Supplementary Material Figure S1

Supplementary Material Figure S1

Supplementary Material Figure S2

Dietary component
(% Weight) / Chow
Control / High-Fat High-Sucrose
(HFD)
Protein (%) / 20.00 / 19.36
Carbohydrate / 59.40 / 25.6
Total fat (%)
Crude Fibre (%)
AD Fibre (%)
Digestible Energy
(MJ/kg)
% Total digestible energy
from lipids
% Total digestible energy
from protein
Calculated Fatty Acid Composition (%)
Saturated fats C12 or less
Myristic Acid 14:0
Palmitic Acid 16:0
Stearic Acid 16:0
Palmitoleic Acid
Oleic Acid 18:1
Gadoleic Acid 20:1
Linoleic Acid 18:2 n6
α Linoleic Acid 18:3 n3
Stearidonic Acid 18:4 n3
Arachidonic Acid 20:4
Eicosapentanoeic Acid 20:5 n3
DHA 22:6 n3
Total n3
Total n6
Total Saturated Fats
Total monounsaturated fats
Total polyunsaturated fat
Cholesterol / 4.80
4.80
7.60
14.0
23.00
12.00
No data
0.03
0.50
0.14
0.01
1.90
0.03
1.30
0.30
No data
0.01
0.02
0.05
0.37
1.31
0.74
2.00
1.77
No data / 23.00
4.66
4.66
20.0
43.00
17.00
6.76
1.80
3.15
3.05
No data
5.92
0.10
1.37
0.56
0.03
None
Trace
No data
0.60
1.40
14.90
6.10
2.00
0.19
Casein (Acid)
Sucrose
Canola Oil
Cocoa Butter
Hydrogenated
vegetable oil
Corn Oil
Cellulose
Wheat starch
Dextrinised starch
Choline Chloride
AIN Vitamins / No data
No data
Trace
No data
No data
No data
No data
No data
No data
No data
No data / 200
405
50
50
131
-
50
50
0
2.5
10

Supplementary Material Table S3

Genes / Forward (5’3’) / Reverse (5’3’)
Btf3
Srebp-1c
Fasn
Scd 1
Lfabp
Ppar-α
Cpt1A
Cd36
Ppar-γ
AdipoR2
Col1a1
Tgf-β1
α – Sma
Timp-1
Tnf-α
Mmp-2
Bax
Bcl-2
Tlr2
Tlr4
Tlr5
Tlr7
Mcp-1
Hgf
Grp94
Adh1
Aldh2 / TGGCAGCAAACACCTTCACC
CACAGCAACCAGAAGCTCAA
TACCAAGCCAAGCACATTCG
CCTCTTCGGGATTTTCTACTACATG
AAGTCAAGCCAGTCGTCAAGCT
CATGTGAAGGCTGTAAGGGCTT
AGACCGTGAGGAACTCAAACCTA
ACAGACGCAGCCTCCTTTC
CATTGAGTGCCGAGTCTGTG
TACACACAGAGACGGGCAAC
ACTGGTACATCAGCCCGAAC
ACAGCCCTCGCACCCA
ACAGCCCTCGCACCCA
TCCTCTTGTTGCTATCACTGATAGCTT
GCAGGTTCTGTCCCTTTCAC
CCGAGGACTATGACCGGGATAA
TTGCTACAGGGTTTCATCCA
CTCGTCGCTACCGTCGTGACTT
GCGCAAGATAATGAACACCA
CTGGGGAGGCACATCTTCT
CTCAACACCTGGATGCTCA
TTCTATGGAGAGCCGGTGATA
ATCCCAATGAGTAGGCTGGA
TGCTCCTCCCTTCCCTATC
GTCGGGAAGCAACAGAGAAG
GTTGGAGAAGGGGTGACTTG
GCCTCAGGTGGATGAAACTC / AGCTTCAGCCAGTCTCCTTAAAC
TCATGCCCTCCATAGACACA
TGGCTTCGGCATGAGA
GCCGTGCCTTGTAAGTTCTGT
TGAGTTCAGTCACGGACTTTATG
TCTTGCAGCTCCGATCACACT
TGAAGAGTCGCTCCCACT
CAGATCCGAACACAGCGTAG
GCTTCAATCGGATGGTTCTT
TGGCTCCCAAGAAGAACAAG
TACTCGAACGGGAATCCATC
GCCACCGATCCAGACAGAGT
GCCACCGATCCAGACAGAGT
CGCTGGTATAAGGTGGTCTCGTT
AGTGCCTCTTCTGCAGTTC
CTTGTTGCCCAGGAAAGTAAG
GGAGACACTCGCTCAGCTTC
CAGATGCCCGGTTCAGGTACTCAGTC
GTCAGGAACTGGGTGGAGAA
CCAAGTTGCCGTTTCTTGTT
CCCGCAATGAAGTCTCTTTCT
TCTTTAGATTTGGCGGCATAC
TCTGGACCCATTCCTTCTTG
AAGTTTGGTCCCCCACATC
CCACAAGAGCACAGGGAGAC
AGATCGCTTCGGCTACAAAA
CGGTGGGCTGGATAAGTAG

Supplementary Table S4

The following primary antibodies were obtained from Cell Signalling Technologies: ADH1, ALDH1A1, Phospho-AMPK, AMPKα, phospho-ERK I/2, ERK1/2, phospho-JNK, JNK/SAPK, PERK, phosphor-EIF2a, EIF-2a, CHOP,GRP78/Bip, Atg3, Atg7, Atg12, IRE-1α, Beclin-1 LC3A, LC3B and β-actin. Phospho-IRE1α was obtained from Thermofisher Scientific. Anti-CYP2E1 antibody was purchased from Sigma Aldrich (St. Louis, MO, USA) and anti-4-hydroxynonenal (4-HNE) antibody from Cell Biolabs (Jomar Bioscience, Adelaide, Australia). Primary and secondary antibodies were diluted in 10% skim milk with Tris-buffered saline and 0.1% Tween. Secondary antibodies were either a goat anti-rabbit IgG or a rabbit anti-mouse IgG (Invitrogen, Australia).

Supplemental Figure S5

Supplemental Figure S6

SUPPLEMENTARY MATERIAL FIGURE LEGENDS

Supplementary Material Figure S1. Schema of animal feeding regime

Supplementary Material Figure S2. Macro- and Micronutrient Composition of Pellet Experimental Diets

Supplementary Material Table S3. Mouse primer sequences for genes used in RT-PCR

Supplementary Table S4. Antibodies used in Western Blot experiments

Supplemental Figure S5 RT-PCR analysis of genes involved in lipogenesis fatty acid synthase (Fasn) and stearoyl coA desaturase-1 (Scd1) in the liver

Supplemental Figure S6. Representative Western Blots and corresponding quantitative analysis of proteins involved in the apoptotic signalling pathways in the liver