Supplementary Fig. 1. Map showing the trapping sites for the rodents in Jiangxia district ,Hubei province, China. Samples were viral RNA-positive in HJC (JX1143,JX0937,JX1018 and JX1028) and ZJW (HV003,HV004 and HV005).
Supplementary Tabel 1. Primers used for sequencing the whole genome of hantavirus strain HV004
Segment / Forward primer(5'--3') / Reverse primer(5'--3') / Expected size(bp)S / S1F
AGGGTCAACTGGTGATAGCC / S1R
CTTTGACGCCGTAATGGTAT / 995
S2F
GCTCTGGCAAAAGATTGGAGTG / S2R
CAAGGAGCAAGCAAATGAGG / 938
S3F
ATCCATTGTGGGTGGGTTTAG / S3R
TTTCCGGTTGCAATCCTATC / 479
M / M1F
ACACCGCAAAAGAAAGCAGT / M1R
ATTGAATGTGCTACCCCAGG / 1440
M2F
AAAGCAGAATCGTTTTAGAC / M2R
ATTGGTAATCCCTTTCATAG / 1052
M3F
ACACCTACCGAAGGAAACTAAC / M3R
ACAGAGGACAATGAGGACAATC / 1285
M4F
AACACAGTCAAAGTCTCAGGGA / M4R
ATCGGAGGCAGTTCAACATATC / 680
L / L1F
TAGTAGTAGACTCCCTAAAGAGCT / L1R
TCTAAAACCGTATCAACATAAGTG / 1392
L2F
GCATGTTAAGGGCAGAGTCAAAGGC / L2R
TGCCCATCTTCTTGAACTTGTCCTG / 1399
L3F
ACGGTTACATGATGTGGGAACTTCG / L3R
ACACCTGCTCTGTGCTGCTGC / 1340
L4F
ACAGTATCCCCAACCAATGCTGA / L4R
TCAAGTCTAGCACAATCGAGACCA / 1742
L5F
CAGAATGGCTAGATAGATTTTGTA / L5R
TAGTAGTAGTATGCTCCGGAAAAT / 1515
L6F
TCTTTCTATTAGGCTTTCTTTC / L6R
TTTGTAATTATCAGGTGTCATC / 431
Supplementary Table 2. Hantavirus strains used for analysis in this study
Type / Virus strain / Source / Country / GenBank accession No.S / M / L
HTNV / HV003 / A. agrarius / Hubei, China / JQ083396 / - / -
HV004 / A. agrarius / Hubei, China / JQ083395 / JQ083394 / JQ083393
HV005 / A. agrarius / Hubei, China / JQ083397 / - / -
s200903 / Human / Hubei, China / JQ083402 / - / -
s201012 / Human / Hubei, China / JQ083403 / - / -
CGAa4P15 / A. agrarius / Guizhou, China / EF990914 / EF990928 / -
CGAa4MP9 / A. agrarius / Guizhou, China / EF990915 / EF990929 / -
CGAa1011 / A. agrarius / Guizhou, China / EF990913 / EF990927 / -
LR1 / Vaccine / China / AF288294 / AF288293 / AF288292
76-118 / A. agrarius / Korea / M14626 / M14627 / X55901
lee / Human / Korea / - / D00377 / -
Maaji-2 / Not known / Korea / AF321095 / - / -
CJAp93 / A. peninsulae / Jilin,China / EF208929 / EF208930 / -
Bao14 / A. agrarius / Heilongjiang, China / AB027105 / AB027068 / -
ZLS-12 / A. agrarius / Zhejiang, China / FJ753398 / FJ753399 / -
ZLS6-11 / A. agrarius / Zhejiang, China / FJ753396 / FJ753397 / -
TJJ16 / N. Confucianus / Tianjin, China / AY839871 / EU074672 / -
A16 / A. agrarius / Shaanxi, China / AF288646 / - / -
Q32 / A. agrarius / Guizhou, China / AB027097 / AB027061 / DQ371906
CGRni1 / R. nitidus / Guizhou, China / EU363812 / EU363815 / -
CGRn45 / R. norvegicus / Guizhou, China / EU092221 / EU092225 / -
