Appendix S1:

Sum of presence/absence MI/ HU fish dataset. Value indicates the number of site captures per region.

Fish Classification / NLH / SAG / ELM / NLM
Alewife (Alosa psuedoharengus) / 8 / 8 / 2 / 7
Black buffalo (Ictiobus niger) / 0 / 4 / 1 / 1
Banded killifish (Fundulus diaphanus) / 19 / 39 / 20 / 25
Black bullhead (Ameirus melas) / 13 / 3 / 8 / 17
Black crappie (Pomoxis nigromaculatus) / 0 / 6 / 5 / 4
Black redhorse (Moxostoma duquesnii) / 0 / 1 / 0 / 0
Blackchin shiner (Moxostoma duquesnii) / 5 / 0 / 1 / 3
Blacknose shiner (Notropis heterolepis) / 18 / 4 / 12 / 15
Blackside darter (Percina maculata) / 0 / 0 / 1 / 0
Blackspot shiner (Notropis atrocaudalis) / 0 / 0 / 1 / 0
Bluegill (Lepomis macrochirus) / 15 / 22 / 34 / 20
Bluegill hybrid / 1 / 0 / 4 / 0
Bluntnose minnow (Pimephales notatus) / 21 / 21 / 21 / 22
Bowfin (Amia calva) / 10 / 22 / 26 / 12
Brassy minnow (Hybognathus hankinsoni) / 0 / 1 / 0 / 0
Brook silverside (Labidesthes sicculus) / 2 / 4 / 6 / 0
Brook stickleback (Culaea inconstans) / 4 / 3 / 1 / 2
Brown bullhead (Ameiurus nebulosus) / 17 / 6 / 17 / 16
Brown trout (Salmo trutta trutta) / 2 / 1 / 1 / 1
Burbot (Lota lota) / 2 / 0 / 0 / 1
Central mudminnow (Umbra limi) / 1 / 2 / 12 / 2
Channel catfish (Ictalurus punctatus) / 0 / 4 / 5 / 1
Channel darter (Percina copelandi) / 0 / 2 / 0 / 1
Chinnok salmon (Oncorhynchus tshawytscha) / 0 / 0 / 3 / 0
Common carp (Cyprinus carpio) / 8 / 20 / 10 / 11
Common shiner (Luxilus cornutus) / 9 / 11 / 8 / 20
Creek chub (Semotilus atromaculatus) / 0 / 3 / 1 / 1
Emerald shiner (Notropis atherinoides) / 16 / 15 / 11 / 10
Fathead minnow (Pimephales promelas) / 1 / 2 / 0 / 1
Finescale dace (Phoxinus Neogaeus) / 0 / 0 / 0 / 1
Flathead catfish (Pylodictis olivaris) / 0 / 0 / 1 / 0
Freshwater drum (Aplodinotus grunniens) / 0 / 4 / 1 / 1
Gizzard shad (Dorosoma cepedianum) / 0 / 21 / 2 / 4
Golden redhorse (Moxostoma erythrurum) / 0 / 2 / 5 / 0
Golden shiner (Notemigonus crysoleucas) / 7 / 1 / 9 / 5
Goldfish (Carassius auratus) / 0 / 4 / 0 / 0
Grass pickerel (Esox americanus) / 0 / 0 / 6 / 3
Greater redhorse (Moxostoma valenciennesi) / 0 / 0 / 1 / 0
Green sunfish (Lepomis cyanellus) / 3 / 5 / 5 / 0
Iowa darter (Etheostoma exile) / 1 / 0 / 0 / 3
Johnny darter (Etheostoma nigrum) / 12 / 8 / 24 / 11
Lake chub (Couesius plumbeus) / 0 / 1 / 0 / 1
Lake chubsucker (Erimyzon sucetta) / 0 / 0 / 2 / 0
Lake whitefish (Coregonus clupeaformis) / 1 / 0 / 1 / 0
Largemouth bass (Micropterus salmoides) / 24 / 33 / 26 / 11
