Supplemental Table 1.Differentially expressed miRNAs in U87-CD133+ GSCs versusCD133-GCs.

No / Probe ID / miRNA / Expression inCD133+ GSCs / Fold change (U87-CD133+ GSCs / CD133－GCs)
1 / 10934 / hsa-miR-129-5p / Up / 2.79225
2 / 28737 / hsa-miR-106a / Up / 1.502143
3 / 17904 / hsa-miR-185* / Up / 1.62162
4 / 11038 / hsa-miR-299-5p / Up / 4.103897
5 / 27533 / hsa-miR-320 / Up / 1.703469
6 / 11067 / hsa-miR-338-3p / Up / 2.422264
7 / 11125 / hsa-miR-493* / Up / 2.56475
8 / 11164 / hsa-miR-519e / Up / 1.713851
9 / 17546 / hsa-miR-585 / Up / 1.950514
10 / 10999 / hsa-miR-20a / Up / 1.602571
11 / 21498 / hsa-miR-654-3p / Up / 1.5207516
12 / 17558 / hsa-miR-663 / Up / 2.193348
13 / 17354 / hsa-miR-637 / Up / 1.556392
14 / 32891 / hsa-miR-769-5p / Up / 10.73527
15 / 28047 / hsa-miR-890 / Up / 1.78793
16 / 19580 / hsa-let-7i / Up / 1.73231
17 / 11134 / hsa-miR-502-5p / Up / 2.01504
18 / 17662 / hsa-miR-574-3p / Up / 1.512054
19 / 17507 / hsa-miR-662 / Up / 2.16537
20 / 17398 / hsa-miR-634 / Up / 2.258229
21 / 17445 / hsa-miR-610 / Up / 1.542108
22 / 17305 / hsa-miR-642 / Up / 1.876067
23 / 27554 / hsa-miR-638 / Up / 1.939761
24 / 10983 / hsa-miR-18a / Up / 1.937578
25 / 11057 / hsa-miR-324-5p / Up / 2.01741
26 / 10977 / hsa-miR-183* / Up / 1.590286
27 / 29421 / hsa-miR-453 / Up / 1.855675
28 / 10972 / hsa-miR-181b / Up / 1.593196
29 / 17934 / hsa-miR-361-3p / Up / 1.541676
30 / 11059 / hsa-miR-326 / Up / 1.811717
31 / 17918 / hsa-miR-222* / Up / 1.778724
32 / 29204 / hsa-miR-378 / Up / 1.750351
33 / 5740 / hsa-miR-21 / Up / 1.666988
34 / 14285 / hsa-miR-487b / Up / 5.37806
35 / 28884 / hsa-miR-876-3p / Up / 1.603383
36 / 11163 / hsa-miR-519d / Up / 2.61309
37 / 13134 / hsa-miR-519c-5p / Up / 1.619154
38 / 17810 / hsa-miR-29b-1* / Up / 1.500114
39 / 17822 / hsa-miR-490-5p / Up / 5.41762
40 / 29802 / hsa-miR-144 / Up / 1.9559
41 / 10964 / hsa-miR-155 / Up / 1.576555
42 / 17563 / hsa-miR-644 / Up / 1.519852
43 / 17852 / hsa-miR-99a* / Up / 2.424991
44 / 13180 / hsa-miR-483-3p / Up / 6.67659
45 / 11167 / hsa-miR-520c-3p/520f / Up / 1.829378
46 / 17611 / hsa-miR-611 / Up / 2.4926
47 / 17411 / hsa-miR-147b / Up / 1.8582
48 / 19594 / hsa-miR-296-5p / Up / 4.231131
49 / 17828 / miRPlus_17828 / Up / 3.6498
50 / 17956 / miRPlus_17956 / Up / 2.44062
51 / 17821 / miRPlus_17821 / Up / 2.16241
52 / 28431 / miRPlus_28431 / Up / 1.852013
53 / 30317 / miRPlus_30317 / Up / 1.903126
54 / 17858 / miRPlus_17858 / Up / 4.70376
55 / 11201 / miRPlus_11201 / Up / 1.506539
56 / 17892 / miRPlus_17892 / Up / 3.14517
57 / 17899 / miRPlus_17899 / Up / 1.654167
58 / 28232 / miRPlus_28232 / Up / 1.98266
59 / 17833 / miRPlus_17833 / Up / 2.09723
1 / 17953 / hsa-miR-183 / Down / 0.63361
2 / 11124 / hsa-miR-492 / Down / 0.63672
3 / 17928 / hsa-miR-181a-2* / Down / 0.65681

Supplemental Table 2. Differentially expressed miRNAs in primary-CD133+ GSCs versusCD133- GCs.

