Supplementary Material

BSND is a novel immunohistochemical marker for oncocytic salivary gland tumors

Shinmura K et al.

Submitted to Pathology & Oncology Research

Supplementary Table S1. Tumor types from the TCGA database analyzed in this study

Supplementary Table S2. Ninety-fifth percentile of mRNA BSND expression values in various tumors using data from the TCGA database

Supplementary Figure S1. Immunohistochemical results for BSND in Warthin’s tumor with squamous metaplasia

Supplementary Table S1. Tumor typesfrom the TCGA database analyzed in this study

Tumor types / TCGA ID / Non-tumorous tissue / Tumorous tissue
Adrenocortical carcinoma / ACC / Not used / Used
Bladder urothelialcarcinoma / BLCA / Used / Used
Breast invasive carcinoma / BRCA / Used / Used
Cervical squamous cell carcinoma and endocervicaladenocarcinoma / CESC / Used / Used
Cholangiocarcinoma / CHOL / Used / Used
Colorectal adenocarcinoma / COAD/ READ / Used / Used
Diffuse large B-cell lymphoma / DLBC / Not used / Used
Esophageal carcinoma / ESCA / Used / Used
Glioblastomamultiforme / GBM / Not used / Used
Head and neck squamous cell carcinoma / HNSC / Used / Used
Chromophobe renal cell carcinoma / KICH / Used / Used
Clear cell renal cell carcinoma / KIRC / Used / Used
Papillary renal cell carcinoma / KIRP / Used / Used
Acute myeloid leukemia / LAML / Not used / Used
Liver hepatocellular carcinoma / LIHC / Used / Used
Brain lower grade glioma / LGG / Not used / Used
Lung adenocarcinoma / LUAD / Used / Used
Lung squamous cell carcinoma / LUSC / Used / Used
Mesothelioma / MESO / Not used / Used
Ovarian serous cystadenocarcinoma / OV / Not used / Used
Pancreatic adenocarcinoma / PAAD / Used / Used
Prostate adenocarcinoma / PRAD / Used / Used
Sarcoma / SARC / Not used / Used
Skin cutaneous melanoma / SKCM / Not used / Used
Stomach adenocarcinoma / STAD / Used / Used
Testicular germ cell tumors / TGCT / Not used / Used
Thyroid carcinoma / THCA / Used / Used
Thymoma / THYM / Not used / Used
Uterine carcinosarcoma / UCS / Not used / Used
Uterine corpus endometrial carcinoma / UCEC / Used / Used
Uvealmelanoma / UVM / Not used / Used

Supplementary Table S2. Ninety-fifth percentile of mRNA BSND expression values in various tumorsusing data from the TCGA database

Tumor / TCGA ID / No. of cases / 95th percentile expression valuea
Adrenocortical carcinoma / ACC / 79 / 0.005437477
Bladder urothelialcarcinoma / BLCA / 408 / 0.013741907
Breast invasive carcinoma / BRCA / 1100 / 0.016930811
Cervical squamous cell carcinoma and endocervicaladenocarcinoma / CESC / 306 / 0.025359907
Cholangiocarcinoma / CHOL / 36 / 0.011975723
Colorectal adenocarcinoma / COAD/ READ / 382 / 0.003765075
Diffuse large B-cell lymphoma / DLBC / 28 / 0.001869111
Esophageal carcinoma / ESCA / 185 / 0.009120745
Glioblastomamultiforme / GBM / 166 / 0.006817318
Head and neck squamous cell carcinoma / HNSC / 522 / 0.022014922
Chromophobe renal cell carcinoma / KICH / 66 / 14.70931956
Clear cell renal cell carcinoma / KIRC / 534 / 0.114419416
Papillary renal cell carcinoma / KIRP / 291 / 0.022616547
Acute myeloid leukemia / LAML / 173 / 0.007432539
Liver hepatocellular carcinoma / LIHC / 373 / 0.058516993
Brain lower grade glioma / LGG / 530 / 0.002568545
Lung adenocarcinoma / LUAD / 517 / 0.022675142
Lung squamous cell carcinoma / LUSC / 501 / 0.028700827
Mesothelioma / MESO / 86 / 0.00999883
Ovarian serous cystadenocarcinoma / OV / 307 / 0.102314666
Pancreatic adenocarcinoma / PAAD / 179 / 0.014219691
Prostate adenocarcinoma / PRAD / 498 / 0.017994325
Sarcoma / SARC / 262 / 0.0041288
Skin cutaneous melanoma / SKCM / 471 / 0.002427157
Stomach adenocarcinoma / STAD / 36 / 0.007060476
Testicular germ cell tumors / TGCT / 156 / 0.159522159
Thyroid carcinoma / THCA / 509 / 0.001911383
Thymoma / THYM / 120 / 0.041183724
Uterine carcinosarcoma / UCS / 57 / 0.02392328
Uterine corpus endometrial carcinoma / UCEC / 177 / 0.046346454
Uvealmelanoma / UVM / 80 / 0

aThe RSEM expression value of the BSND gene was divided by that of the TBP gene.

Supplementary Figure S1. Immunohistochemical results for BSND in Warthin’s tumor with squamous metaplasia. Immunohistochemistry revealed that percentage of the cells showing BSND positivity was reducedin squamous metaplastic lesionsofWarthin’s tumor (A, B)compared with the other epithelial lesions of the Warthin’s tumor(C, D). Hematoxylin and eosin stain: (A) and (C). Scale bar = 20 m.