100, 0 / 16 / 27 / -
100, 50, 0 / 24 / 19 / -
50, 0, 50 / 21 / - / 123
50, 0, 100 / 19 / - / 145
100, 0, 50 / 29 / - / 68
0, 50, 50 / - / 28 / 43
0, 100, 50 / - / 31 / 32
0, 50, 100 / - / 24 / 51
50, 50, 50 / 26 / 18 / 54
100, 50, 50 / 24 / 22 / 27
50, 50, 100 / 32 / 28 / 48
100, 100, 50 / 24 / 24 / 29
50, 100, 100 / 18 / 15 / 38

a IlvA, LeuDH and FDH refer to L-threonine deaminase, L-leucine dehydrogenase and formate dehydrogenase, respectively.

b IlvA, LeuDH and FDH were co-immobilized in the given proportion.

All assays were performed in triplicate and p values <0.05.