No / Probe ID / miRNA / Expression inCD133+ GSCs / Fold change (primary-CD133+ GSCs / CD133- GCs)
1 / 10934 / hsa-miR-129-5p / Up / 8.404425
2 / 28737 / hsa-miR-106a / Up / 2.137944
3 / 17904 / hsa-miR-185* / Up / 5.304778
4 / 11038 / hsa-miR-299-5p / Up / 1.790445
5 / 27533 / hsa-miR-320 / Up / 1.807933
6 / 11067 / hsa-miR-338-3p / Up / 2.582066
7 / 11125 / hsa-miR-493* / Up / 2.332452
8 / 11164 / hsa-miR-519e / Up / 1.904732
9 / 17546 / hsa-miR-585 / Up / 1.519786
10 / 10999 / hsa-miR-20a / Up / 2.063932
11 / 21498 / hsa-miR-654-3p / Up / 2.849748
12 / 17558 / hsa-miR-663 / Up / 1.784772
13 / 17354 / hsa-miR-637 / Up / 5.164693
14 / 32891 / hsa-miR-769-5p / Up / 1.916442
15 / 28047 / hsa-miR-890 / Up / 2.311539
16 / 11176 / hsa-miR-526b / Up / 1.659861
17 / 10919 / hsa-miR-103 / Up / 1.804752
18 / 30684 / hsa-miR-302b / Up / 1.919081
19 / 27550 / hsa-miR-572 / Up / 3.018652
20 / 17427 / hsa-miR-200c / Up / 1.868727
21 / 13175 / hsa-miR-27b / Up / 1.822228
22 / 11037 / hsa-miR-299-3p / Up / 1.812757
23 / 11065 / hsa-miR-335 / Up / 2.516137
24 / 17582 / hsa-miR-661 / Up / 1.652012
25 / 17808 / hsa-miR-132* / Up / 1.700923
26 / 17818 / hsa-miR-27a* / Up / 2.22334
27 / 6880 / hsa-miR-297 / Up / 1.582921
28 / 11052 / hsa-miR-31 / Up / 3.49995
29 / 10618 / hsa-miR-524-5p / Up / 1.959491
30 / 11136 / hsa-miR-504 / Up / 2.70819
31 / 11064 / hsa-miR-331-3p / Up / 1.70476
32 / 11091 / hsa-miR-377 / Up / 1.889875
33 / 29379 / hsa-miR-452 / Up / 1.916873
34 / 14272 / hsa-miR-542-3p / Up / 1.632614
35 / 11135 / hsa-miR-503 / Up / 1.671791
36 / 5560 / hsa-miR-185 / Up / 1.8865
37 / 11159 / hsa-miR-518e / Up / 1.715121
38 / 17393 / hsa-miR-603 / Up / 1.709892
39 / 13147 / hsa-miR-96 / Up / 1.936611
40 / 4890 / hsa-miR-19b / Up / 2.45012
41 / 27898 / hsa-miR-768-3p / Up / 3.55788
42 / 32883 / hsa-miR-801 / Up / 1.781446
43 / 28019 / hsa-miR-10a* / Up / 1.596139
44 / 14310 / hsa-miR-409-5p / Up / 2.116929
45 / 10962 / hsa-miR-154 / Up / 14.8779
46 / 11048 / hsa-miR-30a / Up / 2.10012
47 / 14287 / hsa-miR-494 / Up / 1.828898
48 / 14328 / hsa-miR-124 / Up / 1.581203
49 / 10988 / hsa-miR-194 / Up / 1.939512
50 / 19596 / hsa-miR-30d / Up / 1.643298
51 / 11078 / hsa-miR-365 / Up / 2.218021
52 / 17936 / hsa-miR-16-1* / Up / 2.895076
53 / 17905 / hsa-miR-625* / Up / 3.934303
54 / 4610 / hsa-miR-126 / Up / 2.511434
55 / 17817 / hsa-miR-875-3p / Up / 2.07769
56 / 17289 / hsa-miR-616* / Up / 2.244917
57 / 17949 / hsa-miR-188-3p / Up / 2.404593
58 / 28736 / hsa-miR-17 / Up / 1.826226
59 / 29872 / hsa-miR-340 / Up / 1.503122
60 / 28336 / hsa-miR-671-5p / Up / 1.829129
61 / 17500 / hsa-miR-551b / Up / 3.790235
62 / 17565 / hsa-miR-30b / Up / 1.782705
63 / 17309 / hsa-miR-623 / Up / 1.663522
64 / 32731 / hsa-miR-190b / Up / 1.55978
65 / 27839 / miRPlus_27839 / Up / 1.746717
66 / 17896 / miRPlus_17896 / Up / 2.74535
67 / 17859 / miRPlus_17859 / Up / 1.874319
68 / 28454 / miRPlus_28454 / Up / 2.05255
69 / 32902 / miRPlus_32902 / Up / 1.906398
70 / 17890 / miRPlus_17890 / Up / 1.742452
71 / 17933 / miRPlus_17933 / Up / 1.594231
72 / 29878 / miRPlus_29878 / Up / 3.434901
73 / 17835 / miRPlus_17835 / Up / 1.507384
74 / 17955 / miRPlus_17955 / Up / 1.565572
75 / 11239 / miRPlus_11239 / Up / 1.749401
1 / 17510 / hsa-miR-602 / Down / 0.605654
2 / 17885 / hsa-miR-886-5p / Down / 0.249748
3 / 17943 / hsa-miR-760 / Down / 0.61905
4 / 17498 / hsa-miR-601 / Down / 0.555315
5 / 17443 / hsa-miR-608 / Down / 0.344342
6 / 30532 / hsa-miR-142-5p / Down / 0.592467
7 / 17505 / hsa-miR-654-5p / Down / 0.532704
8 / 17522 / hsa-miR-658 / Down / 0.659295
9 / 27692 / hsa-miR-886-3p / Down / 0.318311
10 / 10952 / hsa-miR-146a / Down / 0.475933
11 / 13169 / hsa-let-7d* / Down / 0.594191
12 / 10986 / hsa-miR-193a-3p / Down / 0.477647
13 / 17919 / hsa-miR-96* / Down / 0.660939
14 / 31867 / hsa-miR-145* / Down / 0.628104
15 / 11030 / hsa-miR-26a / Down / 0.659902
16 / 10919 / hsa-miR-103 / Down / 0.554093
17 / 17848 / miRPlus_17848 / Down / 0.536628
18 / 28432 / miRPlus_28432 / Down / 0.432208
19 / 28993 / miRPlus_28993 / Down / 0.550125
20 / 27564 / miRPlus_27564 / Down / 0.436042

