Supplementary Data Table S2

Supplementary data Table S2

Specific primers used for real-time RT-PCR. Primers were designed to amplify PCR products from 90 to 120 bp, a Tm around 62°C, and not to give any other unspecific match when blasted on the C. canephora EST unigene set. PCR efficiencies estimated for each primer pairs from the LinReg software (Ramakers et al. 2003) are indicated.

gene number / Genebank accession / Putative Function / primer forward / primer reverse / Product size / PCR efficiencies
1 / EH662404 / 4-coumarate-CoA ligase / CCTCTTCTGCCTTCCCATAATC / TTGTGTAGGAGCAAGTTCATCA / 112 / 1.845
2 / EH662405 / Ferulate-5-hydroxylase / AGTGAGGGCCAAGATGAGTTC / TCGAGCCAGTATAGCCAAGG / 101 / 1.896
3 / EE194658 / Beta-ketoacyl-ACP synthase II / CCCTTTCTTCTCTGGTGGTG / CTATGGCTACTGCTTTCCCAG / 101 / 1.919
4 / EE198092 / Beta-ketoacyl ACP synthase I / AACGCATGACAAAAGACACAGC / CGATGCAGATTGATGGACAG / 91 / 1.744
5 / EE196983 / Omega-6 fatty acid desaturase / CTTGCCTGGGTTGTCTGTG / TCGTAATGAGGCAACGAAGG / 107 / 1.985
6 / EE195565 / Acyl-CoA oxidase / ACTTGGTCACGGCTCTAACG / GCCACCACTTGCTTGAAGTC / 110 / 2.000
7 / EE198985 / P450 - ripening-related / TATTGGATCGCACTCACAGC / TGAAGAGGGAGTGAAACCAG / 113 / 1.660
8 / EE197233 / Laccase (diphenol oxidase) / CGTACATAACTCAGTGCCCC / GTCACTATGAGCATGCCACC / 101 / 1.866
9 / EE192027 / Polyphenol oxidase / TTGCAATAAGCGCAATGAAC / GCTGCTCATGTGGTCAAGAA / 128 / 1.891
10 / EE199918 / Lipid transfer protein / CTTCAAGAATAGCGCCCAAC / GCTCAATCCACAGTTGCTGA / 109 / 1.925
11 / EH662410 / Inositol-3-P-synthase / GGACAAAGTGCAACAAGCAA / GCTCTTGAATGGGGCATAGA / 103 / 1.964
12 / EH662409 / Nicotianamine Synthase / TCAAGCCCTCCAAAGATGTC / TTGGGCCTCATTTCTTGAAC / 115 / 2.000
13 / EE198324 / Glyoxal oxidase / GGATGCTAGATGGACCCACTG / CCCGGAAGATACAGGATTGG / 126 / 1.896
14 / EE193281 / Acyl-ACP thioesterase / CGCGAAAACTTCAGCATCAG / TCACCAAGCAGTCCAGCAGT / 125 / 1.601
15 / EE199078 / Enoyl-CoA hydratase / CCAGCAAGTCAAAGCCGAAT / TTGGAGGACATGAGCTGTGC / 132 / 1.992
16 / EE194810 / Omega-3 fatty acid desaturase / CAAAACCAAAAACTGGCCTGA / CAATGCCCAATTCCTGTCCT / 116 / 1.826
17 / EE200040 / 11S albumin / CAAGAGCAACAACCCCAACA / GGGAGGCCAATGTTTTCACT / 125 / 1.847
18 / AB039725 / Caffeine synthase / gtggccgaatgctccttact / acgtccctcagcaaccaag / 107 / 1.951
