PATCH RELEASE NOTES

Sequencing Analysis Software version 6.2

Updated Aug10, 2017

CONTENTS

OVERVIEW

CHANGES IN SEQUENCING ANALYSIS SOFTWARE VERSION 6.2

SYSTEM REQUIREMENTS

PATCH INSTALLATION INSTRUCTIONS

OVERVIEW

Sequencing Analysis Software version 6.2 is a patch release intended to update current Sequencing Analysis Software 6version6.xinstallations to support Applied Biosystems™ SeqStudio™ Genetic Analyzer. You can also download the patch release from Thermo Fisher Scientific website.

CHANGES IN SEQUENCING ANALYSIS SOFTWARE VERSION 6.2

  • Sequencing Analysis Software v6.2 supports Applied Biosystems SEQSTUDIO™ Genetic Analyzer.
  • Sequencing AnalysisSoftware v6.2 uses KB Basecaller version 1.4.2.4 for basecalling.

Below are the improvements in KB Basecaller version 1.4.2.4over version 1.4.1.8

  • Sequencing Analysis Software 6 crashes when basecalling some .ab1 files when the begin position of first scan peak is not valid.This issue was fixed in this version.
  • Autoanalysis of UDC 4.0 also crashes due to the basecalling some .ab1 files when the begin position of first scan peak is not valid.This issue was fixed in this version.
  • Some .ab1 files with very low quality and secondary sequence throughout causing basecall analysis failure.This issue was fixed in this version.
  • Update to DB1L tag in the 3730 BigDye Direct calibration file (KB_3730_POP7_BDTv3direct.bcc) to fix the issues of reporting wrong start point when BDD mobility file is used.
  • New calibration files added to support new configurations, specifically, new run modules, new chemistry (BigDyeDirect), new array length (3500 36-cm array), and POP4 on 3500instrument.
  • Eliminate the effect of saturated scans on the estimation of correction matrix.

SYSTEM REQUIREMENTS

This patch can only be applied on Sequencing Analysis Software version 6.x.

PATCH INSTALLATION INSTRUCTIONS

In order to install this patch release, do the following:

  1. LocateSequencing Analysis Software v6.2patch installation files.
  2. Close the Sequencing Analysis Softwarev6.x software.
  3. Unzip the downloaded patch.
  4. Double click the file named "SeqA6.2_Patch_Installer.bat" to launch the patch installer.
  5. Follow further instructions provided by the installer.
  6. After installation is complete, restart the computer.

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