Supplemental Table 3. Differentially expressed miRNAs in CD133+ GSCsversusCD133- GCs.

No / Probe ID / miRNA / Expression inCD133+ GSCs / Fold change (U87-CD133+ GSCs / CD133- GCs) / Fold change (primary-CD133+ GSCs / CD133- GCs)
1 / 10934 / hsa-miR-129-5p / Up / 2.79225 / 8.404425
2 / 28737 / hsa-miR-106a / Up / 1.502143 / 2.137944
3 / 17904 / hsa-miR-185* / Up / 1.62162 / 5.304778
4 / 11038 / hsa-miR-299-5p / Up / 4.103897 / 1.790445
5 / 27533 / hsa-miR-320 / Up / 1.703469 / 1.807933
6 / 11067 / hsa-miR-338-3p / Up / 2.422264 / 2.582066
7 / 11125 / hsa-miR-493* / Up / 2.56475 / 2.332452
8 / 11164 / hsa-miR-519e / Up / 1.713851 / 1.904732
9 / 17546 / hsa-miR-585 / Up / 1.950514 / 1.519786
10 / 10999 / hsa-miR-20a / Up / 1.602571 / 2.063932
11 / 21498 / hsa-miR-654-3p / Up / 1.5207516 / 2.849748
12 / 17558 / hsa-miR-663 / Up / 2.193348 / 1.784772
13 / 17354 / hsa-miR-637 / Up / 1.556392 / 5.164693
14 / 32891 / hsa-miR-769-5p / Up / 10.73527 / 1.916442
15 / 28047 / hsa-miR-890 / Up / 1.78793 / 2.311539

Supplementary Table 4. Primers used for qRT-PCR.

Gene / Forward Primer (5' to 3') / Reverse Primer (5' to 3')
TIMP2 / GCGGTCAGTGAGAAGGAAGTGG / CTTGCACTCGCAGCCCATCTG
GAPDH / TCTCTGCTCCTCCTGTT / ACTCCGACCTTCACCTT
CD133 / CCACAGATGCTCCTAAGGCTT / CCAGCTTTATGGGAGTCTTGG
SOX2 / CATCACCCACAGCAAATGACA / GCTCCTACCGTACCACTAGAACTT
POU5F1 / TATTCAGCCAAACGACCATCT / TCAGCTTCCTCCACCCACTT
NANOG / CTCTCCAACATCCTGAACCTC / GGTTCCCAGTCGGGTTCAC
BMI1 / CTCCACCTCTTCTTGTTTGC / GATGACCCATTTACTGATGATTT

Supplementary Table 5. Sequences of primers used in ChIP analysis of miR-20a and miR-106a.

Gene / Forward Primer (5' to 3') / Reverse Primer (5' to 3')
miR-20a-1 / CTCGTAAATGTTATGAATCC / TCTCCAGTAGAAATAGCA
miR-20a-2 / GATGAATAACTCCTGCTAAGAA / GGATGTTGTGAGTAAGATTGT
miR-20a-3 / ATTGCTGTATTTCTCGTAA / GAACCTCATAAACAACCA
miR-106a-1 / CACTCACTACCACCTTGTC / GTTTCTATTTCTTCCCTCCTC
miR-106a-2 / AAGAGAGAAGGGAAAATG / CACAGGAATTAGGAAACG
miR-106a-3 / TGGGACACTGGGTATACG / ACTCTACCGGATCACAGC

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