Rajiv Gandhi University Of

RAJIV GANDHI UNIVERSITY OF

HEALTH SCIENCES

KARNATAKA, BANGALORE-560041

SYNOPSIS

BY

Dr. DEEPTI DESAI

FOR THE DEGREE OF AYURVEDA VACHASPATHI

M.D. (DOCTOR OF MEDICINE)

IN

RASASHASTRA

TITLE OF THE TOPIC

“PREPARATION AND PHYSICO CHEMICAL ANALYSIS OF

PATRA HARTALA BHASMA AND ITS ANTIMICROBIAL ACTIVITY”

2007

NAME OF THE INSTITUTE

K.L.E. SOCIETY’S

POST GRADUATE STUDIES AND RESEARCH CENTRE

SHRI B.M.K. AYURVEDA MAHAVIDYALAYA

SHAHAPUR – BELGAUM.-590003

FROM

Dr. DEEPTI DESAI

Ist year M.D.Scholar

Department ofRasashastra & Bhaishajya Kalpana

K.L.E.S. Shri B.M.K. Ayurveda Mahavidyalaya

Post GraduateStudies&Research Center..

Shahapur,Belgaum_590003

To :

THE REGISTRAR

RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES

KARNATAKA

JAYANAGAR, 4TH ‘T’ BLOCK, BANGALORE.-41

THROUGH,

The Principal and Head of the Department

Of Rasashastra&Bhaishajya Kalpana.

Post Graduate Studies and Research Center

K.L.E.S. Shri B.M.K. Ayurveda Mahavidyalaya

Shahpur, Belgaum 590 003.

SUB : Submission of the completed proforma of synopsis for

Registration of subject for dissertation.

Respected Sir,

I request you to kindly register the synopsis on below mentioned subject against my name for the submission of dissertation to the Rajiv Gandhi University of Health Sciences, Karnataka, Bangalore for partial fulfillment of postgraduate course in the specialization of Rasashastra M. D. (Ayurveda)

TITLE OF DISSERTATION :

“PREPARATION AND PHYSICOCHEMICAL ANALYSIS OF

PATRA HARTALA BHASMA AND ITS ANTIMICROBIAL ACTIVITY”

Here with I am enclosing the complete proforma for the registration of subject for dissertation.

Thanking You,

Your’s faithfully

Place : Belgaum

Date : (Dr. DEEPTI DESAI)

RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES

KARNATAKA BANGALORE.

PROFORMA FOR REGISTRATION OF SUBJECT FOR DISSERTATION

1.  Name Of The Candidate : Dr. DEEPTI DESAI

And Address 1ST M.D. Scholar

(In Capital Letter) Post Graduate Department of Rasashastra

K.L.E.S. Shri B. M. K. Ayurveda Mahavidyalaya, Shahapur, Belgaum -3, Karnataka.

2.  Name of The Institution : K. L. E. Society’s Shri B.M.K. Ayurved

Mahavidyalaya, Post Graduate Studies and Research Centre, Shahapur, Belgaum – 3 Karnataka.

3.  Course of Study And : Ayurveda Vachaspathi M.D. (Ayurveda)

Subject : In Rasashastra

4.  Date of Admission To

The Course : 15//06/07

TITLE OF DISSERTATION : “PREPARATION AND PHYSICO CHEMICAL

ANALYSIS OF PATRA HARTALA BHASMA

AND ITS ANTIMICROBIAL ACTIVITY”

6. BRIEF RESUME OF THE INTENDED WORK :-

6.1 NEED OF STUDY :

Haratala (Orpiment;Arsenic Trisulphide-AS2 S3) is a compound of

arsenic and sulphur which is yellow Coloured mineral, has been described under Uparas varga in

Rasa shastra it is found in ore form in nature & can also be made artificially. Ayurved text have

three varities of Haratal7 .

i) Patra ,ii)Pinda iii)Tabak.

Patra Haratala is the best amongst all & is used in medicine , It should be in sheets of

golden yellow Colour with luster.Artificially it is prepared by heating arsenic& sulphur together in

anerobic condition. Hartala Bhasma is choisest remedy for various skin disorders .Hartala bhasma

is pungent hot in potency, astrigent in taste, unctuous, & allevates vata& kapha. The study is

intended to purify the good quality of the Patra Hartala, incinerate it ,& then explore the anti

microbial effect of Patra Hartala w.r.t bhasma its Bactriotatic& Bacteriocidal as well as Anti

fungal action against the strains of G ram positive Gram negative Bacteriae & Fungus responsible

for general skin infections.& also to analyse it Physico-chemical properties.This preparation is

made from easily available rawdrugs, economical,& potential in treating the skin disorders.

Considering the above view, my work will be carried out in 3 steps.

1.  Preparation of Patra Hartala bhasma.

2.  Physico-Chemical analysis of Raw,Shodhita, Marita Patra Hartala.

3. Invitro evaluation if its Bactriostatic ,Bactriocidal &Anti fungal activity

against strains of Gram positive, Gram negative Bacteriae & Fungus

responsible for skin disorders.

6.2 REVIEW OF LITERATURE :-

1.  The Grahya Patra Haratala1 should possess the golden colour,it should have glow, bright, shiny,

& should be in flakes form. Such shudha patra Hartala is katu rasatmaka,ushna veerya yukta ,

krimi nashak, kushtaghna, marita patra Hartala is kushtaghna vrana nashaka, rasayana.

Samanya shodhana of Patra Haratala :

1. Grahya Patra Hartala3 is collected ,made into small pieces. It is tied in a pottali.

Kushmand swarasa(Beinancasa hispida) is extracted, Poured in dola yantra & swedana

is done for 3 hrs.

