Supplement 1 – Supporting Tables and Figures
Table S1.1. Collecting sites for Lacertaagilisin Germany (Leipzig) and Bulgaria (Sofia) with associated patch sizes and number of sampled individuals.
Site Nr. / Site name / Number of individuals / Habitat patchsize in ha / Patch size classB1 / Petarch / 17 / 1595.69 / large
B2 / Simeonovo / 21 / 558.38 / medium
B3 / Mladost / 21 / 267.58 / medium
B4 / Lozen / 20 / 1064.82 / large
B5 / ElinPelin / 20 / 3111.75 / large
D1 / Wahren / 20 / 114.16 / small
D2 / Bienitz / 20 / 89.55 / small
D3 / Scherbelberg / 20 / 69.38 / small
D4 / Plautstraße / 20 / 18.67 / small
D5 / Lausen / 20 / 372.22 / medium
D6 / Antonienstraße / 19 / 30.68 / small
D7 / Am Panometer / 20 / 8.28 / small
D8 / Dölitz / 23 / 65.58 / small
D9 / Markkleeberg / 23 / 704.70 / medium
D10 / Trages / 22 / 856.32 / medium
D11 / Borna / 28 / 309.69 / medium
Table S1.2. Microsatellite primers published by Gullberg et al. (1997), with labelling name and annealing temperature (AT) used in the present study.
Name / Direction / Sequence / Motive / AT in °C / Accession numberLa-2 / Forward / FAM-GCTTAAATTGGAACCAGATTG / (GT/CA)16 / 64-54
Reverse / AAGCAGCCAGAACACAGAG
La-3 / Forward / FAM-ACTAGGAGCGAGAAGAATCAG / (GA/CT)28 / 60
Reverse / GACATATGGCAGAAGAGCAG
La-4 / Forward / FAM-CATGAGCAAAGCAATGAGC / (GT/CA)19 / 60
Reverse / TGGAATGTGTCATTGAACTCTG
La-6 / Forward / FAM-GACTGGCGCATTCTATAAAAC / (GT/CA)17 / 58
Reverse / GCCTTAAAGGGCCATCAG
La-9 / Forward / HEX-AGATGCTTTTATATATGCAACTTC / (GT/CA)12 / 54
Reverse / GTGCCTTCATTTGTTTACTTC
Table S1.3. Microsatellite primers published by Schwartz and Olsson (2008), with labelling name and annealing temperature (AT) used in the present study.
Name / Direction / Sequence / Motive / AT in °C / Accession numberLA01 / Forward / FAM-AACGGAGGTAGAATGTCATAGC / (GT)2AT(GT)15 / 56 / DQ860187
Reverse / CTTGAAGGGAAAGAGCTACTGC
LA02 / Forward / FAM-TGCCTGCAAGACTATAATCCAAG / (GT)23 / 56 / DQ60188
Reverse / GGAATGGCATGAGATATGGTG
LA3E / Forward / FAM-AAAGTTGGTCTGCACTGACG / (GT)13AT(GT)10 / 58 / DQ60189
Reverse / CAATTCAAAATGCACACAACG
LA04 / Forward / HEX-CTAGGCATGGAGAATGGATGTG / (CA)20 / 56 / DQ60190
Reverse / AGCCACTTCCCTAAGTGTGTCC
LA10 / Forward / FAM-TAATAAAGCAGGCGCAAACC / (CA)5(GA)4GGGA
(GACA)9(CA)9(GA)17 / 54 / EF028088
Reverse / TGCAGCTAATCTTCATTTAGGATG
LA12 / Forward / FAM-CAGAGTTCATGGAAAGTGAAGG / (CA)18 / 58 / DQ60191
Reverse / GGAGACTCTGCTGGTCATTC
LA27 / Forward / FAM-AAATGCAAGCGAGCAACAAT / (GT)11(AG)26 / 58 / DQ60192
Reverse / ATCTGGCGGAGGGATGAG
LA37 / Forward / HEX-TTTGCTTGGAGCTTCTGTCC / (GT)19 / 58 / DQ60194
Reverse / GATGCAGGACGGAGAGTAGC
LA40 / Forward / HEX-GGGAACCGTTGTACTAAGTTTGG / (CA)19 / 56 / DQ60195
Reverse / ATGCATTCAGATGTCTCCCAAG
LA45 / Forward / HEX-CAGAGTTCATGGAAAGTGAAGG / (CA)18 / 56 / DQ860196
Reverse / AAGGAGACTCTGCTGGTCATTC
LA47 / Forward / HEX-CCCACTAGAGAAATGAGCTTCTG / (GT)18 / 60 / DQ60197
Reverse / CAAACAAGGAGGGTAAGGAATG
LA50 / Forward / FAM-AGGTAGCCCAGGTGTCATACAG / (GT)21 / 60 / DQ60198
Reverse / TGGGTCTTACATGAGCTGAATC
LA55 / Forward / HEX-TCCCTCATTACAGGCATAGGAG / (CA)19 / 56 / DQ60199
Reverse / TCTGAACAAAACATGGGACTTG
LA58 / Forward / FAM-CAGTTCTGGGGATTTTCTCCTAC / (CA)18 / 56 / DQ60200
Reverse / CATTGTAATTGGAGCACAAAGC
LA64 / Forward / HEX-AGATGCTGAACTACCAGCTTGC / (CA)16 / 56 / DQ60201
Reverse / GCTATCCTGGCTGACCATTAAG
Table S1.4. Number of alleles per locus for each country.In red Loci that had to be excluded from further analyses (see main text).
