Supplemental Figure 1. Changes to Seed FA in Auxin Signaling Mutants Axr2-1
Supplemental Figure 1.Changes to Seed FA in Auxin Signaling Mutants axr2-1.
The result ispresented as mean values±SD from three biological replicates.
(A) Percentage changes of the FA components in the axr2-1 mutant seed compared withcol-0.
(B) Total FA content(mgg–1 seed) of the seed FAs in the axr2-1 mutant seed compared with col-0.
Supplemental Table 1.Repressed Genes Encoding Proteins Involved in Gene Replication, Transcription, and Protein Translations during 17–21DAF of B. napus Seed Development.
B194G011 / 30S ribosomal protein S16B198F051 / 40S ribosomal protein S19
B285B121 / 40S ribosomal protein S26
B225A091 / 40S ribosomal protein S8 (RPS8A)
B248D121 / Putative 40S ribosomal protein S16
B280G011 / Putative 40S ribosomal protein S19
B081F071 / Putative 40S ribosomal protein S3A
B424B051 / Putative 40S ribosomal protein SA
B396E051 / Putative 50S ribosomal protein L33
B282F011 / 60S acidic ribosomal protein P1
B284G091 / 60S acidic ribosomal protein P2
B161A081 / 60S ribosomal protein L10
B099C021 / 60S ribosomal protein L10A
B239B041 / 60S ribosomal protein L12
B242G091 / 60S ribosomal protein L13a
B287G061 / 60S ribosomal protein L13A-like
B423C011 / 60S ribosomal protein L18
B281E101 / 60S ribosomal protein L18A
B154E101 / 60S ribosomal protein L27A
B212E061 / 60S ribosomal protein L27A
B246A051 / 60S ribosomal protein L29
B436E021 / 60S ribosomal protein L31
B188C071 / 60S ribosomal protein L34
B178B011 / 60S ribosomal protein L35
B170E101 / 60S ribosomal protein L37
B202C121 / 60S ribosomal protein L5
B003E061 / 60S ribosomal protein L7A
B211E081 / 60S ribosomal protein L7A protein
B279E031 / 60S ribosomal protein L7A protein
B206H101 / 60S ribosomal protein-like
B057G011 / 60S ribosomal protein-like
B289H111 / Putative 60S ribosomal protein
B399D011 / Putative 60S ribosomal protein L1
B226C011 / Putative 60S ribosomal protein L6
B239C051 / Putative ribosomal protein
B034H021 / Putative ribosomal protein s19 or s24
B154E051 / Putative ribosomal protein s19 or s24
B166D011 / Ribosomal protein L14-like protein
B078H091 / Ribosomal protein L15.DL4730C
B209B041 / Ribosomal protein L16
B176G071 / Ribosomal protein S27
B269G081 / Ribosomal protein S30 homolog
B147H021 / Ribosomal protein S30 homolog
B224H081 / Ribosomal protein S4/S9 related
B436C111 / Ribosomal protein S5 family
B436D021 / Plastid ribosomal protein L34 precursor-related
B245D081 / Contains similarity to plastid-specific ribosomal protein 6
B069B111 / Chloroplast nucleotide DNA binding protein
B050H011 / Contains similarity to DNA-binding protein
B067H021 / DNA-binding protein family
B169G101 / DNA-binding protein RAV2, putative
B257A101 / DNA-binding protein RAV2, putative
B121C031 / DNA-directed RNA polymerase subunit-related
B272A011 / DNA-directed RNA polymerase, subunit B
B292G031 / Histone H1
B367F101 / Histone H2A
B263A061 / Histone H2A, putative
B264D111 / Histone H2A, putative
B140B071 / Histone H2A, putative
B144F091 / Histone H2A-like protein
B285B061 / Histone H2A-like protein