19 / AB048793 / Xanthosine N-methyltransferase / CAACTGGTTCTCGCCAAGGT / CATCCCAAATCCGCAACTTT / 110 / 1.962
20 / EE199922 / P450 - geraniol 10-hydroxylase / GGCCCTATTCGTTTCCAATC / CGGTTGCTTTGCTTCCTAGC / 126 / 1.743
21 / EE199971 / Chalcone isomerase / CCCCTAACCTTTGGCGTTTA / GGTTTTCCCGTTTCAGTGCT / 96 / 1.713
22 / EL645414 / Caffeoyl-CoA 3-O-methyltransferase CCoAOMT / GCCAAAATCTCAGGCATCAA / CTTTCATGGGCTCTGGCTCT / 114 / 1.949
23 / EE195971 / Cinnamyl-alcohol dehydrogenase / ACACCAACACTCCCCATTGC / TCCAAGCTCCCCATTTTCTG / 123 / 1.915
24 / EE200510 / Dehydrin dh3 / GGATCGCGGACTCTTCAACT / CCCTCCTCCTTGTCTGGTTC / 120 / 1.940
25 / DV711566 / Starch synthase / GCCATTCCCAAGCTCATTCA / TGTCGGGGTACATCACCTCA / 189 / 1.870
26 / EE195901 / Caleosin / ATCGAGAGAGATGGCATTGG / CAGTCCGAACATTCCTCTCC / 95 / 1.854
27 / EE200432 / Glutathione peroxidase / CTGAAAATATGGCTACCGCG / GCTAAGGGGCACATCATTTC / 89 / 1.928
28 / EE199490 / Sugar transporter family / TGGAGGAGGAAATTGATCAGC / CAGCAAGAAAAGCAAGCCTC / 117 / 2.000
29 / EE200223 / Fiber annexin / ATGTCCCGTCTGTTGCTGAA / CGGCATTCCTATGAGCCAAA / 105 / 1.959
30 / EE198189 / Basic 7S globulin isoform / GTTTGCATCAGCACCTGGAG / AGGAGGCTTGTGGCGTTATC / 90 / 1.843
31 / EE195736 / Dehydration-induced protein RD22-like protein / TTGTTCTCTGCTCGGAAGGTT / AGGCGCATTTGGGTTTAGAG / 118 / 1.968
32 / EE195879 / Expansin 2 / TGCCTACCGGGAACCATTAC / CAGGCTCTGCCAAATCAAAG / 121 / 1.830
33 / EE199914 / Imbibition protein - glycosyl hydrolase / TGGGATCTACAATGCCAAGC / TGAAATGTGTATGGTGTGGGAAG / 91 / 2.000
34 / EE195889 / Pectate lyase / GCACTACGGGTGGAGGACC / AGTTGGACAAGGAGCAATGG / 92 / 1.835
35 / EE196692 / Endo-1,4-beta-glucanase / CAAACCAAACCTCCCCTCTC / TTGCCATGAGTTCAATGTGG / 103 / 1.808
36 / EL610519 / Thaumatin-like protein / CCTACACTTTGTCCCTTTTGGC / AGCTGCCCAAACTGTGTAGGT / 107 / 1.821
37 / EH682411 / ABC transporter family protein / GTCGGAGAAAAAGGGGAGAG / CCATCCTCCGACTC / 62 / 1.866
38 / EE195857 / Catalase / TCCTCTTTGATGATGTCGGC / AGCTTTTCCAGCCTTGTCG / 94 / 1.994
39 / EE199954 / Copper/zinc-superoxide dismutase / CTCCCTCCCTCTCTCTTTCC / GCCACTCACACACTCATTGC / 97 / 2.000
40 / EE195613 / Superoxide dismutase [Mn], mitochondrial / ACACATTGCCTGATCTTCCC / CCTTGTTGAAATTGGTGACG / 116 / 1.911
41 / EE199671 / ACC oxidase / AAAAGTAGCCACCACAAAGGC / CCCAGTTTTCACAAGCATCC / 122 / 1.789
42 / EH662412 / ACC oxidase homologue / GCAAGATCGGTGCTTTTCC / TTTTCCATCATCCACCAGATG / 108 / 1.724
