Supplementary Table 1. Coordinates for species-sites (datum = WGS84) included in the distribution models. PNE, Pleurodeles nebulosus; PPO, Pleurodeles poireti; Long, Longitude; Lat, Latitude.

Long / Lat / Long / Lat / Long / Lat / Long / Lat
PNE / 9.68 / 37.04 / 9.71 / 37.24 / 8.51 / 36.51 / 9.62 / 37.20
9.94 / 36.92 / 2.69 / 36.36 / 9.41 / 36.68 / 8.70 / 36.67
8.75 / 36.92 / 9.21 / 37.07 / 9.08 / 36.96 / 6.95 / 36.79
8.98 / 36.61 / 9.82 / 37.03 / 10.72 / 36.69 / 8.69 / 36.57
10.84 / 36.90 / 10.78 / 36.71 / 9.14 / 37.07 / 7.05 / 36.76
8.76 / 36.93 / 9.19 / 37.10 / 9.14 / 37.07 / 8.31 / 36.49
10.84 / 36.72 / 9.72 / 37.11 / 9.21 / 37.11 / 8.37 / 36.77
8.02 / 36.83 / 9.72 / 37.24 / 9.10 / 37.01 / 9.52 / 37.20
9.23 / 37.05 / 9.77 / 37.09 / 8.76 / 36.90 / 6.13 / 36.80
9.79 / 37.25 / 8.52 / 36.50 / 9.09 / 37.05 / 9.06 / 37.04
9.10 / 36.93 / 8.58 / 36.87 / 8.33 / 36.55 / 1.97 / 35.86
Long / Lat / Long / Lat / Long / Lat
PPO / 7.15 / 36.89 / 7.71 / 36.84 / 7.32 / 36.99
7.57 / 36.92 / 7.72 / 36.87 / 7.38 / 36.80
7.39 / 37.02 / 7.51 / 36.86 / 7.32 / 36.94
7.17 / 36.90 / 7.60 / 36.82 / 7.53 / 36.83
7.68 / 36.68 / 7.60 / 36.82 / 7.32 / 36.95
7.62 / 36.67 / 7.52 / 36.88 / 7.39 / 36.86
7.98 / 36.75 / 7.34 / 36.80 / 7.46 / 36.88
7.31 / 36.86 / 7.51 / 36.72

Supplementary Table 2.Sample codes, species assignment based on the combined molecular dataset, locality information, including latitude and longitude, and results of molecular analyses. mtDNA refers to individual assignment based on the coalescent-based analysis implemented in BEAST (nW, nC, and nE refer to the West, Central and East clades in P. nebulosus, and p to P. poireti). Values in the final three columns represent the probability of assignment to the P. nebulosus genetic cluster (= pnebulosus) based on the results of Structure and New Hybrids (NH) analyses (in the last column, phybrid is the cumulative frequency of belonging to any of the hybrid classes, including F1, F2 and the two backcrosses with pure parentals, only shown when > 0.5).

Code / Species / Code / Locality / Country / Latitude / Longitude / mtDNA / msats (Structure. pnebulosus) / msats (NH. pnebulosus) / msats (NH. phybrid)
IMS5137 / nebulosus / 1 / Khiri oued Adjoul / Algeria / 36.8 / 6.13 / nW / 0.9775 / 0.9653
IMS5138 / nebulosus / Khiri oued Adjoul / Algeria / 36.8 / 6.13 / nW / 0.9812 / 0.9710
IMS5094 / nebulosus / 2 / Djendel / Algeria / 36.79 / 6.95 / nW / 0.9900 / 0.9922
IMS5095 / nebulosus / Djendel / Algeria / 36.79 / 6.95 / nW / 0.9896 / 0.9921
IMS5096 / nebulosus x poireti / Djendel / Algeria / 36.79 / 6.95 / nW / 0.8575 / 0.3696 / 0.63044
IMS5097 / nebulosus / Djendel / Algeria / 36.79 / 6.95 / nW / 0.9799 / 0.9762
IMS5098 / nebulosus x poireti / 3 / Djendel 2 / Algeria / 36.76 / 7.05 / nW / 0.7491 / 0.0009 / 0.99906
IMS5099 / nebulosus x poireti / Djendel 2 / Algeria / 36.76 / 7.05 / nW / 0.6071 / 0.0000 / 0.99997
IMS5100 / nebulosus x poireti / Djendel 2 / Algeria / 36.76 / 7.05 / nW / 0.8304 / 0.2082 / 0.7918
IMS5101 / nebulosus x poireti / Djendel 2 / Algeria / 36.76 / 7.05 / nW / 0.7604 / 0.0101 / 0.98988
IMS5102 / nebulosus / Djendel 2 / Algeria / 36.76 / 7.05 / nW / 0.9316 / 0.6914
IMS5103 / nebulosus x poireti / Djendel 2 / Algeria / 36.76 / 7.05 / nW / 0.5530 / 0.0000 / 0.99999
IMS5104 / nebulosus x poireti / Djendel 2 / Algeria / 36.76 / 7.05 / nW / 0.6341 / 0.0001 / 0.99995
IMS5105 / nebulosus x poireti / Djendel 2 / Algeria / 36.76 / 7.05 / nW / 0.6758 / 0.0027 / 0.99727
IMS5106 / nebulosus x poireti / Djendel 2 / Algeria / 36.76 / 7.05 / nW / 0.6087 / 0.0219 / 0.97811
IMS5107 / nebulosus x poireti / Djendel 2 / Algeria / 36.76 / 7.05 / nW / 0.9033 / 0.0522 / 0.94779
IMS5108 / nebulosus x poireti / Djendel 2 / Algeria / 36.76 / 7.05 / nW / 0.8400 / 0.0041 / 0.99594
IMS5109 / nebulosus x poireti / Djendel 2 / Algeria / 36.76 / 7.05 / nW / 0.1675 / 0.0000 / 0.9101
IMS5110 / nebulosus x poireti / Djendel 2 / Algeria / 36.76 / 7.05 / nW / 0.5887 / 0.0010 / 0.99899
IMS5111 / nebulosus x poireti / Djendel 2 / Algeria / 36.76 / 7.05 / nW / 0.5383 / 0.0039 / 0.99611
IMS5112 / nebulosus x poireti / Djendel 2 / Algeria / 36.76 / 7.05 / nW / 0.6167 / 0.0019 / 0.99813
IMS5113 / nebulosus x poireti / Djendel 2 / Algeria / 36.76 / 7.05 / nW / 0.5340 / 0.0000 / 0.99998
IMS5114 / nebulosus x poireti / Djendel 2 / Algeria / 36.76 / 7.05 / nW / 0.5110 / 0.0529 / 0.94286
IMS5093 / poireti / 4 / Filfila, Dam El Bagrat / Algeria / 36.89 / 7.15 / p / 0.0444 / 0.0000
IMS5092 / poireti / 5 / Plage Gribes / Algeria / 36.9 / 7.17 / p / 0.0110 / 0.0000
IMS5052 / poireti / 6 / Draa Elrich 4 / Algeria / 36.86 / 7.31 / 0.0050 / 0.0000
