QUIZ for BIOSAFETY REFRESHER TRAINING ONLINE MODULE

2011-2012

  1. True or False? Principal Investigators, Laboratory Directors and Supervisors are responsible for reviewing their Biosafety Protocols and Standard Operating procedures with their staff on a regular basis and provide additional laboratory-specific training and supervision?
  1. Where can a copy of the Institutional Biosafety Guide, which documents the Institutional policies, procedures, guidelines and recommended biosafety practices be found?
  2. Only in the Biosafety Officer’s Office
  3. Online at the Biosafety web page
  4. In each departmental manager’s office
  5. In the laboratory, as each lab is required to maintain a hard copy within the lab.
  1. Biosafety Levels (BSLs) refer to:
  2. The level of facility in which biological research can take place.
  3. The level of risk posed by organisms in the lab.
  4. The level of authorization(s) that would be required for researchers to perform experiments with Biological materials.
  5. The combination of Facilities, Equipment and Practices which must be utilized in order to properly contain the risks posed by biological materials.
  1. The Decontamination forms that must be submitted with Moving Service Requests are to document:
  2. Whether potential of biological material contamination has been appropriately reduced using proper disinfection methods before movers handle the equipment.
  3. Whether equipment to be moved contains biological materials and how these have been properly secured during a move or surplus.
  4. Whether a piece of equipment has ever potentially been exposed to biological, chemical or radiological hazards and whether special operations may be required to surplus the equipment.
  5. All of the above.
  1. Which proposed recombinant DNA animal experiment does NOT require IBC registration (notification or approval) prior to its initiation at GHSU:
  2. Creation of a new strain of transgenic rodent which is anticipated to be appropriately contained at BSL-1 by cross-breeding one or more strains of transgenic rodent- as long as the parental strains are contained at BSL-1 and don’t contain certain viral elements.
  3. Commissioning of a vendor to create a new strain of transgenic rodent using traditional (non-viral) transgenic technologies- as long as the rodent can appropriately be contained at BSL-1.
  4. Creation of a transgenic zebrafish- as long as the transgenes do not introduce a hazard and the resultant fish can be contained at BSL-1.
  5. Creation of a new strain of transgenic rodent using viral vector technology to introduce your transgene.
  1. True or False? If a PI has received IBC authorization to work with specific lentiviral expression system to express one transgene, and you now want to use this same system to deliver a different transgene, an amendment will need to be submitted to the IBC for review/approval prior to initiation of the experiment?
  1. True or False? PIs need to submit an amendment to their Biosafety Protocol to document new personnel in their laboratories and remove personnel who have departed their laboratories?
  1. True or False ? A Principal Investigator may potentially be held responsible for possession, use or transfer of a Select Agent or Toxin even if they did not purchased it or was not aware that it was in their laboratory.
  1. True or False? Because of the additional risks associated with flow cytometry of unfixed cells and the need to communicate the risks among users and managers of flow cytometry core facilities, a new Biosafety Protocol Schedule has been developed to document the agents to be used to the IBC.
  1. BSL-1 laboratories:
  2. Require smooth, non-organic, non-porous surfaces which permit decontamination.
  3. Do not need to be equipped with handwash facilities
  4. May leave doors open to adjacent public hallways or office areas.
  5. Do not require IBC registration, and therefore do not have to comply with any specific requirements for safety or compliance.