Supplementary figure S3 Multiple sequence alignment using ClustalW software (Thompson et al. 1994) of nitrilases from Gibberella moniliformis (GiMon Nit; GenBank ABF83489), Neurospora crassa OR74A (NeCra Nit; GenBank CAD70472), Aspergillus niger K10 (AsNig Nit1; GenBank ABX75546; Kaplan et al. 2011a), Penicillium marneffei ATCC18224 (PeMar Nit; GenBank XP_002144951) and Aspergillus niger CBS 513.88 (AsNig Nit2; GenBank XP_001397369).

“Clustal co”, clustal consensus; Asterix, identical aa residues; Colon, similar aa residues; Dot, less similar aa residues.


....|....| ....|....| ....|....| ....|....| ....|....| ....|....|

10 20 30 40 50 60

AsNig Nit1 MAPVLKKYKA AAVNAEPGWF NLEESVRRTI HWIDEAGKAG CKFIAFPELW IPGYPYWMWK

GiMon Nit ---MSKSLKV AAIQAEPVWN DLQGGVNKSI GLIQEAAKEG ANVIGYPEVF IPGYPWSIWA

NeCra Nit ---MATTIKV AVTQAEPIWL DLQASIQKAV SLVHEAASNG AKIVAFSETW APGYPGWCWA

AsNig Nit2 -MTASTKVRV AVTQHEPVWL DLHATVDKTC RLIAEAAGNG AQLITFPECW LPGYPAWIWC

PeMar Nit ---MSKIVRV GAVQSEPVWL DLEGSVDKTI SLIEKAAADG VNVLGFPEVW IPGYPWSMWT

Clustal Co . :. .. : ** * :*. : :: : :*. * :.: :.* : **** *

....|....| ....|....| ....|....| ....|....| ....|....| ....|....|

70 80 90 100 110 120

AsNig Nit1 VNYQESLPLL KKYRENSLPS DSDEMRRIRN AARANKIYVS LGYSEVDLA- SLYTTQVMIS

GiMon Nit NSPTENAPWI NEYFKNSMEK ESPEMDQIRA AVREAGVFVV LGYSERYRG- TLYIAQSFID

NeCra Nit RP--VDPALN TKYAYNSLTA NSPEMEQLQQ AAKEDSIAVV IGFSERSSSG SLYIGQAIIS

AsNig Nit2 RP--VDMGLF TTYLKNSLSY DSEHMRRICN AAAQHKITVV LGLSERDGN- SLYIGQCTID

PeMar Nit SAVINNSHII HDYMNNSMRK DSPQMKRIQA AVKEAGMVVV LGYSERDGA- SLYMAQSFID

Clustal Co . * **: :* .* :: *. : * :* ** :** * *.

....|....| ....|....| ....|....| ....|....| ....|....| ....|....|

130 140 150 160 170 180

AsNig Nit1 PSGDILNHRR KIRATHVERL VFGDGTGDTT ESVIQTDIG------RVGHL NCWENMNPFM

GiMon Nit ETGTIVLHRR KIKPTHVERA IYGDGQGESL TNVADTKFG------RVAGL NCWEHTQTLL

NeCra Nit PQGEVALQRR KLKPTHMERT IFGDGSGPDL NCVAELDFGS ELGSIKVGTL NCWEHAQPLL

AsNig Nit2 STGKIVMRRR KMKPTHMERT VFGESSGRSL LNVVDLPIG------KVGAL ACWEHIQPLL

PeMar Nit PSGEIVHHRR KIKPTHIERT IWGEGQAESL TCVIDSPFG------KVGGL NCWEHLQPLL

Clustal Co * : :** *::.**:** ::*:. . * : :* :*. * ***: :.::

....|....| ....|....| ....|....| ....|....| ....|....| ....|....|

190 200 210 220 230 240

AsNig Nit1 KAYAASLGEQ VHVAAWP-LY PGKETLKYPD PFTNVAEANA DLVTPAYAIE TGTYTLAPWQ

GiMon Nit RYYEYSQDVD IHVSSWPSIF PQNVP---EW PYHITPEC-C KAFSHVVSME GACFVLLASQ

NeCra Nit KFHEIQQGVV IHIAMWPPID PYPGV-EFPG LWSMTADG-C QNLSQTFAVE SGAFVLHCTA

AsNig Nit2 KYHTMIQGEE IHVSAWPVLH PHMGG-ES-- LWGMSQEG-G TGASQVYALE SASFVLLTTA

PeMar Nit RYYEYSQGVQ IHIASWPAEF EMPDPKKIAW LYHETGEA-S YRASQFFAIE GQAFVLVASQ

Clustal Co : : . :*:: ** : : : ::* :.*

....|....| ....|....| ....|....| ....|....| ....|....| ....|....|

250 260 270 280 290 300

AsNig Nit1 TITAEGIKLN TPPGKDLEDP HIYNGHGRIF GPDGQNLVPH PD--KDFEGL LFVDIDLDEC

GiMon Nit IMTEENHKKA NVDGYDYTKK SG-GGFSMIF SPFGEELVKP LA--PNEEGI LYADINLEEK

NeCra Nit VCNESGIEAM DTRNGMVFRE PG-GGHSCVI GPDGRRLTQP LADKPSAEGI VYADLDLTRV

AsNig Nit2 VLGPTCVKKM NLSP--PWDT LG-GGASAVI APDGRRLTEP LP--ANEEGF VYADLDLDMI

PeMar Nit ILTEANVERN NLTGNPVTKT PG-GGFSMIF GPDGKPLCEP VD--AGAEAI LTADIDLRDI

Clustal Co : .* . :: .* *. * . *.: : .*::*

....|....| ....|....| ....|....| ....|....| ....|....| ....|....|

310 320 330 340 350 360

AsNig Nit1 HLSKSLADFG GHYMRPDLIR LLVDTNRKDL VVREDRVNGG VEYTRTVDRV GLSTPLDIAN

GiMon Nit YKAKQNLDIV GHYSRPDQLS LRVNKHAAK------P VFFANDL------

NeCra Nit VTNKSFQDIV GHYSRPDLLW LSYDKEKK------D AAVHRN------

AsNig Nit2 LTCRHFVDAC GHYSRPDLLW LGVDTREKTQ HRPEGQAD-N AAYGLDVPSG LVEEEGA---

PeMar Nit DKPKAFIDVV GHYARPDLLS LLVNPTVDK------H VTTMKK------

Clustal Co : * *** *** : * : .

....|.

AsNig Nit1 TVDSEN

GiMon Nit ------

NeCra Nit ------

AsNig Nit3 ------

PeMar Nit ------

Clustal Co

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