Supplemental Data. Liwanag et al. (2012). Plant Cell 10.1105/tpc.112.106625

Arabidopsis_thaliana_At2g33570_GALS1 MR------KEVLPP----VLSTTTVCFEKKPIIATLLALSLVMIVWNLPPYYHN------LIS-TARPCSAVTTTTTTTLLSSSNFTSAENFTTSLSTTT 83

Arabidopsis_thaliana_At5g44670_GALS2 ------MAKERDQNTKDKNLLICFLWNFSAELKLALMALLVLCTLATLLPFLPS------SFSISASELRFCISRIAVNSTSVNFTTVVEKPVLDNAVKL 88

Arabidopsis_thaliana_At4g20170_GALS3 ----MAMVKEKEQNTKDKKLLVGVIWNFSAELKLTFMALLVLCTLATLLPFIPS------SFSLSTSDFRFCISRFSS-AVPLNTTTTVEE---SSSSPS 86

Caenorhabditis_elegans_CCD64148 ------MPRITASKIVLLIALSFCITVIYHFPIATRSSKE------YDEYGNEYENVASIESDIKNVRRLLDEVPDP------65

Columba_livia_ADC84389 ------MIHCGKKHICAFVTCILISASILMYSWKDPQLQN------NITRKIFQATSALPASQLCRGKPAQN------60

Oryza_sativa_Os06g0624700 MR-KDAAAGGGGGGGIAAGPAPALLCFDLKPFLAALTVLTLAVRTVASSGN------ASVSSDPG----GPPASLPEVGNKK------P 72

Oryza_sativa_Os02g0712500 MK--NGARKDAGGGG----GGVFVPCVDIKSFVLSLAFLTVFVALWQLQPYGSLLTAARSTASVSASPCSLIATPAAAAASAGVIRSENSTADT-AKTAP 93

Oryza_sativa_Os06g0328800 MKPAGGRSKDGGGGGAALLGGGGVTCFDVKSFVASLALLTLIMALWQLHPYQPLVLLP---AALSSSPCPLLPRSPTSGIAVSFLSTAAATNSTDTATVP 97

Populus_trichocarpa_1066369 ------

Populus_trichocarpa_712653 ------

Populus_trichocarpa_715742 MR------KDCQPPSSGATVSKLPACFETKPLVATLLALTLVMLLWNLPPYYQN------LLSTTTRPSCSAPETTVSISNTSSSFTS-----TSLSDQK 83

Selaginella_moellendorffii_XP_002965483 ----MAAFHKAMICRIWIVLLVTLLWIGLWYSRISPATSDLAAHRKDLHRFGGN-----LARNSSFSKFWEVEDGQLTADLIHEVVEELEKIRDGTPGGV 91

Selaginella_moellendorffii_XP_002961724 ------MPITQSCENSSSNLS------VSTNFGPAAAIDPIPAPEEQGTGIHS------41

1...... 10...... 20...... 30...... 40...... 50...... 60...... 70...... 80...... 90...... 100

Arabidopsis_thaliana_At2g33570_GALS1 AAASQKYDSTPS------DPNKRVFQPFGNAAALFVLMGAYRGGPTTFSVIGLASKPIHVYG-KPWYKCEWISNN----GTS----IRAK-AQKI 162

Arabidopsis_thaliana_At5g44670_GALS2 TEKPVLDNGVTKQPLTEEKVLNNGVIKRTFTGYGWAAYNFVLMNAYRGGVNTFAVIGLSSKPLHVYS-HPTYRCEWIPLNQ--SDNR-----ILTDGTKI 180

Arabidopsis_thaliana_At4g20170_GALS3 PEK------NLDRVLDNGVIKRTFTGYGSAAYNFVSMSAYRGGVNSFAVIGLSSKPLHVYG-HPSYRCEWVSLDP--TQDP-----ISTTGFKI 166

Caenorhabditis_elegans_CCD64148 ------SQNRLQFLKLDEHAFAFSAYTDDRNGNMGYKYVRVLMFITSQDN----FSCEINGRKS------TDVSLYEF 127

