/ Royal Society of Chemistry Postgraduate Student Conference in Food Science
Queen’s University Belfast
7-9 July 2008

See example on next page.

Please provide us with details below exactly as you wish them to be presented on the conference website and in the conference programme.

Title of Presentation: ______

Abstract of presentation (no more than 300 words including title, authors names and affiliation):

Please indicate your preference:

Poster Oral Presentation No Preference 

NB. All students must make either an oral or poster presentation.

Privacy Act: This information will be used by the Conference Organising Committee for the purposes of their conference. A list of delegates will also be made available to other delegates and exhibitors and sponsors of the conference. Please tick here if you DO NOT wish to have your name and particulars included on any such lists 

It is suggested that those presenting posters prepare a handout (A4 black & white photocopies) to be left with the poster for those interested in your research.

Means to display posters will be provided on arrival.

EXAMPLE

Title of Presentation: _LC-MS analysis of AGEsin foods and related systems ______

Abstract of presentation (no more than 300 words in Ariel 11):

LC-MS analysis of AGEsin foods and related systems
Shima Assar, Permal Deo, Catherine Moloney, Maria Lima and Jennifer M. Ames
Institute of Agri-Food and Land Use, School of Biological Sciences, David Keir Building, Stranmillis Road, Queen’s University Belfast, BT9 5AG, Northern Ireland.
Various amino acid residues within protein undergo modification on exposure to carbohydrate, lipid or reactive oxygen species to form advanced glycation endproducts (AGEs), advanced lipoxidation endproducts (ALEs) and other markers of oxidative stress. The AGE/ALE, Nε-(carboxymethyl)lysine (CML) has been identified in various foods, including dairy products and bakery items and whole meals. The aim of this study was to assess the suitability of different procedures for the isolation of protein from foods and subsequent hydrolysis to amino acids prior to analysis by ultrapressure liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).
Foods selected for analysis included bovine milks, infant formulae, and breadcrust. Protein was isolated by the Folch procedure, precipitation with trichloroacetic acid or ultrafiltration prior to hydrolysis with 6M HCl. Following clean-up on a C18 cartridge, hydrolysates were analysed by UPLC-MS/MS.
Nonafluoropentanoic acid (NFPA) as the mobile phase modifier gave better resolution of compounds and better MS signal compared to trifluoroacetic acid. Good data for CML could be achieved with as little as 2 μg protein-equivalent on-column.
In conclusion, different foods required different sample work-up due to differences in composition, physical form and degree of heat processing. Application of appropriate procedures resulted in the UPLC-MS/MS analysis of CML in a range of commodities.

Please indicate your preference:

Poster xOral Presentation No Preference 

Further Information:

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