Supplemental Data. Peterson et al. (2010). Plant Cell 10.1105/tpc.109.072777
Supplemental Dataset 3. Amino acid alignment of the N terminal end of YODA and related genes presented in Figure 6C. Sequence alignment is of the N terminal end, including the regulatory region unique to YODA (yellow; Figure 4C) though the conserved kinase domain (gray). YODA is a member of the A2 clade of MAPK kinase kinase proteins in Arabidopsis (Ichimura et al. 2002). This clade also contains At1g53570 and At1g66850. A3 is the clade sister to clade A2 in the MKKK-wide phylogeny, and it is composed of At1g09000, At1g54960, and At3g06030 (Ichimura et al. 2002). None of the Arabidopsis genes closely related to YODA contain complete sequence in the YODA N terminal regulatory domain.
At_YODA -MPWWSKS--KDEKKK------TNKES-IID--AFNRKLGFASEDRSSGRSRK
Os_YODA1 MPPWWGKSFSKDAKK------TTKEN-LID--TFHRLIS-PNDQKGSTKSKR
Os_YODA2 MPPWWGKSSSKEVKK------TAKEN-LID--TFHRLLS-PNEQKGRTKSRG
Pt_YODA1 ------
Pt_YODA2 MRSWWGKSSSKEEKKK------ANKES-FID--TINRKFKITSKEKSNNRSGG
Pp_YODA -MPWWPISSKDSKKGK------KPTTSGLFDGGSGHSRSPKPRAKEDNGSSKC
At1g53570 MPTWWGRKSCKNKDD------NHRGIISTDRDIKSSAVVVDPPLTPTRGG
At5g66850 -MRWLPQISFSSPSSSPSSSLKPVASYSESPDPDRNQDRDRFHRRLFRFNRGRLTRQRKL
At1g09000 ------
At1g54960 ------
At3g06030 ------
At_YODA SRRRRDEIVSERGAISRLPSRSPSPSTRVSRCQSF-AERSPAVPLPRPIVRP---HVTST
Os_YODA1 SCRRGNDSSVEKSCRSTTVSRPTSPSKEVSRCQSFSADRPHAHPLPIPGVRP---PVTRT
Os_YODA2 NRRHSKDPTAEKGCWSTAQSRSASPSKEVSRCQSFAAARAHAQPLPLPRSRA---MVART
Pt_YODA1 ------
Pt_YODA2 SRRCCKDTLSERVSLSRVPSRSPSPSTHVSRCQSF-AERPQAQPLPLPLPGVPHTKIGRC
Pp_YODA NSPYSLDLASEAGSGSGVGSRSPSPSSPIHNSKRR---HSQGQPLPLPCAPPTKSVGRAY
At1g53570 TPRCSREFAGASSAFSGFDSDSTE------KKGHPLPRPLLSP------
At5g66850 RHLTDDDVLLGERRASTSSSTFDSGLTRSPSAFTAVPRSPSAVPLPLPLPLP------
At1g09000 ------
At1g54960 ------
At3g06030 ------
At_YODA DSGMNGSQRPGLDANLKP-SWLPLPKPHGATSIPDNTGAEPDFATASVSSGSSVG--DIP
Os_YODA1 VSDITES-KPILEKRGKPPLLLPLPKPNRPPRRHGNSEVVSEIVVASPSSN-CSDSDDHG
Os_YODA2 ASDITES-KVVLEKRGKG-QQLPLPTTNWVKERPETTEPVAELSTASISSHGSIDSDDPG
Pt_YODA1 ------
Pt_YODA2 DSGISASVKPGLDGGGKPLHLLPLPRPGHVLNRLDQADTAGDLATASVSSDSSIDSDDLP