B296C101 / Histone H2A-like protein
B055A061 / Histone H3.3
B245F031 / Histone H3.3
B101D031 / Histone H3-like protein
B243A101 / Histone H3-like protein
B424C051 / Histone H3-like protein
B242C091 / Histone H3-like protein
B302D061 / Histone H4-like protein
B370E121 / Histone H4-like protein
B127C071 / Nuclear RNA binding protein A-like protein
B194H081 / Nuclear RNA binding protein A-like protein
B212A051 / Nuclear RNA binding protein, putative
B004B081 / Nucleotide DNA-binding-like protein
B374C091 / Putative DNA binding protein
B222G091 / Putative DNA polymerase delta small subunit
B049H041 / Putative DNA replication licensing factor, mcm5
B198D081 / Putative DNA-binding protein
B364C111 / Putative eukaryotic translation initiation factor 5
B122E051 / RNA binding protein-like
B188F121 / RNA helicase, putative
B067C111 / RNA helicase, putative
B063H101 / RNA recognition motif (RRM)-containing protein
B239B051 / RNA-binding protein, putative
B172F091 / RNA-binding protein-like
B052C031 / 67kD chloroplastic RNA-binding protein, P67
B064G021 / Transcription regulator-like
B253F071 / Transcriptional factor B3 family
B237H111 / Translation initiation factor
B375H021 / Translational inhibitor protein, putative
B223A021 / Homeodomain transcription factor (ATHB-6)
B233A031 / MYB family transcription factor
B090F061 / MYB-related transcription activator-like
B428E061 / Contains similarity to MYB-related transcription factor
B242B081 / DEAD-box protein
B191E011 / bHLH protein
B396F011 / SUVH9
B296G121 / Glu-tRNA synthetase
B121C101 / Aspartate–tRNA ligase-like protein
B370F121 / RNase H domain-containing protein
B001E061 / Putative histone deacetylase
Supplemental Table 2. GC–MS Quantification of Seed Total FA from Wild-Type, axr1-3, tir1-1, and coi1.
FA16:0 / FA16:1 / FA18:0 / FA18:1 / FA18:2 / FA18:3 / FA20:0 / FA20:1 / FA20:2 / FA22:0 / FA22:1WT / 11.94±0.31 / 0.64±0.02 / 2.81±0.06 / 14.85±0.33 / 29.72±0.35 / 20.67±0.54 / 1.83±0.15 / 14.89±0.26 / 1.55±0.06 / 0.14±0.02 / 0.96±0.06
axr1-3 / 10.82±0.34 / 0.61±0.01 / 3.06±0.11 / 16.92±0.46 / 27.07±0.27 / 20.29±0.62 / 2.00±0.03 / 16.49±0.37 / 1.43±0.09 / 0.16±0.01 / 1.15±0.04
tir1-1 / 12.14±0.16 / 0.68±0.03 / 2.77±0.15 / 14.75±0.44 / 30.17±0.46 / 20.30±0.57 / 1.91±0.05 / 14.58±0.32 / 1.58±0.06 / 0.17±0.01 / 0.95±0.07
coi1 / 10.00±0.08 / 0.43±0.01 / 2.84±0.11 / 18.79±0.27 / 26.72±0.43 / 20.50±0.56 / 1.80±0.07 / 16.42±0.46 / 1.36±0.05 / 0.12±0.01 / 1.02±0.06
The contents of FA compositions are presented as mean values±SD from four biological replicates. Significant differences are marked with overstriking (P0.01). Values show the relative amounts (%).
Supplemental Table 3. Total Content of Seed FA in Wild-Type, axr1-3, tir1-1, and coi1.
WT / axr1-3 / tir1-1 / coi1335.13±16.64 / 392.05±20.59 / 311.76±15.75 / 463.99±15.57
The contentsare presented as mean values±SD from four biological replicates. Significant differences are marked with overstriking (P0.01). Valuesshow the total FA content (mgg–1 seed).
Supplemental Table 4.Genes Showing Similar Expression Patternsto FA Synthesis-Related Genes.
