Supplemental Table 1: a Full List of Differentially Expressed Genes in Early-Onset Rectal

Supplemental Table 1:A full list of differentially expressed genes in early-onset rectal tumors.A list of differentially expressed genes between tumor and matching non-involved rectal tissues in patients 50 years old or younger.A gene is estimated to have 2^(log fold change) times its expression in baseline samples (non-involved rectal tissue).The 95% confidence interval for the log fold change is also presented, along with p-values and false discovery rates (FDR).

Supplemental Table 2:A full list of differentially expressed genes in late-onset rectal tumors. A list of all genes differentially expressed between tumor and matching non-involved rectal tissues in patients 65 years old or older.A gene is estimated to have 2^(log fold change) times its expression in baseline samples (non-involved rectal tissue).The 95% confidence interval for the log fold change is also presented, along with p-values and false discovery rates.

Supplemental Figure 1:Representative Path view graph for regulation of MAPK signaling in early-onset rectal cancer.Each node represents a protein; node colors are derived from the differential expression of all genes corresponding to the protein. White indicates a pathway gene not present in the data. Red indicates a high log fold-change (up-regulation); green indicates a low log fold-change (down-regulation).

Supplemental Figure 2:Representative Path view graph for regulation of PI3K-AKT signaling in late-onset rectal cancer.Each node represents a protein; node colors are derived from the differential expression of all genes corresponding to the protein. White indicates a pathway gene not present in the data. Red indicates a high log fold-change (up-regulation); green indicates a low log fold-change (down-regulation).

Supplemental Figure 3:Age-dependent statistically significant gene expression changes in rectal tumors.Heat map showing21 significantly different expression changes in rectal tumors between the two age groups out of 770 genes assayed.Significance is defined by difference of mean gene expression changes >2 fold and a p-value <0.05.Multi-experiment viewer 4.9 was used to cluster both samples and genes based on similarity in expression patterns.