Supplementary table 1. Nucleotide sequences of primers used for PCR amplification in PBMCs and liver.
Gene / Forward primer(5’ to 3’) / Reverse primer
(5’ to 3’) / Ref. or Acc. No.
Abca1 / TTGGATGGATTATATTGGACTGC / TGGTCTCATTGAAAGCTTCTCTC / (1)
Abcg1 / CTTCTCCATGCTGTTCCTCATG / GCCAGGTAGTAGGCCTTCAG / *
Acadvl / AGATGAGTGCATCCAGATAATGG / AATGTCATTTGTCCCTTCAAAGA / (1)
Acat1 / AGCAAGATGAAGCCCAGAGA / CCATTGTCCAGAGATGCAGA / *
Acat2 / GGTGGAATTATGTGGCCAAGA / CATGTTGGCAAAGACAGGGAC / (2)
Acc / ACACTGGCTGGCTGGACAG / CACACAACTCCCAACATGGTG / (3)
Atgl / CACTTTAGCTCCAAGGATGA / TGGTTCAGTAGGCCATTCCT / (4)
β-actin / ACGTCGACATCCGCAAAGACCTC / TGATCTCCTTCTGCATCCGGTCA / (5)
Cpt1β / CCCACAGACCCAGGAACTT / GAAGGCGAACACAGATAGCC / AY762567.1
Dgat2 / TACAAGCAGGTGATCTTTGAGG / GGGCGAAACCAATATACTTCT / (1)
Fabppm / GGAGCCAGTTGCAAAG / ACGGCTCCTGGTCAAA / *
Fas / AGCCCCTCAAGTGCACAGTG / TGCCAATGTGTTTTCCCTGA / (1)
Gyk / CCTCTCTACAATGCCGTGGT / TCAATGGTCCCAAAAAGAGC / *
Gpat / GCAGACATCTGCTTCACCAA / GCAGGATGATGGGGTTTAGA / (6)
Hmgcr / CGAAGGGTTTGCAGTGATAAAGGA / GCCATAGTCACATGAAGCTTCTGTA / (7)
Hmgcs1 / CAGCTCTTTCACCATGCCTGG / TACTTTCCAGCATCTACACCATC / *
Hsl / GGTGACACTCGCAGAAGACAATA / GCCGCCGTGCTGTCTCT / (4)
Ldlr / GCATCACACTAGATCTTCCCAGT / GAGTTTGGAATCAACCCAATAGA / (1)
Lss / CCCTGAACTATGTGGCTCT / ATAGGGTGTTGAGTCCTTCC / (8)
Pparγ / CTCACGAAGAGCCTTCCAAC / GGATCCGGCAGTTAAGATCA / AB525757.1
Pparα / GTGGCTGCTATAATTTGCTGTG / AGCTTCGGGAAGAGAAAGGTAT / (1)
Scd1 / ACATGTCTGACCTGAAAGCTGA / GTACCTCTGGAACATCAC / (1)
Srb1 / AGGAGCATTCCTTGTTCCTAGAC / CAGGACTACTGGCTCGATCTTC / (1)
Srebp1 / GCGGACGCAGTCTGGG / ATGAGCTGGAGCATGTCTTCAAA / (1)
Ucp2 / GGTCGGAGATACCAGAGCAC / ATGAGGTTGGCTTTCAGGAG / *
Hamsters were fed with a standard diet (STD) or a high fat diet (HFD) diet during 30 days and received a daily oral dose of GSPE (25 mg per kg body weight) or the vehicle during the last 15 days. The table shows the nucleotide sequences of primers used for PCR amplification in PBMCs and liver. Abca1, ATP-binding cassette, sub-family A (ABC1), member 1; Abcg1, ATP-binding cassette, sub-family G (WHITE), member 1; Acadvl, acyl-CoA dehydrogenase, very long chain; Acat1, Acat1 acetyl-CoA acetyltransferase 1; Acat2, Acat2 acetyl-CoA acetyltransferase 2; Acc, acetyl-Coenzyme A carboxylase; Atgl, adipose triglyceride lipase; β-actin, beta-actin; Cpt1β, carnitine palmitoyltransferase I beta; Dgat2, diacylglycerol acyltransferase-2; Fabppm: fatty acid binding protein plasma membrane; Fas, fatty acid synthase; Gyk: glycerol kinase; Gpat, glycerol-3-phosphate acyltransferase; Hmgcr, 3-hydroxy-3-methylglutaryl-CoA reductase; Hmgcs1, 3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble); Hsl, hormone-sensitive lipase; Ldlr, low density lipoprotein receptor; Lss, lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase); Pparα, peroxisome proliferator-activated receptor alpha; Pparγ, peroxisome proliferator-activated receptor gamma; Scd1, stearoyl-CoA desaturase-1; Srb1, scavenger receptor class B, member 1; Srebp1, sterol regulatory element-binding protein; Ucp2, uncoupling protein 2 (mitochondrial, proton carrier). Primer pairs for PCR were designed using Primer3 software and sequence information obtained from Genbank. *No hamster sequence available. In these cases primers were designed to highly conserved regions determined by multiple sequence alignments performed on mice, rats and humans.
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