Columba_livia_ADC84389 ------VITALEDNRTFIISPYFDDRESKVTRVIGIVHHEDVKQLYCWFCCQPDGKIY------VARAKIDVH 121

Oryza_sativa_Os06g0624700 VAAAAAA------DPNRREFRAVGSAAALFVQMGAYRGGPYTFAVIGLASKPTHVYG-KPWFRCEWVPNANGSAAAAAARPMRAANTYHM 155

Oryza_sativa_Os02g0712500 AAVASAVPARLARAARPARVE--DPNKRELRPYGSAAALFVQMGAYRGGPRTFAIVGLASKPTHVFS-NPYFKCEWLPNP--TAGNPSPRPVRTK-AYKM 187

Oryza_sativa_Os06g0328800 TTTAAARVAATTRPTLPARQRERDPNKRELRPYGTAAALFVQMGAYRGGPRTFAVVGLASKPAHVFS-NPYFKCEWLPNA--PAGAP---PVRTK-AYKM 190

Populus_trichocarpa_1066369 ------MGAYRGGLNTFAIIGLASKPLHVYS-KPAYQCEWVPQSSSASNST-----FSTVSYKM 52

Populus_trichocarpa_712653 ------MGAYRGGHNTFAIIGLASKPLLLYS-TPTYQCEWVPESSSASNST-----FSTVSYRI 52

Populus_trichocarpa_715742 YLSSSSSSSSNA------DPNKRIFQAYGNAAALFVQMGAYRGGPTTFAVVGLASKPIHVFR-LPWYKCEWISNN----GSS----IRAK-AYKM 162

Selaginella_moellendorffii_XP_002965483 VAANRKEPCDERR------DLARERGFRRIGETLHAFVLSSVARFSLEEFTAVGLVSIQLRGFAGNPLQSCAWVPKAGSSSVAAALAASNDSSVIWG 182

Selaginella_moellendorffii_XP_002961724 ------TVRHLHPFGIASYTFVLTGGYRTQRRSFAVVGLAAKSLHVFG-KPNFACEWVPDS----TTSDSPSIFGS-ARKI 110

...... 110...... 120...... 130...... 140...... 150...... 160...... 170...... 180...... 190...... 200

Arabidopsis_thaliana_At2g33570_GALS1 LPDWGYG------RVYTVVVVNCTFN--SNPNSDNTGGKLILNAYY------NESPKLFERFTTLEESAGIYDESKYSPP----YQYDYLYCGSSL 240

Arabidopsis_thaliana_At5g44670_GALS2 LTDWGYG------RVYTTVVVNCTFPSNTVINPKNTGGTLLLHATT------GDTDRNITDSIPVLTETPNTVDFALYESNLRRREKYDYLYCGSSL 265

Arabidopsis_thaliana_At4g20170_GALS3 LTDWGYG------RIYTTVVVNCTFSSISAVNPQNSGGTLILHATT------GDPTLNLTDSISVLTEPPKSVDFDLYNS-TKKTKKYDYLYCGSSL 250

Caenorhabditis_elegans_CCD64148 SENHKMK------WQMFILNCKLP--DGIDFNNVSSVKVIRSTT------KQFVDVPIRYRIQDEKIITPD---EYDYKMSICVPAL 197

Columba_livia_ADC84389 SDRFGFP------YGAADIVCLEP--ENCNPTHVSIHQSPHANI------DQLPSFKIKNRKSET------FSVDFTVCISAM 184

Oryza_sativa_Os06g0624700 LPDWGYG------RVYTVVVVNCTFA--RVPNADNAGGKLVLNAYY------GASPARYERIVAMEEAPGAYDAAEFRPP----HRYDYLYCGSSL 233

Oryza_sativa_Os02g0712500 LPDWGYG------RVYTVVVVNCTFP--SNPNADNAGGKLLVHAYY------STTSRRYERFVALEEAPGSYDESRFSPP----FPYDYLYCGSSL 265

Oryza_sativa_Os06g0328800 LPDWGYG------RVYTVVVVNCTFP--SNPNADNLGGKLLVHAYY------STASRRYERFVALEEAPGSYDDARFRPP----FAYDYLYCGSSL 268