IMS5053 / poireti / Draa Elrich 4 / Algeria / 36.86 / 7.31 / 0.0060 / 0.0000
IMS5054 / poireti / Draa Elrich 4 / Algeria / 36.86 / 7.31 / 0.0070 / 0.0000
IMS5055 / poireti / 7 / Sidi Ali Ben Ali 1 / Algeria / 36.94 / 7.32 / p / 0.0050 / 0.0000
IMS5056 / poireti / Sidi Ali Ben Ali 1 / Algeria / 36.94 / 7.32 / 0.0070 / 0.0000
IMS5057 / poireti / 8 / Sidi Ali Ben Ali 2 / Algeria / 36.95 / 7.32 / p / 0.0046 / 0.0000
IMS5058 / poireti / Sidi Ali Ben Ali 2 / Algeria / 36.95 / 7.32 / 0.0062 / 0.0000
IMS5059 / poireti / 9 / Azzla / Algeria / 36.99 / 7.32 / p / 0.0042 / 0.0000
IMS5060 / poireti / Azzla / Algeria / 36.99 / 7.32 / 0.0050 / 0.0000
IMS5061 / poireti / Azzla / Algeria / 36.99 / 7.32 / 0.0060 / 0.0000
IMS5062 / poireti / Azzla / Algeria / 36.99 / 7.32 / 0.0053 / 0.0000
IMS5063 / poireti / Azzla / Algeria / 36.99 / 7.32 / 0.0050 / 0.0000
IMS5064 / poireti / Azzla / Algeria / 36.99 / 7.32 / 0.0050 / 0.0000
IMS5065 / poireti / Azzla / Algeria / 36.99 / 7.32 / 0.0130 / 0.0000
IMS5066 / poireti / Azzla / Algeria / 36.99 / 7.32 / 0.0060 / 0.0000
IMS5067 / poireti / Azzla / Algeria / 36.99 / 7.32 / 0.0080 / 0.0000
IMS5068 / poireti / Azzla / Algeria / 36.99 / 7.32 / 0.0047 / 0.0000
IMS5069 / poireti / Azzla / Algeria / 36.99 / 7.32 / 0.0050 / 0.0000
IMS5070 / poireti / Azzla / Algeria / 36.99 / 7.32 / 0.0050 / 0.0000
IMS5071 / poireti / Azzla / Algeria / 36.99 / 7.32 / 0.0080 / 0.0000
IMS5072 / poireti / Azzla / Algeria / 36.99 / 7.32 / 0.0070 / 0.0000
IMS5073 / poireti / Azzla / Algeria / 36.99 / 7.32 / 0.0040 / 0.0000
IMS5074 / poireti / Azzla / Algeria / 36.99 / 7.32 / 0.0050 / 0.0000
IMS5075 / poireti / Azzla / Algeria / 36.99 / 7.32 / 0.0050 / 0.0000
IMS5076 / poireti / Azzla / Algeria / 36.99 / 7.32 / 0.0100 / 0.0000
IMS5077 / poireti / Azzla / Algeria / 36.99 / 7.32 / 0.0050 / 0.0000
IMS5078 / poireti / Azzla / Algeria / 36.99 / 7.32 / 0.0050 / 0.0000
IMS5079 / poireti / Azzla / Algeria / 36.99 / 7.32 / 0.0050 / 0.0000
IMS5080 / poireti / Azzla / Algeria / 36.99 / 7.32 / 0.0155 / 0.0000
IMS5119 / poireti / 10 / Lac Fatzara / Algeria / 36.8 / 7.38 / p / 0.0040 / 0.0000
IMS5120 / poireti / Lac Fatzara / Algeria / 36.8 / 7.38 / 0.0040 / 0.0000
IMS5048 / poireti / 11 / Draa Elrich 3 / Algeria / 36.86 / 7.39 / 0.0090 / 0.0000
IMS5049 / poireti / Draa Elrich 3 / Algeria / 36.86 / 7.39 / 0.0181 / 0.0000
IMS5050 / poireti / Draa Elrich 3 / Algeria / 36.86 / 7.39 / 0.0218 / 0.0000
IMS5051 / poireti / Draa Elrich 3 / Algeria / 36.86 / 7.39 / 0.0079 / 0.0000
IMS5081 / poireti / 12 / Chetaibi 2 / Algeria / 37.02 / 7.39 / p / - / -
IMS5082 / poireti / Chetaibi 2 / Algeria / 37.02 / 7.39 / 0.0042 / 0.0000
IMS5083 / poireti / Chetaibi 2 / Algeria / 37.02 / 7.39 / 0.0050 / 0.0000
IMS5084 / poireti / Chetaibi 2 / Algeria / 37.02 / 7.39 / 0.0040 / 0.0000
IMS5085 / poireti / Chetaibi 2 / Algeria / 37.02 / 7.39 / 0.0050 / 0.0000
IMS5043 / poireti / 13 / Draa Elrich 2 / Algeria / 36.88 / 7.46 / p / 0.0050 / 0.0000
IMS5044 / poireti / Draa Elrich 2 / Algeria / 36.88 / 7.46 / p / 0.0110 / 0.0000
IMS5045 / poireti / Draa Elrich 2 / Algeria / 36.88 / 7.46 / p / 0.0110 / 0.0000
IMS5046 / poireti / Draa Elrich 2 / Algeria / 36.88 / 7.46 / p / 0.0150 / 0.0000
IMS5047 / poireti / Draa Elrich 2 / Algeria / 36.88 / 7.46 / 0.0149 / 0.0000
IMS5038 / poireti / 14 / Draa Elrich 1 / Algeria / 36.86 / 7.51 / p / 0.0070 / 0.0000
IMS5039 / poireti / Draa Elrich 1 / Algeria / 36.86 / 7.51 / 0.0110 / 0.0000
IMS5040 / poireti / Draa Elrich 1 / Algeria / 36.86 / 7.51 / 0.0544 / 0.0000
IMS5041 / poireti / Draa Elrich 1 / Algeria / 36.86 / 7.51 / 0.0203 / 0.0000
IMS5042 / poireti / Draa Elrich 1 / Algeria / 36.86 / 7.51 / 0.0149 / 0.0000
IMS5121 / poireti / 15 / El Hashassia / Algeria / 36.72 / 7.51 / p / 0.0588 / 0.0000
IMS5122 / poireti / El Hashassia / Algeria / 36.72 / 7.51 / 0.0200 / 0.0000
IMS5123 / poireti / El Hashassia / Algeria / 36.72 / 7.51 / 0.0369 / 0.0000
IMS5124 / poireti / El Hashassia / Algeria / 36.72 / 7.51 / 0.0050 / 0.0000
IMS5116 / poireti / 16 / Aïn Barbar 3 / Algeria / 36.88 / 7.52 / p / - / -
IMS5117 / poireti / Aïn Barbar 3 / Algeria / 36.88 / 7.52 / p / 0.0170 / 0.0000
IMS5118 / poireti / Aïn Barbar 3 / Algeria / 36.88 / 7.52 / p / 0.0250 / 0.0000
IMS5037 / poireti / 17 / Cross to Chetaibi / Algeria / 36.83 / 7.53 / p / 0.0261 / 0.0000
IMS5115 / poireti / 18 / Aïn Barbar 2 / Algeria / 36.92 / 7.57 / p / 0.0340 / 0.0000
IMS5027 / poireti / 19 / Oued Zied 1+2 / Algeria / 36.82 / 7.6 / p / 0.0080 / 0.0000
IMS5028 / poireti / Oued Zied 1+2 / Algeria / 36.82 / 7.6 / p / 0.0922 / 0.0000
IMS5029 / poireti / Oued Zied 1+2 / Algeria / 36.82 / 7.6 / 0.0330 / 0.0000