Logperch (Percina caprodes) / 1 / 8 / 1 / 0
Longnose dace (Rhinichthys cataractae) / 0 / 0 / 0 / 3
Longnose darter (Percina nasuta) / 0 / 0 / 0 / 1
Longnose gar (Lepisosteus osseus) / 5 / 18 / 0 / 13
Mimic shiner (Notropis volucellus) / 5 / 4 / 9 / 4
Mottled sculpin (Cottus bairdi) / 2 / 1 / 1 / 2
Ninespine stickleback (Pungitius pungitius) / 4 / 0 / 0 / 3
Northern pearl dace (Margariscus nachtriebi) / 1 / 0 / 0 / 0
Northern pike (Esox lucius) / 5 / 6 / 15 / 4
Northern redbelly dace (Chrosomus eos) / 1 / 0 / 0 / 2
Pirate perch (Aphredoderus sayanus) / 0 / 0 / 3 / 1
Pugnose minnow (Opsopoedus emiliae) / 0 / 0 / 1 / 0
Pugnose shiner (Notropis anogenus) / 4 / 1 / 2 / 7
Pumpkinseed (Lepomis gibbosus) / 18 / 13 / 34 / 15
Quillback (Carpiodes cyprinus) / 0 / 2 / 2 / 0
Rainbow darter (Etheostoma caeruleum) / 1 / 0 / 0 / 0
Redear sunfish (Lepomis microlophus) / 2 / 0 / 4 / 3
Redtail chub (Nocomis effusus) / 0 / 0 / 0 / 1
River redhorse (Moxostoma carinatum) / 1 / 3 / 3 / 0
Rock bass (Ambloplites rupestris) / 27 / 21 / 30 / 33
Round goby (Neogobius melanostomus) / 13 / 15 / 21 / 21
Sand shiner (Notropis ludibundus) / 11 / 5 / 5 / 15
Shorthead redhorse (Moxostoma macrolepidotum) / 0 / 0 / 1 / 0
Shortnose gar (Lepisosteus platostomus) / 0 / 0 / 0 / 1
Silver redhorse (Moxostoma anisurum) / 1 / 1 / 6 / 1
Slimy sculpin (Cottus cognatus) / 0 / 1 / 1 / 2
Smallmouth bass (Micropterus dolomieu) / 23 / 8 / 6 / 30
Spotfin shiner (Cyprinella spiloptera) / 4 / 13 / 11 / 1
Spottail shiner (Notropis hudsonius) / 16 / 20 / 17 / 19
Spotted gar (Lepisosteus oculatus) / 0 / 0 / 0 / 1
Tadpole madtom (Noturus gyrinus) / 1 / 10 / 7 / 1
Tiger muskellunge (Esox Masquinongy X Esox Lucius) / 1 / 0 / 0 / 3
Trout perch (Percopsis omiscomaycus) / 1 / 1 / 0 / 0
Unknown fish spp. / 0 / 1 / 0 / 3
Unknown sculpin / 0 / 0 / 2 / 0
Unknown shiner / 0 / 4 / 2 / 0
Walleye (Stizostedion vitreum) / 1 / 2 / 0 / 3
Warmouth (Lepomis gulosus) / 0 / 0 / 2 / 0
White bass (Morone chrysops) / 0 / 1 / 0 / 1
White crappie (Pomoxis annularis) / 0 / 2 / 2 / 0
White perch (Morone americana) / 0 / 6 / 0 / 0
White sucker (Catostomus commersoni) / 16 / 11 / 18 / 25
Whitemouth shiner (Notropis alborus) / 3 / 0 / 0 / 0
Yellow bullhead (Ameirus natalis) / 0 / 2 / 5 / 2
Yellow perch (Perca flavescens) / 24 / 36 / 16 / 32
Bigmouth Buffalo (Ictiobus cyprinellus) / 1 / 1 / 0 / 0
Brindled Madtom (Noturus miurus) / 0 / 1 / 0 / 0

1

Presence/absence of MI/ HU macroinvertebrate dataset. Value indicates the number of site captures/region.

