Article Title: Genetic Variation in the Green Anole Lizard (Anoliscarolinensis) Reveals Island Refugia and a Fragmented Florida During the Quaternary

Journal Name:

Genetica

Author Names:

Marc Tollis and StéphaneBoissinot

Corresponding author:

Marc Tollis

School of Life Sciences Arizona State University

Email:

Table 1.Information about the Florida collecting localities unique to this study. BL: Boissinot Lab; HL: Hsieh Lab.

Location / Latitude / Longitude / Source / Sample Size
East Titusville / 28.5437777 / -80.9421666 / BL / 9
East Cocoa / 28.243611 / -80.870556 / BL / 8
West Vero Beach / 27.640278 / -80.59475 / BL / 6
South Bay / 26.683333 / -80.716884 / BL / 6
Lake Josephine / 27.366783 / -81.400155 / BL / 2
Lake Alfred / 28.078317 / -81.729533 / BL / 4
Miami / 25.736883 / -80.267133 / HL / 1
Miami / 25.739417 / -80.2654 / HL / 1
Miami / 25.734517 / -80.274183 / HL / 1
Miami / 25.931133 / -80.943883 / HL / 1
Miami / 25.757433 / -80.267967 / HL / 1
Miami / 25.75175 / -80.264317 / HL / 1
Miami / 25.738915 / -80.265445 / HL / 1
Miami / 25.679317 / -80.2734 / HL / 1
Tampa / 28.06165 / -82.4164 / HL / 3
Deer Island / 28.796944 / -81.678333 / HL / 3
Gainesville / 29.651999 / -82.324993 / HL / 1

Table 2.GenBank accession numbers and Florida locations for 25 NADH-2 sequences used in this study.

Accession # / Florida Location
JX524368 / Jacksonville
JX524369 / Jacksonville
JX524370 / Jacksonville
JX524371 / Jacksonville
JX524372 / Jacksonville
JX524373 / Chuluota
JX524374 / Chuluota
JX524375 / Chuluota
JX524376 / Chuluota
JX524377 / Chuluota
JX524408 / Palm Beach
JX524409 / Palm Beach
JX524410 / Palm Beach
JX524411 / Palm Beach
JX524412 / Palm Beach
JX524413 / Naples
JX524414 / Naples
JX524415 / Naples
JX524416 / Naples
JX524417 / Panama City
JX524418 / Panama City
JX524419 / Panama City
JX524420 / Panama City
JX524421 / Panama City
JX524422 / Panama City

Figure 1. Maximum likelihood (ML) tree of the HMGCS nuclear gene for 109 green anoles reconstructed with PHYML. Statistical robustness was assessed with 500 bootstrap replicates and is indicated below relatively well-supported nodes. Colored circle size is proportional to the number of individuals in each clade. Blue: Suwannee. Magenta: Everglades. Brown: Central Florida. Yellow: North Carolina. Green: Gulf-Atlantic. The tree is rooted with A. porcatus.

Figure 2. ML tree of the RALGAPA nuclear gene for 104 green anoles reconstructed with PHYML, assessed with 500 bootstrap replicates. Node labels, colored circle key and outgroup are the same as Supplementary Material Figure 1.

Figure 3. ML tree of the TERT gene for 100 green anoles reconstructed with PHYML and assessed with 500 bootstrap replicates. Node labels, colored circle key and outgroup are the same as Supplemtary Material Figures 1 and 2.

Figure 4.Extended Bayesian Skyline Plot depicting population size changes over time within the Central Florida population of A. carolinensis. The solid line is the mean estimate from the posterior distribution of population size at time intervals. Dotted lines indicates the upper and lower bounds of the 95% HPD (highest posterior density). The population size function in this analysis is Ne/generation time; for A. carolinensis we assume a generation time of 1 year.