Custom Antibody Information Form

Please provide the required information.

If you have any questions, please email us: or call us: +333 90 20 54 70

Customer Information

Name:
Company:
Address:
Phone:
Email:

Antigen Information

Target Name:
NCBI Number:
Target Full Length Sequence:
Protein Description (e.g., whether it is a trans-membrane protein, whether it has a signal peptide, etc):
Protein Localization:
Cell-membrane Cytoplasm Nucleus Other:
If your protein is a trans membrane protein, do you know any extracellular domain?
amino acids; Location:
GI Number of proteins you would like the antibody not to recognize (if you don’t specify, we will design the antigen so that the antibody will not recognize the most highly related protein in NCBI):
Would you like to specifically detect a modification on the target protein?
Yes No
If yes, specify which modification:
Did you already try to develop an antibody against this antigen? (or did you use an antibody developed by somebody else?) Yes No
If yes, specify the method used and if the antibody worked well or not:
Do you provide a plasmid containing the full-length cDNA?
Yes (please fill in Supporting Table 1)
No
Is your plasmid optimized for the production of the protein in a specific system?
Yes No
If yes, specify the system:
Do you provide the peptide antigen? Yes No
If yes, please fill in Supporting Table 2
Do you provide the protein antigen? Yes No
If yes, please fill in Supporting Table 3
Which species would you like to use?
Mouse Rat Either one
What application(s) you would use the antibody for?
ELISA WB FC or FACS IF IP IHC or IHF ELISA Sandwich
Other:
If several applications are needed, please mention the preferred one below (if any):
What kind of sample will the antibody be used on?
Additional relevant information regarding the final application, the target protein, the hybridoma development process…
Final amount of purified antibody required:
Strategy for the final hybridoma/clones cell lines storage :
Cell lines storage in both customerfacilities and ProteoGenix facilities (recommended)
Cell linesstoragein ProteoGenix facilities only
Cell linesstoragein customer facilities only

Supporting Table 1: Plasmid with cDNA provided by customers

cDNA sequence (ORF) :
Plasmid sequence:
Cloning strategy:
If the ORF sequence in the plasmid is different from the NCBI sequence, please specify:
Vector description:
Resistance (or other selection marker):
cDNA NCBI number:
Tag:
Tag position: N-terminal C-terminal

QC Standard:

Provided plasmids should have the correct sequence with sequencing report attached.

Plasmid quantity ≥ 5µg and concentration ≥ 10 ng/μl.

(Note: incorrect plasmids may results in additional fees and project delays or cancellation).

Supporting Table 2: Peptide provided by customers

Unmodified peptide
Peptide sequence:
Peptide purity:
Modified peptide
Modified peptide sequence:
Modified peptide purity:
Protein Localization:
Cell-membrane Cytoplasm Nucleus Other:
Unmodified control peptide sequence:
Unmodified control peptide purity:

QC Standard:

For monoclonal projects: ≥ 5 mg peptide, QC report (MS+HPLC), theoretical MW, purity ≥ 80%.

For modification-specific projects: ≥ 10 mg modified peptide, ≥ 10 mg control peptide, QC report (MS+HPLC), theoretical MW, purity ≥ 80%.

Supporting Table 3: Protein provided by customers

Protein Name:
Molecular Weight:
Concentration (≥ 0.5 mg/ml required):
Quantity (≥ 2.5mg required):
Buffer:
Recommended storage condition:
Specify the tag if any:

QC standard: Purity ≥ 70%; quantity ≥2.5 mg; concentration ≥ 0.5 mg/ml.

For liquid samples, a document specifying the purity, concentration and volume is required.

For lyophilized samples, a document specifying the quantity is required.

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