Deng Pan et al. MALT1 is required for EGFR induced NF-kB activation and contributes to EGFR-driven lung cancer progression
Supplemental Figures
Supplementary Figure 1. A431 cells with a MALT1 knockdown (left) and MALT1-deficient MEFs (right) were reconstituted with wild-type MALT1 (shMALT1WT or Malt1-/-WT) or a protease-deficient C464A mutant (shMALT1C464A or Malt1-/-C464A). Lysates of these cells were analyzed by immunoblotting with anti-MALT1 or anti-Flag antibodies.
Supplementary Figure 21. (a) Jurkat cells were stimulated with PMA and ionomycin (20ng/ml; 100ng/ml) for the indicated periods with or without 75 µM MALT1 inhibitor z-VRPR-Fmk. Whole cell lysates were analyzed by immunoblotting with the indicated antibodies. (b) A431 cells were stimulated with EGF (100ng/ml) or TNFα (10ng/ml) for 60 minutes, with or without 75 µM MALT1 inhibitor z-VRPR-Fmk. Nuclear lysates were isolated and subjected to gel shift analysis for NF-κB activation.
Supplementary Figure 3. The lysates of A431 cells with control knockdown (shCtl), MALT1 knockdown (shMALT1) and TRAF6 knockdown (shTRAF6) were analyzed by immunoblotting using indicated antibodies.
Supplementary Figure 4. Transwell migration assays were performed using shCtl A431 cells, shTRAF6 A431 cells and shCtl A431 cells with IKK inhibitor TPCA (1μM) treatment, respectively. **** p<0.0001.
Supplementary Figure 5. Transwell migration assays were performed using shCtl (A431 and HCC827) cells, shMALT1 cells and shCtl cells treated with MALT1 inhibitor z-VRPR-Fmk (75μM), respectively. **** p<0.0001; ns, no significance.
Supplementary Figure 6. HCC827 cells with MALT1 knockdown (shMALT1) and control cells (shCtl) were subjected to the MTT assay to determine their proliferation rates at 24, 48, 72 hours. OD, optical density.
Supplementary Figure 74. Related to Figure 3d. Representative pictures showing the size of shCtl and shMalt1 colonies.
Supplementary Figure 2. Transwell migration assays were performed using shCtl A431 cells, shTRAF6 A431 cells and shCtl A431 cells with IKK inhibitor TPCA (1μM) treatment, respectively.
Supplementary Figure 3. HCC827 cells with MALT1 knockdown (shMALT1) and control cells (shCtl) were subjected to the MTT assay to determine their proliferation rates at 24, 48, 72 hours. OD, optical density.
Supplementary Figure 8. Control (shCtl) and MALT1-silenced (shMalt1) HCC827 cells were treated with IL-6 neutralizing antibodies and IKK inhibitor TPCA (1μM) for 12 hours, respectively. Lysates of these cells were analyzed by immunoblotting with indicated antibodies. Band intensity was quantified by using Image J.
Supplementary Figure 4. Related to Figure 3d. Representative pictures showing the size of shCtl and shMalt1 colonies.
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