Suppl. 1.Phylogenetic affiliation of the cultured isolates from the sponge G. carnosa.

OTU / Representative
isolates
(accession no) / No. of sequenced isolates in OTU / Closest matches in GenBank
Affiliation division / Strain / IDa
(%) / RAb
(%)
1 / LS36 (FJ937894) / 4 / α-Proteobacteria / Rhodobacterales bacterium (AB294975.1) / 97 / 3.7
2 / LS53 (FJ937900) / 3 / Rhodobacteraceae bacterium (AB180391.1) / 99
3 / LS80 (FJ937909) / 1 / Rhodobacterales bacterium (AB294975.1) / 98
4 / LS96 (FJ937912) / 1 / α-proteobacterium (DQ446159.2) / 98
5 / LS103 (FJ937914) / 1 / α-proteobacterium (DQ446157.2) / 97
6 / LS2 ( FJ937873) / 3 / γ-Proteobacteria / Vibrio parahaemolyticus (AF388387.1) / 99 / 26.0
7 / LS8 (FJ937878) / 3 / Vibrio harveyi (AY911396.1) / 99
8 / LS9 (FJ937879) / 2 / Alteromonas macleodii (AM885870.1) / 99
9 / LS16 (FJ937881) / 2 / Vibrio sinaloensis (DQ451210.1) / 97
10 / LS21 (FJ937884) / 1 / Vibrio sp. (DQ530291.1) / 99
11 / LS25 (FJ937888) / 4 / Photobacterium damselae (AY147861.1) / 99
12 / LS37 ( FJ937895) / 2 / Vibrio parahaemolyticus (EU077545.1) / 99
13 / LS45 (FJ937898) / 1 / Alcanivorax sp. (AB053130.1) / 99
14 / LS76 (FJ937908) / 3 / Vibrio carchiariae (X74693.1) / 99
15 / LS92 (FJ937911) / 1 / Shigella dysenteriae (EU009184.1） / 99
16 / LS133 (FJ937917) / 2 / Marinobacter aquaeolei (AJ000726.1) / 99
17 / LS153 (FJ937919) / 1 / Marinomonas pontica (AY539835.1) / 96
18 / LS169 (FJ937921) / 1 / Uncultured organism (DQ396193.1) / 99
19 / LS172 (FJ937922) / 1 / Pseudomonas poae (AJ492829.1) / 99
20 / LS197 (FJ937924) / 1 / Pseudomonas sp. (AM410618.1 ) / 94
21 / LS227 (FJ937929) / 1 / Pseudomonas poae (EU350370.1) / 97
22 / LS251 (FJ937930) / 1 / γ-proteobacterium (AY576751.1) / 98
23 / LS134 (FJ937918) / 3 / High G+C Gram-positive bacteria / Rhodococcus fascians (AY771765.1) / 99 / 7.3
24 / LS275 (FJ937934) / 4 / Micromonospora floridensis (EU274366.1) / 99
25 / LS279 (FJ937937) / 3 / Micromonospora matsumotoense (AF152109.1) / 98
26 / LS284 (FJ937939) / 1 / Verrucosispora gifhornensis (Y15523.1) / 100
27 / LS286 (FJ937940) / 1 / Gordonia terrae (AB355992.1) / 99
28 / LS287 (FJ937941) / 2 / Micromonospora carbonacea (EU274365.1) / 99
