Supplementary Table 1: Details of the primers used for RT-PCR analysis (A) and Relative expression studies, Copy number assessment by Real time PCR (B) Amplification of 3’ region of PXMP-4 gene (C)

S. No. / Transcripts ID / Primer sequence (5’ - 3’) for Real Time PC
Forward primer Reverse Primer / Amplicon size (bp)
(A) List of primers designed for RT- PCR
33.6 / CCTCCAGCCCTCCTCCAGCCCT / CCTCCAGCCCTCCTCCAGCCCT / 22
Dp1 / TCTGTGATGGTCAGCTCGTC / ATCAGGTGTGAATGGGGGTA / 113
Dp2 / CCTCTGAGGGGCTCTTCC / ACTGTACGACCAAGGCAAGG / 157
Dp3 / AGGAGAGAGGCGAGCAGAG / TGCCACAAAGCTTGAGTCAT / 157
Dp4 / CTCCAGCCCTCATCTCAAGT / CCTGCAGAGTGCATGTGAGT / 211
Dp5 / TGAAGCAGCCCAGATTCTCT / CCCCTTGAAAAAGTGGTGTG / 201
Dp6 / CCCTGGGGGTACGTTAAAAT / TGTTCGTCTTACACCCGACA / 226
Dp7 / CTCCTCCAGCCCTCTTTACCT / GAGCCCTCTAAGTGGCAGTG / 301
Dp8 / CCAGAAACCAAGGTCAGAGC / AACCAGGGAAGGTGAGGATT / 220
Dp9 / TCCTCCAGCCCTAACACCA / AGCCCTCCTCACCCTGAA / 346
Dp10 / AGGGTTGTCACCACTTCCTG / AGGAACCACTCCTTCCCACT / 213
Dp11 / TGTCAATGCTGAAGCCAGAG / TCCCTTCTCAGGCAGTCCTA / 217
Dp12 / CCTCCAGCCCTTTATGTTCA / CAGCCATAAAAAGGAGCACA / 217
Dp13 / GGCGCATTAAATGTTCACC / CCTCCAGCCCTCACTACAAG / 192
Dp14 / TGGCTTAATCTTCCCGTTTG / GCACACTCCCCTCTAGTCCA / 233
Dp15 / TTAAGAACGTGGGCTTGCTT / AATTTCCTGGGGAATTGGAG / 153
Dp16 / CGCGAGATTTACACCCTCTC / CCGGGGAGAGTTCTCTTTTC / 159
Dp17 / GTGCAGAAGGAGCTGAGAGG / AGGGGTTGGGACAAGTCTCT / 188
Dp18 / CTGGGCTGGGAAACACTAAA / CTCCAGCCCTACTCAGATGG / 322
Dp19 / TCCAGCCCTTTCCTGGGTAA / CAGCCCTCTTCAGCTTCCA / 438
Dp20 / AGAAGCAGCGATCTGAGGAG / ACTCTGAGCAGGGATCTCCA / 234
Dp21 / CACTGAGTCGGCACTTGTGT / CTGTCCATGGGATTTTCCAG / 204
Dp22 / AGAGGATGTCGGTGAAGGTG / TTTGGCCTCTCTCTTCTCCA / 154
Dp23 / CCCTAGGATTCCAAGCAACA / TATTTTTGGCCGTGCTAGGT / 513
Dp24 / ACGCCCACTCCATTATCTTG / CCTGTTTCTCTGAGGGCTGT / 503
Dp25 / AAAGGAAGACGAGGCACTGA / CATCCCCAACAGCCACTATG / 540
Dp26 / CCTGTGAACCATGGAAACG / ATCCGTGTCTACCACCCTCA / 516
Dp27 / AGCCAGAGGGTCTTTCCAAG / CTGCAGGCGACGGATACTAC / 702
Dp28 / AGCCCTTTCTCCTCACCTCT / ATCCACTCCTGCAAAACAGG / 866
Dp29 / CCTCTTCATCAGGACCCTTG / GCCCTGATTCTCTCGTGAAG / 875
CD45 / GACATCGCAGTGTTTGTTGC / GGAGGTTCACATTCCTCTCG / 239
CDH1 / TCTACAGCATCACTGGCCAACGAGCTG / TGCTTGGACCATCAGGGTGTATGTGGG / 476
(B) List of primers designed for Real time PCR
Dp1 / GCCCTCGCCCTCTGTGAT / AACCAGTCCTGAACTCTTCTTTGTG / -
Dp2 / GGCTGCCTCCCCCTCTGA / AAGGTCATTGTTGCCTGTTTGA / -
Dp4 / CTTCCGGTCCCCTACTCACA / CTTTCTGGCTGTCATGAACAGAA / -
Dp8 / CCTGGTTCTGCTTACCTTCTTACTCT / GCAGGTTATGATTGCTCTAAGTTCTT / -
Dp9 / CATCTGGACAACGAGGAGAATG / GACGCCGACTGAATGAATGA / -
Dp10 / TGTCACCACTTCCTGGAGCTT / GTGGACATGAAGAAGGTGGATGT / -
Dp16 / ACTTCCCTTACCTACATTGTTCCAA / CGGGCCGTACCCATATCC / -
Dp17 / ATGTAAGCGAGAAAGTGATGAGTGA / TCCCTGGAAAGCCTGGTCTA / -
Dp19 / GGAACCGGAAAAGAACCTTGCTGTT / TCCTTAACCCCACTCATCAAATAAC / -
Dp20 / TCCCGGCTCCACCTCATA / CAGGAAGGTTCACATCACAAAGAC / -
Dp22 / TGCTGCTAGCCACGAGGAAT / GAGATGAGAACTTTGGCACAATCA / -
Dp26 / GAGGGTGGTAGACACGGATCA / ACTGAGGGTTGTGCAATAGCAA / -
Dp27 / AGCATCCAAGGGCCTTCTG / CATCCTGTGAGCGTTGACTGA / -
GAPDH / GCAAGTTCCACGGCACAGT / GATGGTGATGGCCTTTCCAT
(C) Primers used for amplification of 3’ region of BuffaloPXMP-4 gene
1. / Dp30 / GCTGTGGCTCTTCGAGTACC / CATTTGAGAAATACTGGGTTCAAA / 883