Supplemental Table 1: Detailed peptide information for proteins identified by MALDI-TOF/TOF-MS. The observed monoisotopic mass for each unique matching peptide is indicated, with the difference between observed and calculated mass shown in Da. When fragmentation information was obtained for a peptide, the individual ion score and ion confidence interval are given. The putative sequence of each peptide together with its start and end positions in the sequence are also indicated. Fragment ion masses are represented withtwo decimals to better reflect MS/MS mass accuracy, but deviations from calculated masses were determined using internal 4 decimal place accuracy.
Band 7 - Hypothetical protein (Strongylocentrotus purpuratus) and Tubulin beta chain (Paracentrotus lividus)Obs. Mass / +/- Da / Position / Sequence / Ion Score / Ion C.I. (%)
1053.60
1130.59
1143.63
1229.59
1258.69
1271.72
1385.69
1601.82
1620.83
1958.98 / -0.036
0.0378
-0.0379
-0.042
-0.047
-0.0466
-0.0502
-0.0758
-0.0721
-0.075 / 310-318
242-251
253-262
381-390
242-252
252-262
381-391
63-77
263-276
104-121 / YLTVAAIFR
FPGQLNADLR
LAVNMVPFPR
ISEQFTAMFR
FPGQLNADLRK
KLAVNMVPFPR
ISEQFTAMFRR
AVLVDLEPGTMDSVR
LHFFMPGFAPLTSR
GHYTEGAELVDSVLDVVR / 36
65
41 / 32.7
99.7
31.9
Similar to tubulin alpha 2 isoform 2 (Strongylocentrotus purpuratus) and tubulin alpha chain (Paracentrotus lividus)
Obs. Mass / +/- Da / Position / Sequence / Ion Score / Ion C.I. (%)
1457.87
1687.89
1718.88
1854.90
2415.10 / -0.0699
-0.0698
-0.0712
-0.0559
-0.1018 / 229-242
64-64
215-228
373-389
84-104 / LIGQIVSSITASLR
AVFVDLEPTVVDEVR
NLDIERPTYTNLNR
AVCMLSNTTAIAEAWAR
QLFHPEQLITGKEDAANNYAR / 99
32 / 100
0
Band 8 - Muscle actin (Strongylocentrotus purpuratus) and actin, cytoskeletal (Heliocidariserythrogramma)
Obs. Mass / +/- Da / Start Seq. / Sequence / Ion Score / Ion C.I. (%)
923.57
976.45
1132.53
1187.57
1198.70
1354.62
1415.75
1516.70
1790.89
1954.06
2201.05 / -0.0351
-0.0189
-0.0257
-0.0204
-0.0510
-0.0476
-0.0618
-0.0522
-0.0738
-0.0832
-0.0547 / 330-337
20-29
198-207
41-51
30-40
52-63
86-96
361-373
240-255
97-114
293-313 / IIAPPERK
AGFAGDDAPR
GYSFTTTAER
HQGVMVGMGQK
AVFPSIVGRPR
DSYVGDEAQSKR
IWHHTFYNELR
QEYDESGPSIVHR
SYELPDGQVITIGNER
VAPEEHPVLLTEAPLNPK
DLYANTVLSGGSTMFPGIADR / 54
26
36
79
131 / 98.1
0
0
99.9
100
Supplemental Table 1 (cont.)
Gel band 9 - Histone H2B-1, sperm specific (Strongylocentrotus purpuratus)Obs. Mass / +/- Da / Position / Sequence / Ion Score / Ion C.I. (%)
953.60
1135.57
1196.60
1291.67
1777.79 / -0.0292
-0.0274
-0,0277
-0,0271
0,0288 / 115-123
50-58
62-72
49-58
73-87 / LLLPGELAK
ESYGIYIYK
QVHPDTGISSR
RESYGIYIYK
GMSIMNSFVNDVFER / 53
50 / 99.9
99.9
Histone H2B-2, sperm (Parechinus angulosus)
Obs. Mass / +/- Da / Position / Sequence / Ion Score / Ion C.I. (%)
953.60
1135.57
1196.60
1291.67
1777.79 / -0.0301
-0.0343
-0.0311
-0.0343
-0.0334 / 119-127
54-62
66-76
53-62
77-91 / LLLPGELAK
ESYGIYIYK
QVHPDTGISSR
RESYGIYIYK
AMSVMNSFVNDVFER / 59 / 99.5
Gel band 11 - Histone H3 (Strongylocentrotus purpuratus)
Obs. Mass / +/- Da / Position / Sequence / Ion Score / Ion C.I. (%)
831.49
844.50
850.43
1032.59
1349.71 / -0.0299
-0.0284
-0.0278
-0.0249
-0.0291 / 58-64
117-123
68-74
36-44
68-78 / STELLIR
RVTIMPK
EIAQDFK
YRPGTVALR
EIAQDFKTELR / 31
37
95 / 0
0
100
Gel band 12 - Histone H2A-3 (Strongylocentrotus purpuratus)
Obs. Mass / +/- Da / Position / Sequence / Ion Score / Ion C.I. (%)
850.53
944.53
1692.90 / -0.0281
0.0053
-0.0494 / 82-88
21-29
82-95 / HLQLAIR
AGLQFPVGR
HLQLAIRNDEELNK / 54
77 / 99.9
100
Histone H2A, sperm (Lytechinus pictus)
Obs. Mass / +/- Da / Position / Sequence / Ion Score / Ion C.I. (%)
850.53
944.53
1692.90 / -0.0281
0.0053
-0.0494 / 69-75
8-16
69-82 / HLQLAIR
AGLQFPVGR
HLQLAIRNDEELNK / 54
77 / 95.1
99.9
Gel band 13 – Histone H4 (Paracentrotus lividus) and similar to histone H4 (Strongylocentrotus purpuratus)
Obs. Mass / +/- Da / Position / Sequence / Ion Score / Ion C.I. (%)
989.58
1180.62
1325.75
1336.72
1349.63
1466.80
1481.85
1577.88
2164.08 / -0.0233
-0.0336
-0.0349
-0.0337
-0.0483
-0.036
-0.037
-0.0324
-0.0371 / 60-67
46-55
24-35
45-55
68-78
80-92
24-36
46-59
60-77 / VFLENVIR
ISGLIYEETR
DNIQGITKPAIR
RISGLIYEETR
DAVTYCEHAKR
TVTAMDVVYALKR
DNIQGITKPAIRR
ISGLIYEETRGVLK
VFLENVIRDAVTYCEHAK / 72
86
92
73 / 99.9
99.9
100
99.9