Locus / Number of allelesBulgaria / Leipzig
La-1 / Illegible / illegible
La-2 / 11 / 9
La-3 / 13 / illegible
La-4 / 9 / 10
La-6 / 8 / 3
La-9 / 1 / 5
LA01 / 10 / 14
LA02 / 14 / 16
LA3E / 9 / 17
LA04 / 15 / 18
LA10 / 18 / illegible
LA12 / 14 / 10
LA27 / 17 / 23
LA40 / 12 / 11
LA45 / 14 / 10
LA47 / 10 / 12
LA50 / 6 / 12
LA55 / 7 / 11
LA58 / 12 / 10
LA64 / 4 / 9
Table S1.5. Genetic diversity parameters (mean ± 1 standard deviation) for the German and Bulgarian datasets; HO – observed heterozygosity, HE – expected heterozygosity, AR – allelic richness, AP – private allelic richness, FIS – heterozygote deficit.
Nr / Population / Ho / HE / AR / AP / FISBulgaria
B1 / Petarch / 0.63 ± 0.32 / 0.64 ± 0.27 / 6.75 ± 3.72 / 0.91 ± 0.83 / 0.07 ± 0.24
B2 / Simeonovo / 0.58 ± 0.27 / 0.62 ± 0.26 / 5.54 ± 2.69 / 0.49 ± 0.60 / 0.07 ± 0.26
B3 / Mladost / 0.59 ± 0.31 / 0.61 ± 0.30 / 5.92 ± 3.51 / 0.17 ± 0.32 / 0.03 ± 0.13
B4 / Lozen / 0.60 ± 0.32 / 0.61 ± 0.29 / 6.15 ± 3.44 / 0.53 ± 0.62 / 0.05 ± 0.24
B5 / ElinPelin / 0.62 ± 0.27 / 0.65 ± 0.26 / 6.55 ± 3.13 / 0.46 ± 0.58 / 0.05 ± 0.17
Germany
D1 / Wahren / 0.62 ± 0.21 / 0.65 ± 0.19 / 5.36 ± 2.52 / 0.25 ± 0.62 / 0.04 ± 0.17
D2 / Bienitz / 0.64 ± 0.23 / 0.64 ± 0.17 / 5.53 ± 1.94 / 0.16 ± 0.38 / -0.01 ± 0.23
D3 / Scherbelberg / 0.65 ± 0.20 / 0.68 ± 0.19 / 5.77 ± 2.10 / 0.41 ± 0.66 / 0.04 ± 0.11
D4 / Plautstraße / 0.54 ± 0.22 / 0.61 ± 0.23 / 5.20 ± 2.17 / 0 / 0.09 ± 0.21
D5 / Lausen / 0.63 ± 0.16 / 0.64 ± 0.16 / 5.52 ± 1.85 / 0.17 ± 0.38 / 0.01 ± 0.12
D6 / Antonienstraße / 0.67 ± 0.24 / 0.66 ± 0.22 / 6.08 ± 2.94 / 0.35 ± 0.48 / 0.01 ± 0.19
D7 / Am Panometer / 0.67 ± 0.23 / 0.66 ± 0.17 / 5.69 ± 2.85 / 0.12 ± 0.36 / 0.02 ± 0.16
D8 / Dölitz / 0.65 ± 0.11 / 0.68 ± 0.11 / 5.21 ± 1.74 / 0.32 ± 0.59 / 0.03 ± 0.15
D9 / Markkleeberg / 0.67 ± 0.16 / 0.69 ± 0.12 / 5.89 ± 2.21 / 0.22 ± 0.36 / 0.04 ± 0.15
D10 / Trages / 0.69 ± 0.17 / 0.71 ± 0.12 / 6.39 ± 2.45 / 0.15 ± 0.35 / 0.04 ± 0.13
D11 / Borna / 0.69 ± 0.12 / 0.72 ± 0.13 / 7.18 ± 3.04 / 0.41 ± 0.45 / 0.03 ± 0.09
Table S1.6. Pairwise genetic distances of all Bulgarian Lacertaagilis populations with FST values shown in the bottom half and corresponding geographic distances in km in the upper half.
B1 / B2 / B3 / B4 / B5B1 / — / 31.2 / 31.5 / 36.8 / 43
B2 / 0.032 / — / 3.3 / 9.2 / 19.6
B3 / 0.043 / 0.028 / — / 6 / 16.8
B4 / 0.045 / 0.032 / 0.036 / — / 11.2
B5 / 0.036 / 0.032 / 0.033 / 0.048 / —
Table S1.7. Pairwise genetic distances of all Bulgarian Lacertaagilis populations with DST values shown in the bottom half and corresponding geographic distances in km in the upper half.