43 / EE199013 / Hexose carrier protein / GGGGATATGCGTTTGCACTT / ACTCGGCACCAACCAACCTA / 92 / 1.630
44 / EE197695 / 2S albumin / GGAGAATGGCAAAGCTCACA / TTGTCCTGGGTCCTCCTCAT / 128 / 1.790
45 / EL610525 / Hydroxycinnamoyl CoA quinate transferase / CCCACAAAGAGGCTATGGAA / TGAGCACACGGGTATCAAAG / 118 / 1.719
46 / EE199806 / Phenylalanine ammonia-lyase 3 / CGAGCAACACAATCAGGATG / CCTGACAAAGTGCCACTAGG / 117 / 1.797
47 / EE198074 / Phenylalanine ammonia-lyase 2 / GTTACGGAGTTACCACTGGG / GCCGTTTCCGAAGATTCC / 115 / 2.000
48 / AM116757 / Hydroxycinnamoyl transferase hct1 / CCCAGCCTCAATTCCAGC / GGACACCGCAGTTTCAGG / 101 / 1.730
49 / EH662413 / Secretory peroxidase (cationic) / AGGCAATCAAGCAACAACG / GGGAAGAAAGGGAGGATTG / 123 / 1.838
50 / EE196059 / Sorbitol dehydrogenase / TGCTACTCCGCCTGTTCATG / CCTCCAGGCTCACGTTATCAG / 92 / 1.996
51 / EE192973 / Sucrose synthase sus1 / ACTAGGCTGCTACCTGATGCC / AGATCCATTTGCGAACAACG / 130 / 1.983
52 / EE200220 / Invertase / CCAATGCTATGCTATCTTGGC / CAACCCATGTGGAACAATGG / 97 / 1.746
53 / EE196065 / Beta-galactosidase / TTGCCAAGGCATCCTAGAAC / TCTTCTCCCAAAACAAGACGTC / 110 / 1.913
54 / EE196011 / Stachyose synthase / TGGCAGGGATTTCCTCAGC / TTATCTCGGGCACATCCAAG / 122 / 1.703
55 / EE200469 / Arabinosidase ARA-1 / CTGGCACGGGTAGTCTTTTG / ATTCCACCTCCTGTCATTGG / 131 / 1.982
56 / EE198652 / Betaglucosidase - Xylosidase / ACCCGGAGTCCATTTTGG / GTTCCCACAAGGAAGCATTG / 95 / 1.936
57 / EE196432 / Sucrose phosphatase / TTAATGCCTTGTGGGAAGCC / AGGCTTCTCTTTCCGCAACTC / 101 / 1.939
58 / EE195851 / Dicarboxylate/tricarboxylate carrier / TTCGTCCTTCCCCTTTCTCT / CGTCTTAGGCTTCTCCTCTCC / 90 / 1.863
59 / EE200515 / Ethylene receptor 1 / AGAAGGGTGTGTAGACCAGGG / AACTCATCAGCAGGCCATTG / 98 / 1.711
60 / EE192447 / S-adenosylmethionine decarboxylase / GAATCAAGCCTCTTTGTTCATCC / GATTCAGCTAACTTCAGGATGGG / 101 / 2.000
61 / EE199454 / Pyruvate kinase / TTGACGTGTTTGATCGGACG / GGTCCCAAAGTGCAGACAATC / 112 / 2.000
62 / EE193293 / Lipoxygenase / TGCTAAAGCTCACGTTCTTGC / GCTAAGTTGCCTATTGGTTGC / 115 / 1.955
63 / EE192510 / Tocopherol cyclase / GCCCTTTCCTTGTTTGAATCC / AACTTGGGGAAGAACTGCTG / 89 / 1.781
64 / EE200298 / Thioredoxin peroxidase / CTCTTCCTTCACCAATCGCC / GATCAAAAACAGCCTCTGCC / 101 / 1.958
65 / EE199426 / Ascorbate peroxidase / TTCAACAGCGACGACACC / AGGTCATGAGGCCAACAATGG / 117 / 1.963
