1

Supplementary Information

Supplementary Table 1. Strains*

Strain / Genotype / Reference/Source
NCIB3610 / Wild-type
CA017 / tasA::kan
HV1150 / hag::tet
ZK2771 / PY79 sigF::kan / (Branda et al. 2001)
CA002 / sigF::kan
SSB488 / epsA-O::tet / (Branda et al. 2006)
DS73 /
cheA::tet
/ (Kearns and Losick 2003)
DS69 / cheY::tet / (Kearns and Losick 2003)
HV1048 / amyE::Phag-cfp (spec)
HV1049 / amyE::PsspB-yfp (spec)
HV1123 / amyE::Phag-yfp (spec)
CA018 / amyE::PyqxM-yfp (spec)
CA019 / amyE::PyqxM-cfp (spec)
HV1066 / amyE::Phag-cfp (spec) lacA::PsspB-yfp (mls)
HV1161 / amyE::PyqxM-cfp (spec) lacA::PsspB-yfp (mls)
RL2453 / PY79 amyE::PspoIIG-gfp (spec) / (Fujita and Losick 2002)
CA044 / amyE::PspoIIG-gfp (spec)
CA047 / tasA::kan amyE::PspoIIG-gfp (spec)
HV1174 / tasA::kan amyE::Phag-yfp (spec)
CA020 / tasA::kan amyE::PyqxM-yfp (spec)
CA021 / tasA::kan amyE::PyqxM-cfp (spec)
HV1176 / tasA::kan amyE::PsspB-yfp (spec)
HV1177 / sigF::kan amyE::Phag-yfp (spec)
HV1178 / sigF::kan amyE::PyqxM-yfp (spec)
HV1151 /
hag::tet amyE::Phag-cfp (spec)
HV1111 /
cheA::tet amyE::Phag-cfp (spec)
HV1112 / cheY::tet amyE::Phag-cfp (spec)

*Unless indicated, strains are derivatives of B. subtilis NCIB3160

Supplemental References

Branda, S.S., F. Chu, D.B. Kearns, R. Losick, and R. Kolter. 2006. A major protein component of the Bacillus subtilis biofilm matrix. Mol Microbiol 59: 1229-38.

Branda, S.S., J.E. Gonzalez-Pastor, S. Ben-Yehuda, R. Losick, and R. Kolter. 2001. Fruiting body formation by Bacillus subtilis. Proc Natl Acad Sci U S A 98: 11621-6.

Fujita, M. and R. Losick. 2002. An investigation into the compartmentalization of the sporulation transcription factor sigmaE in Bacillus subtilis. Mol Microbiol 43: 27-38.

Kearns, D.B. and R. Losick. 2003. Swarming motility in undomesticated Bacillus subtilis. Mol Microbiol 49: 581-90.