IMS5030 / poireti / Oued Zied 1+2 / Algeria / 36.82 / 7.6 / 0.0130 / 0.0000
IMS5031 / poireti / Oued Zied 1+2 / Algeria / 36.82 / 7.6 / 0.0050 / 0.0000
IMS5032 / poireti / Oued Zied 1+2 / Algeria / 36.82 / 7.6 / 0.0070 / 0.0000
IMS5034 / poireti / Oued Zied 1+2 / Algeria / 36.82 / 7.6 / 0.0060 / 0.0000
IMS5035 / poireti / Oued Zied 1+2 / Algeria / 36.82 / 7.6 / 0.0111 / 0.0000
IMS5125 / poireti / 20 / Aïn Sayed / Algeria / 36.67 / 7.62 / p / 0.0111 / 0.0000
IMS5126 / poireti / 21 / 3 km to Drean / Algeria / 36.68 / 7.68 / p / - / -
IMS5021 / poireti / 22 / Bousedra / Algeria / 36.84 / 7.71 / p / 0.0672 / 0.0000
IMS5022 / poireti / Bousedra / Algeria / 36.84 / 7.71 / 0.0080 / 0.0000
IMS5023 / poireti / Bousedra / Algeria / 36.84 / 7.71 / 0.0060 / 0.0000
IMS5024 / poireti / Bousedra / Algeria / 36.84 / 7.71 / 0.0060 / 0.0000
IMS5025 / poireti / Bousedra / Algeria / 36.84 / 7.71 / 0.0170 / 0.0000
IMS5026 / poireti / Bousedra / Algeria / 36.84 / 7.71 / 0.0183 / 0.0000
IMS5036 / poireti / 23 / University Sidi Achour / Algeria / 36.87 / 7.72 / p / - / -
IMS5133 / nebulosus x poireti / 24 / Lac d'Oiseaux / Algeria / 36.75 / 7.98 / p / 0.1900 / 0.0000 / 0.98484
IMS5134 / nebulosus x poireti / Lac d'Oiseaux / Algeria / 36.75 / 7.98 / p / 0.1172 / 0.0000 / 0.92573
IMS5135 / poireti / Lac d'Oiseaux / Algeria / 36.75 / 7.98 / p / 0.0090 / 0.0000
IMS5136 / poireti / Lac d'Oiseaux / Algeria / 36.75 / 7.98 / p / 0.0090 / 0.0000
IMS5086 / nebulosus x poireti / 25 / Griat / Algeria / 36.83 / 8.02 / nC / 0.8506 / 0.0053 / 0.99466
IMS5087 / nebulosus x poireti / 26 / Berrihane / Algeria / 36.84 / 8.14 / nC / 0.8413 / 0.1230 / 0.877
IMS4994 / nebulosus / 27 / Aire protégée El Feïja / Tunisia / 36.49 / 8.31 / nC / - / -
IMS5018 / nebulosus / 28 / Aïn soltane (Sraa el Majen) / Tunisia / 36.56 / 8.34 / nC / 0.9728 / 0.9799
IMS5019 / nebulosus / Aïn soltane (Sraa el Majen) / Tunisia / 36.56 / 8.34 / nC / 0.9398 / 0.8987
IMS5091 / nebulosus x poireti / 29 / Aïn Al-Assel / Algeria / 36.77 / 8.37 / nC / 0.9025 / 0.1653 / 0.83473
IMS4980 / nebulosus / 30 / Edkhailia 2 / Tunisia / 36.51 / 8.51 / nC / 0.9890 / 0.9878
IMS4981 / nebulosus / Edkhailia 2 / Tunisia / 36.51 / 8.51 / 0.9799 / 0.9202
IMS4982 / nebulosus / Edkhailia 2 / Tunisia / 36.51 / 8.51 / 0.9883 / 0.9774
IMS4983 / nebulosus / Edkhailia 2 / Tunisia / 36.51 / 8.51 / 0.9910 / 0.9862
IMS4984 / nebulosus x poireti / Edkhailia 2 / Tunisia / 36.51 / 8.51 / 0.8673 / 0.0835 / 0.91651
IMS4985 / nebulosus / Edkhailia 2 / Tunisia / 36.51 / 8.51 / 0.9920 / 0.9943
IMS4986 / nebulosus / Edkhailia 2 / Tunisia / 36.51 / 8.51 / 0.9910 / 0.9880
IMS4987 / nebulosus / Edkhailia 2 / Tunisia / 36.51 / 8.51 / 0.9601 / 0.7423
IMS4988 / nebulosus / Edkhailia 2 / Tunisia / 36.51 / 8.51 / 0.9940 / 0.9979
IMS4989 / nebulosus / Edkhailia 2 / Tunisia / 36.51 / 8.51 / 0.9910 / 0.9886
IMS4990 / nebulosus / Edkhailia 2 / Tunisia / 36.51 / 8.51 / 0.9741 / 0.8434
IMS4991 / nebulosus / Edkhailia 2 / Tunisia / 36.51 / 8.51 / 0.9837 / 0.9655
IMS4992 / nebulosus / Edkhailia 2 / Tunisia / 36.51 / 8.51 / 0.9940 / 0.9968
IMS4993 / nebulosus / Edkhailia 2 / Tunisia / 36.51 / 8.51 / 0.9870 / 0.9683
IMS5088 / nebulosus x poireti / 31 / Laayoun / Algeria / 36.87 / 8.58 / nC / 0.9086 / 0.1626 / 0.83739
IMS5089 / nebulosus / Laayoun / Algeria / 36.87 / 8.58 / 0.9687 / 0.9754
IMS5090 / nebulosus x poireti / Laayoun / Algeria / 36.87 / 8.58 / 0.9408 / 0.4438 / 0.5562
IMS 1301 / nebulosus / 32 / Bouzzaroua (Fernana) / Tunisia / 36.68 / 8.71 / nC / - / -
IMS 1302 / nebulosus / Bouzzaroua (Fernana) / Tunisia / 36.68 / 8.71 / nC / - / -
IMS 1303 / nebulosus / Bouzzaroua (Fernana) / Tunisia / 36.68 / 8.71 / nC / - / -
IMS 1304 / nebulosus / Bouzzaroua (Fernana) / Tunisia / 36.68 / 8.71 / nC / - / -
IMS 1305 / nebulosus / Bouzzaroua (Fernana) / Tunisia / 36.68 / 8.71 / nC / - / -
IMS 1306 / nebulosus / Bouzzaroua (Fernana) / Tunisia / 36.68 / 8.71 / nC / - / -
IMS 1307 / nebulosus / Bouzzaroua (Fernana) / Tunisia / 36.68 / 8.71 / nC / - / -
IMS 1308 / nebulosus / Bouzzaroua (Fernana / Tunisia / 36.68 / 8.71 / nC / - / -
IMS4977 / nebulosus / Bouzzaroua (Fernana) / Tunisia / 36.68 / 8.71 / nC / 0.9680 / 0.8463
IMS4978 / nebulosus / Bouzzaroua (Fernana) / Tunisia / 36.68 / 8.71 / 0.9713 / 0.7359
IMS4979 / nebulosus / Bouzzaroua (Fernana) / Tunisia / 36.68 / 8.71 / 0.9930 / 0.9965
IMS4976 / nebulosus / 33 / Sandaj (Tabarka) / Tunisia / 36.92 / 8.75 / nE / 0.9892 / 0.9918
IMS4972 / nebulosus / 34 / Erbayhia 1 / Tunisia / 37.01 / 9.11 / nE / 0.9920 / 0.9943
IMS4973 / nebulosus / Erbayhia 1 / Tunisia / 37.01 / 9.11 / 0.9920 / 0.9953
