Supplemental Table A: Spearman Correlation Between Physical Activity (MET-Hr/Wk), Inactivity

Supplemental Table A: Spearman correlation between physical activity (MET-hr/wk), inactivity (hr/wk), and body mass index (BMI, kg/m2) measures among 565 premenopausal women from the Nurses’ Health Study II.

Total activity / Walking / Vigorous activity / Total inactivity / Standing / Sitting / BMI
Total activity / 1.00 / 0.53** / 0.74** / 0.06 / 0.06 / 0.00 / -0.14*
Walking / 1.00 / 0.23** / 0.07 / 0.01 / 0.08 / -0.04
Vigorous activity / 1.00 / 0.05 / 0.11 / -0.05 / -0.08
Total inactivity / 1.00 / 0.79** / 0.51** / -0.05
Standing / 1.00 / -0.06 / -0.11*
Sitting / 1.00 / 0.09
BMI / 1.00

*p<0.01, **p<0.001

Supplemental Table B. Adjusted geometric mean levels1 of sex and growth hormones by total physical activity in premenopausal women, additionally adjusting for body mass index at blood collection

Total physical activity (MET-hr/wk)
n / <3 / 3-<9 / 9-<18 / 18-<27 / 27-<42 / 42+ / p-trend2
Sample size range / 59-76 / 80-116 / 100-132 / 62-76 / 60-75 / 57-72
Estradiol, pg/mL
Follicular / 425 / 45 / 51 / 48 / 46 / 42 / 47 / 0.54
Luteal / 422 / 126 / 123 / 119 / 119 / 111 / 110 / 0.04
Free estradiol, pg/mL
Follicular / 403 / 0.57 / 0.67 / 0.59 / 0.56 / 0.54 / 0.62 / 0.79
Luteal / 414 / 1.64 / 1.56 / 1.55 / 1.47 / 1.44 / 1.42 / 0.06
Estrone, pg/mL
Follicular / 428 / 37 / 42 / 41 / 44 / 37 / 38 / 0.59
Luteal / 458 / 80 / 80 / 79 / 80 / 67 / 72 / 0.01
Estrone sulfate, pg/mL
Follicular / 418 / 612 / 752 / 714 / 700 / 574 / 697 / 0.73
Luteal / 417 / 1398 / 1448 / 1387 / 1357 / 1188 / 1478 / 0.49
Testosterone, ng/dL / 509 / 22 / 25 / 23 / 25 / 22 / 22 / 0.11
Free testosterone, ng/dL / 505 / 0.19 / 0.20 / 0.19 / 0.20 / 0.18 / 0.18 / 0.11
Androstenedione, ng/dL / 516 / 98 / 108 / 104 / 114 / 99 / 99 / 0.43
DHEA3, ng/dL / 431 / 590 / 659 / 641 / 731 / 593 / 609 / 0.37
DHEAS, ug/dL / 430 / 72 / 83 / 76 / 90 / 73 / 72 / 0.21
Progesterone, ng/dL / 461 / 1248 / 1219 / 1102 / 1179 / 1124 / 1105 / 0.14
SHBG, nmol/L / 529 / 61 / 61 / 62 / 66 / 61 / 60 / 0.81
Prolactin, ng/mL / 522 / 15 / 16 / 14 / 16 / 15 / 14 / 0.41
GH, ng/mL / 435 / 0.50 / 0.49 / 0.45 / 0.58 / 0.49 / 0.52 / 0.53
IGF-1, ng/mL / 541 / 230 / 229 / 240 / 232 / 235 / 229 / 0.86
IGFBP-3, ng/mL / 541 / 4608 / 4543 / 4558 / 4615 / 4436 / 4605 / 0.92

1Adjusted for assay batch, age at blood draw, fasting status (follicular and luteal phase), time of day of blood draw (follicular and luteal phase), month of blood draw, luteal difference, follicular day, timing, duration of oral contraceptive use, family history of breast cancer, menstrual regularity, parity, and ovulatory status.

2Trend across continuous total activity in MET-hr/wk, using the Wald test.

3Abbreviations: DHEA = dehydroepiandrosterone; DHEAS = dehydroepiandrosterone sulfate; SHBG = sex hormone binding globulin, IGF = insulin-like growth factor, BP = binding protein, and GH = growth hormone

Supplemental Table C. Adjusted geometric mean levels1 of sex and growth hormones by total physical activity in premenopausal women, excluding those with anovulatory cycles and irregular menstrual cycles

Total physical activity (MET-hr/wk)
n / <3 / 3-<9 / 9-<18 / 18-<27 / 27-<42 / 42+ / p-trend2
Sample size range / 40-55 / 49-74 / 66-92 / 43-52 / 42-54 / 40-54
Estradiol, pg/mL
Follicular / 286 / 44 / 49 / 52 / 51 / 48 / 45 / 0.70
Luteal / 283 / 130 / 119 / 127 / 131 / 120 / 123 / 0.59
Free estradiol, pg/mL
Follicular / 267 / 0.58 / 0.63 / 0.63 / 0.60 / 0.53 / 0.58 / 0.64
Luteal / 279 / 1.73 / 1.58 / 1.61 / 1.58 / 1.58 / 1.59 / 0.44
Estrone, pg/mL
Follicular / 286 / 38 / 43 / 41 / 44 / 38 / 37 / 0.41
Luteal / 306 / 81 / 81 / 77 / 80 / 72 / 79 / 0.45
Estrone sulfate, pg/mL
Follicular / 273 / 617 / 784 / 657 / 712 / 611 / 727 / 0.97
Luteal / 274 / 1317 / 1387 / 1348 / 1386 / 1334 / 1844 / 0.18
Testosterone, ng/dL / 350 / 22 / 24 / 24 / 24 / 23 / 21 / 0.28
Free testosterone, ng/dL / 347 / 0.19 / 0.20 / 0.19 / 0.17 / 0.18 / 0.17 / 0.04
Androstenedione, ng/dL / 354 / 94 / 104 / 105 / 106 / 101 / 99 / 0.89
DHEA3, ng/dL / 307 / 617 / 622 / 626 / 659 / 593 / 602 / 0.40
DHEAS, ug/dL / 307 / 75 / 78 / 75 / 84 / 75 / 75 / 0.49
Progesterone, ng/dL / 308 / 1507 / 1408 / 1446 / 1594 / 1381 / 1461 / 0.64
SHBG, nmol/L / 366 / 56 / 60 / 63 / 76 / 63 / 62 / 0.16
Prolactin, ng/mL / 362 / 15 / 16 / 14 / 16 / 16 / 14 / 0.55
GH, ng/mL / 308 / 0.47 / 0.59 / 0.50 / 0.62 / 0.52 / 0.50 / 0.86
IGF-1, ng/mL / 375 / 227 / 233 / 247 / 227 / 242 / 236 / 0.77
IGFBP-3, ng/mL / 375 / 4690 / 4597 / 4620 / 4675 / 4393 / 4636 / 0.96

2Trend across continuous total activity in MET-hr/wk, using the Wald test.