Class / Order / Family / Taxa / ELM / NLM / NLH / SAG
Arachnida / Arachnida / Arachnida / Acari / 0 / 1 / 0 / 0
Arachnida / Arachnida / Arachnida / Hydracarina / 26 / 47 / 44 / 58
Branchiopoda / Diplostraca / Diplostraca / Cladocera / 9 / 7 / 3 / 5
Entognatha / Collembola / 14 / 20 / 13 / 27
Insecta / Coleoptera / Anthicidae / 1 / 0 / 0 / 2
Insecta / Coleoptera / Carabidae / 0 / 1 / 1 / 1
Insecta / Coleoptera / Chrysomeloidea / Pyrrhalta / 3 / 0 / 1 / 2
Insecta / Coleoptera / Chrysomelidae / Donacia / 1 / 1 / 5 / 0
Insecta / Coleoptera / Curculionidae / Bagous / 0 / 1 / 1 / 1
Insecta / Coleoptera / Curculionidae / Lixellus / 0 / 0 / 0 / 1
Insecta / Coleoptera / Curculionidae / Lixus / 0 / 0 / 1 / 1
Insecta / Coleoptera / Curculionidae / Rhinoncus / 4 / 0 / 0 / 0
Insecta / Coleoptera / Dryopidae / Helichus / 0 / 0 / 1 / 0
Insecta / Coleoptera / Dytiscidae / Agabetes / 3 / 1 / 0 / 4
Insecta / Coleoptera / Dytiscidae / Agabus / 4 / 0 / 2 / 3
Insecta / Coleoptera / Dytiscidae / Celina / 0 / 2 / 1 / 4
Insecta / Coleoptera / Dytiscidae / Copelatus / 2 / 0 / 0 / 0
Insecta / Coleoptera / Dytiscidae / Coptotomus / 5 / 0 / 4 / 3
Insecta / Coleoptera / Dytiscidae / Cybister / 2 / 0 / 0 / 1
Insecta / Coleoptera / Dytiscidae / Desmopachria / 1 / 0 / 0 / 3
Insecta / Coleoptera / Dytiscidae / Dytiscus / 3 / 4 / 3 / 5
Insecta / Coleoptera / Dytiscidae / Eretes / 0 / 1 / 0 / 0
Insecta / Coleoptera / Dytiscidae / Graphoderus / 1 / 0 / 1 / 1
Insecta / Coleoptera / Dytiscidae / Hydaticus / 1 / 2 / 3 / 3
Insecta / Coleoptera / Dytiscidae / Hydroporus/
Hygrotus / 7 / 10 / 6 / 16
Insecta / Coleoptera / Dytiscidae / Hydrotrupes / 0 / 0 / 0 / 1
Insecta / Coleoptera / Dytiscidae / Hydrovatus / 1 / 2 / 3 / 8
Insecta / Coleoptera / Dytiscidae / Ilybius / 1 / 5 / 1 / 5
Insecta / Coleoptera / Dytiscidae / Laccodytes / 0 / 1 / 0 / 0
Insecta / Coleoptera / Dytiscidae / Laccophilus / 17 / 16 / 15 / 18
Insecta / Coleoptera / Dytiscidae / Laccornis / 1 / 0 / 0 / 2
Insecta / Coleoptera / Dytiscidae / Liodessus / 1 / 8 / 3 / 8
Insecta / Coleoptera / Dytiscidae / Matus / 1 / 0 / 1 / 1
Insecta / Coleoptera / Dytiscidae / Nebrioporus / 2 / 0 / 0 / 1
Insecta / Coleoptera / Dytiscidae / Neoporus / 0 / 1 / 0 / 1
Insecta / Coleoptera / Dytiscidae / Neoscutopterus / 0 / 0 / 2 / 0
Insecta / Coleoptera / Dytiscidae / Oreodytes / 0 / 0 / 0 / 2
Insecta / Coleoptera / Dytiscidae / Rhantus / 1 / 1 / 0 / 0
Insecta / Coleoptera / Dytiscidae / Uvarus / 0 / 2 / 1 / 0
Insecta / Coleoptera / Elmidae / Ancyronyx / 0 / 1 / 0 / 1
Insecta / Coleoptera / Elmidae / Atractelmis / 0 / 0 / 0 / 1
Insecta / Coleoptera / Elmidae / Dubiraphia / 1 / 0 / 1 / 2
Insecta / Coleoptera / Elmidae / Gonielmis / 0 / 0 / 0 / 1
Insecta / Coleoptera / Elmidae / Macronychus / 1 / 0 / 0 / 0
Insecta / Coleoptera / Elmidae / Optioservus / 0 / 1 / 0 / 0
