Supplemental material

Legend of figures

Fig. 1 Chitinase Lbchi31 gene cDNA and DNA sequences. Signal peptide is at N-terminal of LbCHI31. Intron sequence is shown in shade section.

Fig.2 Multiple sequence alignment of chitin-binding domain from 32 fungal species/strains. ACN55075: Chimonanthus praecox; ACL81177: Chimonanthus praecox; CAB01591: Persea americana; AAF04454: Poa pratensis; AAF04453: P. pratensis; AAR11388: Triticum aestivum; CAA53626: T. aestivum; BAG12896: ACJ23248: Festuca arundinacea; BAF47270: AAT40032: Zea diploperennis; AAT40017: Z. mays subsp. Parviglumis; ACJ62111: AAT40029: Z. diploperennis; AAY82488: CAA71402: Medicago truncatula; ACM45713: Pyrus pyrifolia; AAG23965: Vigna sesquipedalis; CAA61278: Vigna unguiculata; AAR27240: Phaseolus vulgaris; AAM77665: AAT40738: Nepenthes khasiana; AAP03087: Galega orientalis; AAP03089: G. orientalis; P36907: Pisum sativum; AAF17593: Glycine max; AAP03088: G. orientalis; AAB01895: Castanea sativa; ABD92819: Limonium bicolor; ABD66068: Momordica charantia; CAN62784: Vitis vinifera; CAC14015: V. vinifera.

Fig. 1

1 ca aca 5

6 atg aaa acg aca ctc atc cta acc gcc gcc gct ctc ccc ttt ctt ctc ttg att agc ttc 65

M K T T L I L T A A A L P F L L L I S F

66 tca tcc gcc gag cag tgc ggt tct caa gcc ggt gga gct gtt tgt ccc aac gga cta tgc 125

S S A E Q C G S Q A G G A V C P N G L C

126 tgc agc aag tac gga tgg tgc ggc tca aca gac aca tac tgt aaa gac ggt tgc cag agt 185

C S K Y G W C G S T D T Y C K D G C Q S

186 caa tgc ggt ggc act act cct act cct act act cct acg cct acg cct act cag gga ggt 245

Q C G G T T P T P T T P T P T P T Q G G

246 gat gtc agc tcc atc atc agt cag agc aat ttc gac gaa atg ctt aaa cac cgc aac gat 305

D V S S I I S Q S N F D E M L K H R N D

306 aac gcc tgc tct ggc cat gga ttc tat acc tac gct gct ttc atc agt gct gct cag tct 365

N A C S G H G F Y T Y A A F I S A A Q S

366 ttc cct gga ttc ggg aca act ggt gac aca gac acc cgt aaa cgt gaa ctc gct gcc ttc 425

F P G F G T T G D T D T R K R E L A A F

426 ctt gga caa acc tca cac gag acc act gga gga tgg gcg gct gca cca gac ggc cca tac 485

L G Q T S H E T T G G W A A A P D G P Y

486 gct tgg gga tac tgc ttc gtt caa gaa caa ggt gca tca ggt gcc ttc tgt act tca cct 545

A W G Y C F V Q E Q G A S G A F C T S P

546 gaa tat cca tgc gca cct gga aaa cag tac tat ggt cgt ggt cca atc cag cta aca cac 605

E Y P C A P G K Q Y Y G R G P I Q L T H

606 aacgtaagtaatcatcaatttctctcttttacccggcctgtcttcgtaatttaatttaatcaatacttccttcag tac 683

N Y

684 aac tac gct caa gct ggg aca gca att aat caa gac tta atc aac aac ccg gat ctg gta 743

N Y A Q A I N Q D L I N N P D L V A T D

744 gca aca gac ccg act ata tca ttc aag aca gca ata tgg ttc tgg atg aca ccg cag agc 803

G T A P T I S F K T A I W F W M T P Q S

804 aac aag cca tcc agc cac gat gtc atc acc ggg aag tgg acg ccg tcg tca gct gac agt 863

N K P S S H D V I T G K W T P S S A D S

864 gcg gcc gga aga gtt cct ggg tac gga gtc atc act aac ata att aac ggc ggt ctt gaa 923

A A G R V P G Y G V I T N I I N G G L E

924 tgt ggg aag gga tcc att gct aag caa gag gat agg att ggg ttc tat aag agg tat tgt 983

