Research/ Laboratory Standard Operating Procedure
This is a TEMPLATE ONLY until all applicable appendices and/or UAA Chemical SOPs are attached and the completed document signed by the PI and an EHSRMS&EM designee.
Click here to Enter Procedure or Experiment Title, Chemical name or Class of chemical
#1 Laboratory Information
Department / Click here to enter text. /Principle Investigator(s) / Click here to enter PI name(s); can list more than one for multidisciplinary projects /
Location(s) covered by this SOP / Click here to enter building(s), room(s) /
Creation date / Click here to enter a date. /
SOP Author / Click here to enter text. /
Revision date / Click here to enter a date. /
#2 Type of SOP
☐Specific laboratory procedure or experiment
(Examples: synthesis of chemiluminescent esters, folate functionalization of polymeric micelles)
☐Generic laboratory procedure
(Examples: distillation, RNA extraction)
☐Generic use of specific chemical or class of chemicals with similar hazards
(Examples: Ethidium bromide, mineral acids)
#3 Brief Description of Procedure or Experiment
Click here to enter text. Describe what the purpose of the procedure or experiment. Do not include step-by-step instructions here.
#4 Hazard Assessment - Equipment/ Mechanical / Electrical Hazard
Click here to enter text. Describe all equipment that poses a hazard such as power tools, cutting instruments, needles, scalpel blades, etc.
Hazard Mitigation
Click here to enter text. Describe what engineering controls and PPE are required to minimize exposure to the above hazards.
#5 Hazard Assessment – Biological, Chemical, Radiation
Biological Hazard☐Not applicable
Micro-organisms☐BSL1 ☐BSL2
Body Fluids/ Tissues☐Animal☐Human
Complete a copy ofAppendix B - Biological Material Hazards Assessment form, for each hazard class checked above.
Radiation Hazard☐Not applicable
☐Non-ionizing (microwave, infrared, laser, ultraviolet)
Eye Protection: Click here to enter text. Specify what type of eye protection is required for the above checked hazard.
☐Ionizing - Type: ☐Alpha emitter☐Beta emitter☐Gamma emitter☐X-ray
If any of the ionizing radiation boxes are checked, click on the link below and fill out the UAA Radiation Use Request Form. Contact the Radiation Safety Officer for assistance – 907-786-1268 or r
Chemical Hazard
List all chemicals and reagents used in this SOP. Copy and insert more rows as needed.
Chemical/ Reagent name / CAS# (if none, enter ‘see SDS’) / GHS Non-hazardous?Y or N
Complete a copy of Appendix C -Chemical Hazard Assessment formfor EACH hazardous chemical (GHS non-hazardous listed as N or No) listed above. Where available, UAA Chemical Hazard Assessments may be used in place of the Appendix C.
#6Step-by-Step Operating Procedure
For each step’s description, include any step-specific hazard, PPE, engineering controls and designated work area in the left-hand column (under Step-by-Step Description).
Guidance on Engineering and Ventilation Controls – Review safety literature and peer-reviewed journal articles to determine appropriate engineering and ventilation controls for your process or experiment.
Guidance on Personal Protective Equipment – To ensure gloves are compatible with and will provide protection from the chemicals in your SOP, refer to the EHSRSM&EM website under ‘Laboratory Safety’ or the glove selection chart from the links below:
OR
Click on ‘search’ enter ‘glove chart’ and select the second item
Designated work area(s) – Required whenever carcinogens, highly acutely toxic materials or reproductive toxins are used. The intent of a designated work area is to limit and minimize possible sources of exposure to these materials. The entire laboratory, a portion of the laboratory or a laboratory fume hood or bench may be considered a designated work area. This area MUST be wiped down following EACH use to minimize possible exposure.
Procedure - Step-by-Step Description / Potential Risks if Step is Not Done or Done Incorrectly (if any)- Don personal protective equipment
- Check the location/ accessibility/ certification of safety equipment that serves your lab. Notify the PI/ RLS of any deficiencies.
- Describe the next step in the procedure
- Describe the next step in the procedure. Insert additional rows in table, as needed.
- Dispose of hazardous solvents, solutions, mixtures, and reaction residues as hazardous waste. See Waste Disposal section for more information.
