Supplemental Data Set.
Jung et al.
Supplemental Data Set 1. Sequencing Analysis of ChIP-qPCR Products.
ChIP-PCR products were subcloned into the pGEM-7Zf(+) vector (Promega, Madison, WI) and sequenced usingthe Applied Biosystems 3730xl DNA Analyzer (Foster City, CA) at the National Instrumentation Center for Environmental Management (NICEM, Seoul National University, Seoul).
HOS1-FLC-P1_M13F.ab1
TAACACCACTTTATACCTTCGGCGATTGGGCCGAGTCGCATGCTCCTCTAGACTCGAGGATTCGGTACCCCGCATCAAAACTTCTTGGCACAGCTCCGAGTGTTACTGAAATGTTTGTGTGGCTCCAATAGAAAAGTTAATACCAATCATGAATCATCAATATCGTTACAAAATCAGTAATCAGTTTCACCCACTCCGAGGGGTTCGAAATCGATAAGCTTGGATCCGGAGAGCTCCCAACGCGTTGGATGCATAGCTTGAGTATTCTATAGTGTCACCTAAATAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGGTAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCCGTGCTACAGAGTTCTTGAGTGGTGGCCTACTACGGCTACACTAGAGACAGTATTGGTATCTGCGCCTTTTGCTGAG
CAGTAACATCGGAAAAAAGGAGTGGGTAGCTCTGGATCCGCGTACCACCGCTTGTTAGCGGTGGGATTTTTGGTTTGCCAAGCGCAGCAGAATATAATCA
[Summary]
Gray : vectors matched to NCBI univec sequence database
(1) Cloning vector pBR322
(2) E.coli lactose operon with lacI
(3) Cloning vector pGEM-7Zf
Pink : Quality < 15(Low Quality)
Blue : Quality >= 15 (High Quality)
Quality Grade(Excellent Q15 >= 95% ,Good Q15 >= 75% ,Fail Q15 < 75%)
[Analysis]
Quality Grade : Good
High Quality(score>=15) : 83.23%
Insert / Total : 128 / 1270
------
HOS1-FLC-P2_M13F.ab1
TAGTGTCCATTTCTTGGGCGATTGGGCCGACGTCGCATGCTCCTCTAGACTCGAGGAATTCGGTACCCCAAATATCTGGCCCGACGAAGAAAAAGTAGATAGGCACAAAAAATAGAAAGAAATAAAGCGAGAAAAGGAAAAAAAAAAATAGAAAGAGAAAACGCTTAGTATCTCCGGCGACTTGAACCCAAACCTGAGGATCAAATTAGGGCACAAAGCCCTCTCGGAGAGAAGCCATGGGAAGGGGTTCGAAATCGATAAGCTTGGATCCGGAGAGCTCCCAACGCGTTGGATGCATAGCTTGAGTATTCTATAGTGTCACCTAAATAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATATGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGACGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGACCCCCACGATCAGCCCGACCGCTGCGCTTATCCGGTACTATCGTCTGAGTCGACCCGGTAGACACGACTATCGCACTGCAGCAGCACTGTACAGGATAAGCTATCGAGGTATGTAGCGTGCTACGGTCTGATTGTGGACTAACTACGCTACCTAACGAACGTATCGTTCTGCCCTGCTAGCAGTTACTTCGCAAGATGAGCCTGATCCGGACCAGCACCATGGTAACGGTGATCTGATGCAGGCAGCGCAATATAC
[Summary]
Gray : vectors matched to NCBI univec sequence database
(1) Cloning vector pBR322
(2) E.coli lactose operon with lacI
(3) Cloning vector pGEM-7Zf
Pink : Quality < 15(Low Quality)
Blue : Quality >= 15 (High Quality)
Quality Grade(Excellent Q15 >= 95% ,Good Q15 >= 75% ,Fail Q15 < 75%)
[Analysis]
Quality Grade : Good
High Quality(score>=15) : 78.02%
Insert / Total : 175 / 1265
------
>HOS1-FLC-P3_M13R.ab1
GGGGGGCTCCTATTTTTGGGTGAACTATAGAATACTCAAGCTATGCATCCAACGCGTTGGGAGCTCTCCGGATCCAAGCTTATCGATTTCGAACCCTGTAAGCCACATTAATTGGGAAACTATGACTAAAAGATGAATTGGTAATATATACATAATATTTTTAACGAATTTCTCTCCTTTTTATGGGATATGCTATTTAAAGATTGTCCAAAGGTTTATAGTTTCCCACTCTTGCAGTTACACACAGGGGTACCGAATTCCTCGAGTCTAGAGGAGCATGCGACGTCGGGCCCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGCATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTTCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATCAAGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGAAAATAACCCTTGAAAATGCTTCATAATATTGAAAAAGGAGAGGTATGAGTTATTCAACATTTCCGTGTTCGCCCTTTATCCCTTTTTTGCGGCATTTTGCTTCAGTTTGCTCCACCCAGAACGCTGTGAAAGTAAAAGATGCTGAGAATCGTTGTGCCGAGTGGATTACATCGACCTGGATTCTCAACAAGCGGATAGATTCCTTGTGAAGGATTTTTCGCCCCCGA