Pp_YODA SEVQLPNVQNQWGSLLHHAASMPLPSPAQVGLMDNGDYPSGSVSSASSLGSVEVDPRQQG
At1g53570 ------VSIHHQDHVSGSTSGSTSVSSVS-SSG------
At5g66850 ------EVAGIRNAANARGLDDRDRDPERLISDRTSSG------
At1g09000 ------
At1g54960 ------
At3g06030 ------
At_YODA SDSLLSPLAS--DCENGNRTPVN----ISSRDQS-MHSNKNSA-EMFKP--VPNKNRILS
Os_YODA1 DSQLQSPVGN--DAENATLVTLKNKSSNARKECPGPITAKNMK-EIHRPANQVHGSHILS
Os_YODA2 DLRLQGPVAN--DTDNVAKVATTGNSSVVHKECSSAITRKGTK-EVTMPTNAFLSNQILS
Pt_YODA1 ------MQQDQSPIVNKKNSI-ETLKPANLPVNNQILP
Pt_YODA2 DSRVLSPLTS--DYENGNRTAVNSPPSVMRQDQSPIINRKNSR-ETLKHANLPANNQTLS
Pp_YODA RASHLRPLQEPEPPEQLRPVPISRMSHVSAASRGGVLRHEPVRSENSRSTSLFPGSYPPV
At1g53570 ------SADDQSQLVASRGRG------DVKFNVA
At5g66850 ------PPLTSVNGGFARDSRK------ATENSSYQD
At1g09000 ------
At1g54960 ------
At3g06030 ------
At_YODA ASPRR-RPLGTHVKNLQIPQRDLVLCSAPDSLLSSPSRSPMRSFIPDQVSNHGLLISKPY
Os_YODA1 TSPRG-VAADSYQSNLQNPRP-LVLDSAPNSLMSSPSRSPRR-ICPDHIPTSAFWAVKPH
Os_YODA2 TSPRGTVVADSYQSNLQNSRK-VVLDSAPNSVMSSPSRSPRI-LCPDQIPSSAFWAVKPH
Pt_YODA1 TPPKR-AIFSSQVQNLQIPHR-GAFFSAPDSSLSSP-RSPMRAFGTEQVINNSFWTGKTY
Pt_YODA2 TPPKR-AIFSSQVQNLQIPHR-VAFFSAPDSSMSSPSRSPMRAFGTEQVINNGFWAGKTY
Pp_YODA TSPTSRVWPPKWPSPLKISQS------QPDSSKSSPVRSPRY------VSQHQRNGMEPT
At1g53570 AAPRS------PER----VSPKAATITTRPTSPRH------
At5g66850 FSPRNRNGYWVNIPTMSAPTS------PYMSPVPSPQRKSTGHDLPFFYLPPKSNQAWSA
At1g09000 ------MQDFFGSVRR------
At1g54960 ------
At3g06030 ------MQDILGSVRR------
At_YODA SDVSLLGSGQCSSPGSGYNSGNNSIGGDMATQLFWPQSRCSPECSPVPSPRMTSPGPSSR
Os_YODA1 TDVTFVGSGQCSSPGSGQTSGHNSVGGDMLAQLFWQPSRSSPECSPIPSPRMTSPGPSSR
Os_YODA2 TDVTFVGSAQCSSPGSGQTSGHNSVGGDMLAQLFWQPSRGSPECSPIPSPRMTSPGPSSR
Pt_YODA1 SDIGLLGSGQCSSPGSGYNSGQNSIGGDMSGQLLWPNSRCSPECSPLPSPRMTSPGPSSR
Pt_YODA2 SDIGLLGSGQCSSPGSGYNSGQNSIGGDMSGQLLWPNSRCSPECSPLPSPRVISPGPSSR
Pp_YODA VSGSVTG-----SPHSGNTSAYNSWQGDLAPLMAPQHHE---DRSLSPSPRLRSPGPSSR
At1g53570 ------QRLSGVVSLESSTG------RNDDGRSSSECHPLPRP-PTSPTSPSA
At5g66850 PDMPLDTSGLPPPAFYDITAFSTDNSPIHSPQPRSPRKQIRSPQPSRPSSPLHSVDSSAP
At1g09000 ------SLVFRPSSDDDNQENQPPFPGVLADKITSCIRKSKIFIKPSFSPPPPAN
At1g54960 ------MVFAKSQS------PPNNS
At3g06030 ------SLVFRSSLAGDDGTSGGGLSG-FVGKINSSIRSSRIGLFSKPPPGLPAP
At_YODA IQSGAVTPLHPRAGGSTTGSPTRRLDDNR-QQSHRLPLPPLLISNTCPFS-PTYSAATS-