29 / LS288 (FJ937942) / 1 / Pseudonocardia petroleophila (AJ252828.1) / 98
30 / LS296 (FJ937944) / 1 / Sphaerisporanqium rubeum (AB208718.1) / 99
31 / LS298 (FJ937945) / 1 / Streptomyces sp. (DQ448729.1) / 99
32 / LS299 (FJ937946) / 1 / Saccharomonospora sp. (EU214951.1) / 99
33 / LS1 (FJ937872) / 4 / Low G+C Gram-positive bacteria / Bacillus sp. (DQ448757.1) / 99 / 63.0
34 / LS3 (FJ937874) / 3 / Bacillus megaterium (DQ660362.1) / 99
35 / LS4 (FJ937875) / 2 / Bacillus cereus (AY138275.1) / 99
36 / LS5 (FJ937876) / 4 / Halobacillus trueperi (AY505522.1) / 99
37 / LS7 (FJ937877) / 1 / Bacillus sp. (AY228462.1) / 99
38 / LS18 (FJ937882) / 4 / Bacillus sp. (EU107757.1) / 99
39 / LS22 (FJ937885) / 1 / Oceanobacillus oncorhynchi (DQ089679.1) / 99
40 / LS23 (FJ937886) / 5 / Bacterium (AY745842.1) / 99
41 / LS26 (FJ937889) / 1 / Bacillus horikoshii (DQ333286.1) / 98
42 / LS27 (FJ937890) / 4 / Halobacillus trueperi (AY310149.1) / 98
43 / LS29 (FJ937891) / 4 / Bacillus sp. (DQ275185.1) / 98
44 / LS30 (FJ937892) / 15 / Bacillus pumilus (AB048252.1) / 99
45 / LS31 (FJ937893) / 2 / Halobacillus salinus (AF500003.1) / 99
46 / LS42 (FJ937897) / 2 / Bacillus sp. (DQ448746.1) / 99
47 / LS48 (FJ937899) / 1 / Bacillus baekryungensis (AY505501.1) / 96
48 / LS52 (FJ937901) / 1 / Bacillus pumilus (AB048252.1) / 97
49 / LS54 (FJ937902) / 1 / Bacillus flexus (DQ514312.1) / 99
50 / LS56 (FJ937903) / 1 / Bacillus sp. (AB043854.1) / 99
51 / LS59 (FJ937904) / 4 / Pontibacillus chungwhensis (AY603362.1) / 99
52 / LS61 (FJ937906) / 1 / Bacillus sp. (AB062678.1) / 97
53 / LS93 (FJ937910) / 1 / Halobacillus litoralis（X94558.1） / 98
54 / LS97 (FJ937913) / 1 / Brevibacillus agri (AB039334.1) / 99
55 / LS113 (FJ937915) / 1 / Pontibacillus sp. (DQ448765.1) / 99
56 / LS115 (FJ937916) / 1 / Bacillus sp. (DQ448790.1) / 99
57 / LS155 (FJ937920) / 1 / Bacillus gibsonii (AY737309.1) / 99
58 / LS207 (FJ937926) / 1 / Bacillus sp. (DQ453811.1) / 97
59 / LS216 (FJ937927) / 1 / Bacillus flexus (AB021185.1) / 97
60 / LS218 (FJ937928) / 1 / Bacillus baekryungensis (AY505501.1) / 99
61 / LS255 (FJ937931) / 1 / Staphylococcus kloosii (DQ093351.1) / 92
62 / LS262 (FJ937932) / 1 / Bacillus flexus (EU221413.1) / 99

