Supplementary figure S3 Multiple sequence alignment using ClustalW software (Thompson et al. 1994) of nitrilases from Gibberella moniliformis (GiMon Nit; GenBank ABF83489), Neurospora crassa OR74A (NeCra Nit; GenBank CAD70472), Aspergillus niger K10 (AsNig Nit1; GenBank ABX75546; Kaplan et al. 2011a), Penicillium marneffei ATCC18224 (PeMar Nit; GenBank XP_002144951) and Aspergillus niger CBS 513.88 (AsNig Nit2; GenBank XP_001397369).
“Clustal co”, clustal consensus; Asterix, identical aa residues; Colon, similar aa residues; Dot, less similar aa residues.
....|....| ....|....| ....|....| ....|....| ....|....| ....|....|
10 20 30 40 50 60
AsNig Nit1 MAPVLKKYKA AAVNAEPGWF NLEESVRRTI HWIDEAGKAG CKFIAFPELW IPGYPYWMWK
GiMon Nit ---MSKSLKV AAIQAEPVWN DLQGGVNKSI GLIQEAAKEG ANVIGYPEVF IPGYPWSIWA
NeCra Nit ---MATTIKV AVTQAEPIWL DLQASIQKAV SLVHEAASNG AKIVAFSETW APGYPGWCWA
AsNig Nit2 -MTASTKVRV AVTQHEPVWL DLHATVDKTC RLIAEAAGNG AQLITFPECW LPGYPAWIWC
PeMar Nit ---MSKIVRV GAVQSEPVWL DLEGSVDKTI SLIEKAAADG VNVLGFPEVW IPGYPWSMWT
Clustal Co . :. .. : ** * :*. : :: : :*. * :.: :.* : **** *
....|....| ....|....| ....|....| ....|....| ....|....| ....|....|
70 80 90 100 110 120
AsNig Nit1 VNYQESLPLL KKYRENSLPS DSDEMRRIRN AARANKIYVS LGYSEVDLA- SLYTTQVMIS
GiMon Nit NSPTENAPWI NEYFKNSMEK ESPEMDQIRA AVREAGVFVV LGYSERYRG- TLYIAQSFID
NeCra Nit RP--VDPALN TKYAYNSLTA NSPEMEQLQQ AAKEDSIAVV IGFSERSSSG SLYIGQAIIS
AsNig Nit2 RP--VDMGLF TTYLKNSLSY DSEHMRRICN AAAQHKITVV LGLSERDGN- SLYIGQCTID
PeMar Nit SAVINNSHII HDYMNNSMRK DSPQMKRIQA AVKEAGMVVV LGYSERDGA- SLYMAQSFID
Clustal Co . * **: :* .* :: *. : * :* ** :** * *.
....|....| ....|....| ....|....| ....|....| ....|....| ....|....|
130 140 150 160 170 180
AsNig Nit1 PSGDILNHRR KIRATHVERL VFGDGTGDTT ESVIQTDIG------RVGHL NCWENMNPFM
GiMon Nit ETGTIVLHRR KIKPTHVERA IYGDGQGESL TNVADTKFG------RVAGL NCWEHTQTLL
NeCra Nit PQGEVALQRR KLKPTHMERT IFGDGSGPDL NCVAELDFGS ELGSIKVGTL NCWEHAQPLL
AsNig Nit2 STGKIVMRRR KMKPTHMERT VFGESSGRSL LNVVDLPIG------KVGAL ACWEHIQPLL
PeMar Nit PSGEIVHHRR KIKPTHIERT IWGEGQAESL TCVIDSPFG------KVGGL NCWEHLQPLL
Clustal Co * : :** *::.**:** ::*:. . * : :* :*. * ***: :.::
....|....| ....|....| ....|....| ....|....| ....|....| ....|....|
190 200 210 220 230 240
AsNig Nit1 KAYAASLGEQ VHVAAWP-LY PGKETLKYPD PFTNVAEANA DLVTPAYAIE TGTYTLAPWQ
GiMon Nit RYYEYSQDVD IHVSSWPSIF PQNVP---EW PYHITPEC-C KAFSHVVSME GACFVLLASQ
NeCra Nit KFHEIQQGVV IHIAMWPPID PYPGV-EFPG LWSMTADG-C QNLSQTFAVE SGAFVLHCTA
AsNig Nit2 KYHTMIQGEE IHVSAWPVLH PHMGG-ES-- LWGMSQEG-G TGASQVYALE SASFVLLTTA
PeMar Nit RYYEYSQGVQ IHIASWPAEF EMPDPKKIAW LYHETGEA-S YRASQFFAIE GQAFVLVASQ
Clustal Co : : . :*:: ** : : : ::* :.*
....|....| ....|....| ....|....| ....|....| ....|....| ....|....|
250 260 270 280 290 300
AsNig Nit1 TITAEGIKLN TPPGKDLEDP HIYNGHGRIF GPDGQNLVPH PD--KDFEGL LFVDIDLDEC
GiMon Nit IMTEENHKKA NVDGYDYTKK SG-GGFSMIF SPFGEELVKP LA--PNEEGI LYADINLEEK
NeCra Nit VCNESGIEAM DTRNGMVFRE PG-GGHSCVI GPDGRRLTQP LADKPSAEGI VYADLDLTRV
AsNig Nit2 VLGPTCVKKM NLSP--PWDT LG-GGASAVI APDGRRLTEP LP--ANEEGF VYADLDLDMI
PeMar Nit ILTEANVERN NLTGNPVTKT PG-GGFSMIF GPDGKPLCEP VD--AGAEAI LTADIDLRDI
Clustal Co : .* . :: .* *. * . *.: : .*::*
....|....| ....|....| ....|....| ....|....| ....|....| ....|....|
310 320 330 340 350 360
AsNig Nit1 HLSKSLADFG GHYMRPDLIR LLVDTNRKDL VVREDRVNGG VEYTRTVDRV GLSTPLDIAN
GiMon Nit YKAKQNLDIV GHYSRPDQLS LRVNKHAAK------P VFFANDL------
NeCra Nit VTNKSFQDIV GHYSRPDLLW LSYDKEKK------D AAVHRN------
AsNig Nit2 LTCRHFVDAC GHYSRPDLLW LGVDTREKTQ HRPEGQAD-N AAYGLDVPSG LVEEEGA---
PeMar Nit DKPKAFIDVV GHYARPDLLS LLVNPTVDK------H VTTMKK------
Clustal Co : * *** *** : * : .
....|.
AsNig Nit1 TVDSEN
GiMon Nit ------
NeCra Nit ------
AsNig Nit3 ------
PeMar Nit ------
Clustal Co
1