66 / EE192051 / Superoxide dismutase [Fe] / TCTGAGAAAGGCTGGTCCTC / CTTCCCCCAGTGGTATTCC / 121 / 2.000
67 / EH662415 / Cinnamic acid 4-hydroxylase / GGGACGATACCACCACAAGA / GAACCACCGCAACCTCACT / 92 / 1.956
68 / EE193134 / Polygalacturonase / CACAGATGGAATCAACCCAGA / CATACTCGTCCCACCCACTT / 107 / 1.784
69 / EE200222 / Aspartate transaminase / ATGGCGGGAGTTACCACAG / CGCTTGGATTGTTGCTGAAA / 112 / 1.755
70 / EL610518 / Cysteine synthase / TGTCCATCTTTCGCCCAGAG / TGGTGTTCTCCCAATCAGC / 96 / 2.000
71 / EE199889 / Glutamate dehydrogenase / CGCGGAGTCAAAGGATTCTG / CCAAGGGCTGCTGGAATAAG / 92 / 1.940
72 / EE197573 / Aconitase / AGGAGCAAGGAAAAGCAGCA / TGACGCTGGGGTTTGATGTA / 117 / 1.962
73 / AJ419827 / Rubisco (small subunit) / CCGTCCTCTTCCCCTCAAAT / CCTGAAAGTACAGCCCCAGTTC / 91 / 1.601
74 / EH662406 / PsbB photosystem II CP47 protein / CGGGTCAATACCAGCAAAAA / AGCAGTCCAAGGGGTTGGTT / 120 / 2.000
75 / EE199320 / Phosphate transporter / TCCCGAGTATGCCTCCAAAT / AAAGGGGCAAAGAGCAATGT / 91 / 1.883
76 / EH662414 / Cinnamoyl CoA reductase / GCTCAAGGATTTGGGGTTTG / GAGTGGGGAGGGGTATCTGG / 98 / 1.964
77 / EH662407 / Catalase / CGATTTGGTGGGAAACAATAC / CCTGGATGTGGGTCTTTGGA / 104 / 1.916
78 / EE192865 / Ferredoxin 2 / TCACAAGGAGGGCGAACTTT / CAGGACAAAACCCAAACATTCA / 93 / 2.000
79 / AF454631 / Caffeic acid O-methyltransferase / atcgcatcctccgactcct / gccagaccgtaaagcctctc / 94 / 2.000
80 / EE195803 / Esterase/lipase/thioesterase family / TCCGCAACACCAAGTCCTAC / GCCGTAACCGTGGCTCAT / 119 / 2.000
81 / EE200368 / ATP citrate lyase / GGCAAAGTTCGGGCTCCTAC / CACCAACACCAAATCCCTGT / 118 / 1.996
82 / EE199399 / Plastid Terminal oxidase / GGATGAACAGAAAGTGGTGGTG / GAAGCATCAGGTGGCTCTCC / 93 / 1.950
83 / EL610523 / Chalcone reductase / CCTTTGCCACCATCAGAACA / GGCCTCCTCTGTGCCATATAA / 102 / 2.000
84 / EE193656 / P-coumaroyl 3'-hydroxylase / ttcagagggtgtggttgatga / aacgaagccaaggaatgtga / 110 / 1.794
85 / EE197767 / Galactokinase / GGCACACAATCTGGTGGAAT / TGGCACGAATAGGGTTGAAA / 91 / 1.677
86 / EE199516 / Amino acid/peptide transporter / GAACGCTTGGCCTACTATGG / GACATTTGTTGCGGCTGAA / 93 / 1.970
87 / EE193726 / CDSP34-fibrillin / GCTGGAGGCTAAAAACCCAAC / CGACAACAAAGGAAACAATCC / 109 / 1.908
88 / EE197220 / Oleosin OLE-2 / CCTCCTCTTCCTCCTCAATGG / GCCCGAAAGAACCAGAAGC / 113 / 1.848
89 / EE199428 / Steroid 5-alpha reductase / TCCGGCAACTTTTATCACCA / AAATCGAAGAGCCCACCAGT / 92 / 1.954