IMS4974 / nebulosus / Erbayhia 1 / Tunisia / 37.01 / 9.11 / 0.9930 / 0.9967
IMS4975 / nebulosus / Erbayhia 1 / Tunisia / 37.01 / 9.11 / 0.9950 / 0.9986
IMS4958 / nebulosus / 35 / Al-Haniya / Tunisia / 37.11 / 9.21 / nE / 0.9930 / 0.9944
IMS4959 / nebulosus / Al-Haniya / Tunisia / 37.11 / 9.21 / 0.9891 / 0.9823
IMS4960 / nebulosus / Al-Haniya / Tunisia / 37.11 / 9.21 / 0.9920 / 0.9926
IMS4961 / nebulosus / Al-Haniya / Tunisia / 37.11 / 9.21 / 0.9940 / 0.9979
IMS4962 / nebulosus / Al-Haniya / Tunisia / 37.11 / 9.21 / 0.9950 / 0.9983
IMS4968 / nebulosus / 36 / Al-Maajen 1+ 2+ 3 / Tunisia / 37.08 / 9.21 / nE / 0.9950 / 0.9984
IMS4969 / nebulosus x poireti / Al-Maajen 1+ 2 + 3 / Tunisia / 37.08 / 9.21 / 0.9148 / 0.4021 / 0.59792
IMS4970 / nebulosus / Al-Maajen 1+ 2 + 3 / Tunisia / 37.08 / 9.21 / 0.9919 / 0.9948
IMS4971 / nebulosus / Al-Maajen 1+ 2 + 3 / Tunisia / 37.08 / 9.21 / 0.9920 / 0.9948
IMS4967 / nebulosus / 37 / Bessetine 1 / Tunisia / 37.06 / 9.23 / nE / 0.9930 / 0.9951
IMS4928 / nebulosus / 38 / Ksar Chikh+Bousselem / Tunisia / 36.69 / 9.42 / nC / 0.9800 / 0.9622
IMS4929 / nebulosus / Ksar Chikh+Bousselem / Tunisia / 36.69 / 9.42 / 0.9920 / 0.9932
IMS4931 / nebulosus / Ksar Chikh+Bousselem / Tunisia / 36.69 / 9.42 / 0.9880 / 0.9959
IMS4932 / nebulosus / Ksar Chikh+Bousselem / Tunisia / 36.69 / 9.42 / 0.9960 / 0.9996
IMS4933 / nebulosus / Ksar Chikh+Bousselem / Tunisia / 36.69 / 9.42 / 0.9612 / 0.7670
IMS4934 / nebulosus / Ksar Chikh+Bousselem / Tunisia / 36.69 / 9.42 / 0.9930 / 0.9979
IMS4935 / nebulosus / Ksar Chikh+Bousselem / Tunisia / 36.69 / 9.42 / 0.9949 / 0.9985
IMS4937 / nebulosus / Ksar Chikh+Bousselem / Tunisia / 36.69 / 9.42 / 0.9456 / 0.6373
IMS4940 / nebulosus / Ksar Chikh+Bousselem / Tunisia / 36.69 / 9.42 / 0.9821 / 0.9877
IMS4963 / nebulosus / 39 / Teskraia 2 (Tebraska) / Tunisia / 37.21 / 9.53 / nE / 0.9930 / 0.9966
IMS4964 / nebulosus / Teskraia 2 (Tebraska) / Tunisia / 37.21 / 9.53 / 0.9950 / 0.9990
IMS4965 / nebulosus / Teskraia 2 (Tebraska) / Tunisia / 37.21 / 9.53 / 0.9950 / 0.9990
IMS4966 / nebulosus / Teskraia 2 (Tebraska) / Tunisia / 37.21 / 9.53 / 0.9938 / 0.9975
IMS4953 / nebulosus / 40 / Bouhdid / Tunisia / 37.24 / 9.71 / nE / 0.9960 / 0.9994
IMS4954 / nebulosus / Bouhdid / Tunisia / 37.24 / 9.71 / 0.9950 / 0.9992
IMS4955 / nebulosus / Bouhdid / Tunisia / 37.24 / 9.71 / 0.9960 / 0.9997
IMS4956 / nebulosus / Bouhdid / Tunisia / 37.24 / 9.71 / 0.9940 / 0.9977
IMS4957 / nebulosus / Bouhdid / Tunisia / 37.24 / 9.71 / 0.9960 / 0.9996
IMS4950 / nebulosus / 41 / Bizerte 4+5 / Tunisia / 37.24 / 9.72 / 0.9950 / 0.9992
IMS4951 / nebulosus / Bizerte 4+5 / Tunisia / 37.24 / 9.72 / nE / - / -
IMS4952 / nebulosus / Bizerte 4+5 / Tunisia / 37.24 / 9.72 / 0.9940 / 0.9979
IMS5161 / nebulosus / 42 / Tinja / Tunisia / 37.09 / 9.77 / 0.9893 / 0.9880
IMS4944 / nebulosus / 43 / Bizerte 1 / Tunisia / 37.25 / 9.79 / nE / 0.9950 / 0.9995
IMS4945 / nebulosus / Bizerte 1 / Tunisia / 37.25 / 9.79 / 0.9950 / 0.9987
IMS4946 / nebulosus / Bizerte 1 / Tunisia / 37.25 / 9.79 / 0.9940 / 0.9977
IMS4947 / nebulosus / Bizerte 1 / Tunisia / 37.25 / 9.79 / 0.9950 / 0.9989
IMS5139 / nebulosus / 44 / 13 km to Menzel Bouzelfa (Cap Bon) / Tunisia / 36.67 / 10.7 / nE / 0.9920 / 0.9941
IMS5140 / nebulosus / 13 km to Menzel Bouzelfa (Cap Bon) / Tunisia / 36.67 / 10.7 / 0.9940 / 0.9980
IMS5141 / nebulosus / 13 km to Menzel Bouzelfa (Cap Bon) / Tunisia / 36.67 / 10.7 / 0.9950 / 0.9979
IMS5142 / nebulosus / 13 km to Menzel Bouzelfa (Cap Bon) / Tunisia / 36.67 / 10.7 / 0.9925 / 0.9936
IMS5143 / nebulosus / 13 km to Menzel Bouzelfa (Cap Bon) / Tunisia / 36.67 / 10.7 / 0.9950 / 0.9984
IMS5144 / nebulosus / 13 km to Menzel Bouzelfa (Cap Bon) / Tunisia / 36.67 / 10.7 / 0.9894 / 0.9913
IMS5145 / nebulosus / 13 km to Menzel Bouzelfa (Cap Bon) / Tunisia / 36.67 / 10.7 / 0.9908 / 0.9866
IMS5146 / nebulosus / 13 km to Menzel Bouzelfa (Cap Bon) / Tunisia / 36.67 / 10.7 / 0.9920 / 0.9950
IMS5147 / nebulosus / 13 km to Menzel Bouzelfa (Cap Bon) / Tunisia / 36.67 / 10.7 / 0.9880 / 0.9792
IMS5148 / nebulosus / 13 km to Menzel Bouzelfa (Cap Bon) / Tunisia / 36.67 / 10.7 / 0.9940 / 0.9976
IMS5149 / nebulosus / 13 km to Menzel Bouzelfa (Cap Bon) / Tunisia / 36.67 / 10.7 / 0.9920 / 0.9958
IMS5020 / nebulosus / 45 / Oued El Mida (Cap Bon) / Tunisia / 36.73 / 10.84 / nE / 0.9930 / 0.9934
IMS5150 / nebulosus / 46 / Enchaa (Cap Bon) / Tunisia / 36.9 / 10.84 / nE / - / -

Supplementary Table 3.Sample size (N), number of alleles (Na), observed (Ho), and expected heterozygosity (He) per locus in the 42 populations analysed using microsatellite markers.