Marana of Patra Hartala :

1.  Shodhita Patra Hartala4 is taken & 3 bhavanas of Palasha moola (Butea monosperma)

kwath & one Bhavana of mahish mutra is given Chakrikas are prepared & subjected to

puta using 10 upalas like this 12 putas are given.Thus prepared Patra Hartala bhasma

is used in kushta ,vrana, arsha.

6.3OBJECTIVES OF THE STUDY

1. Preparation of Patra hartala bhasma as per classics.

2. Physico chemical analysis of raw & finished product

3. Invitro evaluation of Patra hartala bhasma Bacteriostatic,Bacteriocidal & Anti FungalActivity

7. MATERIALS AND METHODS :-

7.1  Source of Data :-

1.  Sample of Patra Hartala will be collected from the market & get authentificated by the experts

in the subjects.

2. Physico-chemical analysis at a recognised laboratory.

2.  The Anti microbial study will be carried out at a recognized laboratory

7.2  METHODOLOGY:

1.  The sample of Patra Haratala which is having grahya lakshanas like golden colour , glow,

bright, shiny, flaky, will be collected.

2. Shodhana of Patra Hartala will be carried out according to text.

3. Required quantity of Patra Hartala will be taken ,made into small pieces and tied in a pottali

.Kushmand swaras will be extracted in dola yantra & swedana will be done for 3 hr

. 4. Marana of shodhita patra hartala will be done by using Palash moola kwath & mahish mutra

(Buffalo Urine) as bhavana dravya. Twelve Putas will be given using ten upalas(cowdungs)

every time.

5. Physical changes of the shodhita patra hartala & marira patra hartala will be observed w.r.t

organoleptic characters texture, colour, odour.

Chemical Analysis:

Quantitative method

1.  Estimation of percentage of Sulphur .

2.  Estimation of percentage of Arsenic.

3.  Loss on drying.

4.  Total ash content.

5.  Acid insoluble ash.

Qualitative method

Trace element analysis.

Instrumental method

Partical size and surface morphology--- by X.R.D method

Antimicrobial Study7: -

Methodology - Microbroth dilution method

Total of 5 micro organisms which included,

Two gram positive bacteria ------Staphylococcus Aureus and E.fecalis

Two gram negative bacteria------E.coli and Pseudamonas

One fungus ------Candida Albicans

will be included to asses the antimicrobial efficacy of the compound The antimicrobial activity will

be carried out by Microbroth Dilution Method. Here serial dilution of the compound will be tested

against fixed density of each bacteria and fungus The results will be expressed asMinimum Inhibitory

Concentration (MIC). After over night incubation , specimen from each tube which shows turbidity

will be inoculated on Blood agar plate. The absence of growth will indicate Bacteriocidal and Anti

Fungal activity of particular concentration of the compound against tested Bacteria and Fungus.

7.3  DOES THE STUDY REQUIRE ANY INVESTIGATIONS OR INTERVENTIONS TO BE CONDUCTED ON PATIENTS OR OTHER HUMANS OR ANIMALS

--- Not required

7.4  HAS ETHICAL CLEARANCE BEEN OBTAINED FROM INSTITUTION IN CASE OF 7.3

--- Not required

8.0  LIST OF REFERENCES :

1. Ambika Dutta Shastri Ras Ratna Sammuchaya .varanasi, Choukamba bharata academy

1995.Page no.73 shloka no. 71

2. Pandit Kashi nath Shastri. Ras tarangini, Delhi, Motilal Banarasidas 11th edition 1979

page no. 247 shloka no 11/19

3. Shastri Ambikadutta shastri Ras Ratna Sammchaya Varanasi 1995 Page no.73 Shloka

no. 3/74

4. Ambika dutta Shastri, Ras Ratna Sammuchaya. Varanasi, Choukambha bharat academy

1995  page no. 74 shloka no. 3/78 –79

5.  Pandit Kashinath Shastri. Ras tarangini , Delhi, Motilal Banarasi das. 11th edition 1979

page no. 253 shloka no. 11/76

6.  Pandit Kashi nath shastri. Ras tarangini. Delhi Motilal Banarasidas.11th edition 1979

page no.252 shloka no. 11/55.

9. SIGNATURE OF THE CANDIDATE :

10. REMARKS OF THE GUIDE . The present study has been selected with the view of

its analysis.Hence this study is herewith recommended for

the approval from the university.

11. NAME AND DESIGNATION OF

11.1 GUIDE : Dr. P.G. Jadar. M.D (RS.&BK)

Asst. Prof.

. Dept. of Rasashastra & B.Kalpana

K.L.E’S Sri. B.M.K Ayurved Mahavidyalaya

P.G. Studies & Research Centre.

Shahapur, Belgaum-590003.

11.2 SIGNATURE

11.3 CO-GUIDE : Dr.Kishor Bhatt.

Proffesor&H.O.D.

Dept. of Microbiology

.Maratha Mandal Dental College.

Belgaum.

11.4 SIGNATURE

11.5 HEAD OF THE DEPARTMENT : Dr.N.M. Hampannavar. M.D (RS.&BK)

Asst. Prof.

H.O.D. Dept. of Rasashastra & B.Kalpana

K.L.E’S Sri. B.M.K Ayurved Mahavidyalaya

P.G. Studies & Research Centre.

Shahapur, Belgaum-590003.

11.6 SIGNATURE

12. REMARKS OF THE

CHAIRMAN AND PRINCIPAL .

12.1 SIGNATURE

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