- Clean up work area and lab equipment.
- Remove PPE and wash hands.
#7Emergency Procedures
Health-Threatening Emergencies
Fire, explosion, health-threatening hazardous material spill or release, compressed gas leak, valve failure, etc.
CALL 911
Alert people in the immediate vicinity
Pull Fire Alarm
Evacuate building and go to your Emergency Assembly Area: Click here to enter text. Indicate EAA location(s) for the building(s) listed for this SOP
Remain nearby to advise emergency responders
Once personal safety is established, call EHS
Provide local notifications (see Emergency Phone Numbers)
Note: For compressed gas leaks, shut off gas supply ONLY if this can be done safely, without risk to personnel.
Injuries and Exposures
Remove the injured/ exposed individual from the area, unless it is unsafe to do so because of the
medical condition of the victim or the potential hazard to rescuers.
Call 911 if immediate medical attention is needed.
Call EHS to report the exposure (see Emergency Phone Numbers)
Administer first aid as appropriate.
Flush contamination from eyes/ skin using the nearest emergency eyewash/shower for a minimum of 15 minutes. Remove any contaminated clothing. Fire blankets and sweat suits are available in every laboratory for use as needed.
Bring to the hospital copies of SDSs for all chemicals the victim was exposed to.
Non-Health-Threatening Emergencies
Injuries and Exposures
Flush contamination from eyes/skin for a minimum of 15 minutes.
Administer appropriate first aid.
Go to EHS website to report the incident on Origami.
Small/ manageable spills
In the event of a minor spill or release that can be safely cleaned up using laboratory PPE and spill kit (Amphomag), spills or releases which have been contained within the laboratory (via secondary containment, in a fume hood, on a bench or the floor), spills of less than 500 mL of any flammable material, or spills of materials that do not require respiratory protection for cleanup:
Notify personnel in the area and keep people away from the area. Eliminate all sources of ignition.
Protect yourself:
Review the SDS for the spilled material, or use your knowledge of the hazards of the material to determine the appropriate level of protection.
Wearing appropriate PPE, clean up the spill with Amphomag following the directions provided in the spill kit. Collect spill cleanup materials in a tightly closed container or double plastic bags. Manage spill cleanup debris as hazardous waste.Contact EHS for waste pickup.
Dispose of broken glass in a broken glass container – DO NOT put other materials in the broken glass container.
DO NOT clean up spills requiring respiratory protection – CALL EHS.
Large/ unmanageable spills
For highly reactive, highly acute toxin, spills or releases which have impacted the environment (via sink / sewer system, soil or air outside the building), spills larger than 500mL of any flammable material, or spill of any material requiring respiratory protection for cleanup:
Protect yourself and others:
Contact PI/ RLS and EHS personnel on Emergency Phone Number list.
Alert people in the vicinity of the spill, advise them to evacuate the immediate area.
Restrict access to the area of the spill. Eliminate all sources of ignition on your way out.
Evacuate to a safe area. Remain nearby to advise PI and EHS.
Lab-Specific (Biological/Chemical/Radiation Hazard specific) Procedures
This section is for any emergency procedures different from standard responses, or for additional emergency information due to the nature of materials or task. Include information on gas leaks, chemical spills, and personal exposure / medical emergency as appropriate (e.g. Calgonate gel for hydrofluoric acid exposure).
#8Decontamination/ Waste Disposal and Pollution Prevention
Decontaminate disposable items (e.g. pipet tips, plates) and empty chemical containers by triple rinsing with a liquid that will dissolve the material.
If water is the solvent, the first rinsing only must go into an appropriate waste container, the remaining rinses contain de minimus quantities of hazardous material and may go in the sink with the water running.
If a non-aqueous solvent is used, all rinsesmust go into an appropriate waste container and the rinsed container placed in a fume hood to allow remaining vapors to be drawn up the hood.
Decontaminated items can go in non-hazardous trash.
Identify amounts of waste anticipated and appropriate disposal procedures. Segregate waste by hazard class (flammable, corrosive, etc.), state (liquid, solid) and, for organic solvents halogenated and non-halogenated. Store waste appropriately for the hazard class. Contact the CHO or CAS Stockroom Manager if you need assistance.