90 / EE200251 / Lactate dehydrogenase / GCCTCTGCTGGAGCTGTTTT / TGACCTCATAACCCGTGCTG / 109 / 1.867
91 / EE199585 / Geranyl diphosphate synthase / CTTTTCGGGTCCTCCACATC / CGCCCAGTAAGAGTAAGGTTGC / 102 / 1.812
92 / EE197326 / 1-deoxy-D-xylulose 5-phosphate synthase / CAGCGGATGAGGCTGAACTA / CCTTTGTTATTGGGCGGAAG / 124 / 1.906
93 / EE196665 / Aquaporin 1 / AAGCAGTGGGTGCTCAGTTG / CAGTGGTTGCCTCTCTGTGAA / 139 / 1.895
94 / EE199602 / Glucan endo 1,3 beta D glucosidase / TGTTTGTTATTGGGCTGCTG / CTTTTGATGTTGTTCTGGTTGC / 114 / 2.000
95 / EE196074 / Steroleosin-B STO-1 / GAGCTTCTTCTGGGATTGGAG / TGGAGCCTGTTGTCTCTTCG / 94 / 2.000
96 / EE195943 / Galactinol synthase / GTCTGCCTAGCCGTGCCTAT / CACCTTCCTCAACCCTTTGG / 92 / 1.969
97 / DV702649 / b-1,4-mannase synthase / TGGAGCAAGAAGTAGGCTCGT / CTTTCCATCCACCAGCATCA / 102 / 2.000
98 / DV689388 / Cinnamoyl CoA reductase / CAGAGGCTGGGCTGGAGATA / GCCTGCTGGTAGCTTTCGAC / 71 / 1.858
99 / DV703482 / Chitinase 134 / GAGATGGTGGATGACCCTGA / TCCCAGACAACAAAGCTGGT / 108 / 1.913
100 / EE195441 / Fructose-bisphosphate aldolase / CCACAGTCGATGGCAAGAAG / TGTTTGAGCCAGCAAGAGGA / 102 / 1.604
101 / AB084125 / 3,7-dimethylxanthine N-methyltransferase / ttccaatctatgcaccttcaac / agagaagccagcatcataagg / 113 / 2.000
102 / AM087675 / Sucrose synthase 2 / GGATGTGATGTGATGTGATGAA / CGGGTCGGGTCCAGTAGATA / 106 / 1.936
103 / DV703215 / Cell Wall Invertase / TCCCTTTCCTGAGATTTTTCCT / TGTTCAGTTTTCCACTTCTTGG / 91 / 1.883
104 / EE191819 / SPS sucrose phosphate synthase / TGGATTGTGGTGCTTCTACAGG / GCCTTCTGCCTTTTCCTTCTC / 80 / 1.822
C1 / AB015599 / Spermidine synthase / GAATAAATGGCCGAGCAGTTG / CATCAGCCATTCCAACAGAAG / 106 / 1.912
C2 / EE199348 / Cyclophylin / CCAGTCCGAATCCTTAACCAG / GAGCTCCATGCAGATACGGC / 94 / 1.876
C3 / EE197714 / Actine / TGGTGTGATGGTTGGTATGG / CCATGCTCGATTGGGTA / 99 / 2.000
C4 / EE197153 / B-tubulin / CCTGAGCTGACCCAACAAAT / CTGAACATGGCTGATGCAGT / 95 / 1.915
C5 / AJ519839 / SUI1 translation factor / tcttttgtgtcgcttgcatc / acccgagttatcagcattgg / 115 / 1.856
C6 / EE199621 / PolyUbiquitin 10 / CAGACCAGCAGAGGCTGATT / AGAACCAAGTGAAGGGTGGA / 100 / 1.944
C7 / EH662403 / 40S Ribosomal protein / GCAACCCGTAGGATTCTGAG / TCAGCAGGGACAACAACATC / 116 / 1.912
C8 / EF622216 / Kanamycin resistance gene / GCCATTCTCACCGGATTCAGTCGTC / ATCGGTCTGCGATTCCGACTC / 119 / 2.000

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