EL628140 / Adenine nucleotide translocaseEL624240 / Adenylate kinase
EL627695 / Alcohol dehydrogenase
EL625240 / Aspartate aminotransferase (Asp3)
EL627014 / Aspartate aminotransferase, cytoplasmic isozyme 2
EL628699 / At1g04990/F13M7_1
EL624406 / At1g32900/F9L11_8
EL626992 / AT3g25860/MPE11_1
EL624305 / AT4g35220/F23E12_220
EL625791 / AT5g25980/T1N24_18
EL626275 / caffeoyl-CoA O-methyltransferase-like protein
EL627837 / Chalcone synthase
EL629419 / Chalcone synthase
EL627388 / Chalcone synthase
EL630625 / Chalcone synthase
EL628716 / Cinnamoyl-CoA reductase-like protein
EL626742 / Citrate synthase-like protein
EL629366 / Coatomer zeta subunit –related
EL626408 / Contains similarity to an unknown protein F7A7_100
EL626578 / Contains similarity to disease resistance protein
EL628361 / Copper chaperone (CCH)-related
EL680959 / Cysteine proteinase inhibitor B (cystatin B)-related
EL628293 / Dihydroxyacetone 3-phosphate reductase
EL630697 / DnaJ protein family
EL629124 / DnaJ protein, putative
EL625336 / Epithiospecifie
EL626376 / ESTs gb|AI993254, gb|T76141 and gb|AA404864 come from this gene
EL625828 / Expressed protein
EL624357 / Expressed protein
EL624113 / Expressed protein
EL630032 / Expressed protein
EL630054 / Expressed protein
EL623452 / F21J9.12
EL623956 / Gamma-glutamyltransferase-like protein
EL626586 / Gamma-VPE (vacuolar processing enzyme)
EL623487 / Similar to unknown protein
EL626260 / Glutathione S-transferase
EL626901 / Glycine-rich protein
EL628772 / GTPase activator protein-related
EL628246 / Hypothetical protein
EL629158 / Hypothetical protein
EL680945 / Hypothetical protein
EL629950 / Hypothetical protein
EL630217 / Hypothetical protein At2g17710
EL628297 / Hypothetical protein At2g17730
EL627495 / Hypothetical protein At2g22340
EL625177 / Hypothetical protein At2g25650
EL628982 / Hypothetical protein F15J5.120
EL628229 / Hypothetical protein F16J13.30
EL628073 / Inorganic pyrophosphatase-like protein
EL624965 / L-allo-threonine aldolase homolog F22O13.11
EL630490 / Leucine-rich repeat extensin family
EL629775 / Lipoamide dehydrogenase
EL627205 / Metal transporter, putative (ZIP4)
EL629896 / MtN19-like protein
EL627845 / MutT/nudix family protein
EL628725 / NADPH-ferrihemoprotein reductase (EC 1.6.2.4) ATR2
EL626282 / Nitrilase 2
EL630690 / Nodulin MtN3 family protein
EL629086 / Outer membrane lipoprotein-like
EL630778 / Peroxidase, putative
EL627437 / Phosphoglucomutase
EL627666 / Poly(A)-binding protein
EL628635 / P-protein-like protein
EL625850 / Probable tropinone reductase
EL624152 / Protein kinase-related
EL629184 / Putative acetone-cyanohydrin lyase
EL630177 / Putative AMP deaminase
EL625930 / Putative chorismate mutase/prephenate dehydratase
EL627968 / Putative cytochrome P450
EL625014 / Putative dehydrogenase
EL630608 / Putative GDSL-motif lipase/hydrolase
EL624980 / Putative leucoanthocyanidin dioxygenase (LDOX)
EL628669 / Putative phytosulfokine peptide precursor
EL628026 / Putative poly-A binding protein
EL625241 / Putative protein
EL625357 / Putative protein
EL625376 / Putative protein
EL626255 / Putative protein
EL626630 / Putative serine carboxypeptidase II
EL626790 / Putative splicing factor
EL629512 / RNA-binding protein MEI2, putative
EL626669 / S-adenosylmethionine synthetase
EL629851 / senescence-specific cysteine protease
EL628126 / Similar to Cytochrome P450
EL628286 / Similar to Cytochrome P450
EL629853 / Similar to Cytochrome P450
EL628387 / Similar to pyrophosphate-dependent phosphofuctokinase beta subunit
EL624814 / Stress enhanced protein 2; SEP2
EL627715 / TOM3
EL629223 / UDP-glucose:protein transglucosylase
EL680953 / UDP-glucose:protein transglucosylase
EL628610 / Unknown protein
EL623014 / Unknown protein
EL624295 / Unknown protein
EL626186 / Unknown protein
EL627266 / Unknown protein
EL628139 / Unknown protein
EL628901 / Unknown protein
EL629151 / Unknown protein
EL623524 / VAMP (vesicle-associated membrane protein)-associated protein,putative
EL626852 / WRKY DNA-binding protein 21