General hazardous waste disposal guidelines
Label Waste
Affix hazardous waste label on all waste containers as soon as the first drop of waste is added to the
container.
DO NOT share waste containers with other research groups. Each research group shall maintain their own
waste containers for their procedures.
Store Waste
Store hazardous waste in closed containers, in secondary containment, and in your laboratory’s
designated location. Waste containers MUST be closed at all times, except when waste is being added tothe container.
Waste containers are considered FULL when they contain approximately 75% of the maximum volume.
DO NOT OVERFILL – this is an exposure hazard for all persons handling and disposing of the waste.
Dispose of Waste
Call EHS or Lab Support (CAS only) to have full waste containers picked up for disposal by EHS.
Methods of Disposal ☐Consumed during process
☐Neutralized as part of procedure
☐Sink disposal (GHS non-hazardous liquids only – MUST HAVE EHS approval)
☐Hazardous waste by EHS (will be lab-packed by a Hazardous Waste
Management company if cannot go to a sanitary landfill; associated disposal
costs may be assessed to your research or department budget)
Chemical specific instructions:
Click here to enter text. Describe the measures to take, procedures to follow for proper disposal or packaging of chemical waste.
#9Training Requirements
Prior to conducting any work in this SOP, the PI or designee must provide training to his/her laboratory personnel specific to the hazards involved in working with this substance, work area decontamination and emergency procedures.
The PI must provide or make readily available a copy of this SOP and copies of SDSs provided by the manufacturer.
The PI must ensure that his/her laboratory personnel have attended appropriate and required laboratory safety training or refresher training within the last 12 months.
Lab-Specific Training
Click here to enter text. List any additional training required before laboratory personnel can begin work using this SOP.
Location of SDS(s)
UAA uses a web-based chemical inventory system, CISPro, which can be viewed anywhere an internet connection is available. CISPro can be viewed using Internet Explorer, Mozilla Firefox and Google Chrome. If your browser does not permit access to CISPro, please use one listed.
SDS(s) are available without a password at:
PI’s have a secure login to view their chemical inventory and SDSs on CISPro and may choose to make their login and password available to their laboratory personnel. The password is reset at the beginning of each fiscal year (on or about July 1) and the information emailed to the PI.
CISPro is accessed at:
If the PI is locked out or forgets his/her password, please contact either the UAA CHO or Lab Support Stockroom/ Hazardous Waste Manager for assistance.
#10 Approvals
In accordance with EHSRMSEM, I accept the responsibility for the proper use and disposal of these materials in the laboratory work described above and have assigned chemical safety responsibilities within the laboratory to people with appropriate training and/or experience.
I have reviewed and approve this Standard Operating Procedure.
Click here to enter a date.
PI Signature
Click here to enter a date.
EHSRMSEM Approval
#11 Emergency Phone Numbers
Principle Investigator / Click here to enter text. / Click here to enter text. /EHSRSMEM CHO / Marcy Marino / 907-786-1279 or 716-201-8051
ESHRMSEM Director / Doug Markussen / 907-786-1335
Director of CAS Facility Support / Krystal Haase / 907-786-1264
Radiation Safety Officer / John Moore / 907-786-1268 or 907-830-0669
Building Manager / Click here to enter text. / Click here to enter text. /
EHSRMSEM Safety Officer / Kelly Carothers / 907-786-1178
ALL EMERGENCIES / 911 systems – APD, AFD, UPD / 911
UPD – Non-emergency / Dispatch / 907-786-1120
#12 Documentation of Training and Proficiency
Add additional lines to the table as necessary.
The PI’s initials indicate the below listed persons have been trained and are proficient in this SOP.
I have read and understand the content of this SOP.