Populus_trichocarpa_1066369 LPDWGYG------HVYTVVVVNCTFS--EAVNSENSGGKLFLEASTSGG-----GDRDFNITDRFEVLNESPGDINTTLFSS----KPKYDYLYCGSSL 134

Populus_trichocarpa_712653 LPDWGYG------RVYTVVVVNCTFS--EAVNGENSGGKLFLEASTSGG-----GDKNLNITDRFEVLNESPGDLNMSLFSS----KPKYDYLYCGSPL 134

Populus_trichocarpa_715742 LPDWGYG------RVYTVVVVNCTFP--VNPNQDNAGGRLMLNAYY------DESQRKYEKFMALEELPGSYNESKFRPP----YQYEYLYCGSSL 240

Selaginella_moellendorffii_XP_002965483 NSWLGYVNESHTEKTFYDVGVVKCSFE--EPVGANGEGGFLVLNMTRNSSPPPLVDHSGIIEQSYVPVFQELPEEISSIQFEGP----FRFDYAYCSPLL 276

Selaginella_moellendorffii_XP_002961724 LPDWGYG------RVYTVVVVHCYFK--SSVGEDGSGGRLILHAGE------KGSPSPPSKITALVEAPGSYSPSQFLP------KYDYLYCGSSL 186

...... 210...... 220...... 230...... 240...... 250...... 260...... 270...... 280...... 290...... 300

Arabidopsis_thaliana_At2g33570_GALS1 YG------NVSASRMREWMAYHAWFFGD-KSHFVFHDAGGVSP-EVRKVLEPWIRAGRVTVQNIRDQ-SQYDG-YYYNQFLIVNDCLHRYRYAANWTFF 329

Arabidopsis_thaliana_At5g44670_GALS2 YG------NLSPQRIREWIAYHVRFFGE-RSHFVLHDAGGITE-EVFEVLKPWIELGRVTVHDIREQ-ERFDG-YYHNQFMVVNDCLHRYRFMAKWMFF 354

Arabidopsis_thaliana_At4g20170_GALS3 YG------NLSPQRVREWIAYHVRFFGE-RSHFVLHDAGGIHE-EVFEVLKPWIELGRVTLHDIRDQ-ERFDG-YYHNQFMIVNDCLHRYRFMTKWMFF 339

Caenorhabditis_elegans_CCD64148 FG-N-----GYDAKRIVEFIELNT--LQGIEKIYIYTNQKELDG-SMKKTLKYYSDNHKITLIDYTLP-FREDGVWYHGQLATVTDCLLRNTGITKYTFF 287

Columba_livia_ADC84389 FGNYNNVLQFIQSVEMYKILGVQKVVIYKNNCSQLMEKVLKFYMEEGTVEIIPWPINSHLKVSTKWHFSMDAKDIGYYGQITALNDCIYRNMQRSKFVVL 284

Oryza_sativa_Os06g0624700 YG------NLSAARVREWMAYHARFFGL-RSHFVFHDAGGVSP-AVRAALEPWVRAGRATLQDVRAQ-AEYDG-WYYNQFLVVNDCLHRYRHAARWTFF 322

Oryza_sativa_Os02g0712500 YG------NLSASRMREWVAYHAHFFGP-RSHFVFHDAGGISP-EVKAVLDPWVRAGRLTVQDIRAQ-AEYDG-YYYNQFLVVNDCLHRYRHAANWTFF 354

Oryza_sativa_Os06g0328800 YG------NLSSARMREWLAYHARFFGP-RSHFVLHDAGGVTP-EVRAVLDPWVSAGRVTVQDIRAQ-EDYDG-YYYNQFLVVNDCLHRYRHAANWTFF 357

Populus_trichocarpa_1066369 YG------GLSPQRVREWIAYHVRLFGK-RSHFVIHDAGGVHE-EVLEVLKPWMELGYVTLQDIKEQ-ERFDG-YYHNQFMVVNDCLHRYKFMAKWMFF 223

