Copy number variation of chromosome 5A and its association with Q gene expression, morphological aberrations, and agronomic performance of winter wheat cultivars.

Sebastian Förster* · Erika Schumann · Mario Baumann · W. Eberhard Weber · Klaus Pillen

Institute of Agricultural and Nutritional Sciences, Martin-Luther-University Halle-Wittenberg, Betty-Heimann-Str. 3, 06120 Halle (Saale), Germany

*

Supplementary Fig 2. PCR amplification of microsatellite Xbarc56 (http://wheat.pw.usda.gov/ggpages/SSRclub/GeneticPhysical/) exemplarily shown for 23 randomly selected progeny plants of cultivar ‘Batis’.

A single plant (column 4), having no PCR product, corresponds to the fully awned speltoid plant mentioned in Table 1.

Supplementary Fig. 4 Amplification plots (a-d) and melting curves (e-f) of actin and the 5AQ gene from young spikes of the CS mutant fndel-143 and disomic CS in three dilution steps.

a and c show amplification plots of the actin gene from the two lines in three dilution steps (dilution factor 4). b and d show amplification plots of the 5AQ gene from the two lines, again in three dilution steps (dilution factor 4). e and f show melting curves of actin and the 5AQ gene from the two lines for the three dilutions.

Supplementary Table 1 Pearson correlation (r) between and within 18 agronomic traits and relative 5AQ gene expression in normal and speltoid plants