a. “ID” indicates the sequence identity between the isolate and it’s closest relative.

b. “RA” indicates the relative abundance of the affiliation division in the 300 isolates.

Suppl. 2. Phylogenetic affiliation of the 16S rRNA gene clones associated with G. carnosa.

OTU / Representative
Clonesa
(accession no) / No. of sequenced strains in OTU / Closest matches in GenBank
Affiliation division / Strain / IDb
(%) / RAc
(%)
1 / MWLSB154 (FJ937854) / 2 / β-Proteobacteria / Uncultured bacterium (EU236297.1) / 90 / 8.4
2 / MWLSB1 (FJ937832) / 4 / γ-Proteobacteria / Uncultured γ-proteobacterium (EF076171.1) / 96 / 20.1
3 / MWLSB14 (FJ937833) / 3 / Uncultured γ-proteobacterium (EU350859.1) / 94
4 / MWLSB21 (FJ937840) / 3 / Unculturedγ-proteobacterium (EU350860.1) / 98
5 / MWLSB49 (FJ937836) / 2 / Uncultured γ-proteobacterium (EF076171.1) / 95
6 / MWLSB237 (FJ937834) / 1 / Uncultured γ-proteobacterium (EF076171.1) / 89
7 / MWLSB303 (FJ937841) / 1 / Unculturedγ-proteobacterium (EU350859.1) / 98
8 / MWLSA92 (FJ937870) / 10 / High G+C Gram-positive bacteria / Uncultured actinobacterium (EF471579.1) / 99 / 20.9
9 / MWLSB9 (FJ937869) / 3 / Bacterium (AB021325.1) / 93
10 / MWLSB195 (FJ937868) / 1 / Uncultured actinobacterium (EF471577.1) / 91
11 / MWLSA50 (FJ937864) / 1 / Cyanobacteria / Synechococcus sp. (AY881622.1) / 98 / 50.6
12 / MWLSA52 (FJ937865) / 1 / Synechococcus sp. (CT978603.1) / 99
13 / MWLSA69 (FJ937865) / 1 / Synechococcus sp. (AY172826.1) / 98
14 / MWLSA94 (FJ937871) / 1 / Synechococcus sp. (AF001480.1) / 99
15 / MWLSB8 (FJ937842) / 5 / Uncultured bacterium (EF574644.1) / 99
16 / MWLSB12 (FJ937843) / 6 / Uncultured bacterium (EF573670.1) / 93
17 / MWLSB17 (FJ937844) / 6 / Uncultured bacterium (EF574449.1) / 99
18 / MWLSB18 (FJ937860) / 3 / Uncultured bacterium (EF574449.1) / 98
19 / MWLSB24 (FJ937845) / 3 / Uncultured bacterium (EF574057.1) / 93
20 / MWLSB26 (FJ937846) / 8 / Uncultured marine bacterium (EU010210.1) / 99
21 / MWLSB28 (FJ937849) / 3 / Uncultured bacterium (EF575309.1) / 99
22 / MWLSB31 (FJ937850) / 1 / Uncultured marine bacterium (EU010221.1) / 96
23 / MWLSB36 (FJ937851) / 1 / Uncultured bacterium (EF574449.1) / 98
24 / MWLSB53 (FJ937852) / 1 / Uncultured marine bacterium (EU010223.1) / 99
25 / MWLSB101 (FJ937847) / 1 / Uncultured bacterium (EF574449.1) / 94
26 / MWLSB103 (FJ937848) / 1 / Uncultured bacterium (EF574871.1) / 99
27 / MWLSB157 (FJ937855) / 2 / Uncultured marine bacterium (EU010223.1) / 94
28 / MWLSB245 (FJ937857) / 4 / Synechococcus sp. (AY881622.1) / 99
29 / MWLSB264 (FJ937858) / 1 / Synechococcus sp. (AJ639899.1) / 99
30 / MWLSC91 (FJ937861) / 1 / Synechococcus sp. (AY172831.1) / 99
31 / MWLSC92 (FJ937862) / 1 / Synechococcus sp. (AY172803.1) / 99
32 / MWLSC95 (FJ937853) / 1 / Synechococcus sp. (FJ497719.1) / 94
33 / MWLSC102 (FJ937863) / 4 / Uncultured Synechococcus sp. (DQ903987.1) / 99

a. The 16S rRNA gene of the 33 representative clones were distantly related between each other (sequence identity ≤97%). The clones named with “MWLSA--”, “MWLSB--”, and “MWLSC--” were amplified by the primer pairs Ab333F/U1406R, E8F/U1406R, and CYA106F/CYA781R (a+b), respectively.

b. “ID” indicates the sequence identity between the clone and it’s closest relative.

c. “RA” indicates the relative abundance of the affiliation division in the 310 clones.