Stubborn disease in Iran: Diversity of Spiroplasmacitri strains in Circuliferhaematoceps leafhoppers collected in sesame fields in Fars province.
Supplementary Information
Figure S1. Map of Fars province showing sites, at which Spiroplasma citri was detected in the present () and last study (●) [1].
Figure S2. Agarose gel electrophoresis of PCR products (817 bp) amplified from symptomatic periwinkle plants using primer pairs F1/R1.
Table S1.Spiroplasmaspecies and strains of S.citri used in molecular analyses.
Table S2.Homology matrix of spiralin sequences.
Figure S1. Map of Fars province showing sites, at which Spiroplasma citri was detected in the present () and last study (●) [1].
Figure S2. Agarose gel electrophoresis of PCR products (817 bp) amplified from symptomatic periwinkle plants using primer pairs F1/R1. Lane 1, negative control; Lanes 2-9 periwinkle plants exposed to C. haematocepscollected in sesame fields in of Fars province (Iran) including Abade I, Marvdasht I, Juyom I, Firuzabad I, Firuzabad II, Zarghan I, GhirKarzin I, GhirKarzin II; Lane M, 100 bp DNA ladder.
Table S1:Spiroplasmaspecies and strains of S.citri used in molecular analyses.
Acc. No. / Host / Geographical / Strains/ Species / No.KP148811 / Leafhopper / AbadeTashk, Iran / AbadeTashk / 1
U13994 / Citrus sinensis / Not available / Alcanar 254 / 2
U13995 / Leafhopper / France / Corsica / 3
KP666137 / Leafhopper / Darab, Iran / Darab II / 4
KP666138 / Leafhopper / Darab, Iran / Darab IV / 5
KP666139 / Sesame / Darab, Iran / Darab XVIII / 6
FJ755921 / Leafhoppers / Fasa, Iran / Fasa I / 7
JN974242 / Sweet orange / Fasa, Iran / Fasa II / 8
FJ755922 / Leafhoppers / Fasa, Iran / Fasa III / 9
AM157770 / Citrus sinensis / Egypt / Fewa / 10
KP148817 / Leafhopper / Firuzabad, Iran / Firuzabad I / 11
KP148818 / Leafhopper / Firuzabad, Iran / Firuzabad II / 12
KP148815 / Leafhopper / GhirKarzin, Iran / GhirKarzin I / 13
KP148816 / Leafhopper / GhirKarzin, Iran / GhirKarzin II / 14
AM285305 / Leafhopper / Morocco / GII-3 / 15
U13996 / Citrus sinensis / Israel / Israel/asp1 / 16
KP148813 / Leafhopper / Juyom, Iran / Juyom I / 17
JN974243 / Sesame / Khafr, Iran / Khafr I / 18
KP148812 / Leafhopper / Marvdasht, Iran / Marvdasht I / 19
U13997 / Leafhopper / Syria / Palmyra / 20
U13993 / Citrus leaves / Morocco / R8A2B / 21
JN974239
JN974240
JN974241 / Safflower
Safflower
Safflower / Shiraz, Iran
Shiraz, Iran
Shiraz, Iran / Saf I
Saf II
Saf III / 22
23
24
JN860712 / Local sweet orange / Shiraz, Iran / Shiraz / 25
U57659 / Corn / Alajuela, Costa Rica / S.kunkelii/CR2 / 26
M59366 / Honey bee / Maryland / S.melliferum/TS-2 / 27
U57658 / Periwinkle / Middle East / S.phoeniceum / 28
KP148814 / Leafhopper\ / Zarghan, Iran / Zarghan I / 29
U13993 / Citrus sinensis / Not available / 78 / 30
Table S2: Homology matrix of spiralin sequences.
13 / 12 / 11 / 10 / 9 / 8 / 7 / 6 / 5 / 4 / 3 / 2 / 1 / Strains/Isolates٭100% / 1-Abade I
100% / 100.0% / 2-Marvdasht I
100% / 94.0% / 94.0% / 3-Ghirkarzin I
100% / 100.0% / 94.0% / 94.0% / 4-Ghirkarzin II
100% / 100.0% / 100.0% / 94.0% / 94.0% / 5-Juyom I
100% / 99.9% / 99.9% / 99.9% / 93.9% / 93.9% / 6-Zarghan I
100% / 99.7% / 99.9% / 99.9% / 99.9% / 93.9% / 93.9% / 7-Firuzabad I
100% / 96.9% / 96.6% / 96.7% / 96.7% / 96.7% / 90.9% / 90.9% / 8-Firuzabad II
100% / 96.7% / 99.9% / 99.9% / 100.0% / 100.0% / 100.0% / 94.0% / 94.0% / 9-GII-3
100% / 98.6% / 95.4% / 98.5% / 98.5% / 98.6% / 98.6% / 98.6% / 94.4% / 94.4% / 10-Corse
100% / 99.5% / 98.1% / 94.8% / 98.0% / 98.0% / 98.1% / 98.1% / 98.1% / 93.9% / 93.9% / 11-R8A2B
100% / 95.8% / 96.3% / 95.4% / 92.3% / 95.3% / 95.3% / 95.4% / 95.4% / 95.4% / 97.8% / 97.8% / 12-Palmyre
100% / 88.0% / 89.8% / 89.9% / 89.0% / 86.0% / 88.8% / 88.8% / 89.0% / 89.0% / 89.0% / 88.0% / 88.0% / 13-S. melliferum
٭ See Table1 of the main manuscript for abbreviations and accession numbers
Reference
- Khanchezar A, Béven L, Izadpanah K, Salehi M, Saillard C (2014) Spiralin diversity within Iranian strains of Spiroplasma citri. Curr Microbiol 68: 96-104.