1

Dr V. RAMAKRISHNA Phone: 09866016961

Principal Investigator, DST – SERB Project Email:

No: VRK/BTBI/YVU/SERB/Call for Quotations/2015-16 Dated: 19th March, 2016

To

The Firms.

Sir/Madam,

Sub: - YVU - DST SERB Project – Sanctioned to Dr V. Ramakrishna – Purchase of

Consumables– Call for quotations - Reg.

Ref: - 1. Sanction order. SR/SO/HS-0040/2011, SERB, Dt.14-5-2012 & 28-5-2012

2. YVU/DEVP/VRK/BT&BI/SERB/Permission/2015-16, dated 19/3/2016.

.*****

You are requested to send your lowest quotations (F.O.R price in INR) in duplicate towards the supply of following consumables listalong with your terms and conditions, so as to reach to “The Registrar, Yogi Vemana University, Vemanapuram, Kadapa-516003” on or before 26thMarch, 2016 by Speed post / Registered post /courier and mention on envelop quotations for Dr. V. RAMAKRISHNA, DST SERB Project and reference.

Quotations calling for Consumables:

A. List of glassware (Borosil or Duron group)

1. Beakers –500ml, 2000ml

2. Storage bottle - 2000ml

3. Pipettes (Graduated) – 1ml, 2ml, 5ml, 10ml, 20ml

4. Glass Pasteur pipettes with long tip – 9 inch

5. Conical Flask with suction vent-1Ltr.

B. List of plastic ware (Tarson/corning)

1. Narrow mouth bottles – Code. 583100, 583110, 583120, 583130, 583140

2. Wash bottles – Code.561120

3. Eppendorf tubes – 1ml and 2 ml capacity

4. Self-venting labelled wash bottles – Code. 562030 for Methanol

5. Pipette storage rack with magnet – Code 161050

6. Micro tip box – Code. 524052, 524053, 524054

7. Micro tips – bulk – 10ul, 200ul Grad, 1000ul Grad

8. Spinwin Microcentrifuge – Code. 1010, 1020

9. Petridishes – aseptic sterile 460051

10. Micro test plate – Code. 941196 (flat)

11. Staining box – 482020, 482040

12. Cage bin and water bottle for animals

13. Membrane filter holder - 500ml capacity 050020

14. Suction pump & Tygon vacuum tubing

15. Serological pipette sterile – 940040, 940000 – 10ml and 25 ml

16. Kim wipes – 370080, 370090

17. Hands on Nitrile examination gloves 9.5’’length – Medium and large

18. Syringe filters (0.2u or 0.450 micron)

19. Falcon Tubes – 10ml and 50 ml capacity

20. Ice buckets – 525160

  1. Syringe filters (0.2 or 0.4 micron size)

C. Cell culture consumables(Eppendorf/Invitrogen)

  1. Tissue culture flasks for adherent cell types – T-25, T-75 and T-150
  2. Tissue culture dishes for adherent cell types – 35X10MM, 60X15MM, 100X20MM
  3. Tissue culture plates for adherent cell types – 6 well plate, 12 well plate, 24 well plate, 96 well plate
  4. Cell imaging plates – 96 well
  5. Eppendorf easypet / Easypet3
  6. Micropipettes set including 0.01 – 1000 ul capacity
  7. Micropipettes variable range – 0.1 – 2.5ul, 2- 20ul, 20-200ul, 200-1000ul
  8. Falcon tubes – 15 ml and 50 ml capacity
  9. Syringe filters (0.2 or 0.4 micron size)

D. Miscellaneous

  1. Filter papers
  2. Gloves - Medium size
  3. Aluminium foil
  4. Paper towels
  5. Syringes and needles: 1 dozen – 2 cc, 1 dozen – 5cc, needles - 200
  6. Cotton rolls
  7. Bench protectors, plastic backed
  8. Masks
  9. Surgical dissection tool box – High quality
  10. Gel rocker or mechanical shaker

E. Chemical list (Merck/Himedia/Fisher)

  1. Dimethyl sulfoxide (DMSO) - Sigma
  2. Fetal Bovine Serum (FBS)
  3. Eagle’s minimal essential medium (EMEM) for SK N SH cells as per ATCC composition (contain Earle's Balanced Salt Solution, non-essential amino acids, 2 mM L-glutamine, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate)
  4. EMEM powder for cell culture
  5. DMEM powder for cell culture
  6. Penicillin - Streptomycin solution
  7. PMSF – phenyl methyl sulfonyl floride - Sigma
  8. Leupeptien - sigma
  9. Pepstain - sigma
  10. PVDF (polyvinylidene difluoride) membrane for western blotting
  11. Trypsin – EDTA solution
  12. Ponceau s
  13. Erythrosin B - Sigma
  14. HEPES buffer
  15. Penicillin-streptomycin solution
  16. Serum free cell freezing medium
  17. Hydrogen peroxide - Sigma
  18. Phenol reagent (Folin-Ciocalteau reagent)
  19. Methanol
  20. Ethanol (Absolute)
  21. Agar powder, bacteriological
  22. DNA topoisomerase II assay kit
  23. Low melting point agarose - Sigma
  24. DTT - Dithiothreitol
  25. DAPI (4',6-diamidino-2-phenylindole) - Sigma
  26. Propidium iodide - Sigma
  27. Luria broth powder
  28. Quercetin - Sigma
  29. Catechin - Sigma
  30. Rutin - sigma
  31. Himedia Antibiotic discs – Cefepine, cefotaxime, ceftazidime, cefotaxime/ceftazidime+clavulanic acid, ceftazidime/ceftazidime+clavulanic acid, cefpirome
  32. Citrisolv solution
  33. Sodium borohydride
  34. Hydrophobic barrier pen
  35. Freund’s complete adjuvant - Sigma
  36. FITC (Fluorescein isothiocyanate) – Sigma
  37. DiOC2 (Diethyloxacarbocyanine) - sigma
  38. Lanocaine (Benzocaine)
  39. ketamine hydrochloride
  40. xylazine hydrochloride
  41. Cinnamaldehyde
  42. Allicin – Sigma
  43. Thymol - Sigma
  44. Commassie brilliant blue

F. Electrophoresis and western blotting requirements (Invitrogen/thermo)

1. Precasted gels Mini (NUPAGE 4-12% BT)) & respected compatible running gel

buffers for Xcell Sure lock

2. PVDF membrane

3. Kodak Scientific Imaging film/Cl-Xposure film

4. Protein markers/ladder – colored and non-colored

5. Superblock Blocking buffer

6. Chemiluminescent(ECL) substrates for detection

7. Restore Western Blot Stripping Buffer

8. Protease inhibitor cocktail

9. BCA Protein Assay Reagent Kit

G. Antibodies

1. DNA topoisomerase IIbeta

2. Ku 70 and Ku 80

3. p53

4. Actin

5. Secondary antibodies – conjugated horse radish peroxidase

(V. RAMAKRISHNA.)