Insecta / Coleoptera / Elmidae / Phanocerus / 0 / 0 / 0 / 1
Insecta / Coleoptera / Elmidae / Rhizelmis / 0 / 0 / 0 / 1
Insecta / Coleoptera / Elmidae / Stenelmis / 1 / 1 / 0 / 2
Insecta / Coleoptera / Erirhinidae / Tanysphyrus / 2 / 0 / 0 / 0
Insecta / Coleoptera / Georyssidae / Georyssus / 0 / 0 / 1 / 0
Insecta / Coleoptera / Gyrinidae / Dineutus / 11 / 15 / 18 / 6
Insecta / Coleoptera / Gyrinidae / Gyretes / 1 / 2 / 0 / 0
Insecta / Coleoptera / Gyrinidae / Gyrinus / 10 / 15 / 23 / 26
Insecta / Coleoptera / Haliplidae / Haliplus / 10 / 13 / 20 / 10
Insecta / Coleoptera / Haliplidae / Peltodytes / 15 / 10 / 5 / 21
Insecta / Coleoptera / Helodidae / Prionocyphon / 2 / 0 / 1 / 0
Insecta / Coleoptera / Helophoridae / Helophorus / 5 / 2 / 0 / 3
Insecta / Coleoptera / Histeridae / Neopachylopus / 0 / 0 / 0 / 1
Insecta / Coleoptera / Hydraenidae / Hydraena / 0 / 1 / 0 / 1
Insecta / Coleoptera / Hydrochidae / Hydrochus / 0 / 1 / 2 / 3
Insecta / Coleoptera / Hydrophilidae / Anacaena / 1 / 2 / 2 / 3
Insecta / Coleoptera / Hydrophilidae / Berosus / 2 / 7 / 6 / 16
Insecta / Coleoptera / Hydrophilidae / Crenitis / 1 / 1 / 0 / 2
Insecta / Coleoptera / Hydrophilidae / Cymbiodyta / 1 / 0 / 0 / 0
Insecta / Coleoptera / Hydrophilidae / Derallus / 0 / 0 / 0 / 2
Insecta / Coleoptera / Hydrophilidae / Enochrus / 0 / 5 / 1 / 9
Insecta / Coleoptera / Hydrophilidae / Helobata / 0 / 1 / 0 / 1
Insecta / Coleoptera / Hydrophilidae / Helochares / 3 / 0 / 0 / 0
Insecta / Coleoptera / Hydrophilidae / Helocombus / 0 / 1 / 0 / 0
Insecta / Coleoptera / Hydrophilidae / Hydrobius / 5 / 3 / 0 / 5
Insecta / Coleoptera / Hydrophilidae / Hydrochara / 0 / 0 / 1 / 2
Insecta / Coleoptera / Hydrophilidae / Hydrophilus / 2 / 2 / 2 / 4
Insecta / Coleoptera / Hydrophilidae / Laccobius / 0 / 4 / 2 / 10
Insecta / Coleoptera / Hydrophilidae / Paracymus / 2 / 3 / 1 / 4
Insecta / Coleoptera / Hydrophilidae / Sperchopsis / 0 / 0 / 0 / 1
Insecta / Coleoptera / Hydrophilidae / Sphaeridiinae / 2 / 0 / 0 / 1
Insecta / Coleoptera / Hydrophilidae / Tropisternus / 18 / 14 / 11 / 16
Insecta / Coleoptera / Lampyridae / Lampyridae / 3 / 0 / 1 / 3
Insecta / Coleoptera / Noteridae / Hydrocanthus / 1 / 0 / 1 / 2
Insecta / Coleoptera / Ptiliidae / 0 / 0 / 0 / 1
Insecta / Coleoptera / Ptilodactylidae / Anchytarsus / 1 / 1 / 0 / 2
Insecta / Coleoptera / Salpingidae / 1 / 0 / 2 / 0
Insecta / Coleoptera / Scirtidae / Cyphon / 2 / 2 / 1 / 0
Insecta / Coleoptera / Scirtidae / Sarabandus / 0 / 0 / 0 / 1
Insecta / Coleoptera / Scirtidae / Scirtes / 1 / 0 / 0 / 0
Insecta / Coleoptera / Staphylinidae / 1 / 1 / 2 / 1
Insecta / Coleoptera / Staphylinidae / Stenus / 1 / 0 / 0 / 0
Insecta / Diptera / Ceratopogonidae / Alluaudomyia / 1 / 1 / 0 / 0
Insecta / Diptera / Ceratopogonidae / Atrichopogon / 0 / 3 / 0 / 1