Name - Print / Signature / Date / PI’s InitialsAppendix A
Risk Maps Guide Mitigation Efforts
Consequences / Significant / Manage and Monitor Risks (3) / Highest Risk Management and Monitoring (4) / Highest Risk Management and Monitoring (5)Moderate / Acceptable Risks with Monitoring (2) / Manage and Monitor Risks (3) / Highest Risk Management and Monitoring (4)
Minor / Acceptable Risks (1) / Acceptable Risks with Monitoring (2) / Manage and Monitor Risks (3)
Low/Remote / Moderate / High/Certain
Likelihood
Table Legend
Low Hazard Protocol
Moderate Hazard Protocol
Significant Hazard Protocol
Extreme Hazard Protocol
For Numbers (1), (2), (3), (4), (5) see Assessing Risks: Consequences – on the next page
Assessing Risks: Consequences
Service Disruption Affects Funding & Processes / Reputation / Failure to Meet Legal Obligations / People5
Extreme / Total failure of service, extremely expensive
$$$$ / National publicity >3 days Resignations / Multiple Criminal/ Civil Suits, Claims or Fines >$5M / Fatality of 1+ employees or citizens
4
Very High / Serious disruption to service, costly $$$ / National public and press interest and coverage / Litigation, Claim or Fine of
$500K-5M / Serious injury/ disability of 1+ people
3
Medium / Disruption to service, moderately costly
$$ / Local public and press interest and coverage / Litigation, Claim or Fine of
$100K-500K / Major injury to people
2
Low / Some minor impact on service, minor cost
$ / Contained within department by known by entity / Litigation, Claim or Fine of
$10K-100K / Minor injuries to people
1
Negligible / Annoyance, small or no impact on service and costs / Contained within department / Litigation, Claim or Fine of
<$10K / Minor injury to an individual
Assessing Risks: Likelihood
1 or fewer Persons / 1-10Persons / 10-30 Persons / 30-90 Persons / 90+
Persons
Extreme
70-90% / Fatality of 1+ employees or citizens
Very High
50-70% / Serious injury/ disability of 1+ people
Medium
30-50% / Major injury to people
Low
10-30% / Minor injury to people
Negligible
0-10% / Minor injury to an individual
Table Legend
Low Hazard Protocol
Moderate Hazard Protocol
Significant Hazard Protocol
Extreme Hazard Protocol
Lab SOPPage 1 of 20June 2017
Appendix B
Biological Material Hazard Assessment Form
Complete and attach a Biological Material Assessment form to the SOP. One form may be used for all materials/agents with the same potential hazards and protective measures.
Biological Agent(s): Click here to enter text.
(Organisms, Tissues, Fluids, etc.)
#1 Hazard Identification
Identify the hazard of the agent(s) listed above:
Micro-organisms☐BSL1☐BSL2
Body fluids or tissues☐Animal☐Human
Modes of Exposure/Transmission – check all that apply
☐Inhalation☐Ingestion☐Mucous membranes ☐Percutaneous (cut, needlestick, etc.) ☐Skin contact
#2 Bloodborne Pathogen
The Bloodborne Pathogens Standard (29 CFR, Bloodborne Pathogens. - 1910.1030) applies to all occupational exposure to blood or other potentially infectious materials. Blood means human blood, human blood components, and products made from human blood. Bloodborne Pathogens means pathogenic microorganisms that are present in human blood and can cause disease in humans. These pathogens include, but are not limited to, hepatitis B virus (HBV), hepatitis C virus (HCV) and human immunodeficiency virus (HIV). Additionally, “Other Potentially Infectious Materials” (OPIM) are included under this standard. OPIM means (1) The following human body fluids: semen, vaginal secretions, cerebrospinal fluid, synovial fluid, pleural fluid, pericardial fluid, peritoneal fluid, amniotic fluid, saliva in dental procedures, any body fluid that is visibly contaminated with blood, and all body fluids in situations where it is difficult or impossible to differentiate between body fluids; (2) Any unfixed tissue or organ, including cells and cell lines, (other than intact skin) from a human (living or dead); and (3) HIV-containing cell or tissue cultures, organ cultures, and HIV- or HBV-containing culture medium or other solutions; and blood, organs, or other tissues from experimental animals infected with HIV, HBV or HCV. The above additionally applies to non-human primate materials.
To help determine if a worker is at risk for contact with BBP, please check all that apply.
Will any lab personnel:
☐Work with human blood, blood products or body fluids?
☐Work with unfixed human cells (including tissue culture cells and cell lines), human tissues or organs?