Trait / SPL / SPN / SPD / GEA / FER / WEI / THR / LFL / WFL / PLE / TIL / HEA / FLT / TKW / GAR / GAW / GAL / HEI / EXP 5AQ / Trait
SPL / 0.59 / 0.60 / -0.28 / 0.65 / 0.33 / 0.52 / -0.49 / 0.64 / 0.66 / 0.14 / -0.04 / 0.31 / 0.49 / -0.31 / -0.19 / -0.30 / -0.02 / 0.66 / 0.03 / Spike length in cm
SPN / 0.55 / 0.83 / 0.60 / 0.18 / -0.30 / 0.48 / -0.36 / 0.33 / 0.40 / 0.67 / -0.41 / -0.07 / 0.04 / 0.20 / 0.23 / 0.25 / 0.14 / 0.75 / 0.05 / Number of spikelets
SPD / -0.30 / 0.63 / 0.74 / -0.45 / -0.71 / 0.02 / 0.05 / -0.25 / -0.18 / 0.65 / -0.45 / -0.36 / -0.42 / 0.54 / 0.46 / 0.60 / 0.19 / 0.26 / 0.00 / Spike density
GEA / -0.05 / 0.01 / 0.06 / 0.43 / 0.88 / 0.42 / 0.06 / 0.40 / 0.35 / -0.09 / 0.30 / 0.14 / 0.37 / -0.69 / -0.70 / -0.75 / -0.54 / 0.09 / 0.39 / Grain number
FER / -0.43 / -0.74 / -0.44 / 0.66 / 0.61 / 0.23 / 0.25 / 0.23 / 0.16 / -0.37 / 0.45 / 0.11 / 0.28 / -0.73 / -0.76 / -0.83 / -0.58 / -0.30 / 0.39 / Fertility
WEI / 0.52 / 0.40 / -0.03 / 0.05 / -0.24 / 0.49 / -0.27 / 0.23 / 0.54 / 0.55 / -0.28 / -0.43 / -0.29 / 0.34 / 0.27 / 0.22 / 0.21 / 0.28 / 0.44 / Grain weight in g
THR / -0.05 / 0.32 / 0.43 / 0.12 / -0.15 / 0.28 / 0.37 / -0.14 / -0.30 / -0.05 / 0.06 / -0.56 / -0.50 / -0.21 / -0.48 / -0.32 / -0.60 / -0.35 / 0.07 / Threshability in %
LFL / -0.06 / 0.35 / 0.44 / 0.53 / 0.09 / -0.09 / -0.15 / 0.44 / 0.60 / 0.24 / -0.11 / 0.06 / 0.10 / -0.34 / -0.24 / -0.39 / -0.04 / 0.20 / -0.07 / Flag leaf length in cm
WFL / 0.58 / 0.39 / -0.09 / 0.30 / -0.08 / 0.47 / -0.39 / 0.41 / 0.80 / 0.50 / -0.57 / -0.16 / -0.06 / -0.06 / 0.03 / -0.11 / 0.09 / 0.46 / 0.50 / Flag leaf width in cm
PLE / 0.28 / 0.48 / 0.29 / 0.49 / -0.04 / 0.15 / 0.45 / 0.33 / -0.01 / 0.40 / -0.64 / -0.66 / -0.61 / 0.50 / 0.36 / 0.47 / 0.11 / 0.40 / 0.34 / Peduncle length in cm
TIL / -0.19 / -0.62 / -0.55 / -0.44 / 0.15 / -0.33 / -0.41 / -0.21 / -0.38 / -0.46 / 0.69 / 0.43 / 0.52 / -0.45 / -0.54 / -0.39 / -0.49 / -0.33 / -0.45 / Number of tillers
HEA / -0.20 / -0.38 / -0.25 / -0.33 / 0.03 / -0.70 / -0.64 / -0.02 / -0.13 / -0.45 / 0.67 / 0.87 / 0.95 / -0.51 / -0.19 / -0.35 / 0.10 / 0.06 / -0.32 / Ear emergence in days after 1st May
FLT / 0.00 / -0.23 / -0.26 / -0.24 / -0.02 / -0.68 / -0.67 / 0.08 / 0.04 / -0.34 / 0.54 / 0.96 / 0.91 / -0.61 / -0.38 / -0.46 / -0.13 / 0.22 / -0.27 / Flowering in days after 1st May
TKW / 0.47 / 0.33 / -0.07 / -0.52 / -0.57 / 0.83 / 0.17 / -0.39 / 0.22 / -0.13 / -0.01 / -0.39 / -0.43 / 0.91 / 0.91 / 0.97 / 0.65 / 0.14 / -0.10 / Thousand kernel weight in g
GAR / 0.52 / 0.32 / -0.13 / -0.50 / -0.55 / 0.80 / -0.03 / -0.35 / 0.35 / -0.24 / 0.03 / -0.26 / -0.28 / 0.97 / 0.94 / 0.90 / 0.90 / 0.14 / -0.07 / Grain area in mm2
GAW / 0.47 / 0.29 / -0.12 / -0.52 / -0.55 / 0.76 / 0.11 / -0.34 / 0.22 / -0.04 / 0.10 / -0.25 / -0.28 / 0.96 / 0.92 / 0.94 / 0.64 / 0.22 / -0.18 / Grain width in mm
GAL / 0.49 / 0.25 / -0.17 / -0.34 / -0.40 / 0.70 / -0.22 / -0.27 / 0.41 / -0.37 / 0.06 / -0.13 / -0.15 / 0.80 / 0.91 / 0.70 / 0.93 / 0.04 / -0.09 / Grain length in mm
HEI / 0.62 / 0.59 / 0.10 / 0.13 / -0.35 / 0.26 / 0.13 / 0.27 / 0.52 / 0.46 / -0.25 / 0.02 / 0.19 / 0.17 / 0.16 / 0.34 / 0.03 / 0.99 / -0.18 / Plant height in cm
EXP 5AQ / 0.09 / 0.25 / 0.19 / 0.28 / 0.01 / -0.01 / 0.05 / 0.07 / 0.17 / 0.54 / -0.69 / -0.40 / -0.29 / -0.19 / -0.24 / -0.20 / -0.35 / 0.06 / 0.66 / Rel. expression of the 5AQ gene

The lower and upper triangles show correlation coefficients calculated from means of the ten cultivars from normal and speltoid plants, respectively, for agronomic traits and 5AQ gene expression. Boxed cells along the diagonal show correlation coefficients for each trait between cultivar means of normal and speltoid plants, respectively. Bold correlation coefficients are significant with P = 0.05. Positive and negative correlations are color-coded in green and red, respectively. The strength of the color corresponds to the strength of the correlation.

References

Förster S, Schumann E, Weber WE, Pillen K (2012) Discrimination of alleles and copy numbers at the Q locus in hexaploid wheat using quantitative pyrosequencing. Euphytica 186:207-218

Kosuge K, Watanabe N, Kuboyama T, Melnik VM, Yanchenko VI, Rosova MA, Goncharov NP (2008) Cytological and microsatellite mapping of mutant genes for spherical grain and compact spikes in durum wheat. Euphytica 159:289-296

Schubert AC (2009) Quantitative trait loci analysis in spring wheat comparing two advanced backcross populations derived from an exotic wheat accession. Rheinische Friedrich-Wilhelms-Universität Bonn, Dissertation Appendix 3