Insecta / Diptera / Ceratopogonidae / Bezzia/Palpomyia / 18 / 42 / 35 / 51
Insecta / Diptera / Ceratopogonidae / Ceratopogon / 3 / 0 / 0 / 0
Insecta / Diptera / Ceratopogonidae / Culicoides / 2 / 5 / 1 / 4
Insecta / Diptera / Ceratopogonidae / Dasyhelea / 0 / 1 / 1 / 1
Insecta / Diptera / Ceratopogonidae / Forcipomyia / 0 / 1 / 0 / 0
Insecta / Diptera / Ceratopogonidae / Leptoconops / 0 / 0 / 0 / 1
Insecta / Diptera / Ceratopogonidae / Palpomyia / 0 / 0 / 0 / 1
Insecta / Diptera / Ceratopogonidae / Probezzia / 5 / 13 / 9 / 10
Insecta / Diptera / Ceratopogonidae / Serromyia / 0 / 1 / 0 / 2
Insecta / Diptera / Ceratopogonidae / Sphaeromias / 0 / 0 / 0 / 1
Insecta / Diptera / Ceratopogonidae / Stilobezzia / 0 / 0 / 0 / 1
Insecta / Diptera / Chaoboridae / Chaoborus / 0 / 0 / 0 / 1
Insecta / Diptera / Chaoboridae / Eucorethra / 0 / 0 / 0 / 1
Insecta / Diptera / Chaoboridae / Mochlonyx / 1 / 0 / 0 / 0
Insecta / Diptera / Chironomidae / Chironominae / 30 / 55 / 45 / 64
Insecta / Diptera / Chironomidae / Orthocladiinae / 27 / 40 / 35 / 44
Insecta / Diptera / Chironomidae / Prodiamesinae / 0 / 1 / 0 / 0
Insecta / Diptera / Chironomidae / Tanypodinae / 28 / 50 / 46 / 55
Insecta / Diptera / Culicidae / Aedes / 0 / 2 / 1 / 1
Insecta / Diptera / Culicidae / Anopheles / 2 / 1 / 6 / 9
Insecta / Diptera / Culicidae / Culex / 0 / 0 / 0 / 1
Insecta / Diptera / Diptera / Brachycera / 0 / 3 / 2 / 3
Insecta / Diptera / Dixidae / Dixella / 1 / 2 / 1 / 0
Insecta / Diptera / Empididae / Chelifera / 0 / 0 / 0 / 1
Insecta / Diptera / Empididae / Hemerodromia / 0 / 2 / 1 / 1
Insecta / Diptera / Sciomyzidae / Sepedon / 2 / 0 / 0 / 0
Insecta / Diptera / Sciomyzidae / Tetanocera / 2 / 2 / 2 / 0
Insecta / Diptera / Simuliidae / Greniera / 2 / 0 / 0 / 0
Insecta / Diptera / Stratiomyidae / Caloparyphus / 0 / 0 / 0 / 1
Insecta / Diptera / Stratiomyidae / Hedriodiscus / 2 / 0 / 0 / 0
Insecta / Diptera / Stratiomyidae / Myxosargus / 1 / 0 / 0 / 0
Insecta / Diptera / Stratiomyidae / Odontomyia/
Hedriodiscus / 3 / 7 / 3 / 5
Insecta / Diptera / Tabanidae / Chrysops / 0 / 4 / 6 / 0
Insecta / Diptera / Tipulidae / Brachypremna / 1 / 0 / 0 / 0
Insecta / Diptera / Tipulidae / Dicranota / 1 / 0 / 0 / 0
Insecta / Diptera / Tipulidae / Prionocera / 1 / 0 / 0 / 0
Insecta / Diptera / Tipulidae / Tipula / 0 / 2 / 2 / 2
Insecta / Ephemeroptera / Ameletidae / Ameletus / 1 / 0 / 2 / 0
Insecta / Ephemeroptera / Baetidae / Acentrella / 0 / 0 / 2 / 0
Insecta / Ephemeroptera / Baetidae / Baetis / 7 / 10 / 9 / 5
Insecta / Ephemeroptera / Baetidae / Callibaetis / 19 / 24 / 23 / 23
Insecta / Ephemeroptera / Baetidae / Procloeon/Centroptilum/Cloeon / 4 / 20 / 30 / 1
Insecta / Ephemeroptera / Baetiscidae / Baetisca / 0 / 2 / 1 / 1
Insecta / Ephemeroptera / Caenidae / Brachycercus / 1 / 4 / 3 / 4
Insecta / Ephemeroptera / Caenidae / Caenis / 22 / 48 / 43 / 58