Supplementary Data, Figures

Supplementary Data, Figures

Supplementary Figure 1: Concentration-response curves based on size using only 141 and 83 day rearing times Salmotrutta populations combined, with potential misclassifications removed from the small and medium categories; methods for identifying potential misclassifications are as described in Section 2.3.1. Size categories are based on the 33rd and 67th percentile of the combined data where the small category includes fish with a final mass between 0.148 and 0.438 g, the medium category includes fish with a final mass between 0.446 and 0.593 g, and the large category includes fish with a final mass between 0.6 and 1.432 g. Concentrations are the mean of measured exposure concentrations in the two chronological age zinc exposures.

Supplementary Figure 2: Histograms of the weight of four chronological age exposures

Supplementary Figure 3: Histogram of fish weights combined from the 141, 106 and 83 day rearing times populations.

Supplementary Data, Figure 1

Supplementary Data, Figure 2

Supplementary Data, Figure 3

Supplementary Data, Tables

Supplementary Table 1.Concentration-response LC50(µg/L) and slope model estimates for size categories using only 141 and 83 day rearing times Salmotrutta populations combined, with potential misclassifications removed from the small and medium categories. Lower and upper confidence levels (LCL and UCL) reported.

Table 2.Likelihood ratio pairwise comparison of LC50 and slope model estimates for only the 141 and 83 days rearing time Salmotrutta populations combined. Bold p-values indicate a significant difference at α = 0.05.

Supplementary Table 1

Group / LC50 / LCL / UCL / Slope / LCL / UCL
Combined 83 and 141 day rearing time fish
Large / 405 / 334 / 476 / -3.36 / -4.61 / -2.10
Medium / 585 / 479 / 691 / -3.22 / -4.40 / -2.04
Small / 965 / 741 / 1190 / -1.98 / -2.77 / -1.20

Supplementary Table 2

LC50 / Slope
Comparison Groups / Estimate / p-value / Estimate / p-value
Size from combined 83 and 141 day rearing time fish
Small/Medium / 1.649 / 0.009 / 0.616 / 0.023
Small/Large / 2.380 / 0.000 / 0.591 / 0.013
Medium/Large / 1.444 / 0.017 / 0.960 / 0.875