Supplemental Table S3: Predicted Topology of BfpB and Putative Transmembrane Beta Strand Location

Putative Beta Strand / Direction / ProximalBoundary1 / Distal Boundary1 / Start & End / Sequence (length) / Length of PP Turn / Length of EC Loop / Confidence Level
P1 / Out / H116 (PP) / Y184 (EC) / Y169 -> F176 / YQGALSTF (8) / N/A / 14 / Medium
P2 / In / Y184 (EC) / T202 (PP) / Y190 -> F197 / YESGRIAF (8) / 7 / N/A / Medium
P3 / Out / T202 (PP) / Y215 (EC) / F205 -> G213 / FSISILPGG (9) / N/A / 34 / High
P4 / In / Y215 (EC) / S268 (PP) / F248 -> W255 / FDSDVDFW (8) / 16 / N/A / High
P5 / Out / Y272 (PP) / Y296 (EC) / Y272 -> V283 / YSISTSTSSVIV (12) / N/A / 33 / Medium
P6 / In / T319 (EC), Y315 (EC) / S328 (PP) / Y315 -> D321 / YNVTTTD (7) / 26 / N/A / Medium
P7 / Out / S342 (PP) / Y360 (EC) / F348 -> F357 / FAATSGTPSF (10) / N/A / 4 / High
P8 / In / Y360 (EC) / S410 (PP) / L361 -> L376 / LNGNGDSSNQVLLNLL (16) / 5 / N/A / Medium
P9 / Out / Y360 (EC) / S410 (PP) / V382 -> T391 / VSVVTSASV T (10) / N/A / 5 / Medium
P10 / In / Y360 (EC) / S410 (PP) / P397 -> G403 / PVPLKVG (7) / 4 / N/A / Medium
P11 / Out / S410 (PP) / S422 (EC) / Y408 -> S417 / YVSEIGTVLS (10) / N/A / 7 / High
P12 / In / S422 (EC) / S457 (EC) / A425 -> F434 / ASTSTVTSGF (10) / 17 / N/A / High
P13 / Out / S422 (EC) / S457 (EC) / Y452 -> S457 / YGVTLS (6) / N/A / 8 / High
P14 / In / S457 (EC) / S490 (TM), Y503 (EC) / F466 -> L478 / FDQATVNGTVIQL (13) / 7 / N/A / Medium
P15 / Out / S457 (EC)
S490 (TM) / Y503 (EC) / F486 -> A501 / FVQSSMLRNGNTLVLA (16) / N/A / 9 / High
P16 / In / Y503 (EC) / C539 (TM),
C-term (EC) / S510 -> F520 / SVDQGVGTTSF (11) / 13 / N/A / Medium
Intra-Pore Segment (IPS) / Out / Y503 (EC) / C539 (TM),
C-term (EC) / I538 -> K548 / ICITPRIIDLK (11) / N/A / 4 / Medium

1Topologyof anchor residue indicated as PP for periplasmic, EC for Extracellular, or TM for transmembrane.