Populus_trichocarpa_712653 YG------GLSPQRVREWIAYHVRLFGE-RSHFVIHDAGGVHE-EVLEVLKPWMELGYVTLQDVKEQ-ERFDG-YYHNQFMVVNDCLHRYKFMAKWMFF 223

Populus_trichocarpa_715742 YG------NLSASRFREWMAYHAWFFGP-SSHFVFHDAGGVSP-EVRAALDPWVRAGRATVQDIRGQ-AEFDG-YYYNQFLVVNDCLHRYRYSANWTFY 329

Selaginella_moellendorffii_XP_002965483 TPSK-----NVSAKYVKEWLMYHQGLWSESHVHYFFYDAGGIDS-RLLEVFQPLMEKKFLTVIDMREL-VLFES-AGDGHQLVINDCLQRSRFLATWAFF 368

Selaginella_moellendorffii_XP_002961724 FG------DVNPQRLREWIAYHAWLFGP-RSHFVLHDAGGARSKGVRLVLEPWVRSGRVTVQDFREQ-ARFDG-YYHNQFLVVNDCLFRYKHAANWTFF 276

...... 310...... 320...... 330...... 340...... 350...... 360...... 370...... 380...... 390...... 400

Arabidopsis_thaliana_At2g33570_GALS1 FDVDEYIYLPHGNTLESVLDEF----SVNTQFTIEQNPMSSVLCINDSSQ---DYPRQWGFEKLLFK------DSRTKIRRDRKYAIQAKNAFA 410

Arabidopsis_thaliana_At5g44670_GALS2 FDVDEFIYVPAKSSISSVMVSL----EEYSQFTIEQMPMSSQLCYDGDGP--ARTYRKWGFEKLAYR------DVKKVPRRDRKYAVQPRNVFA 436

Arabidopsis_thaliana_At4g20170_GALS3 FDVDEFLHVPVKETISSVMESL----EEYSQFTIEQMPMSSRICYSGDGP--ARTYRKWGIEKLAYR------DVKKVPRRDRKYAVQPENVFA 421

Caenorhabditis_elegans_CCD64148 NDFDEFFVPVIKS--RTLFETIS---GLFEDPTIGSQRTALKYINAKIKS------APYSLKNIVSE------KRIETRFTKCVVRPEMVFE 362

Columba_livia_ADC84389 NDADEIILPLKHLDWKAMMSSLQEQNPGAGIFLFENHIFPKTVSTPVFNISSWNRVPGVNILQHVHR------EPDRKEVFNPKKMIIDPRQVVQ 373

Oryza_sativa_Os06g0624700 FDVDEYIFLPDGRSLEAVLAEL----EPYTQFTIEQNPMSSRLCARNPDEPETDYSNEWGFEKLVFR------NSITGVRRDRKYAIQAKNAYA 406

Oryza_sativa_Os02g0712500 FDVDEYIYLPNGQTLDQVLGKL----SGYSQFTIEQNPMSSKLCVQDPSK---DYSREWGFEKLVFR------NSITKVRRDRKYAIQARNAYS 435

Oryza_sativa_Os06g0328800 FDVDEYIYLPDGRALEDVLAQL----QPYTQFTIEQNPMSSKLCIDDPTE---DYSREWGFEKLVFR------NSITGVRRDRKYAIQARNAYS 438

Populus_trichocarpa_1066369 FDVDEYIHVPQKNTIKSVLDSL----SDYTQFTIEQMPMNNKLCLSAD-Y--GRYYRKWGFEKLVYK------DVKRGIRRDRKYAIQPRNVFA 304

Populus_trichocarpa_712653 FDVDEYIYLPPKNTIKSVLDSL----SGYTQFTFEQMPMSSKLCLSAD-Y--GRYYRKWGIEKLVYR------DVKRGIRRDRKYAIQPRNVFA 304

Populus_trichocarpa_715742 FDVDEYIYLPEGNTLESVLKDF----SNYTQFTIEQNPMSSALCFNDSTQ---DYPRQWGFEKLLFR------ESRTGIRRDRKYAIQAKNAYA 410