Pop/locus / Ppl7 / Ppl5 / Pl2.3 / Ppl14 / Ppl13 / Ppl12 / Pl3.2 / Pl4.1 / Pl2.29 / Pl2.16 / Pl3.11 / Ppl10
1. Khiri oued Adjoul / N / 2 / 0 / 2 / 2 / 2 / 2 / 2 / 2 / 2 / 2 / 2 / 2
Na / 2 / 0 / 1 / 4 / 2 / 3 / 1 / 2 / 2 / 3 / 2 / 1
Ho / 0.500 / 0.000 / 0.000 / 1.000 / 0.500 / 1.000 / 0.000 / 1.000 / 0.500 / 1.000 / 0.000 / 0.000
He / 0.375 / 0.000 / 0.000 / 0.750 / 0.375 / 0.625 / 0.000 / 0.500 / 0.375 / 0.625 / 0.500 / 0.000
2. Djendel / N / 4 / 0 / 4 / 4 / 4 / 4 / 4 / 4 / 4 / 4 / 4 / 4
Na / 3 / 0 / 2 / 3 / 3 / 3 / 1 / 2 / 1 / 2 / 2 / 3
Ho / 1.000 / 0.000 / 0.000 / 1.000 / 1.000 / 0.750 / 0.000 / 0.750 / 0.000 / 0.500 / 0.250 / 0.750
He / 0.625 / 0.000 / 0.375 / 0.594 / 0.594 / 0.656 / 0.000 / 0.469 / 0.000 / 0.375 / 0.219 / 0.656
3. Djendel 2 / N / 17 / 9 / 17 / 17 / 17 / 17 / 17 / 17 / 17 / 17 / 17 / 17
Na / 7 / 3 / 2 / 11 / 9 / 8 / 2 / 4 / 3 / 5 / 4 / 7
Ho / 0.882 / 0.333 / 0.353 / 0.824 / 0.882 / 0.765 / 0.118 / 0.529 / 0.706 / 0.647 / 0.471 / 0.824
He / 0.813 / 0.586 / 0.457 / 0.825 / 0.830 / 0.787 / 0.111 / 0.548 / 0.590 / 0.654 / 0.471 / 0.789
4. Filfila, Dam El Bagrat / N / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1
Na / 2 / 2 / 1 / 2 / 1 / 2 / 1 / 1 / 1 / 2 / 1 / 2
Ho / 1.000 / 1.000 / 0.000 / 1.000 / 0.000 / 1.000 / 0.000 / 0.000 / 0.000 / 1.000 / 0.000 / 1.000
He / 0.500 / 0.500 / 0.000 / 0.500 / 0.000 / 0.500 / 0.000 / 0.000 / 0.000 / 0.500 / 0.000 / 0.500
5. Plage Gribes / N / 1 / 1 / 1 / 1 / 1 / 1 / 0 / 1 / 1 / 1 / 1 / 1
Na / 2 / 1 / 1 / 2 / 1 / 2 / 0 / 1 / 1 / 1 / 1 / 2
Ho / 1.000 / 0.000 / 0.000 / 1.000 / 0.000 / 1.000 / 0.000 / 0.000 / 0.000 / 0.000 / 0.000 / 1.000
He / 0.500 / 0.000 / 0.000 / 0.500 / 0.000 / 0.500 / 0.000 / 0.000 / 0.000 / 0.000 / 0.000 / 0.500
6. Draa Elrich 4 / N / 3 / 3 / 3 / 3 / 1 / 3 / 3 / 3 / 3 / 3 / 3 / 3
Na / 3 / 3 / 1 / 2 / 1 / 3 / 1 / 1 / 2 / 2 / 1 / 3
Ho / 1.000 / 1.000 / 0.000 / 1.000 / 0.000 / 1.000 / 0.000 / 0.000 / 1.000 / 0.667 / 0.000 / 1.000
He / 0.667 / 0.667 / 0.000 / 0.500 / 0.000 / 0.611 / 0.000 / 0.000 / 0.500 / 0.444 / 0.000 / 0.611
7. Sidi Ben Ali 1 / N / 2 / 2 / 2 / 2 / 1 / 2 / 2 / 2 / 2 / 2 / 2 / 2
Na / 3 / 3 / 1 / 3 / 1 / 4 / 2 / 1 / 2 / 3 / 1 / 2
Ho / 1.000 / 1.000 / 0.000 / 1.000 / 0.000 / 1.000 / 0.000 / 0.000 / 0.500 / 1.000 / 0.000 / 0.500
He / 0.625 / 0.625 / 0.000 / 0.625 / 0.000 / 0.750 / 0.500 / 0.000 / 0.375 / 0.625 / 0.000 / 0.375
8. Sidi Ben Ali 2 / N / 2 / 2 / 2 / 2 / 1 / 2 / 1 / 2 / 2 / 2 / 2 / 2
Na / 2 / 3 / 1 / 2 / 1 / 4 / 1 / 1 / 2 / 2 / 1 / 2
Ho / 0.500 / 1.000 / 0.000 / 1.000 / 0.000 / 1.000 / 0.000 / 0.000 / 1.000 / 1.000 / 0.000 / 1.000
He / 0.375 / 0.625 / 0.000 / 0.500 / 0.000 / 0.750 / 0.000 / 0.000 / 0.500 / 0.500 / 0.000 / 0.500
9. Azzla / N / 22 / 22 / 22 / 22 / 15 / 22 / 21 / 21 / 22 / 22 / 22 / 22
Na / 12 / 6 / 1 / 10 / 11 / 8 / 3 / 2 / 3 / 4 / 2 / 9
Ho / 0.909 / 0.773 / 0.000 / 0.909 / 0.267 / 0.818 / 0.429 / 0.048 / 0.409 / 0.682 / 0.000 / 0.455
He / 0.863 / 0.768 / 0.000 / 0.805 / 0.889 / 0.808 / 0.595 / 0.459 / 0.429 / 0.723 / 0.087 / 0.489
10. Lac Fatzara / N / 2 / 2 / 2 / 2 / 2 / 2 / 2 / 2 / 2 / 2 / 2 / 2
Na / 3 / 3 / 1 / 4 / 2 / 4 / 2 / 2 / 3 / 1 / 1 / 3
Ho / 1.000 / 1.000 / 0.000 / 1.000 / 0.000 / 1.000 / 0.500 / 0.000 / 0.500 / 0.000 / 0.000 / 1.000
He / 0.625 / 0.625 / 0.000 / 0.750 / 0.500 / 0.750 / 0.375 / 0.500 / 0.625 / 0.000 / 0.000 / 0.625
11. Draa Elrich 3 / N / 4 / 4 / 4 / 4 / 3 / 4 / 3 / 4 / 4 / 4 / 4 / 4
Na / 6 / 5 / 1 / 5 / 3 / 7 / 1 / 2 / 3 / 3 / 1 / 3
Ho / 1.000 / 1.000 / 0.000 / 1.000 / 0.333 / 1.000 / 0.000 / 0.000 / 0.750 / 0.750 / 0.000 / 0.500