[Summary]
Gray : vectors matched to NCBI univec sequence database
(1) Cloning vector pJAZZ-KA
(2) Cloning vector pBR322
(3) Phage M13 genome
(4) Synthetic construct CaMV 35S promoter and multiple cloning site
(5) E.coli lactose operon with lacI
(6) Cloning vector pGEM-7Zf
Pink : Quality < 15(Low Quality)
Blue : Quality >= 15 (High Quality)
Quality Grade(Excellent Q15 >= 95% ,Good Q15 >= 75% ,Fail Q15 < 75%)
[Analysis]
Quality Grade : Good
High Quality(score>=15) : 83.96%
Insert / Total : 150 / 1303
------
HDA6-FLC-P1_M13F.ab1
GGGGTTTACCTTTCGGGCGATTGGGCCCGACGTCGCATGCTCCTCTAGACTCGAGGAATTCGGTACCCCGCATCAAAACTTCTTGGCACAGCTCCGAGTGTTACTGAAATGTTTGTGTGGCTCCAATAGAAAAGTTAATACCAATCATGAATCATCAATATCGTTACAAAATCAGTAATCAGTTTCACCCACTCCGAGGGGTTCGAAATCGATAAGCTTGGATCCGGAGAGCTCCCAACGCGTTGGATGCATAGCTTGAGTATTCTATAGTGTCACCTAAATAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTTACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACGAGTTCTTTGAAGTGGTGGCCTACTACGGCTACCCTAGAAGAACAGTATTTGGTATCTGCCCTCTGCTGAG
CCAGTAACTCCGAAAGAGTTGTAGCTCTGTATTCGCAACAACACCGCTGTACGGTGTTTTTTGTGCAGCGCAGAATCCGGCCGAATAAGAATCCTCAGAAGATCCTTGAACTCTTTCTACGGGGGTC
[Summary]
Gray : vectors matched to NCBI univec sequence database
(1) Cloning vector pEZSeq-Kan
(2) Cloning vector pBR322
(3) E.coli lactose operon with lacI
(4) Cloning vector pGEM-7Zf
Pink : Quality < 15(Low Quality)
Blue : Quality >= 15 (High Quality)
Quality Grade(Excellent Q15 >= 95% ,Good Q15 >= 75% ,Fail Q15 < 75%)
[Analysis]
Quality Grade : Good
High Quality(score>=15) : 83.42%
Insert / Total : 128 / 1297
------
>HDA15-FLC-P1_M13R.ab1
CGAACCTTTTTCGTGCCTATAGAATACTCAAGCTATGCATCCAACGCGTTGGGAGCTCTCCGGATCCAAGCTTATCGATTTCGAACCCGCATCAAAACTTCTTGGCACAGCTCCGAGTGTTACTGAAATGTTTGTGTGGCTCCAATAGAAAAGTTAATACCAATCATGAATCATCAATATCGTTACAAAATCAGTAATCAGTTTCACCCACTCCGAGGGGTACCGAATTCCTCGAGTCTAGAGGAGCATGCGACGTCGGGCCCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTTCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATCAGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATCACATTTCCGTGTCGCCCTTATCCCTTTTTTGCGCATTTGCTCTGTTTTTGCTCACCCAGAAACGCTGTGAAGTAAAAGAATGCTGAGATCAGTTGGGTGCACGAGTGGATTAATCGAACTGGAATCCCACAGCGTTAGAATCTGGAGAAGCTTTCGCCCGCAGAACGGTTTCCAAGGGAGTGAAACT
[Summary]
Gray : vectors matched to NCBI univec sequence database
(1) Cloning vector pJAZZ-KA
(2) Cloning vector pBR322
(3) Phage M13 genome
(4) Synthetic construct CaMV 35S promoter and multiple cloning site
(5) E.coli lactose operon with lacI
(6) Cloning vector pGEM-7Zf
Pink : Quality < 15(Low Quality)
Blue : Quality >= 15 (High Quality)
[Analysis]
Quality Grade : Good
High Quality(score>=15) : 86.6%
Insert / Total : 127 / 1284
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