B1 / B2 / B3 / B4 / B5B1 / — / 31.2 / 31.5 / 36.8 / 43
B2 / 0.117 / — / 3.3 / 9.2 / 19.6
B3 / 0.118 / 0.115 / — / 6 / 16.8
B4 / 0.125 / 0.123 / 0.104 / — / 11.2
B5 / 0.110 / 0.102 / 0.094 / 0.124 / —
Table S1.8. Pairwise genetic distances of all GermanLacertaagilis populations with FST values shown in the bottom half and corresponding geographic distances in km in the upper half.
D1 / D2 / D3 / D4 / D5 / D6 / D7 / D8 / D9 / D10 / D11D1 / — / 5.3 / 5.3 / 6.5 / 10.4 / 9.1 / 11.9 / 14.6 / 17.9 / 29.7 / 36.7
D2 / 0.178 / — / 6.2 / 3.2 / 5.3 / 6.1 / 10.6 / 13 / 15.2 / 26.9 / 32.9
D3 / 0.150 / 0.093 / — / 4 / 8.9 / 5.1 / 6.8 / 9.8 / 13.2 / 24.3 / 31.9
D4 / 0.160 / 0.121 / 0.119 / — / 4.9 / 3.1 / 7.5 / 9.6 / 12.1 / 23.9 / 30.5
D5 / 0.165 / 0.118 / 0.105 / 0.084 / — / 5.7 / 10.6 / 1.7 / 12 / 23.9 / 28.6
D6 / 0.134 / 0.128 / 0.118 / 0.051 / 0.061 / — / 5.0 / 6.7 / 9.1 / 20.9 / 27.2
D7 / 0.168 / 0.099 / 0.101 / 0.093 / 0.079 / 0.065 / — / 2.7 / 6.5 / 17.6 / 25.4
D8 / 0.160 / 0.110 / 0.124 / 0.104 / 0.108 / 0.115 / 0.091 / — / 4.2 / 14.9 / 22.8
D9 / 0.165 / 0.139 / 0.112 / 0.114 / 0.054 / 0.086 / 0.093 / 0.111 / — / 11.6 / 18.9
D10 / 0.151 / 0.138 / 0.088 / 0.084 / 0.088 / 0.083 / 0.093 / 0.104 / 0.069 / — / 9.6
D11 / 0.131 / 0.109 / 0.074 / 0.070 / 0.054 / 0.053 / 0.049 / 0.065 / 0.057 / 0.060 / —
Table S1.9. Pairwise genetic distances of all GermanLacertaagilis populations with DST values shown in the bottom half and corresponding geographic distances in km in the upper half.
D1 / D2 / D3 / D4 / D5 / D6 / D7 / D8 / D9 / D10 / D11D1 / — / 5.3 / 5.3 / 6.5 / 10.4 / 9.1 / 11.9 / 14.6 / 17.9 / 29.7 / 36.7
D2 / 0.419 / — / 6.2 / 3.2 / 5.3 / 6.1 / 10.6 / 13 / 15.2 / 26.9 / 32.9
D3 / 0.248 / 0.350 / — / 4 / 8.9 / 5.1 / 6.8 / 9.8 / 13.2 / 24.3 / 31.9
D4 / 0.149 / 0.414 / 0.197 / — / 4.9 / 3.1 / 7.5 / 9.6 / 12.1 / 23.9 / 30.5
D5 / 0.295 / 0.449 / 0.301 / — / 5.7 / 10.6 / 1.7 / 12 / 23.9 / 28.6
D6 / 0.283 / 0.429 / 0.257 / 0.244 / 0.330 / — / 5.0 / 6.7 / 9.1 / 20.9 / 27.2
D7 / 0.315 / 0.345 / 0.145 / 0.147 / 0.259 / 0.274 / — / 2.7 / 6.5 / 17.6 / 25.4
D8 / 0.230 / 0.387 / 0.254 / 0.211 / 0.265 / 0.243 / 0.160 / — / 4.2 / 14.9 / 22.8
D9 / 0.289 / 0.408 / 0.218 / 0.242 / 0.217 / 0.343 / 0.244 / 0.257 / — / 11.6 / 18.9
D10 / 0.337 / 0.375 / 0.303 / 0.271 / 0.329 / 0.206 / 0.293 / 0.264 / 0.251 / — / 9.6
D11 / 0.184 / 0.365 / 0.193 / 0.149 / 0.182 / 0.264 / 0.162 / 0.137 / 0.199 / 0.217 / —
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Figure S1.1.Correlation of genetic and geographic distances for Lacertaagilis populations in Germany (upper panel) and Bulgaria (lower panel) using DEST, FST, and GST values.
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References
Gullberg A, Tegelström H, Olsson M (1997) Microsatellites in the sand lizard (Lacerta agilis): description, variation, inheritance, and applicability. Biochem. Genetics 35:281–295
Schwartz TS, Olsson M (2008) Microsatellite markers developed for a Swedish population of sand lizard (Lacerta agilis). Conserv Genetics 9:715–717
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