Selaginella_moellendorffii_XP_002965483 WDLDEYLQLVNSTSMAALLRSY----ESSPWISLANLVWSRTYCKETSAE------DEWAVERMRFRLRYPTCGDEQRAETCHGVEGHRKWIANPRKVLV 458

Selaginella_moellendorffii_XP_002961724 FDVDEYVHVPGPSTLDSVLNELG---QKYSQIIFKQTPMGHNVCIDSNRT---NLSRQWGFEKLVFI------NVQKSVRLDRKYVLRAPLADS 358

...... 410...... 420...... 430...... 440...... 450...... 460...... 470...... 480...... 490...... 500

Arabidopsis_thaliana_At2g33570_GALS1 TGVHMSENIVGK---TLHKTETKIRYYHYHNTITVHEELCREMLPNSAK-KKVTLYNKLPYVYDDNMKKLVKTIKEFEQKKLGTDVKNFS- 496

Arabidopsis_thaliana_At5g44670_GALS2 TGVHMSQHLQGK---TYHRAEGKIRYFHYHGSISQRREPCRHLYN-----GTRIVHENNPYVLDTTMRDIGLAVKTFEIRTIGDRLLRTRQ 519

Arabidopsis_thaliana_At4g20170_GALS3 TGVHMSQNLQGK---TYHKAESKIRYFHYHGSISQRREPCRQLFN-----DSRVVFENTPYVLDTTICDVGLAVRTFELRTIGDRLLRTRQ 504

Caenorhabditis_elegans_CCD64148 QGIHHTSRVIQDNYKTVSHGGSLLRVYHYKDKKYCCEDES------LLKKRHGDQLREKFDSVVGLLDL------425

Columba_livia_ADC84389 TSVHSVLRAYGN---SVNVPADVALVYHCR--VPLQEELPRESLIR------DTALWRYNSSLITNVNKVLHQTVL------438

Oryza_sativa_Os06g0624700 TGVHMSENVIGN---TTHKTEHLIRYYHYHNTINVIGEVCREFVPIPPN-GGLIWSEKTPWYYDDSMKRIADTVREFERKTIG-DVRV--- 489

Oryza_sativa_Os02g0712500 AGVHMSQNVYGR---TTHKTESLIRYYHYHNSINVMGEPCREFVPVPVN-GSKLMFEGIPYVYDDNMKRLAGQIKRFEKEAIG-SAHT--- 518

Oryza_sativa_Os06g0328800 TGVHMSQNVYGR---TTHKTESLIRYYHYHNSINVMGEPCRKFVPKPAN-GSKVMFEGIPYVYDDNMKRLAGEIRRFEKQTIG-DVHT--- 521

Populus_trichocarpa_1066369 TGVHMSQNVAGK---TTHKTEGMIRYFHYHGTAAQRREPCRNLLN-----VTEINFENNPYVLDTTMRDLAWSVKKFENQMIGPKLKNTRQ 387

Populus_trichocarpa_712653 TGVHMSQNFAGK---TTYKTEGKIKYFHYHGTIAQRREPCRNLLN-----VTEINFENVPYVLDTTMRDLAWSVKKFELKTIGPKLQNTRQ 387

Populus_trichocarpa_715742 TGVHMSENVIGK---TLHQTETKIRYYHYHNSIQVPGELCREFLPLSAK-NNVTWYNGLPYVYDDNMKKLASTIKDFERNTIG-NVQAYS- 495

Selaginella_moellendorffii_XP_002965483 ASIHRTVEPEWN---GEILRASIARLNHFHGLAVEGSANSSCWIVK-----KLDQVNESSLVDGWWLKDESFAVAAMEQHRKGL------534

Selaginella_moellendorffii_XP_002961724 AGVHLSENVRGK---SLYS-RGQISYFHFHNTITEHQENCREFVKVPKNRTSKVWLNKVPYLYDDKLSTLADEVKQFELQTIG-PVTL--- 441

...... 510...... 520...... 530...... 540...... 550...... 560...... 570...... 580...... 590.