710.LC GRADUATE MOLECULAR BIOLOGY
Posted Material will be at:
FALL 2011
Monday and Wednesday 5:30 - 7:40 PM
Room: 603 HW
Professors: Thomas Schmidt-Glenewinkel (TSG) / Paul Feinstein (PF)
E-mail: (841 HN)
E-mail: (904 HN)
Grading Scheme as follows:
TSG- 50%
PF-Only 5 of 10quizzes count towards your grade: 40% total. Extra Credit quiz 11.
All quizzes given at the beginning of class.
PF-Homework: 10%
Background Textbooks (do not purchase unless you feel it is necessary):
Molecular Biology/Academic Cell Update by David P. Clark
ISBN 978-0-12-378589-3 or any good Molecular Biology textbook
(I like this book as well)
Recombinant DNA: Genes and Genomes - A Short Course, Third Edition (Watson, Recombinant DNA) [Paperback]
James D. Watson
M. Myers, Amy A. Caudy, Jan A. Witkowski
Course Description: This is a graduate level course in Molecular Biology aimed for giving students the ability to design experiments at the bench.
Learning Objectives: Understand genetic manipulation of a cell and the consequences of that manipulation to the functionality of that cell.
Be able to design experiments using the molecular biology learned in class.
Critically read published papers.
Pre-Req: Advanced Biology coursework: i.e. Biology and Biochemistry Majors.
August 29th, 2011( TSG) Lecture 1:
August31st, 2011( TSG) Lecture 2:
Sept 7th, 2011( TSG) Lecture 3:
Sept 12th, 2011 ( TSG) Lecture 4:
Sept 14th, 2011 ( TSG) Lecture 5:
Sept 19th, 2011 ( TSG) Lecture 6:
Sept 21st, 2011 ( TSG) Lecture 7:
Sept 26th, 2011 ( TSG) Lecture 8:
Oct3rd, 2011 ( TSG) Lecture 9:
Oct5th, 2011 ( TSG) Lecture 10:
Oct12th, 2011 ( TSG) Lecture 11:
Oct17th, 2011 ( TSG) Lecture 12:
Oct19th, 2011 ( TSG) Lecture 13:
Oct 24th, 2011 ( TSG) Lecture 14:
Oct 26th, 2011 ( TSG) Midterm Lect15
Oct 31st, 2011(PF) Lecture 16:
What is a Euk Gene?
Nov 2nd, 2011 (PF) Lecture 17 and Quiz 1: 8% of grade ornot graded
PCR, Gene Synthesis, Codon Optimization
Nov 7th, 2011(PF) Lecture 18 and Quiz 2: 8% of grade or not graded
Bacterial gene expression
Nov 9th, 2011(PF) Lecture 19 and Quiz 3: 8% of grade or not graded
Cloning Vectors, lambda gt10, lambda gt11, BACs, YACs
Nov 14th, 2011(PF) Lecture 20 and Quiz 4: 8% of grade or not graded
UAS-GAL4
Nov 16th, 2011(PF) Lecture 21 and Quiz 5: 8% of grade or not graded
TMACl
Nov 21st, 2011(PF) Lecture 22 and Quiz 6: 8% of grade or not graded
Sequencing, Next Gen Sequencing, Transcriptome Mapping
Two hybrid/One hybrid Systems
tetO-rtta (dox) tetO-tta
Nov 23rd, 2011 (PF) Lecture 23 and Quiz 7: 8% of grade or not graded
GFP/other live-cell indicators. Directed Evolution
Nov 28th, 2011 (PF) Lecture 24 and Quiz 8: 8% of grade or not graded
IRES/T2A/Translational (ribosomal) frameshifting
Nov 30th, 2011 (PF) Lecture 25 and Quiz 9: 8% of grade or not graded
Cre/Flp/Flex/BAC-homologous recombination
Dec 5th, 2011(PF) Lecture 26 and Quiz 10: 8% of grade
Ura3 (complete KO of yeast genome)/ Transgenic mice
Dec 7th, 2011(PF) Lecture 27: and Quiz 11: Extra Credit Quiz
Gene-targeting in ES cells/Zn-finger nucleases
Dec 12th, 2011(PF) Lecture 28: Building Genomes/ Recitation for Homework
"Hunter College regards acts of academic dishonesty (e.g., plagiarism, cheating on
examinations, obtaining unfair advantage, and falsification of records and official
documents) as serious offenses against the values of intellectual honesty. The College
is committed to enforcing the CUNY Policy on Academic Integrity and will pursue
cases of academic dishonesty according to the Hunter College Academic Integrity
Procedures." If I find you are dishonest in your lecture work you will be reported.