He / 0.813 / 0.750 / 0.000 / 0.750 / 0.611 / 0.844 / 0.000 / 0.375 / 0.625 / 0.531 / 0.000 / 0.531
12. Chetaibi 2 / N / 4 / 4 / 4 / 4 / 4 / 4 / 4 / 4 / 4 / 4 / 4 / 4
Na / 3 / 3 / 1 / 4 / 2 / 3 / 2 / 2 / 2 / 2 / 2 / 1
Ho / 0.000 / 1.000 / 0.000 / 0.500 / 0.000 / 0.750 / 0.000 / 0.000 / 0.250 / 0.500 / 0.000 / 0.000
He / 0.625 / 0.625 / 0.000 / 0.563 / 0.375 / 0.531 / 0.375 / 0.375 / 0.219 / 0.500 / 0.375 / 0.000
13. Draa Elrich 2 / N / 5 / 5 / 5 / 5 / 3 / 5 / 5 / 5 / 5 / 5 / 5 / 5
Na / 5 / 5 / 1 / 4 / 1 / 5 / 1 / 2 / 3 / 3 / 1 / 3
Ho / 1.000 / 1.000 / 0.000 / 1.000 / 0.000 / 1.000 / 0.000 / 0.200 / 0.800 / 0.600 / 0.000 / 0.800
He / 0.780 / 0.740 / 0.000 / 0.660 / 0.000 / 0.780 / 0.000 / 0.180 / 0.640 / 0.660 / 0.000 / 0.560
14. Draa Elrich 1 / N / 5 / 5 / 5 / 5 / 5 / 5 / 4 / 5 / 5 / 5 / 5 / 5
Na / 5 / 5 / 1 / 7 / 6 / 6 / 1 / 2 / 3 / 3 / 1 / 6
Ho / 0.800 / 0.600 / 0.000 / 0.600 / 0.400 / 1.000 / 0.000 / 0.200 / 0.800 / 0.400 / 0.000 / 0.800
He / 0.720 / 0.740 / 0.000 / 0.840 / 0.800 / 0.820 / 0.000 / 0.420 / 0.560 / 0.640 / 0.000 / 0.780
15. El Hashassia / N / 4 / 4 / 4 / 4 / 4 / 4 / 4 / 4 / 4 / 4 / 4 / 4
Na / 6 / 6 / 1 / 6 / 5 / 5 / 1 / 2 / 5 / 3 / 1 / 7
Ho / 1.000 / 1.000 / 0.000 / 1.000 / 0.250 / 1.000 / 0.000 / 0.000 / 0.750 / 0.250 / 0.000 / 1.000
He / 0.813 / 0.813 / 0.000 / 0.813 / 0.781 / 0.750 / 0.000 / 0.500 / 0.750 / 0.656 / 0.000 / 0.844
16. Aïn Barbar 3 / N / 2 / 2 / 2 / 2 / 2 / 2 / 2 / 2 / 2 / 2 / 2 / 2
Na / 2 / 4 / 1 / 4 / 4 / 3 / 1 / 2 / 2 / 3 / 1 / 3
Ho / 1.000 / 1.000 / 0.000 / 1.000 / 1.000 / 1.000 / 0.000 / 0.000 / 0.500 / 1.000 / 0.000 / 1.000
He / 0.500 / 0.750 / 0.000 / 0.750 / 0.750 / 0.625 / 0.000 / 0.500 / 0.375 / 0.625 / 0.000 / 0.625
17. Cross to Chetaibi / N / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1
Na / 2 / 2 / 1 / 2 / 1 / 2 / 1 / 1 / 1 / 2 / 1 / 1
Ho / 1.000 / 1.000 / 0.000 / 1.000 / 0.000 / 1.000 / 0.000 / 0.000 / 0.000 / 1.000 / 0.000 / 0.000
He / 0.500 / 0.500 / 0.000 / 0.500 / 0.000 / 0.500 / 0.000 / 0.000 / 0.000 / 0.500 / 0.000 / 0.000
18. Aïn Barbar 2 / N / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1
Na / 1 / 2 / 1 / 2 / 2 / 1 / 1 / 2 / 1 / 2 / 1 / 2
Ho / 0.000 / 1.000 / 0.000 / 1.000 / 1.000 / 0.000 / 0.000 / 1.000 / 0.000 / 1.000 / 0.000 / 1.000
He / 0.000 / 0.500 / 0.000 / 0.500 / 0.500 / 0.000 / 0.000 / 0.500 / 0.000 / 0.500 / 0.000 / 0.500
19. Oued Zied 1+2 / N / 8 / 8 / 8 / 8 / 7 / 8 / 5 / 8 / 8 / 8 / 8 / 8
Na / 8 / 6 / 1 / 10 / 7 / 9 / 3 / 2 / 6 / 3 / 1 / 7
Ho / 1.000 / 0.875 / 0.000 / 0.875 / 0.143 / 0.875 / 0.400 / 0.125 / 0.875 / 0.250 / 0.000 / 1.000
He / 0.844 / 0.813 / 0.000 / 0.867 / 0.827 / 0.859 / 0.340 / 0.492 / 0.797 / 0.594 / 0.000 / 0.844
20. Aïn Sayed / N / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1
Na / 2 / 2 / 1 / 2 / 1 / 2 / 1 / 2 / 2 / 2 / 1 / 2
Ho / 1.000 / 1.000 / 0.000 / 1.000 / 0.000 / 1.000 / 0.000 / 1.000 / 1.000 / 1.000 / 0.000 / 1.000
He / 0.500 / 0.500 / 0.000 / 0.500 / 0.000 / 0.500 / 0.000 / 0.500 / 0.500 / 0.500 / 0.000 / 0.500
22. Bousedra / N / 6 / 6 / 6 / 6 / 5 / 6 / 4 / 6 / 6 / 6 / 6 / 6
Na / 8 / 7 / 1 / 10 / 5 / 7 / 1 / 2 / 5 / 3 / 1 / 6
Ho / 1.000 / 1.000 / 0.000 / 1.000 / 0.200 / 0.833 / 0.000 / 0.167 / 0.667 / 0.333 / 0.000 / 1.000
He / 0.778 / 0.847 / 0.000 / 0.889 / 0.780 / 0.819 / 0.000 / 0.486 / 0.667 / 0.403 / 0.000 / 0.778
24. Lac d'Oiseaux / N / 4 / 4 / 4 / 4 / 4 / 4 / 3 / 4 / 4 / 4 / 4 / 4
Na / 5 / 3 / 2 / 5 / 4 / 6 / 1 / 2 / 4 / 4 / 1 / 6