Os_YODA1 VHSGSVSPLHPRSGGMAPESPTNRHDDGKKKQTHKLPLPPLSIS-HSSFH-PNNSTPTSP
Os_YODA2 VHSGSVSPLHPRAGGMAPESPTRRLDEGKRKQTHRLPLPPLSICNNSTFL-PNNSTPTSP
Pt_YODA1 IHSGAVTPLHHRAVGVTIESPTSCPDDGK-QQSHRLPLPPITTSNTCPFS-PTYSTTTS-
Pt_YODA2 IHSGAVTPLHPRAAGVTIESPTSRPDDGK-QQSHRLPLPPITISNTHPFS-PTYSASTS-
Pp_YODA TTSAAVSPLHPRAVNRTSGGP------LPLPPLPTGNSSPYTSPSTPTMSRH
At1g53570 VHGSRIG------
At5g66850 PRDSVSSPLHPRLSTDVTNGRRDCCNVHP------LPLPPGATCSSSSAA------S
At1g09000 TVD------
At1g54960 TVQ------
At3g06030 RKE------
At_YODA PSVPRSPARAEATVSPGSRWKKGRLLGMGSFGHVYLGFNSESGEMCAMKEVTLCS---DD
Os_YODA1 ISVPRSPGRTENPPSPGSRWKKGKLIGRGTFGHVYVGFNSDSGEMCAMKEVTLFL---DD
Os_YODA2 ISH--SPGRVENPTSPGSRWKKGKLVGRGTFGHVYIGFNSDKGEMCAMKEVTLFS---DD
Pt_YODA1 PSVPRSPNRMENPTSPGSRWKKGRLLGRGSFGDVYLGLNSESGELCTMKEVTLFS---DD
Pt_YODA2 PSVPRSPSRMENPTSSGTRWQKGRMLGRGSFGDVYLGFNRERGEMCAMKEVTLFS---DD
Pp_YODA LSSTLERSRSGESPVSSTKWQKGKLLGSGTFGNVYVGFNNDNGGFCAMKEVLLVS---DD
At1g53570 ------GGYETSPSGFSTWKKGKFLGSGTFGQVYLGFNSEKGKMCAIKEVKVIS---DD
At5g66850 VPSPQAPLKLDSFPMN-SQWKKGKLIGRGTFGSVYVASNSETGALCAMKEVELFP---DD
At1g09000 ------MAPPISWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLIAANFASK
At1g54960 ------IKPPIRWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLITSNCASK
At3g06030 ------EAPSIRWRKGELIGCGAFGRVYMGMNLDSGELLAIKQVLIAPSSASK
At_YODA PKSRESAQQLGQEISVLSRLRHQNIVQYYGSETVDDKLYIYLEYVSGGSIYKLLQEYG-Q
Os_YODA1 PKSKESAKQLGQEISLLSRLQHPNIVQYYGSETVDDKLYIYLEYVSGGSIHKLLQEYG-Q
Os_YODA2 PKSKESAKQLCQEILLLNRLQHPNIVRYYGSEMVDDKLYIYLEYVSGGSIHKLLQEYG-Q
Pt_YODA1 AKSKESAQQLGQEIMLLSRLRHPNIVQYYGSETVEDKLYIYLEYVSGGSIYKLLQEYG-Q
Pt_YODA2 AKSKESAQQLGQEIGLLSRLRHPNIVQYYGSETVDDKLYIYLEYVSGGSIYKLLQEYG-Q
Pp_YODA HKSKESVKQLGQEISLLSKLRHENIVQYIGTETLEDRLYIYLEYVSGGSIHKLLQEYG-A
At1g53570 QTSKECLKQLNQEINLLNQLCHPNIVQYYGSELSEETLSVYLEYVSGGSIHKLLKDYG-S
At5g66850 PKSAECIKQLEQEIKLLSNLQHPNIVQYFGSETVEDRFFIYLEYVHPGSINKYIRDHCGT
At1g09000 EKTQAHIQELEEEVKLLKNLSHPNIVRYLGTVREDDTLNILLEFVPGGSISSLLEKFG-P
At1g54960 EKTQAHIQELEEEVKLLKNLSHPNIVRYLGTVREDETLNILLEFVPGGSISSLLEKFG-A
At3g06030 EKTQGHIRELEEEVQLLKNLSHPNIVRYLGTVRESDSLNILMEFVPGGSISSLLEKFG-S
At_YODA FGENAIRNYTQQILSGLAYLHAKNTVHRDIKGANILVDPHGRVKVADFGMAKHITAQSGP