KY / White rats / Yunnan, China / GU140098 / GU140097 / GU140096
Chen4 / Human / Anhui, China / AB027101 / - / -
E142 / E. eleusis / Yunnan, China / AF288644 / - / -
84FLi / Human / Shaanxi, China / AY017064 / AF345636 / AF336826
SN7 / N. confucianus / Sichuan, China / AF288657 / AF288656 / -
Z10 / Human / Zhejiang, China / AF184987 / AF276987 / AF189155
Z5 / Not known / Zhejiang, China / EF103195 / EU074224 / -
Z251 / A. agrarius / Zhejiang, China / EF595840 / - / -
Hu / Human / Hubei, China / AB027111 / - / -
A9 / A. agrarius / Jiangsu, China / AF329390 / AF035831 / AF293665
HV114 / Human / Hubei, China / - / ML08753 / -
CGRn8316 / R. norvegicus / Guizhou, China / EF990903 / EF990917 / -
CGRn9415 / R. norvegicus / Guizhou, China / EF990902 / EF990916 / -
Cumc-B11 / Not known / Korea / U37768 / U38177 / -
HTN261 / A. agrarius / Hainan, China / AF252259 / - / -
CGHu1 / Human / Guizhou, China / EU092218 / EU092222 / -
CGAa31p9 / A. agrarius / Guizhou, China / EF990910 / EF990924 / -
CGHu2 / Human / Guizhou, China / EU363813 / EU363819 / -
CGHu3612 / Human / Guizhou, China / EF990909 / EF990923 / EF990937
CGAa31MP7 / A. agrarius / Guizhou, China / EF990911 / EF990925
SC-2 / A. peninsulae / Korea / AY675350 / DQ056293 / AY675354
SC-1 / A. peninsulae / Korea / AY675349 / AY675353 / DQ056292
DBSV / AH09 / Not known / Anhui, China / AF285264 / - / -
NC167 / N. confucianus / Anhui, China / AB027523 / - / -
SEOV / JX1018 / R. norvegicus / Hubei, China / JQ083398 / - / -
JX1028 / R. norvegicus / Hubei, China / JQ083399 / - / -
JX1037 / R. norvegicus / Hubei, China / JQ083400 / - / -
JX1037 / R. norvegicus / Hubei, China / JQ083401 / - / -
ZT10 / A. peninsulae / Zhejiang,China / AY766368 / DQ159911 / -
Z37 / R. norvegicus / Zhejiang,China / AF187082 / AF190119 / -
Tchoupitoulas / R. norvegicus / USA / AF329389 / - / -
80-39 / R. norvegicus / Korea / - / - / X56492
BjHD01 / R. norvegicus / Beijing, China / AY627049 / DQ133505 / -
SR11 / R. norvegicus / Japan / M34881 / M34882 / -
DOBV / Sk/Aa / A. agrarius / Slovakia / AY961615 / AY961616 / GU904039
PUUV / Sotkamo / C. glareolus / Finland / NC005224 / NC005223 / NC005225
SNV / NM11 / Human / USA / NC005216 / - / -
Note:“-”presents the sequence is unavailable in GenBank or not be used in this study. Abbreviations: HTNV, Hantaan virus; DBSV, Da Bie Shan virus; SEOV, Seoul virus; DOBV, Dobrava-Belgrade virus; PUUV, Puumala virus; SNV, Sin Nombre virus. A. agrarius, Apodemus agrarius; A. peninsulae, Apodemus, peninsulae; N confucianus ,Niviventer confucianus; R. nitidus, Rattus nitidus; R. norvegicus, Rattus norvegicus; E. eleusis, Eothenomys Eleusis; C. glareolus, Clethrionomys glareolus.