Ho / 1.000 / 0.750 / 0.500 / 1.000 / 0.500 / 1.000 / 0.000 / 0.250 / 1.000 / 0.750 / 0.000 / 1.000
He / 0.781 / 0.656 / 0.375 / 0.750 / 0.750 / 0.813 / 0.000 / 0.219 / 0.656 / 0.656 / 0.000 / 0.813
25. Griat / N / 1 / 1 / 1 / 1 / 1 / 1 / 0 / 1 / 1 / 1 / 1 / 1
Na / 2 / 2 / 1 / 2 / 2 / 2 / 0 / 2 / 1 / 1 / 1 / 1
Ho / 1.000 / 1.000 / 0.000 / 1.000 / 1.000 / 1.000 / 0.000 / 1.000 / 0.000 / 0.000 / 0.000 / 0.000
He / 0.500 / 0.500 / 0.000 / 0.500 / 0.500 / 0.500 / 0.000 / 0.500 / 0.000 / 0.000 / 0.000 / 0.000
26. Berrihane / N / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1
Na / 2 / 2 / 1 / 1 / 2 / 2 / 2 / 1 / 2 / 2 / 1 / 1
Ho / 1.000 / 1.000 / 0.000 / 0.000 / 1.000 / 1.000 / 1.000 / 0.000 / 1.000 / 1.000 / 0.000 / 0.000
He / 0.500 / 0.500 / 0.000 / 0.000 / 0.500 / 0.500 / 0.500 / 0.000 / 0.500 / 0.500 / 0.000 / 0.000
28. Aïn Soltane / N / 2 / 2 / 2 / 2 / 2 / 2 / 2 / 2 / 2 / 2 / 2 / 2
Na / 4 / 4 / 1 / 4 / 3 / 3 / 1 / 2 / 3 / 2 / 2 / 2
Ho / 1.000 / 1.000 / 0.000 / 1.000 / 0.500 / 1.000 / 0.000 / 0.500 / 0.500 / 0.500 / 0.500 / 1.000
He / 0.750 / 0.750 / 0.000 / 0.750 / 0.625 / 0.625 / 0.000 / 0.375 / 0.625 / 0.375 / 0.375 / 0.500
29. Aïn Al-Assel / N / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1
Na / 1 / 2 / 1 / 1 / 1 / 2 / 1 / 1 / 1 / 2 / 1 / 1
Ho / 0.000 / 1.000 / 0.000 / 0.000 / 0.000 / 1.000 / 0.000 / 0.000 / 0.000 / 1.000 / 0.000 / 0.000
He / 0.000 / 0.500 / 0.000 / 0.000 / 0.000 / 0.500 / 0.000 / 0.000 / 0.000 / 0.500 / 0.000 / 0.000
30. Edkhailia 2 / N / 14 / 14 / 14 / 14 / 14 / 14 / 14 / 14 / 14 / 14 / 14 / 14
Na / 9 / 7 / 2 / 8 / 11 / 7 / 2 / 4 / 4 / 4 / 2 / 2
Ho / 0.786 / 1.000 / 0.571 / 0.929 / 0.857 / 0.857 / 0.143 / 0.429 / 0.643 / 0.714 / 0.071 / 0.214
He / 0.788 / 0.824 / 0.408 / 0.837 / 0.814 / 0.816 / 0.133 / 0.518 / 0.630 / 0.661 / 0.069 / 0.436
31. Laayoun / N / 3 / 3 / 3 / 3 / 3 / 3 / 3 / 3 / 3 / 3 / 3 / 3
Na / 3 / 5 / 1 / 4 / 6 / 3 / 2 / 3 / 3 / 2 / 2 / 2
Ho / 0.667 / 1.000 / 0.000 / 0.667 / 1.000 / 0.667 / 0.333 / 1.000 / 0.667 / 0.333 / 0.000 / 0.333
He / 0.611 / 0.778 / 0.000 / 0.667 / 0.833 / 0.500 / 0.500 / 0.667 / 0.611 / 0.278 / 0.444 / 0.278
32. Bouzzaroua / N / 3 / 3 / 3 / 3 / 3 / 3 / 3 / 3 / 3 / 3 / 3 / 3
Na / 4 / 3 / 1 / 3 / 6 / 6 / 1 / 2 / 2 / 2 / 1 / 3
Ho / 0.667 / 1.000 / 0.000 / 1.000 / 1.000 / 1.000 / 0.000 / 0.333 / 0.333 / 0.667 / 0.000 / 0.333
He / 0.667 / 0.611 / 0.000 / 0.611 / 0.833 / 0.833 / 0.000 / 0.278 / 0.278 / 0.444 / 0.000 / 0.611
33. Sandaj-Tabarka / N / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1
Na / 2 / 2 / 1 / 2 / 2 / 2 / 1 / 2 / 2 / 1 / 1 / 2
Ho / 1.000 / 1.000 / 0.000 / 1.000 / 1.000 / 1.000 / 0.000 / 1.000 / 1.000 / 0.000 / 0.000 / 1.000
He / 0.500 / 0.500 / 0.000 / 0.500 / 0.500 / 0.500 / 0.000 / 0.500 / 0.500 / 0.000 / 0.000 / 0.500
34. Erbayhia 1 / N / 4 / 4 / 4 / 4 / 4 / 4 / 4 / 4 / 4 / 4 / 4 / 4
Na / 4 / 3 / 1 / 4 / 7 / 6 / 2 / 1 / 3 / 2 / 1 / 2
Ho / 1.000 / 0.750 / 0.000 / 0.750 / 1.000 / 1.000 / 0.250 / 0.000 / 0.500 / 0.250 / 0.000 / 0.500
He / 0.688 / 0.656 / 0.000 / 0.719 / 0.844 / 0.781 / 0.219 / 0.000 / 0.594 / 0.219 / 0.000 / 0.375
35. Al-Haniya / N / 5 / 5 / 5 / 5 / 5 / 5 / 5 / 5 / 5 / 5 / 5 / 5
Na / 4 / 3 / 1 / 3 / 6 / 7 / 2 / 2 / 2 / 3 / 1 / 2
Ho / 0.800 / 0.600 / 0.000 / 0.400 / 1.000 / 1.000 / 0.400 / 0.600 / 0.400 / 0.800 / 0.000 / 0.600
He / 0.720 / 0.660 / 0.000 / 0.560 / 0.820 / 0.840 / 0.320 / 0.420 / 0.320 / 0.640 / 0.000 / 0.500
36. Al-Maajen / N / 4 / 4 / 4 / 4 / 4 / 4 / 4 / 4 / 4 / 4 / 4 / 4
Na / 5 / 3 / 1 / 6 / 7 / 8 / 2 / 3 / 4 / 2 / 1 / 2