Os_YODA1 LGEQAIRSYTQQILSGLAYLHAKNTVHRDIKGANILVDPSGRVKLADFGMAKHINGQQCP
Os_YODA2 FGEPAIRSYTKQILLGLAYLHAKNTVHRDIKGANILVDPNGRVKLADFGMAKHINGQQCA
Pt_YODA1 FGEIAIRSYTQQILSGLAYLHAKKTVHRDIKGANILVDPTGRVKLADFGMAKHISGQSCP
Pt_YODA2 FGEIAIRSYTQQILRGLAYLHAKKTVHRDIKGANILVDPTGRVKLADFGMAKHISGQSCP
Pp_YODA FKEPVVRNYTRQILSGLAYLHNQNTVHRDIKGANILVDTNGMVKLADFGMAKHISAQSFL
At1g53570 FTEPVIQNYTRQILAGLAYLHGRNTVHRDIKGANILVDPNGEIKLADFGMAKHVTAFSTM
At5g66850 MTESVVRNFTRHILSGLAYLHNKKTVHRDIKGANLLVDASGVVKLADFGMAKHLTGQRAD
At1g09000 FPESVVRTYTRQLLLGLEYLHNHAIMHRDIKGANILVDNKGCIKLADFGASKQVAELATM
At1g54960 FPESVVRTYTNQLLLGLEYLHNHAIMHRDIKGANILVDNQGCIKLADFGASKQVAELATI
At3g06030 FPEPVIIMYTKQLLLGLEYLHNNGIMHRDIKGANILVDNKGCIRLADFGASKKVVELATV
At_YODA ---LSFKGSPYWMAPEV-----IKNSN-GSNLAVDIWSLGCTVLEMATTKPPWS-QYEGV
Os_YODA1 ---FSFKGSPYWMAPEV-----IKNSN-GCNLAVDIWSLGCTVLEMATSKPPWS-QYEGI
Os_YODA2 ---FSFKGSPYWMAPEV-----IKNSN-GCNLAVDIWSLGCTVLEMATSKPPWS-QYEGI
Pt_YODA1 ---FSFRGSPYWMAPEV-----IKNSN-GCNLAVDIWSLGCTVLEMATTKPPWS-QYEGV
Pt_YODA2 ---LSFKGSPYWMAPEV-----IKNSN-GCNLAVDIWSLGCTVLEMATTKPPWS-QYEGV
Pp_YODA ---QSFKGSPYWMAPEVRVNCIITSTD-WYDLAVDIWSLGCTVLEMLTTKPPWN-QYEGV
At1g53570 ---LSFKGSPYWMAPEV-----VMSQN-GYTHAVDIWSLGCTILEMATSKPPWS-QFEGV
At5g66850 ---LSLKGSPYWMAPELMQAVMQKDSNPDLAFAVDIWSLGCTIIEMFTGKPPWS-EFEGA
At1g09000 TGAKSMKGTPYWMAPEV-----ILQTG--HSFSADIWSVGCTVIEMVTGKAPWSQQYKEV
At1g54960 SGAKSMKGTPYWMAPEV-----ILQTG--HSFSADIWSVGCTVIEMVTGKAPWSQQYKEI
At3g06030 NGAKSMKGTPYWMAPEV-----ILQTG--HSFSADIWSVGCTVIEMATGKPPWSEQYQQF
At_YODA PAMFKIGNSKELPDIPDHLSEEGKDFVRKCLQRNPANRPTAAQLLDHAFV
Os_YODA1 AAMFKIGNSKELPPIPDHLSEPGKDFIRKCLQRDPSQRPTAMELLQHPFV
Os_YODA2 AAVFKIGNSKELPPIPDHLSEEGRDFIRQCLQRNPSSRPTAVDLLQHSFI
Pt_YODA1 PAMFKIGNSKELPEIPDHLSDDGKDFVRQCLQRNPSHRPTAAQLLDHPFV
Pt_YODA2 PAMFKIGNSKELPEIPDNLSDDGKDFVRQCLQRNLSHRPTAAQLLEHPFV
Pp_YODA AAMFKIGNSKELPVIPNTLSRTGREFVRLCLQRDPAQRPTAAQLLEHPFV
At1g53570 AAIFKIGNSKDTPEIPDHLSNDAKNFIRLCLQRNPTVRPTASQLLEHPFL
At5g66850 AAMFKVMR--DSPPIPESMSPEGKDFLRLCFQRNPAERPTASMLLEHRFL
At1g09000 AAIFFIGTTKSHPPIPDTLSSDAKDFLLKCLQEVPNLRPTASELLKHPFV
At1g54960 AAIFHIGTTKSHPPIPDNISSDANDFLLKCLQQEPNLRPTASELLKHPFV
At3g06030 AAVLHIGRTKAHPPIPEDLSPEAKDFLMKCLHKEPSLRLSATELLQHPFV