Section B: Breeding Colony
Complete this form when breeding mice and rats to maintain experimental colonies. Submit completed forms to . To comply with NIH OBA guidelines, any transgenic animal strain that is crossed to a wild type strain or a different transgenic strain requires notification of the Institutional Biosafety Committee (IBC) before the breeding is initiated. Please contact Mariel Jais at ith any questions about IBC review.
Date: []
Investigator: []
Contact Person (if other than PI): []
IACUC Protocol Number(s): []
Animal Holding Room Location: []
Contact Person and Phone Number for after hours/ weekend emergencies: []
Species: Mice Rats Other: []
Strain(s):
- []
- []
- []
- []
- []
Source:
Jackson Labs Taconic Harlan NCI Charles River Hilltop
Other: []
Catalog/strain identification number: []
If breeders are obtained from a source other than one of the approved commercial vendors listed above, a health report must be reviewed and approved by the Attending Veterinarian before animals will be allowed into the facility. Contact Dr. Patricia Foley at foleyp@.gwu.edu with any questions.
Breeding Scheme:(Please describe in detail your breeding scheme – note: if harem breeding, pregnant females MUST be removed to separate cages BEFORE they deliver)
Homozygous Heterozygous
How many breeders are paired in one cage?
Males: []Females:[]
Are the females removed to separate cages to deliver and wean litters?
Yes No
At what age are pups weaned? []
What is the expected average litter size?
What percentage of pups is expected to survive to weaning? []
At what age are breeders initially mated?
Males: []Females: []
At what point are breeders retired, i.e., age, number of litters, etc.? []
What method of identification is used for individual animals?
Ear tag Ear punch Tattoo None Other: []
For transgenic strains, what testing is done to determine genotype, and at what age are the animals tested? []
What percentage of pups is expected to be unusable for experiments due to incorrect genotype, age, gender, or other factors? []
Describe the final disposition of any unusable animals, i.e., transfer to another PI, euthanasia, etc. []
What additional strategies are required for poorly breeding strains: (e.g. light cycle, enrichment, ensuring that animals from early litters are used as dams/sires, etc.) []
Quality control: For KO and transgenic strains what testing is done to ensure correct genotype is maintained?
Copy of breeding card: (contact ARF (202-994-2871) – Marissa or Rob with help creating card, if needed).
Note that information on breeding cards should include:
- Investigator Name
- IACUC protocol number
- Cage #
- Dam and Sire identification & strain
- Date breeding pair established
- # of pups born and date of birth for each litter
- # of pups weaned of each sex and date of weaning
Updated 9/2012 - AMM