Ho / 0.750 / 0.750 / 0.000 / 1.000 / 0.750 / 1.000 / 0.250 / 0.500 / 0.500 / 0.250 / 0.000 / 0.250
He / 0.750 / 0.531 / 0.000 / 0.813 / 0.844 / 0.875 / 0.219 / 0.594 / 0.563 / 0.219 / 0.000 / 0.219
37. Bessetine / N / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1
Na / 1 / 2 / 1 / 2 / 2 / 1 / 1 / 2 / 2 / 1 / 1 / 2
Ho / 0.000 / 1.000 / 0.000 / 1.000 / 1.000 / 0.000 / 0.000 / 1.000 / 1.000 / 0.000 / 0.000 / 1.000
He / 0.000 / 0.500 / 0.000 / 0.500 / 0.500 / 0.000 / 0.000 / 0.500 / 0.500 / 0.000 / 0.000 / 0.500
38. Ksar Chikh + Bousselem / N / 9 / 9 / 9 / 9 / 9 / 9 / 9 / 9 / 9 / 9 / 9 / 9
Na / 7 / 7 / 2 / 8 / 14 / 11 / 2 / 3 / 3 / 5 / 1 / 2
Ho / 0.556 / 0.667 / 0.111 / 0.778 / 1.000 / 1.000 / 0.222 / 0.111 / 0.778 / 0.556 / 0.000 / 0.444
He / 0.790 / 0.833 / 0.278 / 0.802 / 0.920 / 0.877 / 0.198 / 0.438 / 0.512 / 0.580 / 0.000 / 0.494
39. Tebraska / N / 4 / 4 / 4 / 4 / 4 / 4 / 4 / 4 / 4 / 4 / 4 / 4
Na / 3 / 4 / 1 / 5 / 5 / 6 / 1 / 4 / 1 / 2 / 1 / 2
Ho / 0.500 / 0.750 / 0.000 / 1.000 / 0.750 / 0.750 / 0.000 / 0.500 / 0.000 / 0.500 / 0.000 / 0.500
He / 0.656 / 0.719 / 0.000 / 0.781 / 0.750 / 0.781 / 0.000 / 0.750 / 0.000 / 0.375 / 0.000 / 0.375
40. Bouhdid / N / 5 / 5 / 5 / 5 / 5 / 5 / 5 / 5 / 5 / 5 / 5 / 5
Na / 2 / 3 / 1 / 3 / 4 / 7 / 2 / 3 / 1 / 1 / 1 / 2
Ho / 0.200 / 0.600 / 0.000 / 0.800 / 0.600 / 1.000 / 0.200 / 0.400 / 0.000 / 0.000 / 0.000 / 0.200
He / 0.180 / 0.460 / 0.000 / 0.540 / 0.480 / 0.840 / 0.180 / 0.460 / 0.000 / 0.000 / 0.000 / 0.180
41. Bizerte 4+5 / N / 2 / 2 / 2 / 2 / 2 / 2 / 2 / 2 / 2 / 2 / 2 / 2
Na / 2 / 4 / 1 / 3 / 3 / 2 / 1 / 3 / 1 / 3 / 1 / 2
Ho / 0.500 / 1.000 / 0.000 / 1.000 / 1.000 / 0.500 / 0.000 / 1.000 / 0.000 / 0.500 / 0.000 / 1.000
He / 0.375 / 0.750 / 0.000 / 0.625 / 0.625 / 0.375 / 0.000 / 0.625 / 0.000 / 0.625 / 0.000 / 0.500
42. Tinja / N / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1
Na / 2 / 2 / 1 / 2 / 1 / 2 / 1 / 2 / 1 / 1 / 1 / 2
Ho / 1.000 / 1.000 / 0.000 / 1.000 / 0.000 / 1.000 / 0.000 / 1.000 / 0.000 / 0.000 / 0.000 / 1.000
He / 0.500 / 0.500 / 0.000 / 0.500 / 0.000 / 0.500 / 0.000 / 0.500 / 0.000 / 0.000 / 0.000 / 0.500
43. Bizerte 1 / N / 4 / 4 / 4 / 4 / 4 / 4 / 4 / 4 / 4 / 4 / 4 / 4
Na / 3 / 4 / 1 / 4 / 7 / 5 / 1 / 1 / 1 / 1 / 1 / 2
Ho / 0.750 / 0.500 / 0.000 / 1.000 / 1.000 / 1.000 / 0.000 / 0.000 / 0.000 / 0.000 / 0.000 / 0.250
He / 0.656 / 0.563 / 0.000 / 0.656 / 0.844 / 0.750 / 0.000 / 0.000 / 0.000 / 0.000 / 0.000 / 0.219
44. 13 km Menzel Bouzelfa / N / 11 / 11 / 11 / 11 / 11 / 11 / 11 / 11 / 11 / 11 / 11 / 11
Na / 3 / 8 / 1 / 6 / 8 / 6 / 1 / 3 / 1 / 2 / 2 / 3
Ho / 0.818 / 0.909 / 0.000 / 0.727 / 0.909 / 1.000 / 0.000 / 0.545 / 0.000 / 0.091 / 0.000 / 0.364
He / 0.628 / 0.847 / 0.000 / 0.744 / 0.814 / 0.748 / 0.000 / 0.525 / 0.000 / 0.087 / 0.165 / 0.595
45. Oued El Mida / N / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1
Na / 2 / 2 / 1 / 1 / 2 / 2 / 1 / 2 / 2 / 1 / 1 / 1
Ho / 1.000 / 1.000 / 0.000 / 0.000 / 1.000 / 1.000 / 0.000 / 1.000 / 1.000 / 0.000 / 0.000 / 0.000
He / 0.500 / 0.500 / 0.000 / 0.000 / 0.500 / 0.500 / 0.000 / 0.500 / 0.500 / 0.000 / 0.000 / 0.000

Supplementary Figure 1. Delta K plot (top) and graph plotting the log probability of the data (ln Pr(X|K) across the range of K values tested (bottom); in addition to Evanno’s method, the optimal K value can be selected based on where the value of ln Pr(X|K) plateaus and variance is minimized. Either way, K = 2 and K = 3 are shown to be the best descriptors of hierarchical patterns of genetic structure.