C G K G S I A K Q E D R I G F Y K R Y C

984 gac gtg ctg gga gtt ggg tac ggg aac aac ttg gat tgc aat agc cag agg cct ttt gct1043

D V L G V G Y G N N L D C N S Q R P F A

1044 tga tga aat tag tca aca tca aaa tgg ttt att tat taa tgg cac tga tat agt cat acc1103

*

1104 aag att atg att gta ata aaa tgt ata cac gat act ata aag aat ttc cta ttc gtt aaa1163

1164 aaa aaa aaa aaa aaa 1178

Fig.2

ACN55075 EDCGSQAGGALCPGGLCCSKFGFCGTTADYCGTGCQSQC 59

ACL81177 EDCGSQAGGALCPGGLCCSKFGFCDTTADYCGTGCQSQC 59

CAB01591 EQCGRQAGGALCPGGLCCSQFGWCGSTSDYCGPTCQSQC 64

AAF04454 EQCGSQAGGATCPNCLCCSKFGFCGTTSDYCGTGCQSQC 59

AAF04453 EQCGSQAGGATCPNCLCCSKFGFCGNTSDYCGTGCQSQC 59

AAR11388 EQCGSQAGGATCPNCLCCSKFGFCGTTSDYCGTGCQSQC 59

CAA53626 EQCGSQAGGATCPNCLCCSKFGFCGSTSDYCGNGCQSQC 59

BAG12896 EQCGSQAGGATCPNCLCCSRFGFCGSGSDYCGAGCQSQC 59

ACJ23248 QQCGSQAGGATCANCLCCSQYGYCGSTSAYCGAGCQSQC 60

AAT40029 EQCGSQAGGALCPNCLCCSQYGWCGSTSDYCGSGCQSQC 61

AAT40032 EQCGSQAGGALCPNCLCCSQFGWCGSTSDYCGSGCQSQC 61

AAT40017 EQCGSQAGGALCPNCLCCSQFGWCGSTSDYCGSGCQSQC 61

ACJ62111 EQCGSQAGGALCPNCLCCSQFGWCGSTSDYCGSGCQSQC 62

BAF47270 QQCGSQAGGALCPGGLCCSQWGYCGDTLPYCGDGCQSQC 61

AAY82488 DQCGSQAGGAVCPGGLCCSKFGWCGNTNEYCGDGCQSQC 60

CAA71402 DECGKQAGGALCPGGLCCSKFGWCGSTGDYCGDGCQSQC 62

AAP03088 EQCGRQAGGALCPGGLCCSKFGWCGSTGDYCGDGCQSQC 65

AAG23965 -QCGSQAGGALCPGGLCCSQFGWCGSTDDYCGKGCQSQC 38

CAA61278 EQCGSQAGGALCPGGLCCSQFGWCGSTDDYCGKGCQSQC 63

AAR27240 EQCGRQAGGALCPGGNCCSQFGWCGSTTDYCGKDCQSQC 65

AAM77665 EQCGSQAGGALCPGGLCCSQFGWCGSNNDYCGPGCQSQC 62

AAT40738 EQCGSQAGGAACPGGLCCSQFGWCGTTDDYCEAGCQSQC 62

AAP03087 EQCGSQANGAVCPNGLCCSQFGYCGNTDQYCGAGCQSQC 63

AAP03089 EQCGSQANGAVCPNGLCCSKFGWCGNTDPWCGDGCQSQC 63

P36907 EQCGSQAGGAVCPNGLCCSKFGFCGSTDPYCGDGCQSQC 62

AAF17593 EQCGTQAGGALCPNRLCCSKFGWCGDTDSYCGEGCQSQC 62

ACM45713 EQCGRQAGGAVCPNGLCCSQFGWCGTTSDYCTTGCQSQC 58

AAB01895 EQCGRQAGGAACANNLCCSQFGWCGNTAEYCGAGCQSQC 57

ABD66068 EQCGRQANGALCPNRLCCSQHGWCGSTDEYCKNGCQSQC 59

ABD92819 EQCGSQAGGAVCPNGLCCSKYGWCGSTDTYCKDGCQSQC 62

CAC14015 EQCGRQAGGALCSGGLCCSQYGYCGSTSAYCSTGCQSQC 61

CAN62784 EQCGRQAGGALCSGGLCCSQYGYCGSTSAYCSTGCQSQC 61

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